Bifidobacterium magnum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1507 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087BCG6|A0A087BCG6_9BIFI Peptidyl-tRNA hydrolase OS=Bifidobacterium magnum OX=1692 GN=pth PE=3 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.47YY5 pKa = 10.14DD6 pKa = 4.13SPRR9 pKa = 11.84NTNDD13 pKa = 2.97EE14 pKa = 4.5DD15 pKa = 4.19EE16 pKa = 5.2EE17 pKa = 4.47SLQALSKK24 pKa = 10.34SSHH27 pKa = 5.15STATDD32 pKa = 3.11MDD34 pKa = 4.67DD35 pKa = 4.6DD36 pKa = 4.49EE37 pKa = 4.83NAIAEE42 pKa = 4.35DD43 pKa = 4.23YY44 pKa = 9.11EE45 pKa = 5.05LPGADD50 pKa = 5.03LSNEE54 pKa = 4.12DD55 pKa = 3.43ASVTVVPMQGDD66 pKa = 3.63EE67 pKa = 5.07FICAEE72 pKa = 4.18CFLVKK77 pKa = 10.27HH78 pKa = 6.45RR79 pKa = 11.84SQLAYY84 pKa = 9.9MNEE87 pKa = 4.4DD88 pKa = 3.45GQPVCQEE95 pKa = 4.08CAAA98 pKa = 4.29

Molecular weight: 10.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087BAY8|A0A087BAY8_9BIFI TPR_REGION domain-containing protein OS=Bifidobacterium magnum OX=1692 GN=BMAGN_0137 PE=4 SV=1
MM1 pKa = 7.52VVITHH6 pKa = 6.13VIVWSRR12 pKa = 11.84RR13 pKa = 11.84FPCVSVLAIVPHH25 pKa = 5.8SFARR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 8.1VVAMSDD38 pKa = 3.55EE39 pKa = 4.32QTPQPQWHH47 pKa = 6.75LVPEE51 pKa = 4.77HH52 pKa = 6.39MATPARR58 pKa = 11.84VPQPQPVATPRR69 pKa = 11.84DD70 pKa = 3.63PQAPEE75 pKa = 4.47DD76 pKa = 3.86LASQLVQVDD85 pKa = 3.7PLLNRR90 pKa = 11.84PRR92 pKa = 11.84RR93 pKa = 11.84SSMVLCIVFGVLFLALGGLAYY114 pKa = 9.67WLGVHH119 pKa = 5.76TVLGQSYY126 pKa = 9.56EE127 pKa = 4.19DD128 pKa = 3.62QVITSYY134 pKa = 11.2ASRR137 pKa = 11.84MPAFLSSTAFPFSNSLVVIITSLVMGALALVVALVRR173 pKa = 11.84KK174 pKa = 9.29RR175 pKa = 11.84WWLAVQLAGFIVVAFIASRR194 pKa = 11.84VLKK197 pKa = 10.01PVLPRR202 pKa = 11.84PFLIHH207 pKa = 6.33VEE209 pKa = 4.13SSTTNSAPSGHH220 pKa = 6.55VMMAAIASVALLCAVPRR237 pKa = 11.84VWRR240 pKa = 11.84AACALIGVLFTLSVGFSVVTMRR262 pKa = 11.84WHH264 pKa = 6.37RR265 pKa = 11.84PIDD268 pKa = 3.4VVMAVFITSGLVLLMLACTARR289 pKa = 11.84SGMDD293 pKa = 2.95RR294 pKa = 11.84PGKK297 pKa = 9.55RR298 pKa = 11.84VPSASVQIVASALIALGVCAYY319 pKa = 10.37AYY321 pKa = 7.28ATYY324 pKa = 10.17VIWEE328 pKa = 4.09ISLGLSAVAAWTARR342 pKa = 11.84GADD345 pKa = 3.78DD346 pKa = 4.13SATALIVGTVCVVFGLVLSLRR367 pKa = 11.84QLTASPLSKK376 pKa = 9.84MGLIGTPPTPPKK388 pKa = 10.22RR389 pKa = 11.84RR390 pKa = 11.84SS391 pKa = 3.09

Molecular weight: 42.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1507

0

1507

531363

22

2086

352.6

38.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.529 ± 0.081

1.004 ± 0.02

6.39 ± 0.054

6.031 ± 0.063

3.452 ± 0.036

7.49 ± 0.054

2.445 ± 0.032

5.497 ± 0.053

3.911 ± 0.053

8.689 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.936 ± 0.026

3.557 ± 0.041

4.614 ± 0.033

3.827 ± 0.04

5.813 ± 0.068

5.862 ± 0.047

5.979 ± 0.048

7.838 ± 0.046

1.33 ± 0.026

2.806 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski