Gordonia phage Catfish
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A385D1F9|A0A385D1F9_9CAUD Tail fiber protein OS=Gordonia phage Catfish OX=2301538 GN=17 PE=4 SV=1
MM1 pKa = 7.55 HH2 pKa = 8.33 DD3 pKa = 3.81 YY4 pKa = 10.59 RR5 pKa = 11.84 HH6 pKa = 6.08 SATFAGVAEE15 pKa = 4.34 SGPLEE20 pKa = 4.18 GAEE23 pKa = 4.42 AFDD26 pKa = 5.17 LVPPLYY32 pKa = 10.88 SRR34 pKa = 11.84 DD35 pKa = 3.34 VTDD38 pKa = 3.45 YY39 pKa = 10.6 FDD41 pKa = 3.63 RR42 pKa = 11.84 VYY44 pKa = 10.52 VTVDD48 pKa = 3.57 DD49 pKa = 5.01 VPDD52 pKa = 3.92 EE53 pKa = 4.37 NDD55 pKa = 3.48 PDD57 pKa = 3.89 VSTKK61 pKa = 8.31 EE62 pKa = 3.81 TAVYY66 pKa = 10.24 GLAGTIEE73 pKa = 4.29 GQLAAIRR80 pKa = 11.84 LGGYY84 pKa = 9.48 VGGAEE89 pKa = 3.99 PAQALRR95 pKa = 11.84 LMGYY99 pKa = 10.42 ACDD102 pKa = 3.94 CC103 pKa = 4.36
Molecular weight: 11.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.84
IPC2_protein 4.024
IPC_protein 3.973
Toseland 3.77
ProMoST 4.139
Dawson 3.961
Bjellqvist 4.126
Wikipedia 3.897
Rodwell 3.808
Grimsley 3.681
Solomon 3.948
Lehninger 3.91
Nozaki 4.088
DTASelect 4.304
Thurlkill 3.834
EMBOSS 3.91
Sillero 4.101
Patrickios 0.655
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.968
Protein with the highest isoelectric point:
>tr|A0A385D1R1|A0A385D1R1_9CAUD Uncharacterized protein OS=Gordonia phage Catfish OX=2301538 GN=24 PE=4 SV=1
MM1 pKa = 6.46 TAKK4 pKa = 10.35 HH5 pKa = 6.16 AGRR8 pKa = 11.84 STRR11 pKa = 11.84 AWRR14 pKa = 11.84 RR15 pKa = 11.84 LKK17 pKa = 10.55 AAFRR21 pKa = 11.84 RR22 pKa = 11.84 KK23 pKa = 9.81 CADD26 pKa = 2.84 ARR28 pKa = 11.84 AVCWLCGQPIDD39 pKa = 3.99 YY40 pKa = 10.47 ALEE43 pKa = 4.48 HH44 pKa = 5.66 PHH46 pKa = 7.08 PYY48 pKa = 10.54 AFEE51 pKa = 5.1 PDD53 pKa = 3.64 HH54 pKa = 5.46 YY55 pKa = 10.62 HH56 pKa = 6.83 PVATHH61 pKa = 5.7 PHH63 pKa = 6.1 LAEE66 pKa = 5.01 DD67 pKa = 4.2 PANLRR72 pKa = 11.84 PSHH75 pKa = 6.7 ADD77 pKa = 3.15 CNKK80 pKa = 9.7 RR81 pKa = 11.84 RR82 pKa = 11.84 GDD84 pKa = 3.55 RR85 pKa = 11.84 APTFGLGRR93 pKa = 11.84 QSRR96 pKa = 11.84 KK97 pKa = 6.43 WW98 pKa = 3.25
Molecular weight: 11.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.296
IPC2_protein 9.311
IPC_protein 9.648
Toseland 10.116
ProMoST 9.838
Dawson 10.306
Bjellqvist 10.043
Wikipedia 10.482
Rodwell 10.54
Grimsley 10.365
Solomon 10.365
Lehninger 10.335
Nozaki 10.218
DTASelect 9.999
Thurlkill 10.175
EMBOSS 10.526
Sillero 10.248
Patrickios 10.277
IPC_peptide 10.365
IPC2_peptide 9.282
IPC2.peptide.svr19 8.188
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
78
0
78
14657
36
1429
187.9
20.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.144 ± 0.589
0.832 ± 0.118
6.734 ± 0.301
6.42 ± 0.325
2.975 ± 0.174
9.634 ± 0.43
1.815 ± 0.144
3.582 ± 0.16
3.493 ± 0.285
7.826 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.026 ± 0.084
2.872 ± 0.188
4.796 ± 0.218
2.9 ± 0.138
7.791 ± 0.384
5.499 ± 0.294
6.086 ± 0.219
8.085 ± 0.196
1.904 ± 0.115
2.586 ± 0.248
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here