Bernardetia litoralis (strain ATCC 23117 / DSM 6794 / NBRC 15988 / NCIMB 1366 / Sio-4) (Flexibacter litoralis)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Bernardetiaceae; Bernardetia; Bernardetia litoralis

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I4AKN2|I4AKN2_BERLS Uncharacterized protein OS=Bernardetia litoralis (strain ATCC 23117 / DSM 6794 / NBRC 15988 / NCIMB 1366 / Sio-4) OX=880071 GN=Fleli_2136 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.42VKK4 pKa = 10.44NKK6 pKa = 8.63KK7 pKa = 6.15TTISLANYY15 pKa = 8.72CSLYY19 pKa = 9.6IDD21 pKa = 4.8KK22 pKa = 10.74FNAHH26 pKa = 5.69QLTRR30 pKa = 11.84SWQRR34 pKa = 11.84KK35 pKa = 7.2AALAAATVATSAFLGGTTSAQTIPCAPEE63 pKa = 3.56YY64 pKa = 10.8AQTASALPITNPGDD78 pKa = 3.03ITTNGGYY85 pKa = 10.76ARR87 pKa = 11.84THH89 pKa = 5.69LVDD92 pKa = 3.41YY93 pKa = 11.23DD94 pKa = 3.85GDD96 pKa = 4.03GDD98 pKa = 4.22FDD100 pKa = 5.4LMIGEE105 pKa = 4.11KK106 pKa = 9.89SGRR109 pKa = 11.84EE110 pKa = 3.38LAYY113 pKa = 10.82YY114 pKa = 9.7EE115 pKa = 4.21NTGTTSAPIWSPAVNNPNGLNLIAGEE141 pKa = 4.15NGTASFFVDD150 pKa = 3.58IDD152 pKa = 3.62NDD154 pKa = 3.66GDD156 pKa = 3.83LDD158 pKa = 4.09AFLGTEE164 pKa = 3.91KK165 pKa = 10.89DD166 pKa = 3.26GLAFFEE172 pKa = 4.4NTGTRR177 pKa = 11.84TTPVYY182 pKa = 10.1AAPVTNPFGLDD193 pKa = 3.19TGLDD197 pKa = 3.58SYY199 pKa = 9.67LTPTFADD206 pKa = 3.52IDD208 pKa = 3.85NDD210 pKa = 3.77GDD212 pKa = 3.78FDD214 pKa = 6.52LITGEE219 pKa = 4.05FGGNSRR225 pKa = 11.84FYY227 pKa = 11.12LNTGSATAPNFAAVQTNPFGLIDD250 pKa = 3.83VGFSSAPSFADD261 pKa = 3.59LDD263 pKa = 4.06GDD265 pKa = 4.09GDD267 pKa = 4.23FDD269 pKa = 6.27LMIGEE274 pKa = 5.0DD275 pKa = 3.65NGTFNYY281 pKa = 10.17FEE283 pKa = 4.86NNGTAAAAAYY293 pKa = 7.34LAPVANPFEE302 pKa = 4.9LEE304 pKa = 4.28VTSYY308 pKa = 11.0AAPTFADD315 pKa = 3.15IDD317 pKa = 4.07ADD319 pKa = 3.89GDD321 pKa = 4.02LDD323 pKa = 4.71LFAGNADD330 pKa = 3.3GDD332 pKa = 3.97AVLFTNGGSATQPDD346 pKa = 4.67FFVTPFNLPLVGDD359 pKa = 3.95YY360 pKa = 9.11TSPALADD367 pKa = 3.87LDD369 pKa = 4.02GDD371 pKa = 3.91GDD373 pKa = 3.99VDD375 pKa = 4.84VVIGDD380 pKa = 3.88SNGTITFAEE389 pKa = 4.71NIPVGGIADD398 pKa = 3.68YY399 pKa = 9.44STNSVLVNVPAGFVIPALADD419 pKa = 3.24MDD421 pKa = 4.43GDD423 pKa = 4.12GDD425 pKa = 3.6VDD427 pKa = 3.88MLVGAGDD434 pKa = 3.53GDD436 pKa = 3.96YY437 pKa = 11.2YY438 pKa = 11.29YY439 pKa = 10.64YY440 pKa = 10.81QNNGSPTASNFVLQGTNPFGLTNLGGYY467 pKa = 9.39SGPTLVDD474 pKa = 4.1FDD476 pKa = 4.41NDD478 pKa = 3.05GDD480 pKa = 3.82VDD482 pKa = 4.08VFSGTKK488 pKa = 9.75GNGVILFEE496 pKa = 4.31NTGTASAPNFAAPVFDD512 pKa = 4.25PFGIAGVGSYY522 pKa = 9.3TRR524 pKa = 11.84TTFGDD529 pKa = 3.27VDD531 pKa = 4.13SDD533 pKa = 3.71GDD535 pKa = 3.05IDD537 pKa = 3.9MFVGQKK543 pKa = 10.62DD544 pKa = 4.4GIGLTSLSYY553 pKa = 11.38YY554 pKa = 11.24NNTTGNNTQLDD565 pKa = 4.14FAPPVDD571 pKa = 4.43FPFGIQNISTWQNPALVDD589 pKa = 3.58INNDD593 pKa = 3.0GNLDD597 pKa = 3.77LFLGQKK603 pKa = 10.43DD604 pKa = 3.75DD605 pKa = 3.54GLTRR609 pKa = 11.84VYY611 pKa = 10.45TNNKK615 pKa = 8.99LFPTISIAHH624 pKa = 6.05TGNFNEE630 pKa = 4.83CNVEE634 pKa = 4.07QIEE637 pKa = 4.45ATVGNYY643 pKa = 10.39LPDD646 pKa = 4.49DD647 pKa = 4.14VTVTWYY653 pKa = 10.69NALTNLPITTGLEE666 pKa = 4.11FNPEE670 pKa = 3.97DD671 pKa = 3.5LQFRR675 pKa = 11.84ANYY678 pKa = 8.05YY679 pKa = 10.87AVVTTTDD686 pKa = 2.77GCTDD690 pKa = 3.2QSSTFSYY697 pKa = 10.94EE698 pKa = 3.84PVTPPVGTNLSAQAGYY714 pKa = 7.99NTATLDD720 pKa = 3.29WVRR723 pKa = 11.84EE724 pKa = 4.09GDD726 pKa = 3.7CDD728 pKa = 4.33SPIRR732 pKa = 11.84EE733 pKa = 4.24YY734 pKa = 10.76EE735 pKa = 4.22VYY737 pKa = 10.82ADD739 pKa = 3.74PGQFGSYY746 pKa = 10.27FLFGYY751 pKa = 10.5SSTTNFEE758 pKa = 4.07AVGLINGEE766 pKa = 4.15KK767 pKa = 9.88INFRR771 pKa = 11.84VRR773 pKa = 11.84PIFVNGTYY781 pKa = 8.9GTYY784 pKa = 11.06SNIATVKK791 pKa = 9.26PSIVLGEE798 pKa = 4.13EE799 pKa = 4.36DD800 pKa = 3.69NEE802 pKa = 4.24KK803 pKa = 10.21TGFAFFPNPNNGEE816 pKa = 4.11FNLRR820 pKa = 11.84LQDD823 pKa = 3.56GSASATVSVTSLSGQRR839 pKa = 11.84VYY841 pKa = 7.58TTRR844 pKa = 11.84MLLKK848 pKa = 10.62LLLII852 pKa = 4.83

Molecular weight: 90.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I4ALX3|I4ALX3_BERLS Membrane protein involved in the export of O-antigen and teichoic acid OS=Bernardetia litoralis (strain ATCC 23117 / DSM 6794 / NBRC 15988 / NCIMB 1366 / Sio-4) OX=880071 GN=Fleli_2595 PE=4 SV=1
MM1 pKa = 7.59AKK3 pKa = 10.09SSMIARR9 pKa = 11.84EE10 pKa = 4.01RR11 pKa = 11.84KK12 pKa = 7.3RR13 pKa = 11.84QKK15 pKa = 10.1MVDD18 pKa = 2.93KK19 pKa = 10.27YY20 pKa = 9.07ATRR23 pKa = 11.84RR24 pKa = 11.84VEE26 pKa = 4.19LKK28 pKa = 10.64KK29 pKa = 10.39LAKK32 pKa = 10.67DD33 pKa = 3.52GDD35 pKa = 4.08VEE37 pKa = 4.46AQIALDD43 pKa = 4.31KK44 pKa = 10.88LPKK47 pKa = 10.18DD48 pKa = 3.67SSPVRR53 pKa = 11.84LHH55 pKa = 5.45NRR57 pKa = 11.84CRR59 pKa = 11.84LTGRR63 pKa = 11.84PRR65 pKa = 11.84GYY67 pKa = 8.87MRR69 pKa = 11.84RR70 pKa = 11.84FGICRR75 pKa = 11.84VVFRR79 pKa = 11.84EE80 pKa = 3.73MANDD84 pKa = 3.69GKK86 pKa = 10.81IPGVTKK92 pKa = 10.74SSWW95 pKa = 2.87

Molecular weight: 10.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3832

0

3832

1339793

29

3188

349.6

39.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.819 ± 0.035

0.773 ± 0.014

5.232 ± 0.029

7.369 ± 0.05

5.522 ± 0.03

5.62 ± 0.041

1.628 ± 0.019

8.196 ± 0.037

8.191 ± 0.072

9.289 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.019

6.312 ± 0.038

3.101 ± 0.023

3.92 ± 0.025

3.497 ± 0.024

6.652 ± 0.036

5.994 ± 0.059

5.57 ± 0.033

1.072 ± 0.012

4.159 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski