Candidatus Glomeribacter gigasporarum BEG34

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Candidatus Glomeribacter; Candidatus Glomeribacter gigasporarum

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2JB08|G2JB08_9BURK Uncharacterized protein OS=Candidatus Glomeribacter gigasporarum BEG34 OX=1070319 GN=CAGGBEG34_340005 PE=4 SV=1
MM1 pKa = 7.62IEE3 pKa = 3.84GAGNFGYY10 pKa = 10.59NAATGEE16 pKa = 4.29YY17 pKa = 9.58GDD19 pKa = 4.94LMQAGVVDD27 pKa = 3.92PTKK30 pKa = 10.02VTRR33 pKa = 11.84TALQNAASVAGLLLMTDD50 pKa = 4.18CAVCEE55 pKa = 4.23LPKK58 pKa = 10.77DD59 pKa = 3.78DD60 pKa = 4.37TPAPGSMPGGGMGSMGMDD78 pKa = 3.22MM79 pKa = 5.48

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2JC37|G2JC37_9BURK Putative bacteriophage tail protein GpP Mu-like OS=Candidatus Glomeribacter gigasporarum BEG34 OX=1070319 GN=CAGGBEG34_900001 PE=4 SV=1
MM1 pKa = 8.01IGQLWMAEE9 pKa = 4.14FAQVRR14 pKa = 11.84QRR16 pKa = 11.84SGAQTRR22 pKa = 11.84QRR24 pKa = 11.84SGAQVARR31 pKa = 11.84VVTNVVMMGMGEE43 pKa = 3.98PLLNFDD49 pKa = 3.9AVVRR53 pKa = 11.84ALRR56 pKa = 11.84LMLDD60 pKa = 3.52DD61 pKa = 3.86NAYY64 pKa = 8.67GLSRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84VTVSTSGVVPMMDD83 pKa = 4.04RR84 pKa = 11.84LAQQLPVSLAVSLHH98 pKa = 6.3APDD101 pKa = 5.15DD102 pKa = 4.03ALRR105 pKa = 11.84DD106 pKa = 3.67ALVPLNKK113 pKa = 9.91KK114 pKa = 10.01YY115 pKa = 10.22PLRR118 pKa = 11.84EE119 pKa = 3.96LMRR122 pKa = 11.84ACQRR126 pKa = 11.84YY127 pKa = 9.06LKK129 pKa = 10.34AAPRR133 pKa = 11.84DD134 pKa = 4.09FITFEE139 pKa = 4.0YY140 pKa = 10.88CMLAGINDD148 pKa = 4.01HH149 pKa = 6.51EE150 pKa = 4.39KK151 pKa = 10.42HH152 pKa = 5.95ARR154 pKa = 11.84QLLALVQEE162 pKa = 5.05IPCKK166 pKa = 10.53FNLIPFNPFPEE177 pKa = 4.47SGFTRR182 pKa = 11.84SAPEE186 pKa = 3.55QIRR189 pKa = 11.84RR190 pKa = 11.84FAQILSARR198 pKa = 11.84GIVTTIRR205 pKa = 11.84KK206 pKa = 7.42TRR208 pKa = 11.84GDD210 pKa = 4.95DD211 pKa = 2.93IDD213 pKa = 3.99AACGQLAGIVNDD225 pKa = 3.88RR226 pKa = 11.84THH228 pKa = 6.78LAMRR232 pKa = 11.84NRR234 pKa = 11.84TAQIQAARR242 pKa = 11.84RR243 pKa = 11.84TITIHH248 pKa = 5.33PQQ250 pKa = 2.68

Molecular weight: 28.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1471

0

1471

429475

31

5058

292.0

32.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.212 ± 0.111

1.219 ± 0.025

4.862 ± 0.043

5.43 ± 0.055

3.753 ± 0.037

6.946 ± 0.072

2.586 ± 0.042

5.625 ± 0.052

3.851 ± 0.051

10.663 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.034

2.998 ± 0.047

4.921 ± 0.05

4.56 ± 0.048

7.432 ± 0.071

5.489 ± 0.049

4.886 ± 0.042

6.179 ± 0.057

1.369 ± 0.028

2.616 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski