Dethiosulfatibacter aminovorans DSM 17477

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellia incertae sedis; Dethiosulfatibacter; Dethiosulfatibacter aminovorans

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6ISX7|A0A1M6ISX7_9FIRM Uncharacterized protein OS=Dethiosulfatibacter aminovorans DSM 17477 OX=1121476 GN=SAMN02745751_02422 PE=4 SV=1
MM1 pKa = 7.19KK2 pKa = 9.94RR3 pKa = 11.84KK4 pKa = 9.23SRR6 pKa = 11.84AVIYY10 pKa = 10.35VILAVILLLSTFVRR24 pKa = 11.84AYY26 pKa = 10.72GDD28 pKa = 3.54YY29 pKa = 9.97GTGNLSSDD37 pKa = 3.58GQQEE41 pKa = 4.38KK42 pKa = 10.6IGITEE47 pKa = 4.01YY48 pKa = 11.3LNDD51 pKa = 3.64QIIVKK56 pKa = 10.17FKK58 pKa = 11.23DD59 pKa = 4.2DD60 pKa = 4.39ISDD63 pKa = 3.58TDD65 pKa = 3.21RR66 pKa = 11.84KK67 pKa = 10.35LIKK70 pKa = 10.01KK71 pKa = 9.4YY72 pKa = 11.02YY73 pKa = 10.28KK74 pKa = 10.7LIDD77 pKa = 3.35KK78 pKa = 10.88AKK80 pKa = 8.51FKK82 pKa = 11.22SGGEE86 pKa = 3.99VLKK89 pKa = 11.2LKK91 pKa = 10.98GNLTVEE97 pKa = 4.66EE98 pKa = 4.22LVEE101 pKa = 3.96MLGEE105 pKa = 4.28LDD107 pKa = 3.65SVEE110 pKa = 4.24YY111 pKa = 10.96AGFDD115 pKa = 3.73YY116 pKa = 10.87IMRR119 pKa = 11.84ATDD122 pKa = 4.03FPDD125 pKa = 4.5DD126 pKa = 3.82PWDD129 pKa = 4.16GDD131 pKa = 3.79SARR134 pKa = 11.84YY135 pKa = 8.16LWGMEE140 pKa = 4.32KK141 pKa = 9.27IDD143 pKa = 3.97ANGDD147 pKa = 3.54TEE149 pKa = 6.03YY150 pKa = 10.78PGAWNLATGNPEE162 pKa = 3.99VIVAVIDD169 pKa = 3.46TGVDD173 pKa = 3.06MGHH176 pKa = 6.49VDD178 pKa = 3.68LAEE181 pKa = 4.56NIWINEE187 pKa = 3.86VEE189 pKa = 4.3LNGDD193 pKa = 3.81AGVDD197 pKa = 3.67DD198 pKa = 5.66DD199 pKa = 5.1GNGYY203 pKa = 9.68IDD205 pKa = 6.01DD206 pKa = 3.63VHH208 pKa = 7.62GWDD211 pKa = 3.81FHH213 pKa = 8.45NGDD216 pKa = 4.71GDD218 pKa = 3.79PTDD221 pKa = 4.14DD222 pKa = 3.78QGHH225 pKa = 5.73GTHH228 pKa = 7.07CSGTIAAAANGIGVAGVAPNVNVMPLKK255 pKa = 10.68FLGSDD260 pKa = 3.59GSGSFSDD267 pKa = 3.43AVLAIEE273 pKa = 4.24YY274 pKa = 10.03AEE276 pKa = 4.62AMGADD281 pKa = 4.37VISASWGGYY290 pKa = 7.71TIGVDD295 pKa = 3.04SSMYY299 pKa = 10.55DD300 pKa = 3.51AINDD304 pKa = 3.71FSGLFCAAAGNDD316 pKa = 4.22GYY318 pKa = 11.64DD319 pKa = 3.12MDD321 pKa = 4.53FYY323 pKa = 11.81GMFGYY328 pKa = 9.92HH329 pKa = 6.76HH330 pKa = 7.44APATYY335 pKa = 8.91PNEE338 pKa = 4.43NIVSVASIDD347 pKa = 3.69SDD349 pKa = 3.7GSLSYY354 pKa = 10.35FSNYY358 pKa = 8.78GRR360 pKa = 11.84TSVDD364 pKa = 3.12LVSPGRR370 pKa = 11.84SILSTVPGDD379 pKa = 3.53SYY381 pKa = 11.44GYY383 pKa = 10.82KK384 pKa = 10.1SGTSMATPHH393 pKa = 5.82VAGTVALMISHH404 pKa = 7.75DD405 pKa = 3.49IDD407 pKa = 4.61LNGSVTRR414 pKa = 11.84SKK416 pKa = 11.17VQLKK420 pKa = 10.64DD421 pKa = 3.64DD422 pKa = 4.08LLEE425 pKa = 4.16ATVYY429 pKa = 10.6KK430 pKa = 9.61STYY433 pKa = 9.73NSVVLDD439 pKa = 3.89GCLNAYY445 pKa = 7.82EE446 pKa = 4.75ALLLTTDD453 pKa = 4.51NGTEE457 pKa = 4.04PPPPPEE463 pKa = 5.31NEE465 pKa = 4.26DD466 pKa = 3.88PYY468 pKa = 11.4NDD470 pKa = 3.75TDD472 pKa = 3.99FPPYY476 pKa = 8.19MTGTFEE482 pKa = 4.07VGGAIIAHH490 pKa = 6.25TGTWIDD496 pKa = 4.29EE497 pKa = 4.23GDD499 pKa = 3.56LDD501 pKa = 5.74YY502 pKa = 11.75YY503 pKa = 9.11CTWQVTADD511 pKa = 3.98PEE513 pKa = 4.22EE514 pKa = 5.03DD515 pKa = 3.59SPSDD519 pKa = 3.56YY520 pKa = 11.31EE521 pKa = 3.92EE522 pKa = 4.82WYY524 pKa = 10.48DD525 pKa = 4.23EE526 pKa = 3.94SWLYY530 pKa = 11.27EE531 pKa = 4.16EE532 pKa = 5.38FPPIFIVEE540 pKa = 4.38PGQAEE545 pKa = 4.18KK546 pKa = 10.81YY547 pKa = 10.03IRR549 pKa = 11.84IEE551 pKa = 3.96VVAVDD556 pKa = 3.91EE557 pKa = 4.73EE558 pKa = 4.63GLDD561 pKa = 3.72GVAYY565 pKa = 10.54SDD567 pKa = 3.86WEE569 pKa = 4.39WIEE572 pKa = 3.98PEE574 pKa = 4.57APNEE578 pKa = 3.97PPVNDD583 pKa = 3.49GDD585 pKa = 4.06RR586 pKa = 11.84PSVTGLMIAGEE597 pKa = 4.3TVYY600 pKa = 11.05AEE602 pKa = 4.31DD603 pKa = 5.39GGWSDD608 pKa = 5.07DD609 pKa = 3.71GGVEE613 pKa = 4.01NLEE616 pKa = 3.96YY617 pKa = 10.86SYY619 pKa = 10.05MWQISDD625 pKa = 4.12TEE627 pKa = 4.26FGTYY631 pKa = 10.12EE632 pKa = 4.15DD633 pKa = 3.94TGVTADD639 pKa = 3.95SYY641 pKa = 11.63SLTEE645 pKa = 4.0AEE647 pKa = 4.04VTKK650 pKa = 9.9FIRR653 pKa = 11.84VLVTAIDD660 pKa = 3.63NGDD663 pKa = 3.82PVEE666 pKa = 4.01MSEE669 pKa = 4.17PSEE672 pKa = 4.34SPGRR676 pKa = 11.84QVIPNAAPVNDD687 pKa = 3.81ITPSITGTLIAGDD700 pKa = 3.53IAYY703 pKa = 10.24ADD705 pKa = 3.49IGDD708 pKa = 3.72WSDD711 pKa = 4.69DD712 pKa = 3.49RR713 pKa = 11.84GEE715 pKa = 4.28EE716 pKa = 4.02NLSYY720 pKa = 10.82SYY722 pKa = 10.79NWKK725 pKa = 10.12IKK727 pKa = 9.98EE728 pKa = 4.01LDD730 pKa = 3.35GSYY733 pKa = 11.09SDD735 pKa = 5.31IGGSDD740 pKa = 3.64NPLSLPEE747 pKa = 4.84SDD749 pKa = 2.51TGYY752 pKa = 10.39YY753 pKa = 9.66IKK755 pKa = 11.09LEE757 pKa = 4.0VTATDD762 pKa = 3.46KK763 pKa = 11.39GDD765 pKa = 3.71PVKK768 pKa = 9.17WTSEE772 pKa = 3.72SSADD776 pKa = 3.79YY777 pKa = 10.47LVEE780 pKa = 4.06PAEE783 pKa = 4.33TPVINTFTSSITDD796 pKa = 4.08AYY798 pKa = 8.01PTSIRR803 pKa = 11.84NKK805 pKa = 6.99WTATVTVSTNLPMSEE820 pKa = 4.66AEE822 pKa = 4.67VAYY825 pKa = 9.44EE826 pKa = 3.77WSDD829 pKa = 3.54GKK831 pKa = 11.21SVTAGTAPTINGEE844 pKa = 4.35CTVISDD850 pKa = 5.48PIRR853 pKa = 11.84KK854 pKa = 9.35NIGSITFTIIGITPLDD870 pKa = 3.85NDD872 pKa = 3.29SDD874 pKa = 4.38YY875 pKa = 11.49TFDD878 pKa = 4.48PAHH881 pKa = 7.03SDD883 pKa = 3.23LTVTVNKK890 pKa = 9.87PP891 pKa = 2.85

Molecular weight: 96.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6LG06|A0A1M6LG06_9FIRM Uncharacterized protein OS=Dethiosulfatibacter aminovorans DSM 17477 OX=1121476 GN=SAMN02745751_03162 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.7TSMKK7 pKa = 10.25VKK9 pKa = 9.39QQRR12 pKa = 11.84KK13 pKa = 6.65QKK15 pKa = 10.28FSTRR19 pKa = 11.84EE20 pKa = 3.53YY21 pKa = 10.22SRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 7.1AYY33 pKa = 9.15LRR35 pKa = 11.84KK36 pKa = 9.67YY37 pKa = 9.37GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.14GQIPGVRR57 pKa = 11.84KK58 pKa = 10.13ASWW61 pKa = 2.78

Molecular weight: 7.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3628

0

3628

1182095

39

2390

325.8

36.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.475 ± 0.047

1.168 ± 0.015

5.982 ± 0.036

7.826 ± 0.046

4.304 ± 0.03

7.294 ± 0.041

1.529 ± 0.014

8.98 ± 0.041

7.415 ± 0.043

8.764 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.286 ± 0.022

4.977 ± 0.035

3.084 ± 0.022

2.124 ± 0.017

3.951 ± 0.026

6.181 ± 0.03

4.955 ± 0.03

7.05 ± 0.032

0.709 ± 0.013

3.945 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski