Bifidobacterium psychraerophilum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2122 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087CDS1|A0A087CDS1_9BIFI Membrane-associated protein OS=Bifidobacterium psychraerophilum OX=218140 GN=BPSY_1829 PE=4 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.82SPRR9 pKa = 11.84NKK11 pKa = 10.84DD12 pKa = 3.11EE13 pKa = 5.7DD14 pKa = 4.16EE15 pKa = 4.93EE16 pKa = 4.45SLQALGKK23 pKa = 8.0GTPSTSSDD31 pKa = 2.93IDD33 pKa = 3.79DD34 pKa = 5.03DD35 pKa = 4.45EE36 pKa = 4.37NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 4.92LSNEE53 pKa = 4.01DD54 pKa = 3.61SSVTVIPMQGDD65 pKa = 3.54EE66 pKa = 5.47FICSVCFLVKK76 pKa = 10.28HH77 pKa = 6.38RR78 pKa = 11.84SQLAYY83 pKa = 8.32MTDD86 pKa = 3.7DD87 pKa = 4.26GEE89 pKa = 4.55PVCKK93 pKa = 10.28EE94 pKa = 3.89CAAA97 pKa = 4.21

Molecular weight: 10.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087CHS5|A0A087CHS5_9BIFI TetR family transcriptional regulator OS=Bifidobacterium psychraerophilum OX=218140 GN=BPSY_0616 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 9.49GRR39 pKa = 11.84KK40 pKa = 8.88SLSAA44 pKa = 3.86

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2122

0

2122

762520

29

3111

359.3

39.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.645 ± 0.055

0.83 ± 0.015

6.256 ± 0.045

5.499 ± 0.053

3.442 ± 0.028

8.206 ± 0.041

2.28 ± 0.027

5.58 ± 0.041

3.482 ± 0.039

9.247 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.024

3.054 ± 0.032

4.436 ± 0.028

3.738 ± 0.03

5.913 ± 0.06

7.447 ± 0.058

5.902 ± 0.048

7.618 ± 0.044

1.353 ± 0.02

2.575 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski