Cryptosporangium arvum DSM 44712
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4033 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A010ZSE2|A0A010ZSE2_9ACTN Carbohydrate ABC transporter membrane protein 1 CUT1 family OS=Cryptosporangium arvum DSM 44712 OX=927661 GN=CryarDRAFT_2727 PE=3 SV=1
MM1 pKa = 7.12 SALVKK6 pKa = 10.38 RR7 pKa = 11.84 LAAAGSLIAAGAALVLVPSPAYY29 pKa = 10.46 ADD31 pKa = 3.65 TVTVNYY37 pKa = 10.23 SCEE40 pKa = 3.95 IPLSGTQTGDD50 pKa = 3.32 VEE52 pKa = 4.64 VTITAPATATVGEE65 pKa = 4.57 TVDD68 pKa = 3.52 VTVTTGPIPILPPIDD83 pKa = 4.79 LDD85 pKa = 4.09 AGSVTPSGEE94 pKa = 4.16 VTVSGAQTGAVAVTGTPNPEE114 pKa = 4.26 PYY116 pKa = 9.66 PANQPVQLTALTGQLTLTTAGEE138 pKa = 4.4 VQLSPGAVNATATTIFGTFTIPCVPTATPTPVAAVISVSS177 pKa = 3.15
Molecular weight: 17.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.91
IPC2_protein 3.668
IPC_protein 3.516
Toseland 3.35
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.478
Rodwell 3.376
Grimsley 3.274
Solomon 3.452
Lehninger 3.414
Nozaki 3.681
DTASelect 3.795
Thurlkill 3.439
EMBOSS 3.49
Sillero 3.643
Patrickios 0.172
IPC_peptide 3.452
IPC2_peptide 3.605
IPC2.peptide.svr19 3.646
Protein with the highest isoelectric point:
>tr|A0A010ZQ45|A0A010ZQ45_9ACTN Kef-type K+ ransport system predicted NAD-binding component OS=Cryptosporangium arvum DSM 44712 OX=927661 GN=CryarDRAFT_1859 PE=4 SV=1
MM1 pKa = 7.21 VSTVRR6 pKa = 11.84 KK7 pKa = 8.51 IRR9 pKa = 11.84 TVRR12 pKa = 11.84 TVSDD16 pKa = 3.24 VHH18 pKa = 4.84 TVRR21 pKa = 11.84 NIRR24 pKa = 11.84 TSRR27 pKa = 11.84 SVCTMSTVRR36 pKa = 11.84 ISRR39 pKa = 11.84 NVRR42 pKa = 11.84 TSRR45 pKa = 11.84 NVCTMSTVRR54 pKa = 11.84 TSRR57 pKa = 11.84 NVRR60 pKa = 11.84 TMSTVRR66 pKa = 11.84 TSHH69 pKa = 5.87 NVRR72 pKa = 11.84 TMSTVRR78 pKa = 11.84 TTRR81 pKa = 11.84 NVRR84 pKa = 11.84 TMRR87 pKa = 11.84 ALRR90 pKa = 11.84 FVRR93 pKa = 11.84 PVRR96 pKa = 11.84 LIPALRR102 pKa = 11.84 HH103 pKa = 4.96 VSTLRR108 pKa = 11.84 HH109 pKa = 5.62 VGTLHH114 pKa = 6.7 FVRR117 pKa = 11.84 INGKK121 pKa = 9.22 RR122 pKa = 11.84 LSEE125 pKa = 4.0 RR126 pKa = 11.84 DD127 pKa = 3.1 AQRR130 pKa = 11.84 ILRR133 pKa = 11.84 RR134 pKa = 11.84 LHH136 pKa = 5.96 RR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 SLRR142 pKa = 11.84 RR143 pKa = 11.84 RR144 pKa = 11.84 AVLRR148 pKa = 11.84 GTVQQRR154 pKa = 11.84 TLRR157 pKa = 11.84 RR158 pKa = 11.84 PTLQRR163 pKa = 11.84 RR164 pKa = 11.84 TLRR167 pKa = 11.84 RR168 pKa = 11.84 RR169 pKa = 11.84 SAHH172 pKa = 4.94 EE173 pKa = 3.67 LRR175 pKa = 11.84 RR176 pKa = 11.84 GKK178 pKa = 7.92 QLRR181 pKa = 11.84 RR182 pKa = 11.84 SRR184 pKa = 11.84 SRR186 pKa = 11.84 LKK188 pKa = 10.54 IGWIGHH194 pKa = 4.43 TVRR197 pKa = 11.84 LRR199 pKa = 11.84 RR200 pKa = 11.84 VVNKK204 pKa = 9.7 PPAPISDD211 pKa = 3.81 HH212 pKa = 5.67 PRR214 pKa = 11.84 SRR216 pKa = 11.84 ILQCRR221 pKa = 11.84 RR222 pKa = 11.84 VLQRR226 pKa = 11.84 GRR228 pKa = 11.84 PGLHH232 pKa = 4.52 VRR234 pKa = 11.84 IRR236 pKa = 11.84 RR237 pKa = 11.84 QAEE240 pKa = 3.82 IRR242 pKa = 11.84 PGPPRR247 pKa = 11.84 PLPPPIAVPVNEE259 pKa = 4.11 QQRR262 pKa = 11.84 PGHH265 pKa = 4.76 SRR267 pKa = 11.84 RR268 pKa = 11.84 RR269 pKa = 11.84 RR270 pKa = 11.84 RR271 pKa = 11.84 TLRR274 pKa = 3.46
Molecular weight: 32.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 10.965
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.281
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 11.989
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.147
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4033
0
4033
1372967
13
14508
340.4
36.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.16 ± 0.06
0.659 ± 0.01
6.159 ± 0.036
5.206 ± 0.03
2.767 ± 0.023
9.222 ± 0.05
2.059 ± 0.018
3.312 ± 0.036
1.613 ± 0.03
10.735 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.509 ± 0.016
1.715 ± 0.023
6.077 ± 0.043
2.487 ± 0.022
8.252 ± 0.047
5.2 ± 0.028
6.101 ± 0.032
9.342 ± 0.05
1.509 ± 0.017
1.915 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here