Amino acid dipepetide frequency for Sphingomonas sp. AX6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.525AlaAla: 18.525 ± 0.219	1.067AlaCys: 1.067 ± 0.04	7.819AlaAsp: 7.819 ± 0.104	7.566AlaGlu: 7.566 ± 0.108	4.264AlaPhe: 4.264 ± 0.078	11.001AlaGly: 11.001 ± 0.113	2.357AlaHis: 2.357 ± 0.054	7.218AlaIle: 7.218 ± 0.093	4.179AlaLys: 4.179 ± 0.079	13.648AlaLeu: 13.648 ± 0.152	4.045AlaMet: 4.045 ± 0.077	3.382AlaAsn: 3.382 ± 0.072	6.485AlaPro: 6.485 ± 0.103	4.447AlaGln: 4.447 ± 0.078	10.193AlaArg: 10.193 ± 0.133	6.835AlaSer: 6.835 ± 0.092	7.221AlaThr: 7.221 ± 0.1	8.999AlaVal: 8.999 ± 0.107	1.637AlaTrp: 1.637 ± 0.052	2.39AlaTyr: 2.39 ± 0.046	0.0AlaXaa: 0.0 ± 0.0
Cys	0.927CysAla: 0.927 ± 0.035	0.078CysCys: 0.078 ± 0.01	0.532CysAsp: 0.532 ± 0.025	0.378CysGlu: 0.378 ± 0.019	0.265CysPhe: 0.265 ± 0.018	0.873CysGly: 0.873 ± 0.036	0.188CysHis: 0.188 ± 0.016	0.336CysIle: 0.336 ± 0.021	0.148CysLys: 0.148 ± 0.014	0.611CysLeu: 0.611 ± 0.024	0.125CysMet: 0.125 ± 0.012	0.205CysAsn: 0.205 ± 0.016	0.429CysPro: 0.429 ± 0.025	0.172CysGln: 0.172 ± 0.014	0.579CysArg: 0.579 ± 0.03	0.397CysSer: 0.397 ± 0.021	0.382CysThr: 0.382 ± 0.022	0.564CysVal: 0.564 ± 0.023	0.106CysTrp: 0.106 ± 0.012	0.132CysTyr: 0.132 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	8.874AspAla: 8.874 ± 0.122	0.454AspCys: 0.454 ± 0.023	3.823AspAsp: 3.823 ± 0.09	3.386AspGlu: 3.386 ± 0.068	2.148AspPhe: 2.148 ± 0.054	5.965AspGly: 5.965 ± 0.095	1.378AspHis: 1.378 ± 0.04	2.896AspIle: 2.896 ± 0.06	1.623AspLys: 1.623 ± 0.043	5.738AspLeu: 5.738 ± 0.08	1.48AspMet: 1.48 ± 0.04	1.382AspAsn: 1.382 ± 0.039	3.719AspPro: 3.719 ± 0.066	1.978AspGln: 1.978 ± 0.044	5.527AspArg: 5.527 ± 0.089	2.263AspSer: 2.263 ± 0.048	2.948AspThr: 2.948 ± 0.066	4.35AspVal: 4.35 ± 0.073	1.169AspTrp: 1.169 ± 0.035	1.585AspTyr: 1.585 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	7.73GluAla: 7.73 ± 0.106	0.322GluCys: 0.322 ± 0.019	2.683GluAsp: 2.683 ± 0.072	2.653GluGlu: 2.653 ± 0.076	1.51GluPhe: 1.51 ± 0.047	4.586GluGly: 4.586 ± 0.069	1.096GluHis: 1.096 ± 0.039	2.922GluIle: 2.922 ± 0.06	1.772GluLys: 1.772 ± 0.052	4.766GluLeu: 4.766 ± 0.079	1.465GluMet: 1.465 ± 0.04	1.356GluAsn: 1.356 ± 0.039	2.662GluPro: 2.662 ± 0.066	1.878GluGln: 1.878 ± 0.047	5.4GluArg: 5.4 ± 0.084	2.323GluSer: 2.323 ± 0.049	3.1GluThr: 3.1 ± 0.053	3.634GluVal: 3.634 ± 0.068	0.842GluTrp: 0.842 ± 0.025	0.976GluTyr: 0.976 ± 0.035	0.0GluXaa: 0.0 ± 0.0
Phe	5.033PheAla: 5.033 ± 0.074	0.258PheCys: 0.258 ± 0.017	2.768PheAsp: 2.768 ± 0.06	2.039PheGlu: 2.039 ± 0.047	1.229PhePhe: 1.229 ± 0.039	3.566PheGly: 3.566 ± 0.071	0.659PheHis: 0.659 ± 0.027	1.395PheIle: 1.395 ± 0.037	0.779PheLys: 0.779 ± 0.031	3.012PheLeu: 3.012 ± 0.054	0.704PheMet: 0.704 ± 0.027	0.938PheAsn: 0.938 ± 0.035	1.387PhePro: 1.387 ± 0.038	0.906PheGln: 0.906 ± 0.028	2.206PheArg: 2.206 ± 0.05	1.836PheSer: 1.836 ± 0.047	1.924PheThr: 1.924 ± 0.05	2.803PheVal: 2.803 ± 0.055	0.524PheTrp: 0.524 ± 0.028	0.816PheTyr: 0.816 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	10.105GlyAla: 10.105 ± 0.118	0.824GlyCys: 0.824 ± 0.028	5.663GlyAsp: 5.663 ± 0.099	5.06GlyGlu: 5.06 ± 0.08	3.623GlyPhe: 3.623 ± 0.063	8.504GlyGly: 8.504 ± 0.149	1.868GlyHis: 1.868 ± 0.047	4.68GlyIle: 4.68 ± 0.073	3.055GlyLys: 3.055 ± 0.069	8.292GlyLeu: 8.292 ± 0.111	2.414GlyMet: 2.414 ± 0.057	2.349GlyAsn: 2.349 ± 0.075	3.479GlyPro: 3.479 ± 0.07	3.133GlyGln: 3.133 ± 0.063	6.579GlyArg: 6.579 ± 0.083	4.844GlySer: 4.844 ± 0.084	4.929GlyThr: 4.929 ± 0.095	6.638GlyVal: 6.638 ± 0.093	1.765GlyTrp: 1.765 ± 0.052	2.216GlyTyr: 2.216 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.479HisAla: 2.479 ± 0.059	0.209HisCys: 0.209 ± 0.015	1.225HisAsp: 1.225 ± 0.04	0.963HisGlu: 0.963 ± 0.035	0.782HisPhe: 0.782 ± 0.031	1.863HisGly: 1.863 ± 0.048	0.592HisHis: 0.592 ± 0.028	0.905HisIle: 0.905 ± 0.032	0.424HisLys: 0.424 ± 0.021	1.832HisLeu: 1.832 ± 0.041	0.417HisMet: 0.417 ± 0.022	0.457HisAsn: 0.457 ± 0.023	1.325HisPro: 1.325 ± 0.039	0.547HisGln: 0.547 ± 0.023	1.586HisArg: 1.586 ± 0.048	0.951HisSer: 0.951 ± 0.031	0.687HisThr: 0.687 ± 0.025	1.439HisVal: 1.439 ± 0.044	0.324HisTrp: 0.324 ± 0.018	0.523HisTyr: 0.523 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.505IleAla: 8.505 ± 0.105	0.367IleCys: 0.367 ± 0.018	4.539IleAsp: 4.539 ± 0.072	3.721IleGlu: 3.721 ± 0.062	1.494IlePhe: 1.494 ± 0.041	5.322IleGly: 5.322 ± 0.084	0.912IleHis: 0.912 ± 0.03	1.832IleIle: 1.832 ± 0.052	1.07IleLys: 1.07 ± 0.037	4.022IleLeu: 4.022 ± 0.075	0.819IleMet: 0.819 ± 0.033	1.255IleAsn: 1.255 ± 0.035	2.297IlePro: 2.297 ± 0.05	1.193IleGln: 1.193 ± 0.042	3.238IleArg: 3.238 ± 0.063	2.381IleSer: 2.381 ± 0.049	2.523IleThr: 2.523 ± 0.049	4.294IleVal: 4.294 ± 0.074	0.555IleTrp: 0.555 ± 0.024	0.894IleTyr: 0.894 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.614LysAla: 3.614 ± 0.084	0.151LysCys: 0.151 ± 0.013	1.393LysAsp: 1.393 ± 0.048	1.117LysGlu: 1.117 ± 0.04	0.811LysPhe: 0.811 ± 0.027	2.342LysGly: 2.342 ± 0.06	0.553LysHis: 0.553 ± 0.028	1.287LysIle: 1.287 ± 0.036	0.998LysLys: 0.998 ± 0.046	3.023LysLeu: 3.023 ± 0.059	0.723LysMet: 0.723 ± 0.034	0.639LysAsn: 0.639 ± 0.03	1.94LysPro: 1.94 ± 0.052	0.81LysGln: 0.81 ± 0.034	2.531LysArg: 2.531 ± 0.051	1.597LysSer: 1.597 ± 0.039	1.623LysThr: 1.623 ± 0.049	1.962LysVal: 1.962 ± 0.055	0.368LysTrp: 0.368 ± 0.019	0.526LysTyr: 0.526 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	13.114LeuAla: 13.114 ± 0.147	0.712LeuCys: 0.712 ± 0.03	6.18LeuAsp: 6.18 ± 0.097	4.385LeuGlu: 4.385 ± 0.077	3.559LeuPhe: 3.559 ± 0.066	8.226LeuGly: 8.226 ± 0.098	1.703LeuHis: 1.703 ± 0.05	5.125LeuIle: 5.125 ± 0.087	2.77LeuLys: 2.77 ± 0.065	8.632LeuLeu: 8.632 ± 0.131	2.186LeuMet: 2.186 ± 0.048	2.431LeuAsn: 2.431 ± 0.049	5.443LeuPro: 5.443 ± 0.076	2.199LeuGln: 2.199 ± 0.055	6.48LeuArg: 6.48 ± 0.084	5.967LeuSer: 5.967 ± 0.09	5.497LeuThr: 5.497 ± 0.083	7.054LeuVal: 7.054 ± 0.098	1.188LeuTrp: 1.188 ± 0.039	1.909LeuTyr: 1.909 ± 0.044	0.0LeuXaa: 0.0 ± 0.0
Met	3.338MetAla: 3.338 ± 0.062	0.146MetCys: 0.146 ± 0.012	1.072MetAsp: 1.072 ± 0.038	0.95MetGlu: 0.95 ± 0.033	0.725MetPhe: 0.725 ± 0.032	2.009MetGly: 2.009 ± 0.054	0.444MetHis: 0.444 ± 0.02	1.47MetIle: 1.47 ± 0.042	0.929MetLys: 0.929 ± 0.034	2.684MetLeu: 2.684 ± 0.063	0.672MetMet: 0.672 ± 0.027	0.7MetAsn: 0.7 ± 0.023	1.51MetPro: 1.51 ± 0.042	0.7MetGln: 0.7 ± 0.029	2.03MetArg: 2.03 ± 0.039	1.459MetSer: 1.459 ± 0.042	1.9MetThr: 1.9 ± 0.047	1.716MetVal: 1.716 ± 0.044	0.248MetTrp: 0.248 ± 0.017	0.256MetTyr: 0.256 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	3.486AsnAla: 3.486 ± 0.069	0.241AsnCys: 0.241 ± 0.015	1.466AsnAsp: 1.466 ± 0.045	1.213AsnGlu: 1.213 ± 0.04	0.903AsnPhe: 0.903 ± 0.035	2.413AsnGly: 2.413 ± 0.066	0.489AsnHis: 0.489 ± 0.024	1.254AsnIle: 1.254 ± 0.032	0.576AsnLys: 0.576 ± 0.026	2.437AsnLeu: 2.437 ± 0.053	0.566AsnMet: 0.566 ± 0.026	0.656AsnAsn: 0.656 ± 0.032	1.792AsnPro: 1.792 ± 0.042	0.734AsnGln: 0.734 ± 0.026	1.936AsnArg: 1.936 ± 0.046	1.174AsnSer: 1.174 ± 0.036	1.104AsnThr: 1.104 ± 0.036	1.877AsnVal: 1.877 ± 0.057	0.444AsnTrp: 0.444 ± 0.021	0.656AsnTyr: 0.656 ± 0.029	0.0AsnXaa: 0.0 ± 0.0
Pro	6.618ProAla: 6.618 ± 0.101	0.322ProCys: 0.322 ± 0.02	3.921ProAsp: 3.921 ± 0.072	3.374ProGlu: 3.374 ± 0.071	1.947ProPhe: 1.947 ± 0.048	4.814ProGly: 4.814 ± 0.071	1.042ProHis: 1.042 ± 0.035	2.612ProIle: 2.612 ± 0.051	1.509ProLys: 1.509 ± 0.044	4.571ProLeu: 4.571 ± 0.076	1.329ProMet: 1.329 ± 0.041	1.397ProAsn: 1.397 ± 0.039	2.861ProPro: 2.861 ± 0.089	1.666ProGln: 1.666 ± 0.046	3.342ProArg: 3.342 ± 0.063	2.734ProSer: 2.734 ± 0.061	2.855ProThr: 2.855 ± 0.06	4.423ProVal: 4.423 ± 0.065	0.69ProTrp: 0.69 ± 0.029	1.062ProTyr: 1.062 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.005GlnAla: 4.005 ± 0.076	0.215GlnCys: 0.215 ± 0.016	1.326GlnAsp: 1.326 ± 0.037	1.203GlnGlu: 1.203 ± 0.035	1.132GlnPhe: 1.132 ± 0.034	2.463GlnGly: 2.463 ± 0.051	0.562GlnHis: 0.562 ± 0.027	1.701GlnIle: 1.701 ± 0.039	0.816GlnLys: 0.816 ± 0.028	2.832GlnLeu: 2.832 ± 0.062	0.866GlnMet: 0.866 ± 0.029	0.744GlnAsn: 0.744 ± 0.03	1.873GlnPro: 1.873 ± 0.048	1.106GlnGln: 1.106 ± 0.047	2.584GlnArg: 2.584 ± 0.052	1.787GlnSer: 1.787 ± 0.05	1.72GlnThr: 1.72 ± 0.049	2.269GlnVal: 2.269 ± 0.051	0.416GlnTrp: 0.416 ± 0.021	0.58GlnTyr: 0.58 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	9.472ArgAla: 9.472 ± 0.104	0.55ArgCys: 0.55 ± 0.025	4.831ArgAsp: 4.831 ± 0.084	4.074ArgGlu: 4.074 ± 0.079	3.185ArgPhe: 3.185 ± 0.069	5.771ArgGly: 5.771 ± 0.089	1.698ArgHis: 1.698 ± 0.044	4.532ArgIle: 4.532 ± 0.084	1.919ArgLys: 1.919 ± 0.042	7.896ArgLeu: 7.896 ± 0.108	2.155ArgMet: 2.155 ± 0.044	1.816ArgAsn: 1.816 ± 0.043	3.749ArgPro: 3.749 ± 0.07	2.42ArgGln: 2.42 ± 0.056	5.804ArgArg: 5.804 ± 0.1	3.829ArgSer: 3.829 ± 0.074	3.84ArgThr: 3.84 ± 0.07	5.497ArgVal: 5.497 ± 0.079	1.266ArgTrp: 1.266 ± 0.041	1.838ArgTyr: 1.838 ± 0.049	0.0ArgXaa: 0.0 ± 0.0
Ser	6.489SerAla: 6.489 ± 0.097	0.351SerCys: 0.351 ± 0.021	3.366SerAsp: 3.366 ± 0.06	2.596SerGlu: 2.596 ± 0.055	1.967SerPhe: 1.967 ± 0.042	5.671SerGly: 5.671 ± 0.108	0.863SerHis: 0.863 ± 0.032	2.73SerIle: 2.73 ± 0.052	1.313SerLys: 1.313 ± 0.039	4.801SerLeu: 4.801 ± 0.074	1.218SerMet: 1.218 ± 0.038	1.478SerAsn: 1.478 ± 0.041	2.82SerPro: 2.82 ± 0.053	1.412SerGln: 1.412 ± 0.036	3.542SerArg: 3.542 ± 0.063	2.679SerSer: 2.679 ± 0.068	2.78SerThr: 2.78 ± 0.074	3.788SerVal: 3.788 ± 0.061	0.748SerTrp: 0.748 ± 0.029	1.227SerTyr: 1.227 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.56ThrAla: 6.56 ± 0.097	0.362ThrCys: 0.362 ± 0.019	3.188ThrAsp: 3.188 ± 0.06	2.461ThrGlu: 2.461 ± 0.056	1.688ThrPhe: 1.688 ± 0.042	5.523ThrGly: 5.523 ± 0.123	1.03ThrHis: 1.03 ± 0.037	3.084ThrIle: 3.084 ± 0.057	1.328ThrLys: 1.328 ± 0.043	5.983ThrLeu: 5.983 ± 0.091	1.254ThrMet: 1.254 ± 0.037	1.392ThrAsn: 1.392 ± 0.042	3.64ThrPro: 3.64 ± 0.063	1.59ThrGln: 1.59 ± 0.042	3.778ThrArg: 3.778 ± 0.057	2.705ThrSer: 2.705 ± 0.064	2.909ThrThr: 2.909 ± 0.086	4.178ThrVal: 4.178 ± 0.08	0.547ThrTrp: 0.547 ± 0.023	1.089ThrTyr: 1.089 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	10.337ValAla: 10.337 ± 0.125	0.472ValCys: 0.472 ± 0.025	4.665ValAsp: 4.665 ± 0.08	4.908ValGlu: 4.908 ± 0.088	2.178ValPhe: 2.178 ± 0.054	5.982ValGly: 5.982 ± 0.094	1.322ValHis: 1.322 ± 0.041	3.799ValIle: 3.799 ± 0.067	1.902ValLys: 1.902 ± 0.057	6.34ValLeu: 6.34 ± 0.093	1.722ValMet: 1.722 ± 0.046	1.878ValAsn: 1.878 ± 0.058	3.914ValPro: 3.914 ± 0.068	2.077ValGln: 2.077 ± 0.051	5.482ValArg: 5.482 ± 0.081	4.108ValSer: 4.108 ± 0.072	4.647ValThr: 4.647 ± 0.087	5.715ValVal: 5.715 ± 0.083	0.847ValTrp: 0.847 ± 0.031	1.275ValTyr: 1.275 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	1.435TrpAla: 1.435 ± 0.04	0.129TrpCys: 0.129 ± 0.012	0.725TrpAsp: 0.725 ± 0.033	0.613TrpGlu: 0.613 ± 0.027	0.579TrpPhe: 0.579 ± 0.023	1.047TrpGly: 1.047 ± 0.034	0.322TrpHis: 0.322 ± 0.018	0.655TrpIle: 0.655 ± 0.024	0.42TrpLys: 0.42 ± 0.02	1.67TrpLeu: 1.67 ± 0.041	0.406TrpMet: 0.406 ± 0.019	0.41TrpAsn: 0.41 ± 0.017	0.703TrpPro: 0.703 ± 0.033	0.593TrpGln: 0.593 ± 0.029	1.42TrpArg: 1.42 ± 0.041	0.848TrpSer: 0.848 ± 0.035	0.821TrpThr: 0.821 ± 0.034	0.93TrpVal: 0.93 ± 0.039	0.262TrpTrp: 0.262 ± 0.017	0.296TrpTyr: 0.296 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.634TyrAla: 2.634 ± 0.056	0.199TyrCys: 0.199 ± 0.016	1.502TyrAsp: 1.502 ± 0.048	1.014TyrGlu: 1.014 ± 0.033	0.794TyrPhe: 0.794 ± 0.03	2.078TyrGly: 2.078 ± 0.056	0.449TyrHis: 0.449 ± 0.021	0.797TyrIle: 0.797 ± 0.032	0.48TyrLys: 0.48 ± 0.023	1.968TyrLeu: 1.968 ± 0.051	0.394TyrMet: 0.394 ± 0.021	0.552TyrAsn: 0.552 ± 0.028	1.06TyrPro: 1.06 ± 0.038	0.64TyrGln: 0.64 ± 0.027	1.908TyrArg: 1.908 ± 0.048	1.121TyrSer: 1.121 ± 0.033	0.879TyrThr: 0.879 ± 0.031	1.44TyrVal: 1.44 ± 0.044	0.337TyrTrp: 0.337 ± 0.02	0.567TyrTyr: 0.567 ± 0.027	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3153 proteins (934631 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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