Urechidicola croceus
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1D8P9D9|A0A1D8P9D9_9FLAO Acyl-CoA thioesterase OS=Urechidicola croceus OX=1850246 GN=LPB138_11090 PE=3 SV=1
MM1 pKa = 6.91 VFSVGFSQTVIYY13 pKa = 10.95 SEE15 pKa = 4.92 DD16 pKa = 3.57 FEE18 pKa = 6.27 SNIGTWTQDD27 pKa = 3.09 TADD30 pKa = 3.74 TLDD33 pKa = 2.92 WSRR36 pKa = 11.84 NSGGTGSGGTGPNNSSDD53 pKa = 3.12 GSFYY57 pKa = 10.3 MYY59 pKa = 10.59 TEE61 pKa = 4.29 ASSNYY66 pKa = 7.75 NTTANFISEE75 pKa = 4.41 PFNLSGISSPIFTFHH90 pKa = 5.98 YY91 pKa = 10.82 HH92 pKa = 5.16 MFGSNMGTLNVNISTDD108 pKa = 3.24 GGSTFGAPIWTQNGQHH124 pKa = 5.86 QGSNSAAWQQVDD136 pKa = 3.81 IDD138 pKa = 4.0 LSTYY142 pKa = 10.17 IGQTINIQFNGQVGSSYY159 pKa = 10.81 RR160 pKa = 11.84 SDD162 pKa = 3.0 IAIDD166 pKa = 4.36 EE167 pKa = 4.38 ISLTVTSSPDD177 pKa = 3.28 PEE179 pKa = 4.39 IDD181 pKa = 3.39 VTGNSLPIIGDD192 pKa = 3.48 GTNAPSINDD201 pKa = 3.26 NTDD204 pKa = 3.13 FGTVQVGSEE213 pKa = 4.21 TSIKK217 pKa = 10.06 TYY219 pKa = 10.37 TITNNGSLDD228 pKa = 3.8 LVLGTSTISGSSDD241 pKa = 3.2 FVISTFPGTTITPGNSEE258 pKa = 4.04 IIEE261 pKa = 4.02 ISFNTLSLGIQTAQLSIVNNDD282 pKa = 3.54 PDD284 pKa = 4.02 EE285 pKa = 4.43 NPYY288 pKa = 10.63 LINLSAEE295 pKa = 4.27 GTQAFYY301 pKa = 11.09 DD302 pKa = 3.71 SDD304 pKa = 4.18 GDD306 pKa = 4.43 GVFDD310 pKa = 6.2 DD311 pKa = 5.63 VDD313 pKa = 3.9 IDD315 pKa = 4.48 DD316 pKa = 5.6 DD317 pKa = 4.34 NDD319 pKa = 4.71 GIIDD323 pKa = 3.77 SLEE326 pKa = 3.9 EE327 pKa = 4.53 SNCSGSIITNSVNYY341 pKa = 10.05 KK342 pKa = 10.08 FLNEE346 pKa = 4.05 TFGTGGRR353 pKa = 11.84 TTINTSYY360 pKa = 11.18 NATTTYY366 pKa = 10.31 CYY368 pKa = 10.68 EE369 pKa = 5.06 DD370 pKa = 3.7 GTAGTDD376 pKa = 3.36 TTGCPTLSDD385 pKa = 3.31 TSLNDD390 pKa = 3.36 GEE392 pKa = 4.5 YY393 pKa = 9.72 TVYY396 pKa = 10.99 SRR398 pKa = 11.84 ITNNDD403 pKa = 3.07 DD404 pKa = 3.16 VADD407 pKa = 5.25 GINVDD412 pKa = 3.33 IASWAEE418 pKa = 3.85 DD419 pKa = 3.37 FWYY422 pKa = 10.09 EE423 pKa = 5.07 GEE425 pKa = 4.24 DD426 pKa = 3.67 HH427 pKa = 6.82 TPGDD431 pKa = 3.32 IDD433 pKa = 3.35 GRR435 pKa = 11.84 MAIFNASYY443 pKa = 10.97 DD444 pKa = 3.4 PGVFYY449 pKa = 9.85 TASITGALPNVPITYY464 pKa = 10.05 DD465 pKa = 3.51 FWVLNIDD472 pKa = 3.77 RR473 pKa = 11.84 TDD475 pKa = 3.58 APCVDD480 pKa = 4.39 GGSTCTGPGVDD491 pKa = 4.4 GSRR494 pKa = 11.84 LRR496 pKa = 11.84 PNVLVEE502 pKa = 4.46 FRR504 pKa = 11.84 DD505 pKa = 3.58 LTGNVLASITTGDD518 pKa = 3.65 IDD520 pKa = 3.9 PTPVGDD526 pKa = 4.42 TAGNWMNFRR535 pKa = 11.84 ADD537 pKa = 3.26 LVLNVSQFEE546 pKa = 4.46 VVFINNEE553 pKa = 4.0 TGGLGNDD560 pKa = 4.49 LALDD564 pKa = 4.65 DD565 pKa = 4.68 IKK567 pKa = 10.81 IEE569 pKa = 4.02 QTLCDD574 pKa = 3.73 VDD576 pKa = 3.56 NDD578 pKa = 4.68 GIADD582 pKa = 3.95 VFDD585 pKa = 5.64 LDD587 pKa = 4.56 SDD589 pKa = 4.03 DD590 pKa = 5.9 DD591 pKa = 5.29 GIVDD595 pKa = 5.61 LIEE598 pKa = 4.47 VGLGALNNGNGVIDD612 pKa = 4.34 PVVGWVDD619 pKa = 3.44 VNGDD623 pKa = 4.0 GLHH626 pKa = 7.0 DD627 pKa = 3.87 AAAGNVIPDD636 pKa = 3.71 TDD638 pKa = 4.01 GDD640 pKa = 4.08 GTPDD644 pKa = 3.88 YY645 pKa = 11.4 LDD647 pKa = 5.04 LDD649 pKa = 4.21 SDD651 pKa = 3.77 NDD653 pKa = 4.41 SIFDD657 pKa = 3.62 VDD659 pKa = 4.19 EE660 pKa = 4.84 SGATNSSMPGFINEE674 pKa = 4.85 DD675 pKa = 3.05 GDD677 pKa = 4.03 INGDD681 pKa = 3.34 GTGNNTEE688 pKa = 4.07 TEE690 pKa = 4.07 AFRR693 pKa = 11.84 EE694 pKa = 4.03 KK695 pKa = 10.6 DD696 pKa = 3.09 TDD698 pKa = 3.66 GDD700 pKa = 4.13 GTSEE704 pKa = 4.48 YY705 pKa = 10.59 YY706 pKa = 10.93 GDD708 pKa = 5.26 GILDD712 pKa = 3.71 IYY714 pKa = 9.83 EE715 pKa = 4.59 HH716 pKa = 7.35 SSGLYY721 pKa = 10.7 GNANQTNPIDD731 pKa = 4.08 SDD733 pKa = 3.76 GDD735 pKa = 4.03 TIPDD739 pKa = 3.68 YY740 pKa = 11.45 LDD742 pKa = 3.47 PTTNTTHH749 pKa = 7.73 DD750 pKa = 3.37 ISTTLYY756 pKa = 11.16 ASLDD760 pKa = 3.78 ANNDD764 pKa = 3.88 GIIDD768 pKa = 3.91 DD769 pKa = 5.11 TIDD772 pKa = 3.34 TDD774 pKa = 4.09 GDD776 pKa = 4.47 GILDD780 pKa = 4.93 LYY782 pKa = 10.66 DD783 pKa = 3.79 TDD785 pKa = 5.71 DD786 pKa = 3.96 MLFGSPRR793 pKa = 11.84 DD794 pKa = 3.63 LDD796 pKa = 3.43 RR797 pKa = 11.84 SLYY800 pKa = 10.18 IDD802 pKa = 3.54 FDD804 pKa = 3.56 GRR806 pKa = 11.84 NDD808 pKa = 3.65 YY809 pKa = 11.26 AQDD812 pKa = 3.37 TSLLGGLTDD821 pKa = 3.65 ATIMAWINIDD831 pKa = 4.93 DD832 pKa = 4.42 LFSNQGFIVGQNNFNLKK849 pKa = 9.91 INNSRR854 pKa = 11.84 NLIATANGTTLSNSTVLNTSQWIHH878 pKa = 4.8 VAAVYY883 pKa = 10.75 SSGTNTFNLYY893 pKa = 10.89 VNGEE897 pKa = 4.28 EE898 pKa = 4.82 VNTTTAASALNVDD911 pKa = 4.01 SSLLTLAKK919 pKa = 10.58 NPSTDD924 pKa = 2.93 TEE926 pKa = 4.48 YY927 pKa = 11.05 FNGGIEE933 pKa = 3.99 EE934 pKa = 4.16 VKK936 pKa = 10.54 IFGIALTEE944 pKa = 4.0 LQLQKK949 pKa = 10.18 MVYY952 pKa = 10.13 QKK954 pKa = 10.39 IEE956 pKa = 4.38 DD957 pKa = 3.55 NGQILGEE964 pKa = 4.83 IIPIDD969 pKa = 4.13 IDD971 pKa = 4.26 VAWLDD976 pKa = 3.31 LLRR979 pKa = 11.84 YY980 pKa = 9.76 YY981 pKa = 11.2 RR982 pKa = 11.84 MDD984 pKa = 4.14 AYY986 pKa = 11.04 QDD988 pKa = 3.96 DD989 pKa = 4.44 VVDD992 pKa = 4.71 NYY994 pKa = 7.81 TTSIIDD1000 pKa = 3.37 QYY1002 pKa = 10.53 PGTFARR1008 pKa = 11.84 CYY1010 pKa = 8.62 NVKK1013 pKa = 10.29 NIKK1016 pKa = 10.23 VEE1018 pKa = 4.04 TAPMPFVTQAVPSNYY1033 pKa = 10.01 DD1034 pKa = 2.58 IGVAVSQNNDD1044 pKa = 2.64 VRR1046 pKa = 11.84 GDD1048 pKa = 3.74 DD1049 pKa = 3.59 VKK1051 pKa = 11.36 DD1052 pKa = 3.67 YY1053 pKa = 10.8 DD1054 pKa = 3.64 WSITHH1059 pKa = 6.52 IRR1061 pKa = 11.84 HH1062 pKa = 7.18 DD1063 pKa = 3.56 INLDD1067 pKa = 3.65 DD1068 pKa = 4.04 FHH1070 pKa = 9.09 SDD1072 pKa = 3.2 LGLMIDD1078 pKa = 3.62 TGVLVNLNNDD1088 pKa = 3.4 TALQNNWYY1096 pKa = 9.98 LNLDD1100 pKa = 3.53 GVVDD1104 pKa = 5.07 LNGEE1108 pKa = 4.2 SQLVQTSEE1116 pKa = 4.57 SILDD1120 pKa = 3.64 IDD1122 pKa = 3.79 SEE1124 pKa = 5.15 GYY1126 pKa = 9.68 IEE1128 pKa = 5.92 RR1129 pKa = 11.84 DD1130 pKa = 3.3 QQGTANSFTYY1140 pKa = 10.21 NYY1142 pKa = 8.07 WSSPVSVIGAGVNNTPFSISDD1163 pKa = 3.5 VLRR1166 pKa = 11.84 DD1167 pKa = 3.77 GTTSSTPINLDD1178 pKa = 2.81 FDD1180 pKa = 4.23 TATTLSTADD1189 pKa = 3.94 PYY1191 pKa = 11.73 YY1192 pKa = 11.13 SDD1194 pKa = 4.61 GALTNPRR1201 pKa = 11.84 KK1202 pKa = 9.51 IASYY1206 pKa = 9.31 WLWKK1210 pKa = 10.05 FVNQTNDD1217 pKa = 3.05 YY1218 pKa = 11.16 ANWEE1222 pKa = 4.0 WFGAYY1227 pKa = 10.43 NNINVADD1234 pKa = 4.32 GFSMKK1239 pKa = 10.22 GVSSTSSVTDD1249 pKa = 3.11 SQNYY1253 pKa = 8.07 VFVGKK1258 pKa = 9.53 PNNAPNVTGEE1268 pKa = 4.2 IVHH1271 pKa = 5.36 TTFPGGTSIEE1281 pKa = 4.53 GYY1283 pKa = 8.84 TYY1285 pKa = 11.38 NSLTGNPFPSAMDD1298 pKa = 3.8 ADD1300 pKa = 4.48 QFILDD1305 pKa = 4.87 NISSTTGTIYY1315 pKa = 10.31 FWEE1318 pKa = 4.16 HH1319 pKa = 5.36 WGGGNHH1325 pKa = 5.69 NWEE1328 pKa = 5.03 DD1329 pKa = 3.56 YY1330 pKa = 9.9 RR1331 pKa = 11.84 AGYY1334 pKa = 10.61 SMYY1337 pKa = 10.08 TMSGGLPGVSHH1348 pKa = 7.88 PDD1350 pKa = 3.26 GANVGGGTKK1359 pKa = 9.69 TPGQYY1364 pKa = 9.96 IPVGQGFYY1372 pKa = 10.19 IVSSNAGGDD1381 pKa = 3.64 VVFKK1385 pKa = 10.66 NSQRR1389 pKa = 11.84 IFKK1392 pKa = 10.34 IEE1394 pKa = 4.46 LDD1396 pKa = 3.85 DD1397 pKa = 4.68 DD1398 pKa = 4.37 TNSDD1402 pKa = 2.99 HH1403 pKa = 7.99 SIFTRR1408 pKa = 11.84 AASKK1412 pKa = 11.28 NNINEE1417 pKa = 4.0 ASTEE1421 pKa = 4.13 RR1422 pKa = 11.84 NNNTTNEE1429 pKa = 4.03 KK1430 pKa = 9.88 QRR1432 pKa = 11.84 IRR1434 pKa = 11.84 LGFEE1438 pKa = 3.92 SPDD1441 pKa = 3.41 GYY1443 pKa = 10.86 HH1444 pKa = 6.08 RR1445 pKa = 11.84 QVLMAFLEE1453 pKa = 4.76 GATDD1457 pKa = 5.43 AIDD1460 pKa = 3.72 PGYY1463 pKa = 10.76 DD1464 pKa = 3.46 GEE1466 pKa = 6.08 AGDD1469 pKa = 4.62 YY1470 pKa = 10.71 LPNDD1474 pKa = 3.61 GFFIQDD1480 pKa = 3.39 DD1481 pKa = 3.96 KK1482 pKa = 11.59 YY1483 pKa = 10.6 YY1484 pKa = 10.92 AIIAFGEE1491 pKa = 3.95 FDD1493 pKa = 4.0 EE1494 pKa = 4.51 EE1495 pKa = 4.8 RR1496 pKa = 11.84 EE1497 pKa = 4.15 VPISIFIDD1505 pKa = 3.56 EE1506 pKa = 5.06 ANDD1509 pKa = 3.67 GGNQKK1514 pKa = 10.3 FMVDD1518 pKa = 3.87 RR1519 pKa = 11.84 IEE1521 pKa = 4.63 NMPEE1525 pKa = 3.85 TISIYY1530 pKa = 10.5 IKK1532 pKa = 10.83 DD1533 pKa = 3.87 NEE1535 pKa = 4.38 TGILHH1540 pKa = 7.36 DD1541 pKa = 3.42 IKK1543 pKa = 11.56 NNVFEE1548 pKa = 4.56 VALSTGEE1555 pKa = 3.93 HH1556 pKa = 5.29 KK1557 pKa = 9.66 TRR1559 pKa = 11.84 FSVVFQDD1566 pKa = 5.16 RR1567 pKa = 11.84 VLSLNAEE1574 pKa = 4.19 LAEE1577 pKa = 4.45 SNDD1580 pKa = 3.15 LKK1582 pKa = 11.57 VFMNNSNSEE1591 pKa = 3.71 IQIINNKK1598 pKa = 8.63 NLEE1601 pKa = 4.24 LNEE1604 pKa = 3.69 ISLYY1608 pKa = 10.96 NSIGQTINSWNHH1620 pKa = 6.03 LGNDD1624 pKa = 3.59 MMISLPLNNISSGVYY1639 pKa = 9.33 IVTIKK1644 pKa = 6.46 TTKK1647 pKa = 10.42 GVVSQKK1653 pKa = 10.63 IIIKK1657 pKa = 10.26
Molecular weight: 179.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.656
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.668
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.821
DTASelect 4.126
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.846
Patrickios 1.024
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1D8PBP7|A0A1D8PBP7_9FLAO PDZ domain-containing protein OS=Urechidicola croceus OX=1850246 GN=LPB138_04700 PE=4 SV=1
MM1 pKa = 7.63 KK2 pKa = 10.41 KK3 pKa = 10.58 GILFIAAMLLLVSVSEE19 pKa = 4.27 ANNNGRR25 pKa = 11.84 INSRR29 pKa = 11.84 MGYY32 pKa = 7.83 TNVYY36 pKa = 9.37 DD37 pKa = 3.85 AQPIQFKK44 pKa = 10.64 EE45 pKa = 3.99 KK46 pKa = 10.38 GIQFYY51 pKa = 11.33 VFLDD55 pKa = 3.93 GQFDD59 pKa = 4.51 FSTRR63 pKa = 11.84 PNRR66 pKa = 11.84 NFNNQYY72 pKa = 10.98 KK73 pKa = 10.15 NLNSRR78 pKa = 11.84 RR79 pKa = 11.84 NLRR82 pKa = 11.84 IINNRR87 pKa = 11.84 GLRR90 pKa = 11.84 IEE92 pKa = 3.89 RR93 pKa = 11.84 DD94 pKa = 3.13 YY95 pKa = 11.07 RR96 pKa = 11.84 GRR98 pKa = 11.84 IRR100 pKa = 11.84 RR101 pKa = 11.84 VGNVPINYY109 pKa = 8.73 NRR111 pKa = 11.84 LGKK114 pKa = 7.85 VTRR117 pKa = 11.84 IGSVFIGYY125 pKa = 8.87 DD126 pKa = 3.47 FRR128 pKa = 11.84 QVSQVGGLNVLYY140 pKa = 9.99 NHH142 pKa = 7.27 WGDD145 pKa = 3.14 ARR147 pKa = 11.84 YY148 pKa = 9.69 IGQVKK153 pKa = 9.72 YY154 pKa = 10.94 NNHH157 pKa = 6.0 RR158 pKa = 11.84 FNGNFSVYY166 pKa = 9.03 NTWNTWEE173 pKa = 4.04 YY174 pKa = 11.52 DD175 pKa = 3.54 FNNDD179 pKa = 3.01 FFLRR183 pKa = 11.84 SNFRR187 pKa = 11.84 NDD189 pKa = 3.0 FEE191 pKa = 5.62 SFHH194 pKa = 7.07 EE195 pKa = 4.65 DD196 pKa = 3.94 DD197 pKa = 3.58 NYY199 pKa = 11.38 LYY201 pKa = 10.77 YY202 pKa = 10.39 RR203 pKa = 11.84 SKK205 pKa = 10.98 GVKK208 pKa = 9.27 KK209 pKa = 10.32 GKK211 pKa = 9.39 KK212 pKa = 9.34 GNVIKK217 pKa = 10.67 RR218 pKa = 11.84 KK219 pKa = 9.54 KK220 pKa = 9.87 NKK222 pKa = 9.89 QKK224 pKa = 10.45 VFPNKK229 pKa = 7.86 NTRR232 pKa = 11.84 RR233 pKa = 3.75
Molecular weight: 27.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.303
IPC2_protein 9.999
IPC_protein 10.657
Toseland 10.599
ProMoST 10.365
Dawson 10.745
Bjellqvist 10.452
Wikipedia 10.95
Rodwell 11.052
Grimsley 10.818
Solomon 10.804
Lehninger 10.774
Nozaki 10.555
DTASelect 10.452
Thurlkill 10.613
EMBOSS 10.994
Sillero 10.657
Patrickios 10.716
IPC_peptide 10.804
IPC2_peptide 9.238
IPC2.peptide.svr19 8.445
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2946
0
2946
1027031
49
6637
348.6
39.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.46 ± 0.047
0.716 ± 0.015
5.856 ± 0.091
6.735 ± 0.044
5.265 ± 0.033
6.241 ± 0.047
1.676 ± 0.022
8.741 ± 0.042
8.196 ± 0.088
9.059 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.071 ± 0.027
6.798 ± 0.05
3.209 ± 0.025
3.154 ± 0.028
3.113 ± 0.036
6.721 ± 0.041
5.856 ± 0.09
6.045 ± 0.036
1.011 ± 0.016
4.077 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here