Medicago truncatula (Barrel medic) (Medicago tribuloides)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; NPAAA clade; Hologalegina;

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 57067 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G7LHX6|G7LHX6_MEDTR Putative transcription factor C2H2 family OS=Medicago truncatula OX=3880 GN=MTR_8g085530 PE=4 SV=2
MM1 pKa = 7.94DD2 pKa = 6.14PYY4 pKa = 11.39FNLYY8 pKa = 10.66DD9 pKa = 3.58IFQKK13 pKa = 10.71VSTRR17 pKa = 11.84ILDD20 pKa = 3.64TVLYY24 pKa = 9.71CIRR27 pKa = 11.84DD28 pKa = 3.7CAKK31 pKa = 10.69EE32 pKa = 3.88MVALNVEE39 pKa = 4.37GRR41 pKa = 11.84DD42 pKa = 3.49ILDD45 pKa = 3.51MKK47 pKa = 11.06VSLHH51 pKa = 5.07VNPYY55 pKa = 9.73IVEE58 pKa = 4.64DD59 pKa = 4.02YY60 pKa = 10.76PFEE63 pKa = 3.97QSHH66 pKa = 6.78RR67 pKa = 11.84NLHH70 pKa = 5.68DD71 pKa = 3.4QQNEE75 pKa = 3.88DD76 pKa = 4.16SLDD79 pKa = 3.72EE80 pKa = 4.76LEE82 pKa = 5.89GDD84 pKa = 4.12DD85 pKa = 5.3LLHH88 pKa = 6.63EE89 pKa = 4.56LEE91 pKa = 4.87EE92 pKa = 5.87DD93 pKa = 3.88DD94 pKa = 6.59FLLEE98 pKa = 3.95LQEE101 pKa = 5.28DD102 pKa = 3.99HH103 pKa = 7.54FEE105 pKa = 4.46QNPHH109 pKa = 6.37NYY111 pKa = 9.71NDD113 pKa = 3.68QQSLDD118 pKa = 3.67FSDD121 pKa = 3.25QLEE124 pKa = 4.47YY125 pKa = 11.33NDD127 pKa = 5.74DD128 pKa = 3.86LLEE131 pKa = 4.28EE132 pKa = 4.7LGEE135 pKa = 4.01EE136 pKa = 4.79DD137 pKa = 5.55RR138 pKa = 11.84FEE140 pKa = 4.28QYY142 pKa = 10.56LRR144 pKa = 11.84NYY146 pKa = 10.45NDD148 pKa = 3.2QQTVDD153 pKa = 4.12LLEE156 pKa = 4.34EE157 pKa = 4.69LEE159 pKa = 5.37DD160 pKa = 5.28DD161 pKa = 5.28DD162 pKa = 6.0FLHH165 pKa = 6.16EE166 pKa = 4.51QYY168 pKa = 10.97PHH170 pKa = 7.46IYY172 pKa = 10.33NDD174 pKa = 3.49QQNVDD179 pKa = 4.9LLDD182 pKa = 3.49QLEE185 pKa = 4.7DD186 pKa = 3.82DD187 pKa = 4.89DD188 pKa = 6.57FVEE191 pKa = 4.62EE192 pKa = 4.31WEE194 pKa = 4.62EE195 pKa = 4.11DD196 pKa = 3.72DD197 pKa = 5.09SLHH200 pKa = 6.28EE201 pKa = 4.17LEE203 pKa = 4.99EE204 pKa = 4.21DD205 pKa = 3.6HH206 pKa = 7.54FEE208 pKa = 4.87HH209 pKa = 7.44NYY211 pKa = 10.57NDD213 pKa = 3.71QQNVDD218 pKa = 4.9LLDD221 pKa = 3.54QLEE224 pKa = 4.68DD225 pKa = 4.15DD226 pKa = 4.67NLLQEE231 pKa = 4.89LEE233 pKa = 4.16EE234 pKa = 4.37DD235 pKa = 3.73HH236 pKa = 7.15FGQYY240 pKa = 7.87PHH242 pKa = 7.38NYY244 pKa = 8.58IDD246 pKa = 3.71QQNMDD251 pKa = 5.01LLDD254 pKa = 3.59QLEE257 pKa = 4.61DD258 pKa = 4.15DD259 pKa = 5.18DD260 pKa = 5.83LLEE263 pKa = 4.42EE264 pKa = 4.89LEE266 pKa = 4.95EE267 pKa = 6.15DD268 pKa = 5.48DD269 pKa = 6.37SLQNWKK275 pKa = 10.43KK276 pKa = 10.27IILSIIIMINKK287 pKa = 9.1IWICNINNSSFQSLLKK303 pKa = 10.54LYY305 pKa = 10.38VSRR308 pKa = 11.84IMM310 pKa = 4.86

Molecular weight: 37.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G7J4N2|G7J4N2_MEDTR Uncharacterized protein OS=Medicago truncatula OX=3880 GN=MTR_3g113040 PE=4 SV=1
MM1 pKa = 7.9SIPNIGSKK9 pKa = 10.01KK10 pKa = 9.92KK11 pKa = 10.39KK12 pKa = 10.25NNNQVVWAPVARR24 pKa = 11.84SPFQGRR30 pKa = 11.84IRR32 pKa = 11.84GIPGSISRR40 pKa = 11.84GNNTWPVHH48 pKa = 4.93MPLRR52 pKa = 11.84MSRR55 pKa = 11.84ISRR58 pKa = 11.84SPLMGRR64 pKa = 11.84KK65 pKa = 8.6PVRR68 pKa = 11.84KK69 pKa = 9.89LKK71 pKa = 10.8

Molecular weight: 8.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49956

7111

57067

19735905

16

5385

345.8

38.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.81 ± 0.009

1.951 ± 0.005

5.298 ± 0.007

6.305 ± 0.012

4.508 ± 0.008

6.105 ± 0.01

2.498 ± 0.005

5.893 ± 0.009

6.526 ± 0.01

9.841 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.005

5.098 ± 0.008

4.607 ± 0.011

3.623 ± 0.008

4.84 ± 0.008

8.857 ± 0.011

5.062 ± 0.007

6.498 ± 0.007

1.282 ± 0.004

2.934 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski