Chilli leaf curl Sri Lanka virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4F5F8|K4F5F8_9GEMI Transcriptional activator protein OS=Chilli leaf curl Sri Lanka virus OX=2560381 GN=C2 PE=3 SV=1
MM1 pKa = 7.45GLCISMPSSSSRR13 pKa = 11.84VKK15 pKa = 10.49PSSEE19 pKa = 3.98TPDD22 pKa = 2.68ISMSLTLTPHH32 pKa = 6.72PNSIQTSRR40 pKa = 11.84EE41 pKa = 3.94LSPHH45 pKa = 6.22PMSSPTSRR53 pKa = 11.84RR54 pKa = 11.84TVITSTGVIFSSMEE68 pKa = 3.99DD69 pKa = 3.17LLEE72 pKa = 4.25EE73 pKa = 4.32VNRR76 pKa = 11.84QLMMLQQRR84 pKa = 11.84PSIQDD89 pKa = 3.33PLMPLWQQ96 pKa = 4.11

Molecular weight: 10.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4F967|K4F967_9GEMI Replication enhancer OS=Chilli leaf curl Sri Lanka virus OX=2560381 GN=C3 PE=3 SV=1
MM1 pKa = 8.1DD2 pKa = 4.85LRR4 pKa = 11.84TGEE7 pKa = 4.9RR8 pKa = 11.84ITAAQATNGVSILQVPNPLYY28 pKa = 10.69FKK30 pKa = 10.46ILSHH34 pKa = 7.37DD35 pKa = 3.64SRR37 pKa = 11.84PFNSNTDD44 pKa = 4.36LITIRR49 pKa = 11.84VQFNHH54 pKa = 6.34NLRR57 pKa = 11.84KK58 pKa = 9.8ALGIHH63 pKa = 6.0KK64 pKa = 10.16CFLTFRR70 pKa = 11.84IWTTLQPQTGHH81 pKa = 6.5FLRR84 pKa = 11.84VFRR87 pKa = 11.84TQVLKK92 pKa = 10.92CINNFAVISLNNVIRR107 pKa = 11.84AVDD110 pKa = 3.51HH111 pKa = 5.93VLWNVLTQTVYY122 pKa = 10.45VQSSNIIKK130 pKa = 10.4FNVYY134 pKa = 9.89

Molecular weight: 15.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1091

96

374

181.8

20.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.5 ± 0.928

1.833 ± 0.376

4.95 ± 0.548

3.941 ± 0.76

4.216 ± 0.719

5.408 ± 0.732

3.85 ± 0.366

5.683 ± 0.704

5.775 ± 0.943

6.874 ± 0.98

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.75 ± 0.788

5.316 ± 0.594

6.416 ± 0.633

6.049 ± 0.964

6.233 ± 0.846

8.433 ± 1.114

5.225 ± 0.868

6.691 ± 0.964

1.283 ± 0.172

3.575 ± 0.542
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski