Thermoplasmatales archaeon E-plasma

Taxonomy: cellular organisms; Archaea; Candidatus Thermoplasmatota; Thermoplasmata; Thermoplasmatales; unclassified Thermoplasmatales

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0MR89|T0MR89_9ARCH Proline--tRNA ligase OS=Thermoplasmatales archaeon E-plasma OX=667137 GN=proS PE=3 SV=1
MM1 pKa = 7.36AKK3 pKa = 10.04EE4 pKa = 3.88YY5 pKa = 9.6TPYY8 pKa = 10.1MVIEE12 pKa = 4.79AFPTNPICFHH22 pKa = 6.1SLEE25 pKa = 4.22NVLYY29 pKa = 10.74NDD31 pKa = 3.84VNGMDD36 pKa = 3.58IDD38 pKa = 4.04QDD40 pKa = 4.03VLLKK44 pKa = 10.83DD45 pKa = 3.56PEE47 pKa = 4.3YY48 pKa = 10.43MVMSEE53 pKa = 4.22ALLFGMKK60 pKa = 10.1VLIAEE65 pKa = 4.69RR66 pKa = 11.84FLATLL71 pKa = 4.09

Molecular weight: 8.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0MP68|T0MP68_9ARCH Uncharacterized protein OS=Thermoplasmatales archaeon E-plasma OX=667137 GN=AMDU2_EPLC00013G0003 PE=4 SV=1
MM1 pKa = 7.28TSSPSFRR8 pKa = 11.84RR9 pKa = 11.84EE10 pKa = 3.73LPTSKK15 pKa = 10.46VAGGISMGLNSPRR28 pKa = 11.84PEE30 pKa = 4.29GNLSFMLSHH39 pKa = 6.73LTLRR43 pKa = 11.84MQARR47 pKa = 11.84MKK49 pKa = 10.41QYY51 pKa = 10.81AIRR54 pKa = 11.84PLSLLSASGQINILRR69 pKa = 11.84EE70 pKa = 4.07IITFSLAFIPYY81 pKa = 8.59LAKK84 pKa = 11.01GEE86 pKa = 4.14FPLANVKK93 pKa = 10.11LCSLRR98 pKa = 11.84VSSLFKK104 pKa = 11.04DD105 pKa = 3.14RR106 pKa = 11.84VTEE109 pKa = 4.0LRR111 pKa = 11.84TCGASIGVYY120 pKa = 10.09VFLRR124 pKa = 11.84SNFCFSFMFFPMFLKK139 pKa = 10.57PPNQLLRR146 pKa = 11.84CPSRR150 pKa = 11.84NKK152 pKa = 9.06PTTEE156 pKa = 4.07VVGSS160 pKa = 3.71

Molecular weight: 17.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1661

0

1661

480563

24

1714

289.3

32.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.673 ± 0.053

0.703 ± 0.019

4.881 ± 0.056

6.672 ± 0.071

4.959 ± 0.056

7.106 ± 0.053

1.531 ± 0.023

9.389 ± 0.055

6.922 ± 0.074

8.859 ± 0.068

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.025

5.325 ± 0.066

3.648 ± 0.037

2.425 ± 0.03

4.559 ± 0.055

8.238 ± 0.088

5.174 ± 0.056

6.155 ± 0.04

0.84 ± 0.017

4.011 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski