Comamonadaceae bacterium NML00-0135

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; unclassified Comamonadaceae

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2590 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A2A831|A0A2A2A831_9BURK Uncharacterized protein OS=Comamonadaceae bacterium NML00-0135 OX=2029116 GN=CK625_13280 PE=4 SV=1
MM1 pKa = 7.84PAPIHH6 pKa = 5.55FTDD9 pKa = 3.65AAAAKK14 pKa = 9.92VADD17 pKa = 5.47LIAEE21 pKa = 4.31EE22 pKa = 4.82GNPDD26 pKa = 2.99LKK28 pKa = 11.13LRR30 pKa = 11.84VFVQGGGCSGFQYY43 pKa = 10.94GFTFDD48 pKa = 4.81EE49 pKa = 4.64ITNEE53 pKa = 4.77DD54 pKa = 4.2DD55 pKa = 3.25TTMTKK60 pKa = 10.66NGVSLLIDD68 pKa = 3.54AMSYY72 pKa = 10.57QYY74 pKa = 11.72LLGAEE79 pKa = 3.98IDD81 pKa = 4.02YY82 pKa = 11.34KK83 pKa = 11.22EE84 pKa = 4.67DD85 pKa = 3.23LQGAQFVIKK94 pKa = 10.4NPNATTTCGCGSSFSVV110 pKa = 3.54

Molecular weight: 11.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A2AGQ8|A0A2A2AGQ8_9BURK Sulfur reduction protein DsrE OS=Comamonadaceae bacterium NML00-0135 OX=2029116 GN=CK625_09870 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.07QPSKK9 pKa = 9.07IRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.44GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LGLSS45 pKa = 3.08

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2590

0

2590

887569

37

2642

342.7

37.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.922 ± 0.086

0.949 ± 0.015

4.66 ± 0.031

4.923 ± 0.047

3.133 ± 0.032

8.16 ± 0.045

2.513 ± 0.026

4.192 ± 0.039

2.852 ± 0.047

11.348 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.023

2.398 ± 0.03

5.728 ± 0.044

6.146 ± 0.061

6.803 ± 0.046

5.09 ± 0.031

4.496 ± 0.038

6.504 ± 0.049

1.638 ± 0.025

2.224 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski