Lysobacter psychrotolerans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8T0U1|A0A3M8T0U1_9GAMM Serine hydroxymethyltransferase OS=Lysobacter psychrotolerans OX=1327343 GN=glyA PE=3 SV=1
MM1 pKa = 7.46LACNGVPEE9 pKa = 4.39HH10 pKa = 6.61VGAVAASDD18 pKa = 3.06IAEE21 pKa = 4.46EE22 pKa = 4.41FTHH25 pKa = 7.03RR26 pKa = 11.84PWHH29 pKa = 6.01QNVQSTWDD37 pKa = 3.56GSRR40 pKa = 11.84LLLQADD46 pKa = 3.67NDD48 pKa = 3.92YY49 pKa = 11.57DD50 pKa = 4.37SDD52 pKa = 4.0GSALADD58 pKa = 3.61EE59 pKa = 5.24FSDD62 pKa = 5.92AIAACIADD70 pKa = 4.37GFNGSITVEE79 pKa = 4.33SVTALAGAA87 pKa = 4.4

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8SNG7|A0A3M8SNG7_9GAMM ATP-dependent protease ATPase subunit HslU OS=Lysobacter psychrotolerans OX=1327343 GN=hslU PE=3 SV=1
MM1 pKa = 7.35SAGRR5 pKa = 11.84RR6 pKa = 11.84LALRR10 pKa = 11.84AIVWQAGATALAVLAFLILRR30 pKa = 11.84GAPSALAAAVGGLAVVSAAWVSARR54 pKa = 11.84VALGGGVQPAGVAVGRR70 pKa = 11.84LLLGVVLKK78 pKa = 9.39WVVVIAVLAMGFAGFHH94 pKa = 5.61VPPLPMLVGVLAATLALLPANLLNRR119 pKa = 4.24

Molecular weight: 11.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3253

0

3253

1113829

38

6492

342.4

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.139 ± 0.061

0.868 ± 0.012

6.077 ± 0.034

5.107 ± 0.042

3.339 ± 0.025

8.734 ± 0.044

2.331 ± 0.022

3.977 ± 0.027

2.459 ± 0.036

10.573 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.079 ± 0.022

2.53 ± 0.031

5.418 ± 0.033

3.618 ± 0.03

7.838 ± 0.052

5.368 ± 0.036

5.107 ± 0.046

7.608 ± 0.032

1.537 ± 0.02

2.296 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski