Lactobacillus hayakitensis DSM 18933 = JCM 14209

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Ligilactobacillus; Ligilactobacillus hayakitensis

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1536 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0R1WNH9|A0A0R1WNH9_9LACO Threonine--tRNA ligase OS=Lactobacillus hayakitensis DSM 18933 = JCM 14209 OX=1423755 GN=thrS PE=3 SV=1
MM1 pKa = 7.95EE2 pKa = 3.97ITIFSNEE9 pKa = 3.73DD10 pKa = 3.06VVRR13 pKa = 11.84EE14 pKa = 3.94KK15 pKa = 11.1FQGKK19 pKa = 6.31WVNISFLDD27 pKa = 3.45NKK29 pKa = 10.74SPFRR33 pKa = 11.84VFVVSVDD40 pKa = 3.32NDD42 pKa = 3.89YY43 pKa = 11.79EE44 pKa = 4.64DD45 pKa = 5.31DD46 pKa = 4.06NYY48 pKa = 11.44AGSAIYY54 pKa = 10.5YY55 pKa = 9.11NNSGANDD62 pKa = 3.79YY63 pKa = 11.34ADD65 pKa = 3.63EE66 pKa = 4.77ALPFYY71 pKa = 10.23QIQSIEE77 pKa = 4.07LSSTQNDD84 pKa = 3.29NN85 pKa = 3.13

Molecular weight: 9.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0R1WV91|A0A0R1WV91_9LACO Glutamine-binding protein glutamine transport system permease protein OS=Lactobacillus hayakitensis DSM 18933 = JCM 14209 OX=1423755 GN=FC40_GL001239 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.69QPKK8 pKa = 8.69KK9 pKa = 7.77RR10 pKa = 11.84HH11 pKa = 4.99RR12 pKa = 11.84QRR14 pKa = 11.84VHH16 pKa = 6.07GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTSNGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1536

0

1536

470751

44

3659

306.5

34.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.99 ± 0.075

0.451 ± 0.015

5.813 ± 0.061

6.648 ± 0.081

4.383 ± 0.057

6.266 ± 0.057

1.704 ± 0.023

7.601 ± 0.077

8.097 ± 0.064

9.288 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.67 ± 0.031

5.692 ± 0.064

3.213 ± 0.046

4.35 ± 0.062

3.598 ± 0.053

5.977 ± 0.081

5.641 ± 0.077

6.945 ± 0.057

0.858 ± 0.021

3.787 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski