Planktothricoides sp. SR001

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Planktothricoides; unclassified Planktothricoides

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5042 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0M1JMA9|A0A0M1JMA9_9CYAN Uncharacterized protein OS=Planktothricoides sp. SR001 OX=1705388 GN=AM228_27290 PE=4 SV=1
MM1 pKa = 7.71NYY3 pKa = 9.54QNKK6 pKa = 10.18AKK8 pKa = 9.76IQLISGVILASTTLTIPVNAQPITPAADD36 pKa = 3.53GTGTIVNQQDD46 pKa = 3.54NIINITEE53 pKa = 4.6GKK55 pKa = 9.91RR56 pKa = 11.84SSNGANLFHH65 pKa = 7.67SFDD68 pKa = 3.54QFGVNTGQTANFQSSPEE85 pKa = 3.83INNILGRR92 pKa = 11.84VVGGNASIINGILQVTGGNSNLFLMNPAGILFGPNASLNVPADD135 pKa = 4.05FTATTATSIGFGNNNWFQSIGDD157 pKa = 4.01NNWANLVGNPSDD169 pKa = 3.85FSFDD173 pKa = 3.32LAQPGWVVNLADD185 pKa = 3.59LTLNFGQNLTLLGGGILNSGNLSAPGGNISIAAVPGEE222 pKa = 4.09SLVRR226 pKa = 11.84ISQPGNILSLDD237 pKa = 3.21IATPGLNEE245 pKa = 4.04GVINPLSLPEE255 pKa = 4.41LLTGGSQILDD265 pKa = 3.4ATQVQHH271 pKa = 6.44NADD274 pKa = 3.41GTITLTSSGVTIDD287 pKa = 3.95PNTDD291 pKa = 2.81NGLALAAGDD300 pKa = 3.74VTTEE304 pKa = 3.53TGGQVNVLTGGGNIILDD321 pKa = 3.6QVNSDD326 pKa = 3.55KK327 pKa = 11.57VNINANGGNIAQVNSDD343 pKa = 3.65SLLNASAVQLQTYY356 pKa = 8.03GQGGIGLEE364 pKa = 4.23TEE366 pKa = 4.3PLRR369 pKa = 11.84LEE371 pKa = 4.43ANNLEE376 pKa = 4.2ATAGSGGAIFYY387 pKa = 10.26VPNGDD392 pKa = 3.51VTIGGVTDD400 pKa = 3.91EE401 pKa = 4.46LTGMSITDD409 pKa = 3.53GGDD412 pKa = 3.27FSLEE416 pKa = 4.17SVGSITLIEE425 pKa = 5.35DD426 pKa = 3.98IYY428 pKa = 11.14TSDD431 pKa = 5.37DD432 pKa = 3.52IQSGDD437 pKa = 3.03ITLTSNAGAIDD448 pKa = 3.78TTGGGISAYY457 pKa = 10.17SEE459 pKa = 4.17YY460 pKa = 11.29GNGGSVSLTAQGDD473 pKa = 3.49IHH475 pKa = 6.45TSNISSYY482 pKa = 10.99SPEE485 pKa = 3.75NGGNVSLEE493 pKa = 4.3STGGAIDD500 pKa = 3.61TTGGEE505 pKa = 3.84ISANSGYY512 pKa = 11.24GNGGSVSLTAQGDD525 pKa = 3.49IHH527 pKa = 6.48TSNISSDD534 pKa = 2.77SWFWTGGNVSLEE546 pKa = 4.31STNGAIDD553 pKa = 3.54TTGGEE558 pKa = 3.75ISANSVSGNAGDD570 pKa = 4.49VSLTAQGDD578 pKa = 3.47IHH580 pKa = 6.36TSNIRR585 pKa = 11.84SYY587 pKa = 11.09SSEE590 pKa = 3.91GNGGNVSLEE599 pKa = 4.27STGGEE604 pKa = 3.69IDD606 pKa = 3.55TTGGGILAGSGSSGGDD622 pKa = 2.99VSLIAQGDD630 pKa = 3.55IHH632 pKa = 6.45TSNIRR637 pKa = 11.84SFSWDD642 pKa = 3.06GTGGNVFLEE651 pKa = 4.52STGGAIDD658 pKa = 3.61TTGGEE663 pKa = 3.94ISPYY667 pKa = 9.72SAYY670 pKa = 10.69GNAGDD675 pKa = 4.44VSLIAQGDD683 pKa = 3.58IHH685 pKa = 6.27TSRR688 pKa = 11.84IGSYY692 pKa = 9.99SGEE695 pKa = 4.05GTGGNVFLEE704 pKa = 4.52STDD707 pKa = 3.63GAIDD711 pKa = 3.55TTGGEE716 pKa = 3.75ISANSVSGNGGSVSLTAQGDD736 pKa = 3.47IHH738 pKa = 6.36TSNIRR743 pKa = 11.84SYY745 pKa = 11.09SSEE748 pKa = 3.91GNGGNVSLEE757 pKa = 4.29STGGAINTSAGQLDD771 pKa = 3.93SHH773 pKa = 5.94SQEE776 pKa = 3.88GSAGEE781 pKa = 4.07IKK783 pKa = 9.92IDD785 pKa = 3.46ASGDD789 pKa = 3.23ILTGFVSSYY798 pKa = 9.68NQSSSADD805 pKa = 3.75SYY807 pKa = 12.0SGDD810 pKa = 2.67IWITSHH816 pKa = 6.5NGSINTTIGNLSGEE830 pKa = 4.33AQISPNDD837 pKa = 3.78DD838 pKa = 3.27VSSVEE843 pKa = 3.98VASIFNNAQANLASYY858 pKa = 9.68AHH860 pKa = 6.91SGTGGNVILKK870 pKa = 10.4ANGDD874 pKa = 3.34ITTSHH879 pKa = 7.1ISTFGSQSSGNVNIISNNGAINTGVIFSFSQLNNAGDD916 pKa = 3.75VTLNAAGNISTSHH929 pKa = 5.68ISAWGNQQGGNITIDD944 pKa = 3.22AGNIAGQINNNTPCDD959 pKa = 3.84CVNLLGTEE967 pKa = 4.08PTPSFNIGSATIQSFSQVGHH987 pKa = 7.03AGNVTITTSGDD998 pKa = 3.24TNLGGDD1004 pKa = 4.26EE1005 pKa = 4.04NRR1007 pKa = 11.84HH1008 pKa = 6.14AIRR1011 pKa = 11.84SAGYY1015 pKa = 8.06TEE1017 pKa = 5.08GGNISIFSLGG1027 pKa = 3.29

Molecular weight:
102.96 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0M1JMQ7|A0A0M1JMQ7_9CYAN AAA family ATPase OS=Planktothricoides sp. SR001 OX=1705388 GN=AM228_23335 PE=4 SV=1
MM1 pKa = 7.59GEE3 pKa = 4.18NVNLNTGMRR12 pKa = 11.84GPRR15 pKa = 11.84YY16 pKa = 9.18LWAKK20 pKa = 9.88ILINIVGAKK29 pKa = 9.95HH30 pKa = 6.55SGRR33 pKa = 11.84CFPGEE38 pKa = 4.11NVNLITGMLRR48 pKa = 11.84PWRR51 pKa = 11.84YY52 pKa = 9.08LWAKK56 pKa = 8.97MLINIVGAKK65 pKa = 9.95HH66 pKa = 6.55SGRR69 pKa = 11.84CFPGEE74 pKa = 3.89NVNLNTGMRR83 pKa = 11.84GPRR86 pKa = 11.84YY87 pKa = 8.94LWAIAMISSRR97 pKa = 11.84CQGEE101 pKa = 3.98AFPP104 pKa = 5.76

Molecular weight:
11.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5040

2

5042

1533545

34

2694

304.2

33.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.069 ± 0.036

1.008 ± 0.013

5.046 ± 0.03

6.46 ± 0.032

3.953 ± 0.022

6.727 ± 0.04

1.807 ± 0.019

6.925 ± 0.034

4.884 ± 0.028

10.877 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.989 ± 0.015

4.466 ± 0.034

4.909 ± 0.028

5.43 ± 0.033

5.072 ± 0.029

6.269 ± 0.027

5.41 ± 0.032

6.164 ± 0.032

1.465 ± 0.016

3.069 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski