Pseudoxanthomonas dokdonensis
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3001 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R0CQP3|A0A0R0CQP3_9GAMM Nucleotide-binding protein ABB29_14395 OS=Pseudoxanthomonas dokdonensis OX=344882 GN=ABB29_14395 PE=3 SV=1
MM1 pKa = 7.43 KK2 pKa = 10.33 SVNRR6 pKa = 11.84 RR7 pKa = 11.84 AALVASLLLVLPFATLAQDD26 pKa = 3.87 AEE28 pKa = 4.44 AEE30 pKa = 4.26 AEE32 pKa = 4.29 AEE34 pKa = 4.06 ASSPISWSLGATTDD48 pKa = 3.49 YY49 pKa = 11.21 VFRR52 pKa = 11.84 GLSQTDD58 pKa = 3.36 EE59 pKa = 4.62 GPAFQAGATYY69 pKa = 7.88 TAPFGMYY76 pKa = 10.19 AGVWGSNVDD85 pKa = 4.58 FGPGDD90 pKa = 3.62 PNYY93 pKa = 10.53 EE94 pKa = 3.97 LDD96 pKa = 3.56 TFIGYY101 pKa = 7.68 NTDD104 pKa = 3.82 FGDD107 pKa = 3.73 VVNFDD112 pKa = 3.39 IMANRR117 pKa = 11.84 YY118 pKa = 8.45 NYY120 pKa = 9.88 PDD122 pKa = 3.9 AGKK125 pKa = 10.25 SNYY128 pKa = 8.87 WEE130 pKa = 5.42 FIAKK134 pKa = 8.98 TSFWEE139 pKa = 4.4 NYY141 pKa = 8.89 SVTVGYY147 pKa = 9.27 TDD149 pKa = 5.42 DD150 pKa = 4.27 VFNSDD155 pKa = 2.88 TDD157 pKa = 3.02 GWYY160 pKa = 10.04 YY161 pKa = 11.3 AVGASFPLPADD172 pKa = 3.04 ISLDD176 pKa = 3.48 LSAGKK181 pKa = 10.14 SEE183 pKa = 4.45 FEE185 pKa = 4.47 DD186 pKa = 4.74 GEE188 pKa = 4.3 VGRR191 pKa = 11.84 DD192 pKa = 3.54 YY193 pKa = 10.92 MDD195 pKa = 2.95 WSVALGKK202 pKa = 10.52 SFGPLTVTAAYY213 pKa = 9.65 IGTDD217 pKa = 2.99 GDD219 pKa = 3.68 GRR221 pKa = 11.84 DD222 pKa = 3.79 AYY224 pKa = 10.9 GDD226 pKa = 3.46 IADD229 pKa = 4.36 DD230 pKa = 4.04 RR231 pKa = 11.84 FVLSASVGMM240 pKa = 4.72
Molecular weight: 25.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.49
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.834
Patrickios 0.807
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.752
Protein with the highest isoelectric point:
>tr|A0A0R0CW75|A0A0R0CW75_9GAMM Endoribonuclease OS=Pseudoxanthomonas dokdonensis OX=344882 GN=ABB29_07275 PE=3 SV=1
MM1 pKa = 7.5 AMKK4 pKa = 10.25 KK5 pKa = 9.72 AAKK8 pKa = 9.66 KK9 pKa = 10.38 KK10 pKa = 8.49 PAKK13 pKa = 10.09 KK14 pKa = 8.96 ATKK17 pKa = 9.71 KK18 pKa = 9.79 AAKK21 pKa = 9.69 KK22 pKa = 10.2 AVAKK26 pKa = 9.01 KK27 pKa = 7.44 TAKK30 pKa = 10.18 KK31 pKa = 9.81 AAKK34 pKa = 9.54 KK35 pKa = 9.06 VAKK38 pKa = 10.15 KK39 pKa = 10.22 KK40 pKa = 8.81 VAKK43 pKa = 10.08 KK44 pKa = 9.05 VAKK47 pKa = 9.98 KK48 pKa = 9.19 ATRR51 pKa = 11.84 KK52 pKa = 8.54 VAKK55 pKa = 10.1 KK56 pKa = 8.95 ATKK59 pKa = 9.74 KK60 pKa = 9.18 VAKK63 pKa = 10.14 KK64 pKa = 9.97 RR65 pKa = 11.84 VAKK68 pKa = 10.06 AAKK71 pKa = 9.54 KK72 pKa = 9.99 KK73 pKa = 9.06 VAKK76 pKa = 10.09 KK77 pKa = 9.17 VAKK80 pKa = 10.2 KK81 pKa = 9.04 ATKK84 pKa = 9.74 KK85 pKa = 9.18 VAKK88 pKa = 10.14 KK89 pKa = 9.97 RR90 pKa = 11.84 VAKK93 pKa = 10.06 AAKK96 pKa = 9.54 KK97 pKa = 9.99 KK98 pKa = 9.06 VAKK101 pKa = 10.15 KK102 pKa = 9.14 VAKK105 pKa = 10.09 KK106 pKa = 9.88 AAKK109 pKa = 9.54 KK110 pKa = 8.91 VAKK113 pKa = 10.21 KK114 pKa = 10.07 RR115 pKa = 11.84 VAKK118 pKa = 9.81 VAKK121 pKa = 9.91 KK122 pKa = 10.11 KK123 pKa = 9.19 VAKK126 pKa = 10.02 KK127 pKa = 9.05 VAKK130 pKa = 10.23 KK131 pKa = 10.16 AVKK134 pKa = 9.85 KK135 pKa = 9.88 AAKK138 pKa = 9.78 KK139 pKa = 10.26 AVAKK143 pKa = 10.52 KK144 pKa = 10.21 AGKK147 pKa = 9.52 KK148 pKa = 8.91 AAKK151 pKa = 9.53 KK152 pKa = 10.03 AVRR155 pKa = 11.84 KK156 pKa = 8.08 VARR159 pKa = 11.84 RR160 pKa = 11.84 KK161 pKa = 9.42 KK162 pKa = 9.26 AAPVALPATPAPLII176 pKa = 4.24
Molecular weight: 18.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 11.389
IPC_protein 12.632
Toseland 12.925
ProMoST 13.29
Dawson 12.939
Bjellqvist 12.837
Wikipedia 13.32
Rodwell 13.408
Grimsley 12.969
Solomon 13.334
Lehninger 13.247
Nozaki 12.91
DTASelect 12.837
Thurlkill 12.91
EMBOSS 13.393
Sillero 12.91
Patrickios 13.115
IPC_peptide 13.349
IPC2_peptide 12.281
IPC2.peptide.svr19 8.937
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3001
0
3001
1002130
34
3821
333.9
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.479 ± 0.063
0.802 ± 0.016
6.051 ± 0.04
5.059 ± 0.042
3.335 ± 0.026
8.401 ± 0.047
2.255 ± 0.023
4.349 ± 0.033
2.779 ± 0.037
10.837 ± 0.069
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.264 ± 0.02
2.777 ± 0.037
5.206 ± 0.038
4.58 ± 0.033
7.13 ± 0.053
5.746 ± 0.038
4.732 ± 0.053
7.209 ± 0.036
1.559 ± 0.02
2.451 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here