Amino acid dipepetide frequency for Pasteurella phage AFS-2018a

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.62AlaAla: 5.62 ± 0.806
0.789AlaCys: 0.789 ± 0.295
4.141AlaAsp: 4.141 ± 0.611
8.48AlaGlu: 8.48 ± 1.551
3.254AlaPhe: 3.254 ± 0.498
4.93AlaGly: 4.93 ± 0.557
0.887AlaHis: 0.887 ± 0.256
6.113AlaIle: 6.113 ± 1.067
5.916AlaLys: 5.916 ± 0.966
8.973AlaLeu: 8.973 ± 1.043
1.775AlaMet: 1.775 ± 0.433
3.55AlaAsn: 3.55 ± 0.414
2.071AlaPro: 2.071 ± 0.41
4.733AlaGln: 4.733 ± 0.755
4.733AlaArg: 4.733 ± 0.685
4.141AlaSer: 4.141 ± 0.637
5.226AlaThr: 5.226 ± 0.762
5.423AlaVal: 5.423 ± 0.678
0.986AlaTrp: 0.986 ± 0.253
2.465AlaTyr: 2.465 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
0.493CysAla: 0.493 ± 0.272
0.0CysCys: 0.0 ± 0.0
0.197CysAsp: 0.197 ± 0.142
0.592CysGlu: 0.592 ± 0.281
0.69CysPhe: 0.69 ± 0.288
0.789CysGly: 0.789 ± 0.323
0.197CysHis: 0.197 ± 0.142
0.394CysIle: 0.394 ± 0.194
0.394CysLys: 0.394 ± 0.183
0.69CysLeu: 0.69 ± 0.254
0.099CysMet: 0.099 ± 0.09
0.0CysAsn: 0.0 ± 0.0
0.394CysPro: 0.394 ± 0.186
0.592CysGln: 0.592 ± 0.19
0.296CysArg: 0.296 ± 0.154
0.197CysSer: 0.197 ± 0.142
0.789CysThr: 0.789 ± 0.32
0.394CysVal: 0.394 ± 0.178
0.197CysTrp: 0.197 ± 0.131
0.394CysTyr: 0.394 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
3.648AspAla: 3.648 ± 0.52
0.69AspCys: 0.69 ± 0.231
3.451AspAsp: 3.451 ± 0.616
4.338AspGlu: 4.338 ± 0.42
1.873AspPhe: 1.873 ± 0.428
3.55AspGly: 3.55 ± 0.497
0.296AspHis: 0.296 ± 0.16
5.423AspIle: 5.423 ± 0.581
3.944AspLys: 3.944 ± 0.624
5.423AspLeu: 5.423 ± 0.697
0.986AspMet: 0.986 ± 0.284
2.761AspAsn: 2.761 ± 0.517
1.578AspPro: 1.578 ± 0.345
1.972AspGln: 1.972 ± 0.449
1.38AspArg: 1.38 ± 0.377
2.761AspSer: 2.761 ± 0.597
1.873AspThr: 1.873 ± 0.311
4.536AspVal: 4.536 ± 0.727
0.789AspTrp: 0.789 ± 0.294
2.465AspTyr: 2.465 ± 0.644
0.0AspXaa: 0.0 ± 0.0
Glu
4.733GluAla: 4.733 ± 0.97
0.493GluCys: 0.493 ± 0.246
3.747GluAsp: 3.747 ± 0.578
4.831GluGlu: 4.831 ± 0.899
3.352GluPhe: 3.352 ± 0.487
3.254GluGly: 3.254 ± 0.472
1.479GluHis: 1.479 ± 0.374
7.099GluIle: 7.099 ± 0.863
6.212GluLys: 6.212 ± 1.097
7.592GluLeu: 7.592 ± 0.677
2.465GluMet: 2.465 ± 0.503
3.057GluAsn: 3.057 ± 0.693
1.775GluPro: 1.775 ± 0.365
4.043GluGln: 4.043 ± 0.628
4.437GluArg: 4.437 ± 0.862
4.536GluSer: 4.536 ± 0.723
4.141GluThr: 4.141 ± 0.762
6.015GluVal: 6.015 ± 0.734
1.775GluTrp: 1.775 ± 0.425
2.169GluTyr: 2.169 ± 0.386
0.0GluXaa: 0.0 ± 0.0
Phe
3.254PheAla: 3.254 ± 0.57
0.296PheCys: 0.296 ± 0.175
2.564PheAsp: 2.564 ± 0.544
2.859PheGlu: 2.859 ± 0.536
0.986PhePhe: 0.986 ± 0.256
3.451PheGly: 3.451 ± 0.622
0.493PheHis: 0.493 ± 0.185
1.676PheIle: 1.676 ± 0.273
3.057PheLys: 3.057 ± 0.488
2.169PheLeu: 2.169 ± 0.51
0.493PheMet: 0.493 ± 0.186
3.057PheAsn: 3.057 ± 0.591
1.282PhePro: 1.282 ± 0.288
0.592PheGln: 0.592 ± 0.269
1.183PheArg: 1.183 ± 0.317
2.662PheSer: 2.662 ± 0.418
1.873PheThr: 1.873 ± 0.38
1.775PheVal: 1.775 ± 0.461
1.282PheTrp: 1.282 ± 0.314
1.183PheTyr: 1.183 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
3.55GlyAla: 3.55 ± 0.611
0.493GlyCys: 0.493 ± 0.2
3.352GlyAsp: 3.352 ± 0.562
5.324GlyGlu: 5.324 ± 0.758
3.155GlyPhe: 3.155 ± 0.52
4.831GlyGly: 4.831 ± 0.758
0.986GlyHis: 0.986 ± 0.349
4.24GlyIle: 4.24 ± 0.672
6.902GlyLys: 6.902 ± 0.76
6.31GlyLeu: 6.31 ± 0.998
1.775GlyMet: 1.775 ± 0.346
3.155GlyAsn: 3.155 ± 0.593
0.296GlyPro: 0.296 ± 0.217
2.169GlyGln: 2.169 ± 0.446
2.564GlyArg: 2.564 ± 0.432
4.24GlySer: 4.24 ± 0.587
4.043GlyThr: 4.043 ± 0.625
5.127GlyVal: 5.127 ± 0.624
0.887GlyTrp: 0.887 ± 0.281
2.859GlyTyr: 2.859 ± 0.536
0.0GlyXaa: 0.0 ± 0.0
His
1.676HisAla: 1.676 ± 0.377
0.296HisCys: 0.296 ± 0.17
0.789HisAsp: 0.789 ± 0.341
0.986HisGlu: 0.986 ± 0.291
0.789HisPhe: 0.789 ± 0.26
1.578HisGly: 1.578 ± 0.359
0.887HisHis: 0.887 ± 0.251
1.282HisIle: 1.282 ± 0.393
2.071HisLys: 2.071 ± 0.476
1.38HisLeu: 1.38 ± 0.334
0.099HisMet: 0.099 ± 0.095
1.578HisAsn: 1.578 ± 0.337
0.789HisPro: 0.789 ± 0.297
0.789HisGln: 0.789 ± 0.281
0.69HisArg: 0.69 ± 0.245
1.183HisSer: 1.183 ± 0.236
0.887HisThr: 0.887 ± 0.305
0.887HisVal: 0.887 ± 0.251
0.296HisTrp: 0.296 ± 0.159
0.296HisTyr: 0.296 ± 0.157
0.0HisXaa: 0.0 ± 0.0
Ile
6.212IleAla: 6.212 ± 0.915
0.789IleCys: 0.789 ± 0.28
4.24IleAsp: 4.24 ± 0.564
4.536IleGlu: 4.536 ± 0.625
1.676IlePhe: 1.676 ± 0.376
4.437IleGly: 4.437 ± 0.595
1.183IleHis: 1.183 ± 0.334
3.155IleIle: 3.155 ± 0.542
5.719IleLys: 5.719 ± 0.704
6.015IleLeu: 6.015 ± 0.736
0.986IleMet: 0.986 ± 0.276
3.057IleAsn: 3.057 ± 0.613
2.662IlePro: 2.662 ± 0.477
3.254IleGln: 3.254 ± 0.558
2.662IleArg: 2.662 ± 0.538
4.536IleSer: 4.536 ± 0.85
3.944IleThr: 3.944 ± 0.846
3.845IleVal: 3.845 ± 0.535
1.282IleTrp: 1.282 ± 0.352
1.873IleTyr: 1.873 ± 0.426
0.0IleXaa: 0.0 ± 0.0
Lys
9.466LysAla: 9.466 ± 1.044
0.197LysCys: 0.197 ± 0.142
2.859LysAsp: 2.859 ± 0.528
5.522LysGlu: 5.522 ± 0.78
2.366LysPhe: 2.366 ± 0.465
7.296LysGly: 7.296 ± 0.752
2.564LysHis: 2.564 ± 0.59
3.944LysIle: 3.944 ± 0.695
5.719LysLys: 5.719 ± 0.808
7.001LysLeu: 7.001 ± 0.945
2.268LysMet: 2.268 ± 0.391
3.155LysAsn: 3.155 ± 0.48
2.268LysPro: 2.268 ± 0.458
3.254LysGln: 3.254 ± 0.559
4.338LysArg: 4.338 ± 0.733
3.451LysSer: 3.451 ± 0.568
4.043LysThr: 4.043 ± 0.65
4.634LysVal: 4.634 ± 0.723
1.183LysTrp: 1.183 ± 0.393
2.465LysTyr: 2.465 ± 0.433
0.0LysXaa: 0.0 ± 0.0
Leu
9.17LeuAla: 9.17 ± 0.941
0.986LeuCys: 0.986 ± 0.283
5.423LeuAsp: 5.423 ± 0.606
5.916LeuGlu: 5.916 ± 0.872
2.662LeuPhe: 2.662 ± 0.634
5.226LeuGly: 5.226 ± 1.109
1.676LeuHis: 1.676 ± 0.35
4.733LeuIle: 4.733 ± 0.565
7.789LeuLys: 7.789 ± 0.583
7.001LeuLeu: 7.001 ± 0.905
2.268LeuMet: 2.268 ± 0.498
5.029LeuAsn: 5.029 ± 0.662
4.437LeuPro: 4.437 ± 0.675
3.648LeuGln: 3.648 ± 0.67
5.226LeuArg: 5.226 ± 0.675
6.705LeuSer: 6.705 ± 0.571
5.522LeuThr: 5.522 ± 0.685
5.62LeuVal: 5.62 ± 0.78
1.085LeuTrp: 1.085 ± 0.326
2.169LeuTyr: 2.169 ± 0.434
0.0LeuXaa: 0.0 ± 0.0
Met
2.761MetAla: 2.761 ± 0.659
0.0MetCys: 0.0 ± 0.0
1.183MetAsp: 1.183 ± 0.336
0.887MetGlu: 0.887 ± 0.289
0.394MetPhe: 0.394 ± 0.165
1.873MetGly: 1.873 ± 0.456
0.197MetHis: 0.197 ± 0.135
1.085MetIle: 1.085 ± 0.341
2.169MetLys: 2.169 ± 0.427
2.071MetLeu: 2.071 ± 0.737
0.296MetMet: 0.296 ± 0.186
1.775MetAsn: 1.775 ± 0.364
0.394MetPro: 0.394 ± 0.186
0.69MetGln: 0.69 ± 0.199
0.493MetArg: 0.493 ± 0.262
2.268MetSer: 2.268 ± 0.465
1.578MetThr: 1.578 ± 0.286
1.282MetVal: 1.282 ± 0.298
0.099MetTrp: 0.099 ± 0.087
0.197MetTyr: 0.197 ± 0.136
0.0MetXaa: 0.0 ± 0.0
Asn
3.944AsnAla: 3.944 ± 0.475
0.493AsnCys: 0.493 ± 0.207
3.254AsnAsp: 3.254 ± 0.453
3.451AsnGlu: 3.451 ± 0.495
1.676AsnPhe: 1.676 ± 0.395
4.338AsnGly: 4.338 ± 0.578
1.282AsnHis: 1.282 ± 0.286
3.352AsnIle: 3.352 ± 0.574
2.564AsnLys: 2.564 ± 0.638
3.944AsnLeu: 3.944 ± 0.641
0.69AsnMet: 0.69 ± 0.219
1.676AsnAsn: 1.676 ± 0.318
2.465AsnPro: 2.465 ± 0.532
2.859AsnGln: 2.859 ± 0.599
2.662AsnArg: 2.662 ± 0.464
2.761AsnSer: 2.761 ± 0.539
2.564AsnThr: 2.564 ± 0.493
3.451AsnVal: 3.451 ± 0.475
0.592AsnTrp: 0.592 ± 0.199
1.38AsnTyr: 1.38 ± 0.312
0.0AsnXaa: 0.0 ± 0.0
Pro
2.268ProAla: 2.268 ± 0.407
0.197ProCys: 0.197 ± 0.116
1.873ProAsp: 1.873 ± 0.375
3.451ProGlu: 3.451 ± 0.462
0.592ProPhe: 0.592 ± 0.192
0.0ProGly: 0.0 ± 0.0
0.986ProHis: 0.986 ± 0.251
2.564ProIle: 2.564 ± 0.558
2.958ProLys: 2.958 ± 0.49
2.169ProLeu: 2.169 ± 0.498
0.493ProMet: 0.493 ± 0.234
1.676ProAsn: 1.676 ± 0.455
1.085ProPro: 1.085 ± 0.318
1.972ProGln: 1.972 ± 0.445
1.479ProArg: 1.479 ± 0.464
1.972ProSer: 1.972 ± 0.451
2.859ProThr: 2.859 ± 0.475
2.071ProVal: 2.071 ± 0.413
0.296ProTrp: 0.296 ± 0.159
0.887ProTyr: 0.887 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
4.338GlnAla: 4.338 ± 0.61
0.099GlnCys: 0.099 ± 0.101
1.676GlnAsp: 1.676 ± 0.324
3.648GlnGlu: 3.648 ± 0.628
2.268GlnPhe: 2.268 ± 0.373
3.747GlnGly: 3.747 ± 0.522
0.887GlnHis: 0.887 ± 0.34
2.859GlnIle: 2.859 ± 0.53
2.662GlnLys: 2.662 ± 0.49
3.845GlnLeu: 3.845 ± 0.599
1.479GlnMet: 1.479 ± 0.287
1.479GlnAsn: 1.479 ± 0.388
1.085GlnPro: 1.085 ± 0.305
2.958GlnGln: 2.958 ± 0.502
1.873GlnArg: 1.873 ± 0.461
2.761GlnSer: 2.761 ± 0.391
2.268GlnThr: 2.268 ± 0.484
2.564GlnVal: 2.564 ± 0.533
0.592GlnTrp: 0.592 ± 0.269
1.972GlnTyr: 1.972 ± 0.343
0.0GlnXaa: 0.0 ± 0.0
Arg
3.352ArgAla: 3.352 ± 0.506
0.493ArgCys: 0.493 ± 0.208
2.958ArgAsp: 2.958 ± 0.484
4.437ArgGlu: 4.437 ± 0.731
2.268ArgPhe: 2.268 ± 0.495
2.268ArgGly: 2.268 ± 0.478
0.789ArgHis: 0.789 ± 0.272
2.662ArgIle: 2.662 ± 0.499
4.043ArgLys: 4.043 ± 0.885
5.324ArgLeu: 5.324 ± 0.781
0.69ArgMet: 0.69 ± 0.266
3.254ArgAsn: 3.254 ± 0.541
1.775ArgPro: 1.775 ± 0.399
1.479ArgGln: 1.479 ± 0.426
1.972ArgArg: 1.972 ± 0.362
2.366ArgSer: 2.366 ± 0.442
2.564ArgThr: 2.564 ± 0.46
2.465ArgVal: 2.465 ± 0.468
0.592ArgTrp: 0.592 ± 0.256
1.676ArgTyr: 1.676 ± 0.396
0.0ArgXaa: 0.0 ± 0.0
Ser
5.127SerAla: 5.127 ± 0.724
0.197SerCys: 0.197 ± 0.136
3.845SerAsp: 3.845 ± 0.655
4.437SerGlu: 4.437 ± 0.679
2.071SerPhe: 2.071 ± 0.48
3.451SerGly: 3.451 ± 0.691
1.085SerHis: 1.085 ± 0.29
3.55SerIle: 3.55 ± 0.679
4.634SerLys: 4.634 ± 0.831
6.902SerLeu: 6.902 ± 0.791
1.183SerMet: 1.183 ± 0.347
3.944SerAsn: 3.944 ± 0.713
2.268SerPro: 2.268 ± 0.45
1.873SerGln: 1.873 ± 0.4
2.761SerArg: 2.761 ± 0.634
3.254SerSer: 3.254 ± 0.536
3.155SerThr: 3.155 ± 0.405
3.254SerVal: 3.254 ± 0.47
0.394SerTrp: 0.394 ± 0.164
1.775SerTyr: 1.775 ± 0.452
0.0SerXaa: 0.0 ± 0.0
Thr
4.634ThrAla: 4.634 ± 0.794
0.296ThrCys: 0.296 ± 0.163
2.662ThrAsp: 2.662 ± 0.537
5.029ThrGlu: 5.029 ± 0.806
2.268ThrPhe: 2.268 ± 0.544
4.24ThrGly: 4.24 ± 0.692
1.085ThrHis: 1.085 ± 0.403
4.831ThrIle: 4.831 ± 0.698
3.944ThrLys: 3.944 ± 0.649
4.141ThrLeu: 4.141 ± 0.899
1.183ThrMet: 1.183 ± 0.323
2.958ThrAsn: 2.958 ± 0.527
2.268ThrPro: 2.268 ± 0.409
2.662ThrGln: 2.662 ± 0.473
2.564ThrArg: 2.564 ± 0.444
2.465ThrSer: 2.465 ± 0.583
3.55ThrThr: 3.55 ± 0.539
3.648ThrVal: 3.648 ± 0.752
0.493ThrTrp: 0.493 ± 0.21
1.578ThrTyr: 1.578 ± 0.404
0.0ThrXaa: 0.0 ± 0.0
Val
6.113ValAla: 6.113 ± 0.543
0.394ValCys: 0.394 ± 0.209
2.761ValAsp: 2.761 ± 0.671
5.226ValGlu: 5.226 ± 0.833
1.972ValPhe: 1.972 ± 0.399
4.24ValGly: 4.24 ± 0.604
0.789ValHis: 0.789 ± 0.279
4.831ValIle: 4.831 ± 0.777
5.423ValLys: 5.423 ± 0.72
6.212ValLeu: 6.212 ± 1.016
0.887ValMet: 0.887 ± 0.282
2.268ValAsn: 2.268 ± 0.501
1.676ValPro: 1.676 ± 0.416
2.958ValGln: 2.958 ± 0.416
3.55ValArg: 3.55 ± 0.437
3.845ValSer: 3.845 ± 0.771
3.55ValThr: 3.55 ± 0.517
2.859ValVal: 2.859 ± 0.508
0.789ValTrp: 0.789 ± 0.237
2.366ValTyr: 2.366 ± 0.426
0.0ValXaa: 0.0 ± 0.0
Trp
1.38TrpAla: 1.38 ± 0.312
0.197TrpCys: 0.197 ± 0.157
0.592TrpAsp: 0.592 ± 0.23
0.986TrpGlu: 0.986 ± 0.302
0.592TrpPhe: 0.592 ± 0.233
0.789TrpGly: 0.789 ± 0.284
0.69TrpHis: 0.69 ± 0.251
0.986TrpIle: 0.986 ± 0.349
0.69TrpLys: 0.69 ± 0.316
1.775TrpLeu: 1.775 ± 0.461
0.493TrpMet: 0.493 ± 0.175
0.197TrpAsn: 0.197 ± 0.121
0.0TrpPro: 0.0 ± 0.0
0.887TrpGln: 0.887 ± 0.395
0.789TrpArg: 0.789 ± 0.25
1.183TrpSer: 1.183 ± 0.318
0.69TrpThr: 0.69 ± 0.27
1.183TrpVal: 1.183 ± 0.407
0.099TrpTrp: 0.099 ± 0.111
0.099TrpTyr: 0.099 ± 0.092
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.465TyrAla: 2.465 ± 0.45
0.394TyrCys: 0.394 ± 0.226
2.169TyrAsp: 2.169 ± 0.456
2.268TyrGlu: 2.268 ± 0.49
1.282TyrPhe: 1.282 ± 0.371
1.972TyrGly: 1.972 ± 0.446
0.789TyrHis: 0.789 ± 0.23
1.282TyrIle: 1.282 ± 0.389
1.282TyrLys: 1.282 ± 0.313
3.352TyrLeu: 3.352 ± 0.495
0.986TyrMet: 0.986 ± 0.26
1.775TyrAsn: 1.775 ± 0.415
1.282TyrPro: 1.282 ± 0.358
1.676TyrGln: 1.676 ± 0.322
1.775TyrArg: 1.775 ± 0.423
1.873TyrSer: 1.873 ± 0.514
1.38TyrThr: 1.38 ± 0.378
1.676TyrVal: 1.676 ± 0.496
0.592TyrTrp: 0.592 ± 0.237
0.69TyrTyr: 0.69 ± 0.27
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (10143 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski