Amino acid dipepetide frequency for Medicago truncatula (Barrel medic) (Medicago tribuloides)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.854AlaAla: 4.854 ± 0.025	1.107AlaCys: 1.107 ± 0.007	2.603AlaAsp: 2.603 ± 0.013	3.524AlaGlu: 3.524 ± 0.015	2.564AlaPhe: 2.564 ± 0.011	3.328AlaGly: 3.328 ± 0.017	1.176AlaHis: 1.176 ± 0.006	3.595AlaIle: 3.595 ± 0.014	3.635AlaLys: 3.635 ± 0.015	5.787AlaLeu: 5.787 ± 0.023	1.567AlaMet: 1.567 ± 0.01	2.506AlaAsn: 2.506 ± 0.012	2.333AlaPro: 2.333 ± 0.015	1.845AlaGln: 1.845 ± 0.011	2.789AlaArg: 2.789 ± 0.012	5.223AlaSer: 5.223 ± 0.018	3.292AlaThr: 3.292 ± 0.013	4.014AlaVal: 4.014 ± 0.018	0.68AlaTrp: 0.68 ± 0.006	1.673AlaTyr: 1.673 ± 0.009	0.0AlaXaa: 0.0 ± 0.0
Cys	0.906CysAla: 0.906 ± 0.007	0.585CysCys: 0.585 ± 0.007	0.937CysAsp: 0.937 ± 0.007	0.944CysGlu: 0.944 ± 0.007	1.044CysPhe: 1.044 ± 0.007	1.366CysGly: 1.366 ± 0.009	0.526CysHis: 0.526 ± 0.005	1.167CysIle: 1.167 ± 0.008	1.262CysLys: 1.262 ± 0.008	1.966CysLeu: 1.966 ± 0.012	0.474CysMet: 0.474 ± 0.005	1.017CysAsn: 1.017 ± 0.008	0.96CysPro: 0.96 ± 0.008	0.602CysGln: 0.602 ± 0.005	0.98CysArg: 0.98 ± 0.008	1.817CysSer: 1.817 ± 0.011	0.888CysThr: 0.888 ± 0.007	1.19CysVal: 1.19 ± 0.008	0.263CysTrp: 0.263 ± 0.004	0.617CysTyr: 0.617 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	3.114AspAla: 3.114 ± 0.013	1.049AspCys: 1.049 ± 0.008	3.795AspAsp: 3.795 ± 0.02	3.953AspGlu: 3.953 ± 0.017	2.518AspPhe: 2.518 ± 0.012	3.541AspGly: 3.541 ± 0.015	1.245AspHis: 1.245 ± 0.008	3.33AspIle: 3.33 ± 0.015	2.914AspLys: 2.914 ± 0.012	5.065AspLeu: 5.065 ± 0.018	1.324AspMet: 1.324 ± 0.008	2.403AspAsn: 2.403 ± 0.013	2.335AspPro: 2.335 ± 0.013	1.723AspGln: 1.723 ± 0.009	2.167AspArg: 2.167 ± 0.012	4.192AspSer: 4.192 ± 0.018	2.281AspThr: 2.281 ± 0.012	3.733AspVal: 3.733 ± 0.013	0.698AspTrp: 0.698 ± 0.006	1.598AspTyr: 1.598 ± 0.01	0.001AspXaa: 0.001 ± 0.0
Glu	4.042GluAla: 4.042 ± 0.017	0.977GluCys: 0.977 ± 0.008	3.834GluAsp: 3.834 ± 0.016	5.832GluGlu: 5.832 ± 0.038	2.46GluPhe: 2.46 ± 0.01	3.484GluGly: 3.484 ± 0.014	1.302GluHis: 1.302 ± 0.008	4.058GluIle: 4.058 ± 0.015	4.918GluLys: 4.918 ± 0.02	5.807GluLeu: 5.807 ± 0.021	1.678GluMet: 1.678 ± 0.011	3.504GluAsn: 3.504 ± 0.014	1.913GluPro: 1.913 ± 0.012	2.04GluGln: 2.04 ± 0.01	3.065GluArg: 3.065 ± 0.016	4.544GluSer: 4.544 ± 0.018	3.031GluThr: 3.031 ± 0.014	4.124GluVal: 4.124 ± 0.016	0.728GluTrp: 0.728 ± 0.007	1.712GluTyr: 1.712 ± 0.01	0.0GluXaa: 0.0 ± 0.0
Phe	2.343PheAla: 2.343 ± 0.012	1.022PheCys: 1.022 ± 0.008	2.529PheAsp: 2.529 ± 0.011	2.489PheGlu: 2.489 ± 0.011	2.163PhePhe: 2.163 ± 0.012	3.162PheGly: 3.162 ± 0.014	1.259PheHis: 1.259 ± 0.008	2.493PheIle: 2.493 ± 0.012	2.47PheLys: 2.47 ± 0.013	4.578PheLeu: 4.578 ± 0.017	1.067PheMet: 1.067 ± 0.007	2.171PheAsn: 2.171 ± 0.011	2.245PhePro: 2.245 ± 0.011	1.69PheGln: 1.69 ± 0.01	1.966PheArg: 1.966 ± 0.01	4.257PheSer: 4.257 ± 0.016	2.218PheThr: 2.218 ± 0.011	2.974PheVal: 2.974 ± 0.012	0.594PheTrp: 0.594 ± 0.006	1.391PheTyr: 1.391 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	3.213GlyAla: 3.213 ± 0.016	1.309GlyCys: 1.309 ± 0.009	3.177GlyAsp: 3.177 ± 0.013	3.42GlyGlu: 3.42 ± 0.015	3.11GlyPhe: 3.11 ± 0.014	4.863GlyGly: 4.863 ± 0.037	1.403GlyHis: 1.403 ± 0.009	3.691GlyIle: 3.691 ± 0.017	4.051GlyLys: 4.051 ± 0.014	5.512GlyLeu: 5.512 ± 0.018	1.476GlyMet: 1.476 ± 0.01	3.197GlyAsn: 3.197 ± 0.016	2.093GlyPro: 2.093 ± 0.012	1.861GlyGln: 1.861 ± 0.01	3.162GlyArg: 3.162 ± 0.016	5.454GlySer: 5.454 ± 0.019	3.092GlyThr: 3.092 ± 0.014	4.024GlyVal: 4.024 ± 0.016	0.865GlyTrp: 0.865 ± 0.007	2.077GlyTyr: 2.077 ± 0.012	0.0GlyXaa: 0.0 ± 0.0
His	1.365HisAla: 1.365 ± 0.008	0.55HisCys: 0.55 ± 0.006	1.289HisAsp: 1.289 ± 0.008	1.347HisGlu: 1.347 ± 0.008	1.196HisPhe: 1.196 ± 0.008	1.652HisGly: 1.652 ± 0.01	1.021HisHis: 1.021 ± 0.011	1.407HisIle: 1.407 ± 0.009	1.322HisLys: 1.322 ± 0.01	2.603HisLeu: 2.603 ± 0.012	0.587HisMet: 0.587 ± 0.006	1.22HisAsn: 1.22 ± 0.008	1.268HisPro: 1.268 ± 0.008	1.046HisGln: 1.046 ± 0.007	1.289HisArg: 1.289 ± 0.008	2.015HisSer: 2.015 ± 0.011	1.085HisThr: 1.085 ± 0.008	1.682HisVal: 1.682 ± 0.009	0.298HisTrp: 0.298 ± 0.004	0.738HisTyr: 0.738 ± 0.006	0.0HisXaa: 0.0 ± 0.0
Ile	3.571IleAla: 3.571 ± 0.016	1.235IleCys: 1.235 ± 0.01	3.234IleAsp: 3.234 ± 0.014	3.472IleGlu: 3.472 ± 0.015	2.68IlePhe: 2.68 ± 0.014	3.711IleGly: 3.711 ± 0.016	1.535IleHis: 1.535 ± 0.009	3.484IleIle: 3.484 ± 0.016	3.405IleLys: 3.405 ± 0.015	5.834IleLeu: 5.834 ± 0.02	1.319IleMet: 1.319 ± 0.009	2.751IleAsn: 2.751 ± 0.014	3.25IlePro: 3.25 ± 0.018	2.182IleGln: 2.182 ± 0.011	2.626IleArg: 2.626 ± 0.011	5.326IleSer: 5.326 ± 0.017	2.983IleThr: 2.983 ± 0.013	3.827IleVal: 3.827 ± 0.015	0.791IleTrp: 0.791 ± 0.007	1.715IleTyr: 1.715 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.661LysAla: 3.661 ± 0.015	1.102LysCys: 1.102 ± 0.009	3.467LysAsp: 3.467 ± 0.016	4.874LysGlu: 4.874 ± 0.022	2.514LysPhe: 2.514 ± 0.011	3.761LysGly: 3.761 ± 0.014	1.552LysHis: 1.552 ± 0.01	3.903LysIle: 3.903 ± 0.015	5.251LysLys: 5.251 ± 0.03	6.524LysLeu: 6.524 ± 0.027	1.656LysMet: 1.656 ± 0.009	3.335LysAsn: 3.335 ± 0.015	2.662LysPro: 2.662 ± 0.015	2.356LysGln: 2.356 ± 0.012	3.529LysArg: 3.529 ± 0.015	5.014LysSer: 5.014 ± 0.02	3.225LysThr: 3.225 ± 0.012	4.182LysVal: 4.182 ± 0.017	0.858LysTrp: 0.858 ± 0.007	1.831LysTyr: 1.831 ± 0.01	0.0LysXaa: 0.0 ± 0.0
Leu	5.699LeuAla: 5.699 ± 0.021	1.999LeuCys: 1.999 ± 0.011	5.154LeuAsp: 5.154 ± 0.018	6.359LeuGlu: 6.359 ± 0.026	4.221LeuPhe: 4.221 ± 0.018	5.402LeuGly: 5.402 ± 0.02	2.724LeuHis: 2.724 ± 0.013	5.225LeuIle: 5.225 ± 0.02	6.822LeuLys: 6.822 ± 0.026	9.819LeuLeu: 9.819 ± 0.033	2.159LeuMet: 2.159 ± 0.009	4.606LeuAsn: 4.606 ± 0.017	5.096LeuPro: 5.096 ± 0.018	4.277LeuGln: 4.277 ± 0.018	5.075LeuArg: 5.075 ± 0.019	8.853LeuSer: 8.853 ± 0.034	4.745LeuThr: 4.745 ± 0.018	6.348LeuVal: 6.348 ± 0.018	1.181LeuTrp: 1.181 ± 0.008	2.65LeuTyr: 2.65 ± 0.013	0.001LeuXaa: 0.001 ± 0.0
Met	1.844MetAla: 1.844 ± 0.01	0.383MetCys: 0.383 ± 0.004	1.411MetAsp: 1.411 ± 0.008	1.89MetGlu: 1.89 ± 0.011	0.954MetPhe: 0.954 ± 0.007	1.452MetGly: 1.452 ± 0.009	0.591MetHis: 0.591 ± 0.006	1.359MetIle: 1.359 ± 0.01	1.849MetLys: 1.849 ± 0.01	2.278MetLeu: 2.278 ± 0.01	0.73MetMet: 0.73 ± 0.007	1.234MetAsn: 1.234 ± 0.008	0.98MetPro: 0.98 ± 0.009	0.918MetGln: 0.918 ± 0.008	1.123MetArg: 1.123 ± 0.008	1.881MetSer: 1.881 ± 0.01	1.164MetThr: 1.164 ± 0.008	1.687MetVal: 1.687 ± 0.012	0.282MetTrp: 0.282 ± 0.004	0.614MetTyr: 0.614 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.609AsnAla: 2.609 ± 0.012	1.025AsnCys: 1.025 ± 0.01	2.561AsnAsp: 2.561 ± 0.011	2.896AsnGlu: 2.896 ± 0.012	2.344AsnPhe: 2.344 ± 0.013	3.411AsnGly: 3.411 ± 0.015	1.371AsnHis: 1.371 ± 0.009	3.208AsnIle: 3.208 ± 0.016	3.0AsnLys: 3.0 ± 0.013	5.438AsnLeu: 5.438 ± 0.026	1.362AsnMet: 1.362 ± 0.009	3.241AsnAsn: 3.241 ± 0.021	2.382AsnPro: 2.382 ± 0.015	1.952AsnGln: 1.952 ± 0.013	2.032AsnArg: 2.032 ± 0.012	4.366AsnSer: 4.366 ± 0.017	2.324AsnThr: 2.324 ± 0.014	3.261AsnVal: 3.261 ± 0.015	0.663AsnTrp: 0.663 ± 0.006	1.537AsnTyr: 1.537 ± 0.009	0.0AsnXaa: 0.0 ± 0.0
Pro	2.338ProAla: 2.338 ± 0.014	0.766ProCys: 0.766 ± 0.006	2.186ProAsp: 2.186 ± 0.011	2.69ProGlu: 2.69 ± 0.016	2.112ProPhe: 2.112 ± 0.012	2.207ProGly: 2.207 ± 0.014	1.115ProHis: 1.115 ± 0.007	2.54ProIle: 2.54 ± 0.011	2.888ProLys: 2.888 ± 0.014	4.221ProLeu: 4.221 ± 0.017	0.975ProMet: 0.975 ± 0.007	2.592ProAsn: 2.592 ± 0.014	3.495ProPro: 3.495 ± 0.042	1.743ProGln: 1.743 ± 0.012	2.05ProArg: 2.05 ± 0.011	4.919ProSer: 4.919 ± 0.024	2.631ProThr: 2.631 ± 0.016	2.73ProVal: 2.73 ± 0.016	0.54ProTrp: 0.54 ± 0.005	1.33ProTyr: 1.33 ± 0.01	0.0ProXaa: 0.0 ± 0.0
Gln	1.995GlnAla: 1.995 ± 0.015	0.602GlnCys: 0.602 ± 0.005	1.621GlnAsp: 1.621 ± 0.009	2.31GlnGlu: 2.31 ± 0.012	1.503GlnPhe: 1.503 ± 0.01	1.965GlnGly: 1.965 ± 0.011	0.993GlnHis: 0.993 ± 0.009	2.187GlnIle: 2.187 ± 0.011	2.405GlnLys: 2.405 ± 0.014	3.621GlnLeu: 3.621 ± 0.017	0.962GlnMet: 0.962 ± 0.007	2.092GlnAsn: 2.092 ± 0.011	1.655GlnPro: 1.655 ± 0.011	2.02GlnGln: 2.02 ± 0.024	1.938GlnArg: 1.938 ± 0.011	2.805GlnSer: 2.805 ± 0.014	1.839GlnThr: 1.839 ± 0.011	2.296GlnVal: 2.296 ± 0.009	0.438GlnTrp: 0.438 ± 0.006	0.979GlnTyr: 0.979 ± 0.008	0.0GlnXaa: 0.0 ± 0.0
Arg	2.595ArgAla: 2.595 ± 0.014	0.945ArgCys: 0.945 ± 0.007	2.471ArgAsp: 2.471 ± 0.011	3.016ArgGlu: 3.016 ± 0.015	2.151ArgPhe: 2.151 ± 0.01	2.798ArgGly: 2.798 ± 0.015	1.226ArgHis: 1.226 ± 0.008	2.837ArgIle: 2.837 ± 0.013	3.735ArgLys: 3.735 ± 0.018	4.628ArgLeu: 4.628 ± 0.017	1.242ArgMet: 1.242 ± 0.008	2.562ArgAsn: 2.562 ± 0.013	1.935ArgPro: 1.935 ± 0.012	1.612ArgGln: 1.612 ± 0.01	3.281ArgArg: 3.281 ± 0.018	3.866ArgSer: 3.866 ± 0.019	2.286ArgThr: 2.286 ± 0.012	3.098ArgVal: 3.098 ± 0.014	0.679ArgTrp: 0.679 ± 0.006	1.441ArgTyr: 1.441 ± 0.008	0.0ArgXaa: 0.0 ± 0.0
Ser	4.43SerAla: 4.43 ± 0.019	1.748SerCys: 1.748 ± 0.009	4.358SerAsp: 4.358 ± 0.016	4.596SerGlu: 4.596 ± 0.016	4.358SerPhe: 4.358 ± 0.017	5.249SerGly: 5.249 ± 0.024	2.161SerHis: 2.161 ± 0.012	5.121SerIle: 5.121 ± 0.018	5.286SerLys: 5.286 ± 0.02	9.166SerLeu: 9.166 ± 0.032	2.098SerMet: 2.098 ± 0.01	4.676SerAsn: 4.676 ± 0.023	4.118SerPro: 4.118 ± 0.027	3.032SerGln: 3.032 ± 0.014	4.003SerArg: 4.003 ± 0.019	10.601SerSer: 10.601 ± 0.04	4.827SerThr: 4.827 ± 0.019	5.038SerVal: 5.038 ± 0.016	1.169SerTrp: 1.169 ± 0.007	2.534SerTyr: 2.534 ± 0.012	0.0SerXaa: 0.0 ± 0.0
Thr	2.833ThrAla: 2.833 ± 0.013	0.977ThrCys: 0.977 ± 0.007	2.231ThrAsp: 2.231 ± 0.012	2.676ThrGlu: 2.676 ± 0.014	2.291ThrPhe: 2.291 ± 0.012	2.929ThrGly: 2.929 ± 0.016	1.19ThrHis: 1.19 ± 0.009	3.256ThrIle: 3.256 ± 0.015	3.135ThrLys: 3.135 ± 0.013	5.119ThrLeu: 5.119 ± 0.017	1.262ThrMet: 1.262 ± 0.008	2.626ThrAsn: 2.626 ± 0.015	2.557ThrPro: 2.557 ± 0.016	1.651ThrGln: 1.651 ± 0.01	2.319ThrArg: 2.319 ± 0.012	4.815ThrSer: 4.815 ± 0.019	3.433ThrThr: 3.433 ± 0.017	3.181ThrVal: 3.181 ± 0.017	0.649ThrTrp: 0.649 ± 0.005	1.485ThrTyr: 1.485 ± 0.01	0.0ThrXaa: 0.0 ± 0.0
Val	4.324ValAla: 4.324 ± 0.017	1.192ValCys: 1.192 ± 0.009	3.785ValAsp: 3.785 ± 0.015	4.389ValGlu: 4.389 ± 0.019	2.869ValPhe: 2.869 ± 0.012	3.947ValGly: 3.947 ± 0.016	1.518ValHis: 1.518 ± 0.009	3.617ValIle: 3.617 ± 0.016	4.119ValLys: 4.119 ± 0.017	6.223ValLeu: 6.223 ± 0.019	1.556ValMet: 1.556 ± 0.009	2.891ValAsn: 2.891 ± 0.014	3.041ValPro: 3.041 ± 0.015	2.252ValGln: 2.252 ± 0.012	2.864ValArg: 2.864 ± 0.013	5.311ValSer: 5.311 ± 0.017	3.298ValThr: 3.298 ± 0.014	4.975ValVal: 4.975 ± 0.021	0.801ValTrp: 0.801 ± 0.007	2.01ValTyr: 2.01 ± 0.011	0.001ValXaa: 0.001 ± 0.0
Trp	0.63TrpAla: 0.63 ± 0.007	0.269TrpCys: 0.269 ± 0.004	0.654TrpAsp: 0.654 ± 0.006	0.715TrpGlu: 0.715 ± 0.005	0.618TrpPhe: 0.618 ± 0.006	0.685TrpGly: 0.685 ± 0.006	0.309TrpHis: 0.309 ± 0.004	0.802TrpIle: 0.802 ± 0.007	1.055TrpLys: 1.055 ± 0.007	1.277TrpLeu: 1.277 ± 0.009	0.342TrpMet: 0.342 ± 0.004	0.797TrpAsn: 0.797 ± 0.007	0.459TrpPro: 0.459 ± 0.005	0.415TrpGln: 0.415 ± 0.005	0.785TrpArg: 0.785 ± 0.006	0.996TrpSer: 0.996 ± 0.008	0.631TrpThr: 0.631 ± 0.006	0.773TrpVal: 0.773 ± 0.007	0.246TrpTrp: 0.246 ± 0.004	0.367TrpTyr: 0.367 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.73TyrAla: 1.73 ± 0.01	0.671TyrCys: 0.671 ± 0.006	1.682TyrAsp: 1.682 ± 0.009	1.659TyrGlu: 1.659 ± 0.01	1.412TyrPhe: 1.412 ± 0.009	2.138TyrGly: 2.138 ± 0.013	0.76TyrHis: 0.76 ± 0.006	1.652TyrIle: 1.652 ± 0.01	1.736TyrLys: 1.736 ± 0.012	2.946TyrLeu: 2.946 ± 0.014	0.788TyrMet: 0.788 ± 0.007	1.558TyrAsn: 1.558 ± 0.01	1.292TyrPro: 1.292 ± 0.009	1.009TyrGln: 1.009 ± 0.008	1.362TyrArg: 1.362 ± 0.009	2.317TyrSer: 2.317 ± 0.011	1.341TyrThr: 1.341 ± 0.01	1.847TyrVal: 1.847 ± 0.011	0.399TyrTrp: 0.399 ± 0.005	1.044TyrTyr: 1.044 ± 0.01	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 57067 proteins (19735905 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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