Vibrio phage vB_VspS_VS-ABTNL-3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D0ZP74|A0A2D0ZP74_9CAUD Uncharacterized protein OS=Vibrio phage vB_VspS_VS-ABTNL-3 OX=2026084 GN=PVS_38 PE=4 SV=1
MM1 pKa = 8.05IGDD4 pKa = 4.62LDD6 pKa = 4.17GTFCDD11 pKa = 3.54NSHH14 pKa = 6.4RR15 pKa = 11.84QNLLPTGEE23 pKa = 4.4GTTTADD29 pKa = 2.5WVAFNGACRR38 pKa = 11.84DD39 pKa = 3.97DD40 pKa = 4.31EE41 pKa = 5.23PIAVPLGMWCEE52 pKa = 4.65FYY54 pKa = 10.07TACDD58 pKa = 3.42EE59 pKa = 5.35AILLTGRR66 pKa = 11.84GSSCAPQTKK75 pKa = 9.28EE76 pKa = 3.33WLGEE80 pKa = 3.83YY81 pKa = 9.82GVPYY85 pKa = 10.23DD86 pKa = 4.17EE87 pKa = 6.88LIMRR91 pKa = 11.84PEE93 pKa = 4.37GDD95 pKa = 3.16NRR97 pKa = 11.84KK98 pKa = 8.61ATDD101 pKa = 3.99FKK103 pKa = 11.15RR104 pKa = 11.84EE105 pKa = 3.78QFEE108 pKa = 4.33RR109 pKa = 11.84LGLTRR114 pKa = 11.84DD115 pKa = 4.17DD116 pKa = 5.06IVFEE120 pKa = 5.06DD121 pKa = 4.77DD122 pKa = 3.54PAVIAMLRR130 pKa = 11.84NDD132 pKa = 4.1FEE134 pKa = 5.64CIVVAVPSGCSAVQSGVSNNGDD156 pKa = 3.32GTRR159 pKa = 11.84FDD161 pKa = 4.43SDD163 pKa = 3.78VKK165 pKa = 11.17VADD168 pKa = 3.96TNTSYY173 pKa = 11.18EE174 pKa = 4.11GG175 pKa = 3.34

Molecular weight: 19.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1A5Y9|A0A2D1A5Y9_9CAUD Putative DNA helicase OS=Vibrio phage vB_VspS_VS-ABTNL-3 OX=2026084 GN=PVS_35 PE=4 SV=1
MM1 pKa = 7.48KK2 pKa = 10.28NSKK5 pKa = 10.66GYY7 pKa = 10.14DD8 pKa = 3.19FKK10 pKa = 11.24KK11 pKa = 8.51IHH13 pKa = 5.48EE14 pKa = 4.34RR15 pKa = 11.84NRR17 pKa = 11.84RR18 pKa = 11.84EE19 pKa = 3.78AEE21 pKa = 3.68KK22 pKa = 10.01RR23 pKa = 11.84RR24 pKa = 11.84EE25 pKa = 3.99RR26 pKa = 11.84IVVGMGITFSAGCVVFALMFLLLRR50 pKa = 11.84LAQVLGIEE58 pKa = 4.58FF59 pKa = 4.07

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

13146

59

1790

257.8

28.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.581 ± 0.6

0.974 ± 0.176

6.283 ± 0.223

7.592 ± 0.291

3.826 ± 0.254

7.379 ± 0.271

1.651 ± 0.138

5.097 ± 0.283

6.641 ± 0.226

7.957 ± 0.233

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.332 ± 0.212

4.511 ± 0.185

4.161 ± 0.257

4.062 ± 0.271

4.671 ± 0.232

5.918 ± 0.299

6.025 ± 0.271

6.428 ± 0.314

1.575 ± 0.14

3.339 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski