Amino acid dipepetide frequency for Planktothricoides sp. SR001

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.512AlaAla: 7.512 ± 0.097
0.791AlaCys: 0.791 ± 0.024
4.329AlaAsp: 4.329 ± 0.067
5.907AlaGlu: 5.907 ± 0.072
2.78AlaPhe: 2.78 ± 0.049
5.426AlaGly: 5.426 ± 0.075
1.221AlaHis: 1.221 ± 0.031
7.548AlaIle: 7.548 ± 0.081
4.395AlaLys: 4.395 ± 0.059
7.873AlaLeu: 7.873 ± 0.086
1.684AlaMet: 1.684 ± 0.039
3.683AlaAsn: 3.683 ± 0.079
2.88AlaPro: 2.88 ± 0.047
4.017AlaGln: 4.017 ± 0.059
3.332AlaArg: 3.332 ± 0.054
4.427AlaSer: 4.427 ± 0.065
4.475AlaThr: 4.475 ± 0.068
5.038AlaVal: 5.038 ± 0.062
1.002AlaTrp: 1.002 ± 0.032
2.372AlaTyr: 2.372 ± 0.042
0.001AlaXaa: 0.001 ± 0.001
Cys
0.639CysAla: 0.639 ± 0.021
0.173CysCys: 0.173 ± 0.011
0.621CysAsp: 0.621 ± 0.023
0.52CysGlu: 0.52 ± 0.017
0.428CysPhe: 0.428 ± 0.016
0.807CysGly: 0.807 ± 0.024
0.313CysHis: 0.313 ± 0.013
0.558CysIle: 0.558 ± 0.02
0.284CysLys: 0.284 ± 0.013
1.257CysLeu: 1.257 ± 0.03
0.156CysMet: 0.156 ± 0.01
0.341CysAsn: 0.341 ± 0.014
0.584CysPro: 0.584 ± 0.023
0.703CysGln: 0.703 ± 0.023
0.573CysArg: 0.573 ± 0.021
0.588CysSer: 0.588 ± 0.02
0.455CysThr: 0.455 ± 0.017
0.537CysVal: 0.537 ± 0.021
0.168CysTrp: 0.168 ± 0.01
0.372CysTyr: 0.372 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
3.626AspAla: 3.626 ± 0.05
0.528AspCys: 0.528 ± 0.019
2.379AspAsp: 2.379 ± 0.055
3.013AspGlu: 3.013 ± 0.05
2.366AspPhe: 2.366 ± 0.041
3.261AspGly: 3.261 ± 0.065
0.833AspHis: 0.833 ± 0.033
3.544AspIle: 3.544 ± 0.061
1.975AspLys: 1.975 ± 0.035
6.206AspLeu: 6.206 ± 0.078
0.893AspMet: 0.893 ± 0.023
1.946AspAsn: 1.946 ± 0.04
2.837AspPro: 2.837 ± 0.047
2.061AspGln: 2.061 ± 0.044
4.474AspArg: 4.474 ± 0.052
2.82AspSer: 2.82 ± 0.046
2.117AspThr: 2.117 ± 0.037
2.646AspVal: 2.646 ± 0.037
0.932AspTrp: 0.932 ± 0.026
2.003AspTyr: 2.003 ± 0.04
0.0AspXaa: 0.0 ± 0.0
Glu
5.65GluAla: 5.65 ± 0.073
0.462GluCys: 0.462 ± 0.018
2.72GluAsp: 2.72 ± 0.044
4.124GluGlu: 4.124 ± 0.07
2.628GluPhe: 2.628 ± 0.045
3.054GluGly: 3.054 ± 0.056
0.943GluHis: 0.943 ± 0.027
5.499GluIle: 5.499 ± 0.057
4.12GluLys: 4.12 ± 0.059
7.112GluLeu: 7.112 ± 0.079
1.523GluMet: 1.523 ± 0.036
3.293GluAsn: 3.293 ± 0.058
2.546GluPro: 2.546 ± 0.038
3.451GluGln: 3.451 ± 0.06
3.07GluArg: 3.07 ± 0.055
3.562GluSer: 3.562 ± 0.048
3.989GluThr: 3.989 ± 0.059
4.061GluVal: 4.061 ± 0.05
0.829GluTrp: 0.829 ± 0.022
1.966GluTyr: 1.966 ± 0.037
0.0GluXaa: 0.0 ± 0.0
Phe
3.205PheAla: 3.205 ± 0.048
0.58PheCys: 0.58 ± 0.019
2.201PheAsp: 2.201 ± 0.036
2.094PheGlu: 2.094 ± 0.035
1.822PhePhe: 1.822 ± 0.039
2.882PheGly: 2.882 ± 0.046
0.753PheHis: 0.753 ± 0.022
2.405PheIle: 2.405 ± 0.047
1.523PheLys: 1.523 ± 0.032
4.294PheLeu: 4.294 ± 0.07
0.771PheMet: 0.771 ± 0.023
1.824PheAsn: 1.824 ± 0.036
1.849PhePro: 1.849 ± 0.037
1.841PheGln: 1.841 ± 0.035
1.811PheArg: 1.811 ± 0.035
2.97PheSer: 2.97 ± 0.05
2.263PheThr: 2.263 ± 0.042
2.266PheVal: 2.266 ± 0.038
0.728PheTrp: 0.728 ± 0.025
1.448PheTyr: 1.448 ± 0.035
0.0PheXaa: 0.0 ± 0.0
Gly
4.837GlyAla: 4.837 ± 0.091
0.816GlyCys: 0.816 ± 0.028
3.69GlyAsp: 3.69 ± 0.076
4.329GlyGlu: 4.329 ± 0.057
3.043GlyPhe: 3.043 ± 0.053
4.864GlyGly: 4.864 ± 0.112
1.307GlyHis: 1.307 ± 0.045
5.02GlyIle: 5.02 ± 0.065
4.044GlyLys: 4.044 ± 0.065
6.986GlyLeu: 6.986 ± 0.081
1.607GlyMet: 1.607 ± 0.037
3.251GlyAsn: 3.251 ± 0.099
1.374GlyPro: 1.374 ± 0.036
3.347GlyGln: 3.347 ± 0.054
3.093GlyArg: 3.093 ± 0.051
4.248GlySer: 4.248 ± 0.065
3.553GlyThr: 3.553 ± 0.065
4.404GlyVal: 4.404 ± 0.056
1.126GlyTrp: 1.126 ± 0.028
2.326GlyTyr: 2.326 ± 0.042
0.001GlyXaa: 0.001 ± 0.001
His
1.014HisAla: 1.014 ± 0.024
0.268HisCys: 0.268 ± 0.013
0.741HisAsp: 0.741 ± 0.029
0.862HisGlu: 0.862 ± 0.022
0.755HisPhe: 0.755 ± 0.025
1.124HisGly: 1.124 ± 0.027
0.645HisHis: 0.645 ± 0.027
1.109HisIle: 1.109 ± 0.029
0.763HisLys: 0.763 ± 0.022
2.244HisLeu: 2.244 ± 0.051
0.207HisMet: 0.207 ± 0.011
0.726HisAsn: 0.726 ± 0.02
1.409HisPro: 1.409 ± 0.035
1.315HisGln: 1.315 ± 0.029
1.13HisArg: 1.13 ± 0.027
1.157HisSer: 1.157 ± 0.033
0.848HisThr: 0.848 ± 0.028
0.694HisVal: 0.694 ± 0.027
0.363HisTrp: 0.363 ± 0.015
0.7HisTyr: 0.7 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.507IleAla: 7.507 ± 0.091
0.863IleCys: 0.863 ± 0.022
3.808IleAsp: 3.808 ± 0.054
4.181IleGlu: 4.181 ± 0.058
2.87IlePhe: 2.87 ± 0.05
4.607IleGly: 4.607 ± 0.066
1.288IleHis: 1.288 ± 0.03
3.926IleIle: 3.926 ± 0.059
3.198IleLys: 3.198 ± 0.056
7.093IleLeu: 7.093 ± 0.069
0.994IleMet: 0.994 ± 0.025
3.148IleAsn: 3.148 ± 0.059
3.66IlePro: 3.66 ± 0.047
3.214IleGln: 3.214 ± 0.048
3.229IleArg: 3.229 ± 0.048
4.872IleSer: 4.872 ± 0.061
3.715IleThr: 3.715 ± 0.058
4.035IleVal: 4.035 ± 0.057
0.919IleTrp: 0.919 ± 0.029
2.127IleTyr: 2.127 ± 0.035
0.0IleXaa: 0.0 ± 0.0
Lys
3.8LysAla: 3.8 ± 0.061
0.345LysCys: 0.345 ± 0.014
2.098LysAsp: 2.098 ± 0.043
2.859LysGlu: 2.859 ± 0.051
1.973LysPhe: 1.973 ± 0.041
2.585LysGly: 2.585 ± 0.049
0.818LysHis: 0.818 ± 0.025
4.063LysIle: 4.063 ± 0.053
2.746LysLys: 2.746 ± 0.055
5.389LysLeu: 5.389 ± 0.07
1.089LysMet: 1.089 ± 0.023
2.49LysAsn: 2.49 ± 0.038
2.486LysPro: 2.486 ± 0.043
2.641LysGln: 2.641 ± 0.046
2.24LysArg: 2.24 ± 0.043
3.15LysSer: 3.15 ± 0.048
3.095LysThr: 3.095 ± 0.052
2.921LysVal: 2.921 ± 0.045
0.503LysTrp: 0.503 ± 0.017
1.549LysTyr: 1.549 ± 0.034
0.0LysXaa: 0.0 ± 0.0
Leu
9.419LeuAla: 9.419 ± 0.085
1.02LeuCys: 1.02 ± 0.026
5.497LeuAsp: 5.497 ± 0.067
7.431LeuGlu: 7.431 ± 0.089
3.84LeuPhe: 3.84 ± 0.058
7.618LeuGly: 7.618 ± 0.085
1.757LeuHis: 1.757 ± 0.033
6.907LeuIle: 6.907 ± 0.082
5.691LeuLys: 5.691 ± 0.068
10.856LeuLeu: 10.856 ± 0.121
2.381LeuMet: 2.381 ± 0.043
4.838LeuAsn: 4.838 ± 0.059
5.629LeuPro: 5.629 ± 0.072
5.202LeuGln: 5.202 ± 0.062
5.448LeuArg: 5.448 ± 0.074
7.633LeuSer: 7.633 ± 0.09
6.451LeuThr: 6.451 ± 0.076
6.799LeuVal: 6.799 ± 0.078
1.584LeuTrp: 1.584 ± 0.037
2.775LeuTyr: 2.775 ± 0.05
0.0LeuXaa: 0.0 ± 0.0
Met
1.873MetAla: 1.873 ± 0.034
0.125MetCys: 0.125 ± 0.01
0.864MetAsp: 0.864 ± 0.023
1.053MetGlu: 1.053 ± 0.029
0.606MetPhe: 0.606 ± 0.022
1.473MetGly: 1.473 ± 0.036
0.28MetHis: 0.28 ± 0.015
1.317MetIle: 1.317 ± 0.03
1.056MetLys: 1.056 ± 0.028
1.942MetLeu: 1.942 ± 0.046
0.508MetMet: 0.508 ± 0.021
0.978MetAsn: 0.978 ± 0.027
1.047MetPro: 1.047 ± 0.028
0.981MetGln: 0.981 ± 0.026
1.015MetArg: 1.015 ± 0.025
1.424MetSer: 1.424 ± 0.03
1.403MetThr: 1.403 ± 0.028
1.423MetVal: 1.423 ± 0.035
0.15MetTrp: 0.15 ± 0.01
0.366MetTyr: 0.366 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.991AsnAla: 2.991 ± 0.052
0.482AsnCys: 0.482 ± 0.019
1.794AsnAsp: 1.794 ± 0.045
1.82AsnGlu: 1.82 ± 0.038
1.976AsnPhe: 1.976 ± 0.042
2.719AsnGly: 2.719 ± 0.07
0.992AsnHis: 0.992 ± 0.03
2.975AsnIle: 2.975 ± 0.068
1.538AsnLys: 1.538 ± 0.036
6.043AsnLeu: 6.043 ± 0.097
0.741AsnMet: 0.741 ± 0.022
2.018AsnAsn: 2.018 ± 0.062
3.228AsnPro: 3.228 ± 0.05
3.231AsnGln: 3.231 ± 0.057
2.441AsnArg: 2.441 ± 0.042
3.122AsnSer: 3.122 ± 0.063
2.056AsnThr: 2.056 ± 0.044
2.113AsnVal: 2.113 ± 0.044
0.781AsnTrp: 0.781 ± 0.022
1.596AsnTyr: 1.596 ± 0.035
0.0AsnXaa: 0.0 ± 0.0
Pro
3.318ProAla: 3.318 ± 0.046
0.383ProCys: 0.383 ± 0.016
3.22ProAsp: 3.22 ± 0.05
4.588ProGlu: 4.588 ± 0.05
1.632ProPhe: 1.632 ± 0.03
3.512ProGly: 3.512 ± 0.058
0.843ProHis: 0.843 ± 0.026
3.056ProIle: 3.056 ± 0.05
2.349ProLys: 2.349 ± 0.041
4.677ProLeu: 4.677 ± 0.063
0.891ProMet: 0.891 ± 0.024
2.423ProAsn: 2.423 ± 0.048
2.656ProPro: 2.656 ± 0.053
2.584ProGln: 2.584 ± 0.039
1.873ProArg: 1.873 ± 0.037
2.764ProSer: 2.764 ± 0.047
2.794ProThr: 2.794 ± 0.064
3.508ProVal: 3.508 ± 0.056
0.672ProTrp: 0.672 ± 0.023
1.346ProTyr: 1.346 ± 0.029
0.001ProXaa: 0.001 ± 0.001
Gln
4.716GlnAla: 4.716 ± 0.069
0.391GlnCys: 0.391 ± 0.015
2.193GlnAsp: 2.193 ± 0.042
3.817GlnGlu: 3.817 ± 0.048
1.842GlnPhe: 1.842 ± 0.039
3.465GlnGly: 3.465 ± 0.05
0.811GlnHis: 0.811 ± 0.023
3.952GlnIle: 3.952 ± 0.063
3.183GlnLys: 3.183 ± 0.052
5.865GlnLeu: 5.865 ± 0.083
1.073GlnMet: 1.073 ± 0.029
2.292GlnAsn: 2.292 ± 0.046
2.765GlnPro: 2.765 ± 0.051
3.495GlnGln: 3.495 ± 0.067
2.821GlnArg: 2.821 ± 0.041
2.841GlnSer: 2.841 ± 0.053
2.954GlnThr: 2.954 ± 0.052
3.699GlnVal: 3.699 ± 0.048
0.813GlnTrp: 0.813 ± 0.023
1.314GlnTyr: 1.314 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
3.082ArgAla: 3.082 ± 0.056
0.564ArgCys: 0.564 ± 0.018
2.755ArgAsp: 2.755 ± 0.045
3.586ArgGlu: 3.586 ± 0.051
2.179ArgPhe: 2.179 ± 0.039
3.172ArgGly: 3.172 ± 0.056
1.009ArgHis: 1.009 ± 0.027
3.294ArgIle: 3.294 ± 0.051
2.186ArgLys: 2.186 ± 0.044
5.83ArgLeu: 5.83 ± 0.068
1.071ArgMet: 1.071 ± 0.027
2.033ArgAsn: 2.033 ± 0.04
2.17ArgPro: 2.17 ± 0.038
3.423ArgGln: 3.423 ± 0.054
2.895ArgArg: 2.895 ± 0.056
3.36ArgSer: 3.36 ± 0.05
2.256ArgThr: 2.256 ± 0.037
3.197ArgVal: 3.197 ± 0.055
0.801ArgTrp: 0.801 ± 0.023
1.858ArgTyr: 1.858 ± 0.037
0.0ArgXaa: 0.0 ± 0.0
Ser
4.663SerAla: 4.663 ± 0.058
0.621SerCys: 0.621 ± 0.02
3.445SerAsp: 3.445 ± 0.056
3.991SerGlu: 3.991 ± 0.053
2.366SerPhe: 2.366 ± 0.042
4.918SerGly: 4.918 ± 0.076
1.275SerHis: 1.275 ± 0.033
3.559SerIle: 3.559 ± 0.047
2.46SerLys: 2.46 ± 0.042
7.356SerLeu: 7.356 ± 0.083
1.205SerMet: 1.205 ± 0.028
2.567SerAsn: 2.567 ± 0.047
3.905SerPro: 3.905 ± 0.063
3.796SerGln: 3.796 ± 0.057
3.124SerArg: 3.124 ± 0.044
4.062SerSer: 4.062 ± 0.069
3.056SerThr: 3.056 ± 0.056
3.62SerVal: 3.62 ± 0.049
0.929SerTrp: 0.929 ± 0.024
1.766SerTyr: 1.766 ± 0.038
0.0SerXaa: 0.0 ± 0.0
Thr
4.449ThrAla: 4.449 ± 0.061
0.481ThrCys: 0.481 ± 0.019
2.635ThrAsp: 2.635 ± 0.044
3.583ThrGlu: 3.583 ± 0.056
1.955ThrPhe: 1.955 ± 0.04
4.458ThrGly: 4.458 ± 0.072
0.926ThrHis: 0.926 ± 0.023
3.813ThrIle: 3.813 ± 0.066
2.187ThrLys: 2.187 ± 0.043
6.066ThrLeu: 6.066 ± 0.067
0.893ThrMet: 0.893 ± 0.022
2.14ThrAsn: 2.14 ± 0.044
3.296ThrPro: 3.296 ± 0.051
2.672ThrGln: 2.672 ± 0.045
2.278ThrArg: 2.278 ± 0.038
3.047ThrSer: 3.047 ± 0.049
3.067ThrThr: 3.067 ± 0.062
3.879ThrVal: 3.879 ± 0.064
0.732ThrTrp: 0.732 ± 0.023
1.547ThrTyr: 1.547 ± 0.033
0.0ThrXaa: 0.0 ± 0.0
Val
5.395ValAla: 5.395 ± 0.066
0.642ValCys: 0.642 ± 0.02
3.21ValAsp: 3.21 ± 0.055
4.128ValGlu: 4.128 ± 0.055
2.46ValPhe: 2.46 ± 0.043
4.271ValGly: 4.271 ± 0.061
0.96ValHis: 0.96 ± 0.027
4.087ValIle: 4.087 ± 0.06
3.371ValLys: 3.371 ± 0.052
6.045ValLeu: 6.045 ± 0.072
1.422ValMet: 1.422 ± 0.03
2.906ValAsn: 2.906 ± 0.05
2.628ValPro: 2.628 ± 0.039
2.636ValGln: 2.636 ± 0.043
3.0ValArg: 3.0 ± 0.055
3.981ValSer: 3.981 ± 0.044
3.644ValThr: 3.644 ± 0.057
4.295ValVal: 4.295 ± 0.067
0.814ValTrp: 0.814 ± 0.025
1.746ValTyr: 1.746 ± 0.034
0.0ValXaa: 0.0 ± 0.0
Trp
0.942TrpAla: 0.942 ± 0.029
0.143TrpCys: 0.143 ± 0.011
0.711TrpAsp: 0.711 ± 0.03
0.97TrpGlu: 0.97 ± 0.026
0.608TrpPhe: 0.608 ± 0.021
0.963TrpGly: 0.963 ± 0.026
0.334TrpHis: 0.334 ± 0.016
0.856TrpIle: 0.856 ± 0.024
0.596TrpLys: 0.596 ± 0.021
1.95TrpLeu: 1.95 ± 0.046
0.339TrpMet: 0.339 ± 0.017
0.552TrpAsn: 0.552 ± 0.021
0.481TrpPro: 0.481 ± 0.019
1.352TrpGln: 1.352 ± 0.033
0.839TrpArg: 0.839 ± 0.024
0.8TrpSer: 0.8 ± 0.024
0.579TrpThr: 0.579 ± 0.021
0.972TrpVal: 0.972 ± 0.026
0.247TrpTrp: 0.247 ± 0.014
0.419TrpTyr: 0.419 ± 0.017
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.053TyrAla: 2.053 ± 0.04
0.402TyrCys: 0.402 ± 0.014
1.547TyrAsp: 1.547 ± 0.034
1.748TyrGlu: 1.748 ± 0.041
1.397TyrPhe: 1.397 ± 0.03
2.088TyrGly: 2.088 ± 0.035
0.765TyrHis: 0.765 ± 0.021
1.765TyrIle: 1.765 ± 0.032
1.176TyrLys: 1.176 ± 0.028
3.689TyrLeu: 3.689 ± 0.051
0.437TyrMet: 0.437 ± 0.018
1.206TyrAsn: 1.206 ± 0.031
1.662TyrPro: 1.662 ± 0.032
2.34TyrGln: 2.34 ± 0.044
2.033TyrArg: 2.033 ± 0.04
1.861TyrSer: 1.861 ± 0.035
1.332TyrThr: 1.332 ± 0.031
1.534TyrVal: 1.534 ± 0.033
0.562TyrTrp: 0.562 ± 0.023
1.094TyrTyr: 1.094 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.001XaaAsp: 0.001 ± 0.001
0.0XaaGlu: 0.0 ± 0.0
0.001XaaPhe: 0.001 ± 0.001
0.001XaaGly: 0.001 ± 0.001
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.014XaaXaa: 0.014 ± 0.009
Statistics based on 5042 proteins (1533545 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski