Capnocytophaga canis
Average proteome isoelectric point is 6.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2202 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B7HU15|A0A0B7HU15_9FLAO Glutamate dehydrogenase OS=Capnocytophaga canis OX=1848903 GN=gdhA PE=3 SV=1
MM1 pKa = 7.65 IYY3 pKa = 10.38 KK4 pKa = 9.91 FRR6 pKa = 11.84 IILDD10 pKa = 3.39 VKK12 pKa = 10.4 EE13 pKa = 4.54 DD14 pKa = 3.64 VFRR17 pKa = 11.84 DD18 pKa = 3.34 IAIEE22 pKa = 4.15 GDD24 pKa = 3.29 ATLEE28 pKa = 4.1 DD29 pKa = 3.74 LHH31 pKa = 8.32 NIIVQSFGFAGDD43 pKa = 3.71 EE44 pKa = 3.98 MASFYY49 pKa = 10.78 LTDD52 pKa = 3.85 EE53 pKa = 4.61 DD54 pKa = 4.31 WNQGEE59 pKa = 4.98 EE60 pKa = 3.77 ITLFDD65 pKa = 4.32 LSEE68 pKa = 4.37 SGEE71 pKa = 3.9 IRR73 pKa = 11.84 LMEE76 pKa = 4.34 EE77 pKa = 3.66 TSIDD81 pKa = 3.67 SVVSEE86 pKa = 4.87 EE87 pKa = 4.24 EE88 pKa = 3.81 PKK90 pKa = 10.83 LLYY93 pKa = 10.8 VYY95 pKa = 10.53 DD96 pKa = 4.85 FFSMWTFFVEE106 pKa = 4.23 LVEE109 pKa = 5.17 IADD112 pKa = 3.89 EE113 pKa = 4.45 YY114 pKa = 10.79 EE115 pKa = 4.27 NGVSYY120 pKa = 9.62 PALLFSHH127 pKa = 6.21 GTIPAEE133 pKa = 4.09 APEE136 pKa = 4.26 KK137 pKa = 10.8 LFEE140 pKa = 4.48 AEE142 pKa = 4.44 NFNEE146 pKa = 5.12 FDD148 pKa = 5.33 DD149 pKa = 4.37 EE150 pKa = 5.31 FNDD153 pKa = 3.7 FDD155 pKa = 4.43 MDD157 pKa = 3.78 EE158 pKa = 4.04 EE159 pKa = 5.37 DD160 pKa = 3.72 YY161 pKa = 11.64 EE162 pKa = 4.33 NFRR165 pKa = 11.84 EE166 pKa = 3.86 EE167 pKa = 4.86 DD168 pKa = 4.04 YY169 pKa = 11.37 YY170 pKa = 11.94
Molecular weight: 20.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.813
IPC2_protein 3.605
IPC_protein 3.579
Toseland 3.389
ProMoST 3.719
Dawson 3.554
Bjellqvist 3.706
Wikipedia 3.452
Rodwell 3.414
Grimsley 3.3
Solomon 3.541
Lehninger 3.49
Nozaki 3.668
DTASelect 3.834
Thurlkill 3.427
EMBOSS 3.465
Sillero 3.694
Patrickios 1.774
IPC_peptide 3.541
IPC2_peptide 3.681
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|A0A0B7HWM9|A0A0B7HWM9_9FLAO Uncharacterized protein OS=Capnocytophaga canis OX=1848903 GN=CCAND38_420082 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.13 RR10 pKa = 11.84 KK11 pKa = 9.48 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.49 HH16 pKa = 3.94 GFRR19 pKa = 11.84 EE20 pKa = 4.19 RR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 8.93 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.08 GRR39 pKa = 11.84 KK40 pKa = 8.3 KK41 pKa = 9.83 LTVSSEE47 pKa = 3.9 PRR49 pKa = 11.84 HH50 pKa = 5.77 KK51 pKa = 10.61 KK52 pKa = 9.84
Molecular weight: 6.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2202
0
2202
706585
22
4054
320.9
36.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.982 ± 0.054
0.756 ± 0.015
5.112 ± 0.035
6.877 ± 0.054
5.432 ± 0.043
6.144 ± 0.045
1.862 ± 0.022
7.91 ± 0.053
8.035 ± 0.056
9.144 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.303 ± 0.023
5.865 ± 0.043
3.328 ± 0.03
3.781 ± 0.034
3.829 ± 0.031
6.306 ± 0.038
5.587 ± 0.061
6.44 ± 0.039
1.056 ± 0.02
4.251 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here