Planktothricoides sp. SR001
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5042 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M1JMA9|A0A0M1JMA9_9CYAN Uncharacterized protein OS=Planktothricoides sp. SR001 OX=1705388 GN=AM228_27290 PE=4 SV=1
MM1 pKa = 7.71 NYY3 pKa = 9.54 QNKK6 pKa = 10.18 AKK8 pKa = 9.76 IQLISGVILASTTLTIPVNAQPITPAADD36 pKa = 3.53 GTGTIVNQQDD46 pKa = 3.54 NIINITEE53 pKa = 4.6 GKK55 pKa = 9.91 RR56 pKa = 11.84 SSNGANLFHH65 pKa = 7.67 SFDD68 pKa = 3.54 QFGVNTGQTANFQSSPEE85 pKa = 3.83 INNILGRR92 pKa = 11.84 VVGGNASIINGILQVTGGNSNLFLMNPAGILFGPNASLNVPADD135 pKa = 4.05 FTATTATSIGFGNNNWFQSIGDD157 pKa = 4.01 NNWANLVGNPSDD169 pKa = 3.85 FSFDD173 pKa = 3.32 LAQPGWVVNLADD185 pKa = 3.59 LTLNFGQNLTLLGGGILNSGNLSAPGGNISIAAVPGEE222 pKa = 4.09 SLVRR226 pKa = 11.84 ISQPGNILSLDD237 pKa = 3.21 IATPGLNEE245 pKa = 4.04 GVINPLSLPEE255 pKa = 4.41 LLTGGSQILDD265 pKa = 3.4 ATQVQHH271 pKa = 6.44 NADD274 pKa = 3.41 GTITLTSSGVTIDD287 pKa = 3.95 PNTDD291 pKa = 2.81 NGLALAAGDD300 pKa = 3.74 VTTEE304 pKa = 3.53 TGGQVNVLTGGGNIILDD321 pKa = 3.6 QVNSDD326 pKa = 3.55 KK327 pKa = 11.57 VNINANGGNIAQVNSDD343 pKa = 3.65 SLLNASAVQLQTYY356 pKa = 8.03 GQGGIGLEE364 pKa = 4.23 TEE366 pKa = 4.3 PLRR369 pKa = 11.84 LEE371 pKa = 4.43 ANNLEE376 pKa = 4.2 ATAGSGGAIFYY387 pKa = 10.26 VPNGDD392 pKa = 3.51 VTIGGVTDD400 pKa = 3.91 EE401 pKa = 4.46 LTGMSITDD409 pKa = 3.53 GGDD412 pKa = 3.27 FSLEE416 pKa = 4.17 SVGSITLIEE425 pKa = 5.35 DD426 pKa = 3.98 IYY428 pKa = 11.14 TSDD431 pKa = 5.37 DD432 pKa = 3.52 IQSGDD437 pKa = 3.03 ITLTSNAGAIDD448 pKa = 3.78 TTGGGISAYY457 pKa = 10.17 SEE459 pKa = 4.17 YY460 pKa = 11.29 GNGGSVSLTAQGDD473 pKa = 3.49 IHH475 pKa = 6.45 TSNISSYY482 pKa = 10.99 SPEE485 pKa = 3.75 NGGNVSLEE493 pKa = 4.3 STGGAIDD500 pKa = 3.61 TTGGEE505 pKa = 3.84 ISANSGYY512 pKa = 11.24 GNGGSVSLTAQGDD525 pKa = 3.49 IHH527 pKa = 6.48 TSNISSDD534 pKa = 2.77 SWFWTGGNVSLEE546 pKa = 4.31 STNGAIDD553 pKa = 3.54 TTGGEE558 pKa = 3.75 ISANSVSGNAGDD570 pKa = 4.49 VSLTAQGDD578 pKa = 3.47 IHH580 pKa = 6.36 TSNIRR585 pKa = 11.84 SYY587 pKa = 11.09 SSEE590 pKa = 3.91 GNGGNVSLEE599 pKa = 4.27 STGGEE604 pKa = 3.69 IDD606 pKa = 3.55 TTGGGILAGSGSSGGDD622 pKa = 2.99 VSLIAQGDD630 pKa = 3.55 IHH632 pKa = 6.45 TSNIRR637 pKa = 11.84 SFSWDD642 pKa = 3.06 GTGGNVFLEE651 pKa = 4.52 STGGAIDD658 pKa = 3.61 TTGGEE663 pKa = 3.94 ISPYY667 pKa = 9.72 SAYY670 pKa = 10.69 GNAGDD675 pKa = 4.44 VSLIAQGDD683 pKa = 3.58 IHH685 pKa = 6.27 TSRR688 pKa = 11.84 IGSYY692 pKa = 9.99 SGEE695 pKa = 4.05 GTGGNVFLEE704 pKa = 4.52 STDD707 pKa = 3.63 GAIDD711 pKa = 3.55 TTGGEE716 pKa = 3.75 ISANSVSGNGGSVSLTAQGDD736 pKa = 3.47 IHH738 pKa = 6.36 TSNIRR743 pKa = 11.84 SYY745 pKa = 11.09 SSEE748 pKa = 3.91 GNGGNVSLEE757 pKa = 4.29 STGGAINTSAGQLDD771 pKa = 3.93 SHH773 pKa = 5.94 SQEE776 pKa = 3.88 GSAGEE781 pKa = 4.07 IKK783 pKa = 9.92 IDD785 pKa = 3.46 ASGDD789 pKa = 3.23 ILTGFVSSYY798 pKa = 9.68 NQSSSADD805 pKa = 3.75 SYY807 pKa = 12.0 SGDD810 pKa = 2.67 IWITSHH816 pKa = 6.5 NGSINTTIGNLSGEE830 pKa = 4.33 AQISPNDD837 pKa = 3.78 DD838 pKa = 3.27 VSSVEE843 pKa = 3.98 VASIFNNAQANLASYY858 pKa = 9.68 AHH860 pKa = 6.91 SGTGGNVILKK870 pKa = 10.4 ANGDD874 pKa = 3.34 ITTSHH879 pKa = 7.1 ISTFGSQSSGNVNIISNNGAINTGVIFSFSQLNNAGDD916 pKa = 3.75 VTLNAAGNISTSHH929 pKa = 5.68 ISAWGNQQGGNITIDD944 pKa = 3.22 AGNIAGQINNNTPCDD959 pKa = 3.84 CVNLLGTEE967 pKa = 4.08 PTPSFNIGSATIQSFSQVGHH987 pKa = 7.03 AGNVTITTSGDD998 pKa = 3.24 TNLGGDD1004 pKa = 4.26 EE1005 pKa = 4.04 NRR1007 pKa = 11.84 HH1008 pKa = 6.14 AIRR1011 pKa = 11.84 SAGYY1015 pKa = 8.06 TEE1017 pKa = 5.08 GGNISIFSLGG1027 pKa = 3.29
Molecular weight: 102.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.719
IPC_protein 3.757
Toseland 3.541
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.681
Rodwell 3.579
Grimsley 3.439
Solomon 3.745
Lehninger 3.706
Nozaki 3.859
DTASelect 4.101
Thurlkill 3.579
EMBOSS 3.694
Sillero 3.884
Patrickios 1.189
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A0M1JMQ7|A0A0M1JMQ7_9CYAN AAA family ATPase OS=Planktothricoides sp. SR001 OX=1705388 GN=AM228_23335 PE=4 SV=1
MM1 pKa = 7.59 GEE3 pKa = 4.18 NVNLNTGMRR12 pKa = 11.84 GPRR15 pKa = 11.84 YY16 pKa = 9.18 LWAKK20 pKa = 9.88 ILINIVGAKK29 pKa = 9.95 HH30 pKa = 6.55 SGRR33 pKa = 11.84 CFPGEE38 pKa = 4.11 NVNLITGMLRR48 pKa = 11.84 PWRR51 pKa = 11.84 YY52 pKa = 9.08 LWAKK56 pKa = 8.97 MLINIVGAKK65 pKa = 9.95 HH66 pKa = 6.55 SGRR69 pKa = 11.84 CFPGEE74 pKa = 3.89 NVNLNTGMRR83 pKa = 11.84 GPRR86 pKa = 11.84 YY87 pKa = 8.94 LWAIAMISSRR97 pKa = 11.84 CQGEE101 pKa = 3.98 AFPP104 pKa = 5.76
Molecular weight: 11.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.692
IPC_protein 10.452
Toseland 10.496
ProMoST 10.218
Dawson 10.643
Bjellqvist 10.379
Wikipedia 10.847
Rodwell 10.862
Grimsley 10.701
Solomon 10.73
Lehninger 10.687
Nozaki 10.526
DTASelect 10.35
Thurlkill 10.511
EMBOSS 10.891
Sillero 10.57
Patrickios 10.643
IPC_peptide 10.73
IPC2_peptide 9.619
IPC2.peptide.svr19 8.418
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5040
2
5042
1533545
34
2694
304.2
33.96
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.069 ± 0.036
1.008 ± 0.013
5.046 ± 0.03
6.46 ± 0.032
3.953 ± 0.022
6.727 ± 0.04
1.807 ± 0.019
6.925 ± 0.034
4.884 ± 0.028
10.877 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.989 ± 0.015
4.466 ± 0.034
4.909 ± 0.028
5.43 ± 0.033
5.072 ± 0.029
6.269 ± 0.027
5.41 ± 0.032
6.164 ± 0.032
1.465 ± 0.016
3.069 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here