Rhodopirellula solitaria

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes;

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A5C5XXD9|A0A5C5XXD9_9BACT Putative cadmium-transporting ATPase OS=Rhodopirellula solitaria OX=2527987 GN=cadA PE=3 SV=1
MM1 pKa = 7.45SEE3 pKa = 4.24SEE5 pKa = 5.08FIAEE9 pKa = 4.12LTVVLDD15 pKa = 4.23GQSDD19 pKa = 3.71TARR22 pKa = 11.84AQIPLLLASLPEE34 pKa = 4.02PATRR38 pKa = 11.84LDD40 pKa = 3.71LQVFPAQDD48 pKa = 3.24GDD50 pKa = 3.9GFFTVRR56 pKa = 11.84ASVDD60 pKa = 3.44GPNLYY65 pKa = 10.49VINKK69 pKa = 10.12AIDD72 pKa = 3.43TYY74 pKa = 12.01ADD76 pKa = 3.81LFDD79 pKa = 5.17AKK81 pKa = 9.7YY82 pKa = 9.25TEE84 pKa = 5.04NGVQPPIPIVDD95 pKa = 4.46CFDD98 pKa = 2.72VDD100 pKa = 3.97YY101 pKa = 10.04PVNDD105 pKa = 3.34IVVDD109 pKa = 4.56CAANWLRR116 pKa = 11.84TVWQSLGNIEE126 pKa = 4.22CRR128 pKa = 11.84VPVVIVGNDD137 pKa = 3.55GYY139 pKa = 10.86GTVTPVEE146 pKa = 4.2LHH148 pKa = 6.43SGAAAA153 pKa = 3.66

Molecular weight:
16.5 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A5C5YIP2|A0A5C5YIP2_9BACT Uncharacterized protein OS=Rhodopirellula solitaria OX=2527987 GN=CA85_00160 PE=4 SV=1
MM1 pKa = 7.62AKK3 pKa = 10.07KK4 pKa = 10.24KK5 pKa = 9.64ATGVKK10 pKa = 9.85KK11 pKa = 10.18SAKK14 pKa = 9.55KK15 pKa = 9.12RR16 pKa = 11.84TAKK19 pKa = 10.4KK20 pKa = 9.64SAKK23 pKa = 9.94KK24 pKa = 9.97SVKK27 pKa = 9.76KK28 pKa = 10.4RR29 pKa = 11.84AATKK33 pKa = 10.11KK34 pKa = 9.45AAKK37 pKa = 9.97KK38 pKa = 10.22KK39 pKa = 8.49ATKK42 pKa = 10.27KK43 pKa = 10.4KK44 pKa = 8.55ATKK47 pKa = 10.25KK48 pKa = 10.39KK49 pKa = 8.55ATKK52 pKa = 10.25KK53 pKa = 10.39KK54 pKa = 8.55ATKK57 pKa = 10.25KK58 pKa = 10.39KK59 pKa = 8.66ATKK62 pKa = 10.05KK63 pKa = 9.96KK64 pKa = 9.64AAKK67 pKa = 9.99KK68 pKa = 7.91STRR71 pKa = 11.84KK72 pKa = 9.86KK73 pKa = 8.76ATKK76 pKa = 10.08KK77 pKa = 10.15KK78 pKa = 9.57AAKK81 pKa = 9.88KK82 pKa = 9.8KK83 pKa = 8.93AAKK86 pKa = 9.68KK87 pKa = 9.88KK88 pKa = 10.19RR89 pKa = 11.84RR90 pKa = 3.59

Molecular weight:
9.93 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5210

0

5210

1953092

29

7638

374.9

41.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.089 ± 0.037

1.158 ± 0.018

6.503 ± 0.037

6.101 ± 0.03

3.519 ± 0.019

7.72 ± 0.044

2.237 ± 0.022

4.96 ± 0.02

3.086 ± 0.037

9.448 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.021

3.009 ± 0.031

5.381 ± 0.023

4.086 ± 0.022

6.904 ± 0.038

6.979 ± 0.029

5.64 ± 0.035

7.1 ± 0.027

1.496 ± 0.018

2.33 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski