Sphingomonas sp. AX6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A654A396|A0A654A396_9SPHN Sigma-fimbriae uncharacterized paralogous subunit OS=Sphingomonas sp. AX6 OX=2653171 GN=SPHINGOAX6_30121 PE=4 SV=1
MM1 pKa = 7.92RR2 pKa = 11.84FYY4 pKa = 10.75TPSLTALALAATATPAFAQDD24 pKa = 3.07TDD26 pKa = 3.66VPEE29 pKa = 4.38PVTVSSTITLTSDD42 pKa = 2.92YY43 pKa = 9.78RR44 pKa = 11.84FRR46 pKa = 11.84GITQSDD52 pKa = 3.91EE53 pKa = 4.14GPAAQAGIVVNHH65 pKa = 6.3EE66 pKa = 3.89SGFYY70 pKa = 9.5IGGWASSISNDD81 pKa = 3.25SLPGYY86 pKa = 10.61GEE88 pKa = 4.82AEE90 pKa = 4.08TNLFGGYY97 pKa = 7.54STEE100 pKa = 4.18TASGVGFDD108 pKa = 4.59VGLLYY113 pKa = 10.86YY114 pKa = 9.84LYY116 pKa = 10.01PDD118 pKa = 4.21AASGAQTDD126 pKa = 4.0FFEE129 pKa = 5.31PYY131 pKa = 10.53AAITYY136 pKa = 10.04SIGPVSTKK144 pKa = 10.58VGAAYY149 pKa = 10.03AWGGQDD155 pKa = 4.44GLDD158 pKa = 3.58FTGGKK163 pKa = 9.84DD164 pKa = 3.7DD165 pKa = 4.51NLYY168 pKa = 10.79LYY170 pKa = 10.82GDD172 pKa = 4.01ASVGIPTTPFSLNAHH187 pKa = 5.92VGRR190 pKa = 11.84SEE192 pKa = 4.46GSLALLNIDD201 pKa = 4.16SSDD204 pKa = 4.36VYY206 pKa = 10.23WDD208 pKa = 3.39WSAGVEE214 pKa = 4.15WSGGPIVGGLKK225 pKa = 10.8YY226 pKa = 10.2IDD228 pKa = 3.84TDD230 pKa = 3.16ISNDD234 pKa = 3.46GEE236 pKa = 4.34FAQSLGRR243 pKa = 11.84GSTVIGYY250 pKa = 9.94VGFSFF255 pKa = 5.06

Molecular weight: 26.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653YK69|A0A653YK69_9SPHN Uncharacterized protein OS=Sphingomonas sp. AX6 OX=2653171 GN=SPHINGOAX6_10102 PE=4 SV=1
MM1 pKa = 6.68QAARR5 pKa = 11.84SATRR9 pKa = 11.84RR10 pKa = 11.84GSGAGEE16 pKa = 4.19GAWDD20 pKa = 4.47GLSKK24 pKa = 10.91DD25 pKa = 3.77AGSAILPRR33 pKa = 11.84GCAAMADD40 pKa = 4.02EE41 pKa = 5.23NPARR45 pKa = 11.84MYY47 pKa = 10.9PFGNPDD53 pKa = 3.44GVKK56 pKa = 10.04HH57 pKa = 6.52LYY59 pKa = 10.11SLSRR63 pKa = 11.84CLPLTRR69 pKa = 11.84LSRR72 pKa = 11.84VAFEE76 pKa = 4.69LVEE79 pKa = 3.68KK80 pKa = 10.33AYY82 pKa = 10.36RR83 pKa = 11.84GRR85 pKa = 11.84GAITHH90 pKa = 6.23AVSIRR95 pKa = 11.84VLDD98 pKa = 4.28FARR101 pKa = 11.84TLLDD105 pKa = 3.3TNGFEE110 pKa = 4.63RR111 pKa = 11.84KK112 pKa = 9.22RR113 pKa = 11.84VVTRR117 pKa = 11.84PLRR120 pKa = 11.84PPHH123 pKa = 6.51AAPPALL129 pKa = 4.06

Molecular weight: 13.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3153

0

3153

934631

20

2132

296.4

31.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.328 ± 0.066

0.743 ± 0.012

6.269 ± 0.035

5.367 ± 0.036

3.478 ± 0.029

9.002 ± 0.043

1.953 ± 0.023

5.123 ± 0.028

2.801 ± 0.037

9.476 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.496 ± 0.023

2.542 ± 0.026

5.309 ± 0.033

3.02 ± 0.025

7.614 ± 0.046

5.184 ± 0.03

5.429 ± 0.037

7.357 ± 0.037

1.428 ± 0.019

2.081 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski