Anas platyrhynchos (Mallard) (Anas boschas)
Average proteome isoelectric point is 6.91
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 16592 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R0L6Z3|R0L6Z3_ANAPL Peripheral-type benzodiazepine receptor-associated protein 1 OS=Anas platyrhynchos OX=8839 GN=Anapl_03863 PE=4 SV=1
MM1 pKa = 7.46 TSGDD5 pKa = 3.86 GLDD8 pKa = 3.56 SRR10 pKa = 11.84 SAAEE14 pKa = 4.25 LRR16 pKa = 11.84 LDD18 pKa = 4.19 SLYY21 pKa = 10.25 LQCMDD26 pKa = 3.14 SHH28 pKa = 6.3 EE29 pKa = 4.61 ADD31 pKa = 3.43 VAGALPKK38 pKa = 10.28 QEE40 pKa = 4.08 IQSSSEE46 pKa = 3.95 CQAAASDD53 pKa = 4.27 AEE55 pKa = 4.35 VLRR58 pKa = 11.84 PAKK61 pKa = 10.27 AISEE65 pKa = 4.61 PYY67 pKa = 6.41 TTPKK71 pKa = 10.26 IPLSANAPGTDD82 pKa = 3.48 ADD84 pKa = 3.91 STEE87 pKa = 4.59 CEE89 pKa = 4.2 VGLADD94 pKa = 5.55 AYY96 pKa = 11.12 LSPTADD102 pKa = 3.11 SCEE105 pKa = 4.03 NTTLSPADD113 pKa = 4.0 KK114 pKa = 10.99 GDD116 pKa = 3.62 IPHH119 pKa = 6.88 SIVYY123 pKa = 10.0 QNEE126 pKa = 3.61 EE127 pKa = 4.41 GKK129 pKa = 9.57 WVTDD133 pKa = 3.51 LAYY136 pKa = 9.07 YY137 pKa = 10.17 TSFDD141 pKa = 3.77 EE142 pKa = 4.89 EE143 pKa = 3.94 QDD145 pKa = 3.72 LNLAEE150 pKa = 5.11 DD151 pKa = 4.21 DD152 pKa = 5.41 KK153 pKa = 11.41 IDD155 pKa = 3.94 EE156 pKa = 4.7 DD157 pKa = 5.51 FITT160 pKa = 4.32
Molecular weight: 17.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 1.863
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|R0L4W6|R0L4W6_ANAPL Uncharacterized protein OS=Anas platyrhynchos OX=8839 GN=Anapl_16233 PE=4 SV=1
LL1 pKa = 7.39 AGPTASTAVSVVPVGVRR18 pKa = 11.84 AAIARR23 pKa = 11.84 TKK25 pKa = 10.58 GSTIARR31 pKa = 11.84 QAPVAQGGASIARR44 pKa = 11.84 QAPVAQGRR52 pKa = 11.84 APRR55 pKa = 11.84 AIQTPVAQGRR65 pKa = 11.84 AA66 pKa = 3.52
Molecular weight: 6.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16574
18
16592
6979335
19
32970
420.6
46.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.856 ± 0.018
2.476 ± 0.016
4.677 ± 0.015
6.859 ± 0.03
3.747 ± 0.017
6.124 ± 0.026
2.654 ± 0.012
4.64 ± 0.017
6.17 ± 0.024
9.704 ± 0.029
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.157 ± 0.009
3.915 ± 0.014
5.627 ± 0.024
4.863 ± 0.024
5.385 ± 0.018
8.449 ± 0.027
5.489 ± 0.018
6.105 ± 0.024
1.253 ± 0.006
2.734 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here