Abiotrophia defectiva ATCC 49176
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1943 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W1Q3S0|W1Q3S0_ABIDE Uncharacterized protein OS=Abiotrophia defectiva ATCC 49176 OX=592010 GN=GCWU000182_000871 PE=4 SV=1
MM1 pKa = 7.51 NPYY4 pKa = 10.06 QDD6 pKa = 5.56 YY7 pKa = 9.72 VQDD10 pKa = 3.73 WQAQAPDD17 pKa = 4.1 YY18 pKa = 10.51 PSQALLQVASDD29 pKa = 4.81 LYY31 pKa = 10.18 QQQEE35 pKa = 3.82 DD36 pKa = 3.95 RR37 pKa = 11.84 LRR39 pKa = 11.84 QLEE42 pKa = 4.09 GQLDD46 pKa = 3.82 GSMWSPKK53 pKa = 10.07 DD54 pKa = 3.3 WQNN57 pKa = 3.08
Molecular weight: 6.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.808
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 1.901
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|W1Q4G4|W1Q4G4_ABIDE LemA family protein OS=Abiotrophia defectiva ATCC 49176 OX=592010 GN=GCWU000182_000472 PE=3 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 9.67 QPKK8 pKa = 9.36 KK9 pKa = 7.48 RR10 pKa = 11.84 TRR12 pKa = 11.84 KK13 pKa = 9.06 KK14 pKa = 8.41 VHH16 pKa = 5.85 GFRR19 pKa = 11.84 KK20 pKa = 10.01 RR21 pKa = 11.84 MSTKK25 pKa = 10.14 NGRR28 pKa = 11.84 NVLKK32 pKa = 10.51 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LAQQ44 pKa = 3.57
Molecular weight: 5.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1943
0
1943
613071
39
4231
315.5
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.891 ± 0.116
0.6 ± 0.018
5.67 ± 0.054
6.366 ± 0.057
3.966 ± 0.045
6.824 ± 0.063
2.073 ± 0.035
6.025 ± 0.061
5.481 ± 0.062
10.589 ± 0.112
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.399 ± 0.031
3.801 ± 0.048
3.928 ± 0.058
5.455 ± 0.068
4.461 ± 0.049
5.961 ± 0.056
5.414 ± 0.091
7.201 ± 0.068
1.112 ± 0.025
3.781 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here