Nicotiana tabacum (Common tobacco)
Average proteome isoelectric point is 6.77
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 73607 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1S3X1K5|A0A1S3X1K5_TOBAC protein Hook homolog OS=Nicotiana tabacum OX=4097 GN=LOC107760066 PE=4 SV=1
MM1 pKa = 7.2 RR2 pKa = 11.84 RR3 pKa = 11.84 KK4 pKa = 9.65 VRR6 pKa = 11.84 SLSDD10 pKa = 3.36 LYY12 pKa = 11.18 RR13 pKa = 11.84 RR14 pKa = 11.84 IPKK17 pKa = 9.66 CASSSVSADD26 pKa = 3.43 PQSPSINISAAQAGTGTEE44 pKa = 4.22 VATTEE49 pKa = 4.01 QLTPEE54 pKa = 3.98 MDD56 pKa = 3.8 LPIEE60 pKa = 4.39 LDD62 pKa = 3.42 LPAFFTQADD71 pKa = 4.32 FPVLPSFEE79 pKa = 4.23 QTTDD83 pKa = 3.26 PFDD86 pKa = 4.93 DD87 pKa = 4.69 LFDD90 pKa = 5.19 NICEE94 pKa = 3.98 QIIAIVQPFPEE105 pKa = 4.21 FQGWDD110 pKa = 3.63 LYY112 pKa = 11.42 FLDD115 pKa = 4.53 EE116 pKa = 4.83 VISLSLFSLMDD127 pKa = 3.54 EE128 pKa = 4.63 YY129 pKa = 11.55 YY130 pKa = 10.84 FSIEE134 pKa = 4.13 CLQGIYY140 pKa = 10.45 LDD142 pKa = 5.59 LITNGASSAFLTFFLNYY159 pKa = 10.27
Molecular weight: 17.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.757
IPC_protein 3.732
Toseland 3.516
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.656
Rodwell 3.554
Grimsley 3.427
Solomon 3.706
Lehninger 3.656
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.846
Patrickios 0.896
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.765
Protein with the highest isoelectric point:
>tr|Q5MA29|Q5MA29_TOBAC Reverse transcriptase Ty1/copia-type domain-containing protein OS=Nicotiana tabacum OX=4097 GN=orf152 PE=4 SV=1
MM1 pKa = 7.1 RR2 pKa = 11.84 AKK4 pKa = 9.27 WKK6 pKa = 9.53 KK7 pKa = 9.88 KK8 pKa = 8.64 RR9 pKa = 11.84 MRR11 pKa = 11.84 RR12 pKa = 11.84 LKK14 pKa = 10.08 RR15 pKa = 11.84 KK16 pKa = 8.21 RR17 pKa = 11.84 RR18 pKa = 11.84 KK19 pKa = 8.46 MRR21 pKa = 11.84 QRR23 pKa = 11.84 SKK25 pKa = 11.41
Molecular weight: 3.43 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.169
IPC_protein 12.749
Toseland 12.91
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.705
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.427
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.118
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61676
11931
73607
29996979
7
4876
407.5
45.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.578 ± 0.008
1.858 ± 0.004
5.288 ± 0.006
6.649 ± 0.015
4.202 ± 0.007
6.249 ± 0.008
2.353 ± 0.004
5.5 ± 0.006
6.436 ± 0.01
9.631 ± 0.012
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.429 ± 0.004
4.627 ± 0.006
4.8 ± 0.009
3.799 ± 0.007
5.2 ± 0.006
8.888 ± 0.011
4.921 ± 0.004
6.437 ± 0.007
1.265 ± 0.004
2.887 ± 0.005
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here