Ornithobacterium rhinotracheale (strain ATCC 51463 / DSM 15997 / CCUG 23171 / CIP 104009 / LMG 9086)
Average proteome isoelectric point is 6.8
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2138 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I3ZZL8|I3ZZL8_ORNRL Isoleucine patch superfamily enzyme carbonic anhydrase/acetyltransferase OS=Ornithobacterium rhinotracheale (strain ATCC 51463 / DSM 15997 / CCUG 23171 / CIP 104009 / LMG 9086) OX=867902 GN=Ornrh_0959 PE=4 SV=1
MM1 pKa = 7.27 IQFFSEE7 pKa = 4.52 TEE9 pKa = 3.99 DD10 pKa = 3.47 FTLEE14 pKa = 4.05 DD15 pKa = 3.7 SQVFADD21 pKa = 4.11 WLNACAEE28 pKa = 4.05 RR29 pKa = 11.84 HH30 pKa = 5.59 GYY32 pKa = 9.45 EE33 pKa = 4.37 IEE35 pKa = 4.89 DD36 pKa = 3.42 INYY39 pKa = 8.99 IFCDD43 pKa = 3.85 DD44 pKa = 4.51 EE45 pKa = 4.43 YY46 pKa = 11.89 LLAINQKK53 pKa = 10.11 HH54 pKa = 6.56 LDD56 pKa = 3.34 HH57 pKa = 7.75 DD58 pKa = 5.1 YY59 pKa = 9.52 YY60 pKa = 11.28 TDD62 pKa = 5.1 IITFDD67 pKa = 3.69 YY68 pKa = 11.04 GDD70 pKa = 4.1 EE71 pKa = 4.2 EE72 pKa = 4.65 VLSGDD77 pKa = 3.15 IFISIDD83 pKa = 3.4 RR84 pKa = 11.84 VSDD87 pKa = 3.38 NAFEE91 pKa = 4.12 YY92 pKa = 11.05 SSDD95 pKa = 3.54 FEE97 pKa = 5.84 AEE99 pKa = 4.0 LGRR102 pKa = 11.84 VMIHH106 pKa = 6.6 GLLHH110 pKa = 4.95 MMGYY114 pKa = 9.76 KK115 pKa = 10.45 DD116 pKa = 3.24 KK117 pKa = 11.16 TEE119 pKa = 4.1 EE120 pKa = 4.1 EE121 pKa = 4.14 EE122 pKa = 4.02 QEE124 pKa = 3.95 MRR126 pKa = 11.84 NKK128 pKa = 10.28 EE129 pKa = 4.14 DD130 pKa = 3.24 EE131 pKa = 4.17 CLQLIFNDD139 pKa = 3.7 SDD141 pKa = 3.52 EE142 pKa = 4.59 EE143 pKa = 4.16 EE144 pKa = 4.16
Molecular weight: 17.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.605
ProMoST 3.923
Dawson 3.77
Bjellqvist 3.961
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.516
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.643
EMBOSS 3.694
Sillero 3.923
Patrickios 0.846
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|I3ZZS0|I3ZZS0_ORNRL Uncharacterized protein OS=Ornithobacterium rhinotracheale (strain ATCC 51463 / DSM 15997 / CCUG 23171 / CIP 104009 / LMG 9086) OX=867902 GN=Ornrh_1012 PE=4 SV=1
MM1 pKa = 7.84 PSGKK5 pKa = 9.32 KK6 pKa = 9.55 RR7 pKa = 11.84 KK8 pKa = 7.23 RR9 pKa = 11.84 HH10 pKa = 5.2 KK11 pKa = 10.88 VSTHH15 pKa = 3.99 KK16 pKa = 10.28 RR17 pKa = 11.84 KK18 pKa = 9.7 KK19 pKa = 8.81 RR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 LNRR25 pKa = 11.84 HH26 pKa = 5.27 KK27 pKa = 10.6 KK28 pKa = 9.72 KK29 pKa = 10.58 KK30 pKa = 10.03
Molecular weight: 3.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.082
IPC_protein 12.618
Toseland 12.808
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.793
Wikipedia 13.276
Rodwell 12.735
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.808
DTASelect 12.793
Thurlkill 12.808
EMBOSS 13.29
Sillero 12.808
Patrickios 12.457
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.03
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2138
0
2138
688764
30
2361
322.2
36.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.628 ± 0.051
0.769 ± 0.016
5.311 ± 0.032
6.984 ± 0.069
5.185 ± 0.049
6.385 ± 0.05
1.729 ± 0.026
7.453 ± 0.047
8.438 ± 0.052
9.265 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.269 ± 0.023
6.034 ± 0.058
3.586 ± 0.029
3.855 ± 0.031
3.657 ± 0.032
5.995 ± 0.044
5.031 ± 0.032
6.12 ± 0.042
1.063 ± 0.018
4.244 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here