Mungbean yellow mosaic virus
Average proteome isoelectric point is 8.65
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q76WK9|Q76WK9_9GEMI 1L4 protein OS=Mungbean yellow mosaic virus OX=33726 GN=1L4 PE=4 SV=1
MM1 pKa = 7.47 PRR3 pKa = 11.84 LGRR6 pKa = 11.84 FAINAKK12 pKa = 10.31 NYY14 pKa = 8.34 FLTYY18 pKa = 9.31 PRR20 pKa = 11.84 CPLRR24 pKa = 11.84 KK25 pKa = 9.05 EE26 pKa = 4.04 DD27 pKa = 4.55 ALEE30 pKa = 4.05 EE31 pKa = 4.04 LLALSTPVNKK41 pKa = 10.24 KK42 pKa = 9.97 FIRR45 pKa = 11.84 VCRR48 pKa = 11.84 EE49 pKa = 3.42 LHH51 pKa = 6.59 EE52 pKa = 5.63 DD53 pKa = 4.09 GEE55 pKa = 4.56 PHH57 pKa = 6.86 LHH59 pKa = 6.48 VLLQFEE65 pKa = 5.68 GKK67 pKa = 9.58 FQTKK71 pKa = 8.6 NEE73 pKa = 4.04 RR74 pKa = 11.84 FFDD77 pKa = 4.07 LVSSTRR83 pKa = 11.84 SAHH86 pKa = 4.49 YY87 pKa = 10.1 HH88 pKa = 6.27 PNVQAAKK95 pKa = 9.85 SASDD99 pKa = 3.33 VKK101 pKa = 11.21 SYY103 pKa = 10.0 MDD105 pKa = 3.66 KK106 pKa = 11.31 DD107 pKa = 3.77 GDD109 pKa = 4.26 VVDD112 pKa = 4.63 HH113 pKa = 7.31 GSFQVDD119 pKa = 3.0 GRR121 pKa = 11.84 SARR124 pKa = 11.84 GGKK127 pKa = 9.48 QSANDD132 pKa = 3.73 AYY134 pKa = 10.87 AEE136 pKa = 4.09 ALNSGSKK143 pKa = 10.17 LQALNILRR151 pKa = 11.84 EE152 pKa = 4.22 KK153 pKa = 10.72 APKK156 pKa = 10.11 DD157 pKa = 3.66 YY158 pKa = 10.78 ILQFHH163 pKa = 6.82 NLNCNLSRR171 pKa = 11.84 IFSDD175 pKa = 3.71 EE176 pKa = 3.88 VPLYY180 pKa = 10.27 VSPYY184 pKa = 9.88 SLSAFDD190 pKa = 4.97 KK191 pKa = 8.82 VPSYY195 pKa = 10.55 ISSWASEE202 pKa = 4.24 NVRR205 pKa = 11.84 HH206 pKa = 6.07 PAAPEE211 pKa = 3.75 RR212 pKa = 11.84 PISIVIEE219 pKa = 4.23 GDD221 pKa = 3.2 SRR223 pKa = 11.84 TGKK226 pKa = 8.55 TMWARR231 pKa = 11.84 ALGPHH236 pKa = 6.7 NYY238 pKa = 10.19 LCGHH242 pKa = 7.39 LDD244 pKa = 4.09 LNSKK248 pKa = 10.03 IYY250 pKa = 11.22 SNDD253 pKa = 2.14 AWYY256 pKa = 10.92 NVIDD260 pKa = 5.2 DD261 pKa = 4.41 VDD263 pKa = 3.61 PHH265 pKa = 5.85 YY266 pKa = 11.0 LKK268 pKa = 10.7 HH269 pKa = 6.2 FKK271 pKa = 10.7 EE272 pKa = 4.46 FMGAQRR278 pKa = 11.84 DD279 pKa = 3.63 WQSNVKK285 pKa = 9.78 YY286 pKa = 10.47 GKK288 pKa = 7.21 PTHH291 pKa = 6.52 IKK293 pKa = 10.55 GGIPTIFLCNPGPKK307 pKa = 9.27 SSYY310 pKa = 10.57 KK311 pKa = 10.43 EE312 pKa = 4.06 YY313 pKa = 10.85 LDD315 pKa = 4.39 EE316 pKa = 6.14 ADD318 pKa = 3.59 NTALKK323 pKa = 10.54 LWASKK328 pKa = 10.37 NAEE331 pKa = 4.13 FYY333 pKa = 9.64 TLKK336 pKa = 10.62 EE337 pKa = 4.03 PLFSSVDD344 pKa = 3.38 QSATQGCQEE353 pKa = 4.6 ASNSTLSNN361 pKa = 3.4
Molecular weight: 40.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.583
IPC2_protein 6.634
IPC_protein 6.781
Toseland 6.868
ProMoST 7.176
Dawson 7.278
Bjellqvist 7.205
Wikipedia 7.263
Rodwell 7.278
Grimsley 6.985
Solomon 7.322
Lehninger 7.337
Nozaki 7.527
DTASelect 7.571
Thurlkill 7.6
EMBOSS 7.629
Sillero 7.688
Patrickios 4.164
IPC_peptide 7.322
IPC2_peptide 7.102
IPC2.peptide.svr19 7.038
Protein with the highest isoelectric point:
>tr|Q76WL1|Q76WL1_9GEMI Protein V2 OS=Mungbean yellow mosaic virus OX=33726 GN=1R2 PE=3 SV=1
MM1 pKa = 7.18 SNVRR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 LIFDD11 pKa = 3.79 TPLSLPATAGSVPASAKK28 pKa = 9.31 RR29 pKa = 11.84 RR30 pKa = 11.84 RR31 pKa = 11.84 WTNRR35 pKa = 11.84 PMWRR39 pKa = 11.84 KK40 pKa = 7.82 PRR42 pKa = 11.84 YY43 pKa = 7.63 YY44 pKa = 10.29 RR45 pKa = 11.84 LYY47 pKa = 10.24 RR48 pKa = 11.84 SPDD51 pKa = 3.2 VPRR54 pKa = 11.84 GCEE57 pKa = 4.34 GPCKK61 pKa = 10.07 VQSFEE66 pKa = 4.11 AKK68 pKa = 10.01 HH69 pKa = 6.45 DD70 pKa = 3.59 ISHH73 pKa = 7.09 LGKK76 pKa = 10.4 VICVTDD82 pKa = 3.33 VTRR85 pKa = 11.84 GNGITHH91 pKa = 6.78 RR92 pKa = 11.84 VGKK95 pKa = 9.5 RR96 pKa = 11.84 FCVKK100 pKa = 10.5 SIWVTGKK107 pKa = 9.22 IWMDD111 pKa = 3.31 EE112 pKa = 4.07 NIKK115 pKa = 9.32 TKK117 pKa = 10.61 NHH119 pKa = 6.01 TNTVMFKK126 pKa = 10.36 LVRR129 pKa = 11.84 DD130 pKa = 3.79 RR131 pKa = 11.84 RR132 pKa = 11.84 PFGTPQDD139 pKa = 3.81 FGQVFNMYY147 pKa = 10.66 DD148 pKa = 3.86 NEE150 pKa = 4.34 PSTATVKK157 pKa = 10.81 NDD159 pKa = 2.84 LRR161 pKa = 11.84 DD162 pKa = 3.4 RR163 pKa = 11.84 YY164 pKa = 9.76 QVVRR168 pKa = 11.84 KK169 pKa = 8.4 FQATVTGGQYY179 pKa = 10.83 ASKK182 pKa = 9.91 EE183 pKa = 3.76 QAIVSKK189 pKa = 9.71 FYY191 pKa = 10.67 RR192 pKa = 11.84 VNNYY196 pKa = 7.79 VVYY199 pKa = 10.46 NHH201 pKa = 6.03 QEE203 pKa = 3.42 AAKK206 pKa = 10.4 YY207 pKa = 9.25 EE208 pKa = 4.13 NHH210 pKa = 6.46 TEE212 pKa = 3.99 NALLLYY218 pKa = 7.29 MACTHH223 pKa = 7.07 ASNPVYY229 pKa = 9.86 ATLKK233 pKa = 9.47 IRR235 pKa = 11.84 IYY237 pKa = 10.59 FYY239 pKa = 11.32 DD240 pKa = 4.06 SISNN244 pKa = 3.66
Molecular weight: 28.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.268
IPC2_protein 9.531
IPC_protein 9.736
Toseland 10.028
ProMoST 9.794
Dawson 10.262
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.584
Grimsley 10.35
Solomon 10.292
Lehninger 10.248
Nozaki 10.072
DTASelect 9.97
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.189
Patrickios 9.911
IPC_peptide 10.292
IPC2_peptide 8.873
IPC2.peptide.svr19 8.487
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
1249
74
361
178.4
20.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.444 ± 0.904
1.761 ± 0.229
4.564 ± 0.708
3.843 ± 0.598
4.323 ± 0.219
5.124 ± 0.427
3.683 ± 0.463
5.685 ± 0.981
6.725 ± 0.327
9.367 ± 1.339
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.562 ± 0.623
5.925 ± 0.397
4.804 ± 0.653
3.283 ± 0.448
6.325 ± 0.822
8.487 ± 0.954
5.765 ± 1.15
6.165 ± 0.801
1.441 ± 0.125
4.724 ± 0.516
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here