Rhodopirellula solitaria
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5210 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C5XXD9|A0A5C5XXD9_9BACT Putative cadmium-transporting ATPase OS=Rhodopirellula solitaria OX=2527987 GN=cadA PE=3 SV=1
MM1 pKa = 7.45 SEE3 pKa = 4.24 SEE5 pKa = 5.08 FIAEE9 pKa = 4.12 LTVVLDD15 pKa = 4.23 GQSDD19 pKa = 3.71 TARR22 pKa = 11.84 AQIPLLLASLPEE34 pKa = 4.02 PATRR38 pKa = 11.84 LDD40 pKa = 3.71 LQVFPAQDD48 pKa = 3.24 GDD50 pKa = 3.9 GFFTVRR56 pKa = 11.84 ASVDD60 pKa = 3.44 GPNLYY65 pKa = 10.49 VINKK69 pKa = 10.12 AIDD72 pKa = 3.43 TYY74 pKa = 12.01 ADD76 pKa = 3.81 LFDD79 pKa = 5.17 AKK81 pKa = 9.7 YY82 pKa = 9.25 TEE84 pKa = 5.04 NGVQPPIPIVDD95 pKa = 4.46 CFDD98 pKa = 2.72 VDD100 pKa = 3.97 YY101 pKa = 10.04 PVNDD105 pKa = 3.34 IVVDD109 pKa = 4.56 CAANWLRR116 pKa = 11.84 TVWQSLGNIEE126 pKa = 4.22 CRR128 pKa = 11.84 VPVVIVGNDD137 pKa = 3.55 GYY139 pKa = 10.86 GTVTPVEE146 pKa = 4.2 LHH148 pKa = 6.43 SGAAAA153 pKa = 3.66
Molecular weight: 16.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.729
IPC2_protein 3.795
IPC_protein 3.783
Toseland 3.567
ProMoST 3.961
Dawson 3.795
Bjellqvist 3.948
Wikipedia 3.745
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.91
Patrickios 1.901
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.813
Protein with the highest isoelectric point:
>tr|A0A5C5YIP2|A0A5C5YIP2_9BACT Uncharacterized protein OS=Rhodopirellula solitaria OX=2527987 GN=CA85_00160 PE=4 SV=1
MM1 pKa = 7.62 AKK3 pKa = 10.07 KK4 pKa = 10.24 KK5 pKa = 9.64 ATGVKK10 pKa = 9.85 KK11 pKa = 10.18 SAKK14 pKa = 9.55 KK15 pKa = 9.12 RR16 pKa = 11.84 TAKK19 pKa = 10.4 KK20 pKa = 9.64 SAKK23 pKa = 9.94 KK24 pKa = 9.97 SVKK27 pKa = 9.76 KK28 pKa = 10.4 RR29 pKa = 11.84 AATKK33 pKa = 10.11 KK34 pKa = 9.45 AAKK37 pKa = 9.97 KK38 pKa = 10.22 KK39 pKa = 8.49 ATKK42 pKa = 10.27 KK43 pKa = 10.4 KK44 pKa = 8.55 ATKK47 pKa = 10.25 KK48 pKa = 10.39 KK49 pKa = 8.55 ATKK52 pKa = 10.25 KK53 pKa = 10.39 KK54 pKa = 8.55 ATKK57 pKa = 10.25 KK58 pKa = 10.39 KK59 pKa = 8.66 ATKK62 pKa = 10.05 KK63 pKa = 9.96 KK64 pKa = 9.64 AAKK67 pKa = 9.99 KK68 pKa = 7.91 STRR71 pKa = 11.84 KK72 pKa = 9.86 KK73 pKa = 8.76 ATKK76 pKa = 10.08 KK77 pKa = 10.15 KK78 pKa = 9.57 AAKK81 pKa = 9.88 KK82 pKa = 9.8 KK83 pKa = 8.93 AAKK86 pKa = 9.68 KK87 pKa = 9.88 KK88 pKa = 10.19 RR89 pKa = 11.84 RR90 pKa = 3.59
Molecular weight: 9.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.377
IPC2_protein 11.199
IPC_protein 12.457
Toseland 12.749
ProMoST 13.13
Dawson 12.749
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 13.203
Grimsley 12.778
Solomon 13.159
Lehninger 13.071
Nozaki 12.735
DTASelect 12.661
Thurlkill 12.735
EMBOSS 13.203
Sillero 12.735
Patrickios 12.91
IPC_peptide 13.159
IPC2_peptide 12.091
IPC2.peptide.svr19 8.814
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5210
0
5210
1953092
29
7638
374.9
41.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.089 ± 0.037
1.158 ± 0.018
6.503 ± 0.037
6.101 ± 0.03
3.519 ± 0.019
7.72 ± 0.044
2.237 ± 0.022
4.96 ± 0.02
3.086 ± 0.037
9.448 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.021
3.009 ± 0.031
5.381 ± 0.023
4.086 ± 0.022
6.904 ± 0.038
6.979 ± 0.029
5.64 ± 0.035
7.1 ± 0.027
1.496 ± 0.018
2.33 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here