Hydrocarboniclastica marina

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Alteromonadaceae; Hydrocarboniclastica

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3673 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P7XIX0|A0A4P7XIX0_9ALTE Crotonase/enoyl-CoA hydratase family protein OS=Hydrocarboniclastica marina OX=2259620 GN=soil367_12535 PE=3 SV=1
MM1 pKa = 7.61TFRR4 pKa = 11.84PCVTALALAIAATSAGVQAKK24 pKa = 8.96EE25 pKa = 4.33KK26 pKa = 10.43NDD28 pKa = 4.31DD29 pKa = 3.75IIKK32 pKa = 9.78ISEE35 pKa = 3.9WNYY38 pKa = 9.23EE39 pKa = 3.7ALYY42 pKa = 10.95DD43 pKa = 4.45KK44 pKa = 11.39GGIQADD50 pKa = 3.57EE51 pKa = 4.46LMDD54 pKa = 3.77TEE56 pKa = 4.96VFGSDD61 pKa = 3.23GEE63 pKa = 4.64EE64 pKa = 3.64IGSVEE69 pKa = 4.11NVIIGNDD76 pKa = 3.38NKK78 pKa = 10.25IVAIIAQVGGVWDD91 pKa = 3.49IGDD94 pKa = 3.59THH96 pKa = 6.86VAVPWKK102 pKa = 9.18EE103 pKa = 3.81VKK105 pKa = 8.09MTNDD109 pKa = 3.18GVRR112 pKa = 11.84IPVTEE117 pKa = 4.93DD118 pKa = 2.85NADD121 pKa = 3.45DD122 pKa = 3.75YY123 pKa = 11.87GIFKK127 pKa = 10.81NEE129 pKa = 3.89YY130 pKa = 6.52VTKK133 pKa = 10.85NLDD136 pKa = 3.23QTTKK140 pKa = 10.57VEE142 pKa = 4.32DD143 pKa = 4.47DD144 pKa = 3.56ADD146 pKa = 3.38TGYY149 pKa = 11.01RR150 pKa = 11.84SWKK153 pKa = 8.3LTNLLDD159 pKa = 5.46DD160 pKa = 4.13YY161 pKa = 11.39ATLTGGTGYY170 pKa = 11.03GYY172 pKa = 10.63VDD174 pKa = 3.17NVIFSKK180 pKa = 10.62DD181 pKa = 3.29GKK183 pKa = 9.92IEE185 pKa = 3.99AVVVEE190 pKa = 4.4SASTYY195 pKa = 10.5GAGTYY200 pKa = 9.73AYY202 pKa = 9.03PFYY205 pKa = 10.77GYY207 pKa = 10.63DD208 pKa = 3.46YY209 pKa = 10.12GWNPGYY215 pKa = 10.23DD216 pKa = 3.73AYY218 pKa = 10.48EE219 pKa = 3.94LPYY222 pKa = 10.75EE223 pKa = 4.25EE224 pKa = 5.86NEE226 pKa = 4.34VADD229 pKa = 5.67LDD231 pKa = 3.63IFDD234 pKa = 4.69YY235 pKa = 11.81NEE237 pKa = 5.66FDD239 pKa = 5.5DD240 pKa = 3.91TWDD243 pKa = 3.31WDD245 pKa = 3.77SS246 pKa = 3.6

Molecular weight: 27.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P7XCM8|A0A4P7XCM8_9ALTE Uncharacterized protein OS=Hydrocarboniclastica marina OX=2259620 GN=soil367_00530 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVIKK11 pKa = 10.52RR12 pKa = 11.84KK13 pKa = 8.26RR14 pKa = 11.84THH16 pKa = 5.92GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84KK29 pKa = 9.07VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.73RR41 pKa = 11.84LCAA44 pKa = 3.9

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3673

0

3673

1223267

23

3583

333.0

36.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.367 ± 0.043

0.941 ± 0.013

5.698 ± 0.037

6.474 ± 0.041

3.737 ± 0.028

7.897 ± 0.04

2.203 ± 0.02

4.963 ± 0.033

3.352 ± 0.035

10.921 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.019

3.057 ± 0.02

4.766 ± 0.026

4.157 ± 0.031

6.797 ± 0.038

6.044 ± 0.028

5.046 ± 0.028

7.285 ± 0.035

1.37 ± 0.017

2.542 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski