Amino acid dipepetide frequency for Comamonadaceae bacterium NML00-0135

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.815AlaAla: 20.815 ± 0.244	1.465AlaCys: 1.465 ± 0.042	5.803AlaAsp: 5.803 ± 0.098	6.231AlaGlu: 6.231 ± 0.094	3.601AlaPhe: 3.601 ± 0.062	10.467AlaGly: 10.467 ± 0.151	3.348AlaHis: 3.348 ± 0.07	5.327AlaIle: 5.327 ± 0.079	3.533AlaLys: 3.533 ± 0.096	18.181AlaLeu: 18.181 ± 0.226	3.484AlaMet: 3.484 ± 0.069	2.767AlaAsn: 2.767 ± 0.069	8.103AlaPro: 8.103 ± 0.146	11.159AlaGln: 11.159 ± 0.181	9.411AlaArg: 9.411 ± 0.129	7.185AlaSer: 7.185 ± 0.1	5.689AlaThr: 5.689 ± 0.109	7.96AlaVal: 7.96 ± 0.115	2.266AlaTrp: 2.266 ± 0.065	2.427AlaTyr: 2.427 ± 0.05	0.0AlaXaa: 0.0 ± 0.0
Cys	1.352CysAla: 1.352 ± 0.048	0.142CysCys: 0.142 ± 0.014	0.518CysAsp: 0.518 ± 0.024	0.465CysGlu: 0.465 ± 0.025	0.279CysPhe: 0.279 ± 0.019	0.899CysGly: 0.899 ± 0.033	0.243CysHis: 0.243 ± 0.017	0.433CysIle: 0.433 ± 0.021	0.197CysLys: 0.197 ± 0.015	0.937CysLeu: 0.937 ± 0.035	0.254CysMet: 0.254 ± 0.017	0.223CysAsn: 0.223 ± 0.019	0.543CysPro: 0.543 ± 0.029	0.453CysGln: 0.453 ± 0.025	0.533CysArg: 0.533 ± 0.025	0.555CysSer: 0.555 ± 0.025	0.517CysThr: 0.517 ± 0.029	0.62CysVal: 0.62 ± 0.027	0.149CysTrp: 0.149 ± 0.014	0.18CysTyr: 0.18 ± 0.014	0.0CysXaa: 0.0 ± 0.0
Asp	7.437AspAla: 7.437 ± 0.113	0.472AspCys: 0.472 ± 0.024	2.138AspAsp: 2.138 ± 0.065	2.91AspGlu: 2.91 ± 0.072	1.919AspPhe: 1.919 ± 0.05	4.206AspGly: 4.206 ± 0.086	0.891AspHis: 0.891 ± 0.036	2.17AspIle: 2.17 ± 0.053	1.546AspLys: 1.546 ± 0.052	4.37AspLeu: 4.37 ± 0.079	1.176AspMet: 1.176 ± 0.037	1.053AspAsn: 1.053 ± 0.045	2.387AspPro: 2.387 ± 0.06	1.509AspGln: 1.509 ± 0.042	2.293AspArg: 2.293 ± 0.057	2.185AspSer: 2.185 ± 0.053	2.111AspThr: 2.111 ± 0.049	3.377AspVal: 3.377 ± 0.076	1.04AspTrp: 1.04 ± 0.034	1.413AspTyr: 1.413 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	6.176GluAla: 6.176 ± 0.11	0.326GluCys: 0.326 ± 0.021	2.098GluAsp: 2.098 ± 0.056	2.273GluGlu: 2.273 ± 0.065	1.524GluPhe: 1.524 ± 0.045	3.493GluGly: 3.493 ± 0.076	1.521GluHis: 1.521 ± 0.051	2.387GluIle: 2.387 ± 0.067	1.738GluLys: 1.738 ± 0.066	5.823GluLeu: 5.823 ± 0.096	1.051GluMet: 1.051 ± 0.037	1.139GluAsn: 1.139 ± 0.039	2.624GluPro: 2.624 ± 0.055	3.739GluGln: 3.739 ± 0.072	4.438GluArg: 4.438 ± 0.068	1.957GluSer: 1.957 ± 0.051	1.93GluThr: 1.93 ± 0.05	3.326GluVal: 3.326 ± 0.07	0.581GluTrp: 0.581 ± 0.03	1.087GluTyr: 1.087 ± 0.037	0.0GluXaa: 0.0 ± 0.0
Phe	4.154PheAla: 4.154 ± 0.076	0.375PheCys: 0.375 ± 0.02	2.173PheAsp: 2.173 ± 0.059	2.014PheGlu: 2.014 ± 0.049	1.304PhePhe: 1.304 ± 0.039	2.825PheGly: 2.825 ± 0.064	0.677PheHis: 0.677 ± 0.025	1.494PheIle: 1.494 ± 0.041	0.989PheLys: 0.989 ± 0.042	2.716PheLeu: 2.716 ± 0.06	0.782PheMet: 0.782 ± 0.031	0.997PheAsn: 0.997 ± 0.036	1.237PhePro: 1.237 ± 0.039	1.044PheGln: 1.044 ± 0.035	1.483PheArg: 1.483 ± 0.041	1.896PheSer: 1.896 ± 0.056	1.625PheThr: 1.625 ± 0.041	2.221PheVal: 2.221 ± 0.061	0.504PheTrp: 0.504 ± 0.026	0.817PheTyr: 0.817 ± 0.033	0.0PheXaa: 0.0 ± 0.0
Gly	9.58GlyAla: 9.58 ± 0.116	0.871GlyCys: 0.871 ± 0.034	3.533GlyAsp: 3.533 ± 0.076	3.804GlyGlu: 3.804 ± 0.077	2.973GlyPhe: 2.973 ± 0.06	6.548GlyGly: 6.548 ± 0.117	2.099GlyHis: 2.099 ± 0.048	3.763GlyIle: 3.763 ± 0.071	3.093GlyLys: 3.093 ± 0.07	9.196GlyLeu: 9.196 ± 0.134	2.259GlyMet: 2.259 ± 0.056	2.236GlyAsn: 2.236 ± 0.068	3.33GlyPro: 3.33 ± 0.07	5.676GlyGln: 5.676 ± 0.095	5.397GlyArg: 5.397 ± 0.087	4.458GlySer: 4.458 ± 0.084	3.59GlyThr: 3.59 ± 0.075	5.562GlyVal: 5.562 ± 0.091	1.493GlyTrp: 1.493 ± 0.041	2.134GlyTyr: 2.134 ± 0.047	0.0GlyXaa: 0.0 ± 0.0
His	3.292HisAla: 3.292 ± 0.072	0.363HisCys: 0.363 ± 0.021	1.147HisAsp: 1.147 ± 0.04	1.22HisGlu: 1.22 ± 0.032	0.96HisPhe: 0.96 ± 0.035	2.402HisGly: 2.402 ± 0.057	0.614HisHis: 0.614 ± 0.026	1.262HisIle: 1.262 ± 0.039	0.612HisLys: 0.612 ± 0.028	2.247HisLeu: 2.247 ± 0.049	0.585HisMet: 0.585 ± 0.029	0.649HisAsn: 0.649 ± 0.026	1.536HisPro: 1.536 ± 0.047	1.069HisGln: 1.069 ± 0.036	1.484HisArg: 1.484 ± 0.042	1.434HisSer: 1.434 ± 0.045	1.301HisThr: 1.301 ± 0.041	1.44HisVal: 1.44 ± 0.037	0.71HisTrp: 0.71 ± 0.029	0.801HisTyr: 0.801 ± 0.034	0.0HisXaa: 0.0 ± 0.0
Ile	6.261IleAla: 6.261 ± 0.089	0.426IleCys: 0.426 ± 0.022	2.778IleAsp: 2.778 ± 0.056	3.281IleGlu: 3.281 ± 0.066	1.165IlePhe: 1.165 ± 0.045	4.054IleGly: 4.054 ± 0.08	0.923IleHis: 0.923 ± 0.032	1.532IleIle: 1.532 ± 0.046	1.299IleLys: 1.299 ± 0.044	3.19IleLeu: 3.19 ± 0.064	0.797IleMet: 0.797 ± 0.037	1.319IleAsn: 1.319 ± 0.042	1.756IlePro: 1.756 ± 0.049	1.432IleGln: 1.432 ± 0.038	2.295IleArg: 2.295 ± 0.06	2.285IleSer: 2.285 ± 0.052	2.348IleThr: 2.348 ± 0.053	3.284IleVal: 3.284 ± 0.065	0.517IleTrp: 0.517 ± 0.027	0.982IleTyr: 0.982 ± 0.029	0.0IleXaa: 0.0 ± 0.0
Lys	3.703LysAla: 3.703 ± 0.088	0.155LysCys: 0.155 ± 0.012	1.39LysAsp: 1.39 ± 0.049	1.438LysGlu: 1.438 ± 0.051	0.641LysPhe: 0.641 ± 0.03	2.241LysGly: 2.241 ± 0.063	0.605LysHis: 0.605 ± 0.025	1.278LysIle: 1.278 ± 0.043	1.382LysLys: 1.382 ± 0.058	2.979LysLeu: 2.979 ± 0.075	0.607LysMet: 0.607 ± 0.029	0.914LysAsn: 0.914 ± 0.036	1.915LysPro: 1.915 ± 0.048	1.277LysGln: 1.277 ± 0.043	1.866LysArg: 1.866 ± 0.048	1.55LysSer: 1.55 ± 0.049	1.704LysThr: 1.704 ± 0.06	2.002LysVal: 2.002 ± 0.053	0.303LysTrp: 0.303 ± 0.02	0.567LysTyr: 0.567 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	16.481LeuAla: 16.481 ± 0.195	1.158LeuCys: 1.158 ± 0.035	5.556LeuAsp: 5.556 ± 0.095	5.084LeuGlu: 5.084 ± 0.095	3.148LeuPhe: 3.148 ± 0.071	9.508LeuGly: 9.508 ± 0.153	3.017LeuHis: 3.017 ± 0.066	4.061LeuIle: 4.061 ± 0.074	3.038LeuLys: 3.038 ± 0.074	13.772LeuLeu: 13.772 ± 0.209	2.387LeuMet: 2.387 ± 0.059	2.523LeuAsn: 2.523 ± 0.051	7.083LeuPro: 7.083 ± 0.107	7.464LeuGln: 7.464 ± 0.143	8.515LeuArg: 8.515 ± 0.101	5.637LeuSer: 5.637 ± 0.085	4.477LeuThr: 4.477 ± 0.078	6.659LeuVal: 6.659 ± 0.113	1.711LeuTrp: 1.711 ± 0.056	2.197LeuTyr: 2.197 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	3.237MetAla: 3.237 ± 0.063	0.145MetCys: 0.145 ± 0.012	1.012MetAsp: 1.012 ± 0.037	0.939MetGlu: 0.939 ± 0.033	0.577MetPhe: 0.577 ± 0.028	1.895MetGly: 1.895 ± 0.046	0.568MetHis: 0.568 ± 0.025	0.818MetIle: 0.818 ± 0.035	0.718MetLys: 0.718 ± 0.032	2.71MetLeu: 2.71 ± 0.06	0.493MetMet: 0.493 ± 0.027	0.718MetAsn: 0.718 ± 0.027	1.643MetPro: 1.643 ± 0.042	1.464MetGln: 1.464 ± 0.039	1.696MetArg: 1.696 ± 0.04	1.288MetSer: 1.288 ± 0.043	1.256MetThr: 1.256 ± 0.039	1.48MetVal: 1.48 ± 0.04	0.198MetTrp: 0.198 ± 0.014	0.356MetTyr: 0.356 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.31AsnAla: 3.31 ± 0.077	0.234AsnCys: 0.234 ± 0.019	1.133AsnAsp: 1.133 ± 0.041	1.062AsnGlu: 1.062 ± 0.039	0.794AsnPhe: 0.794 ± 0.032	2.06AsnGly: 2.06 ± 0.058	0.517AsnHis: 0.517 ± 0.024	1.136AsnIle: 1.136 ± 0.04	0.712AsnLys: 0.712 ± 0.031	2.454AsnLeu: 2.454 ± 0.05	0.525AsnMet: 0.525 ± 0.023	0.657AsnAsn: 0.657 ± 0.035	1.822AsnPro: 1.822 ± 0.051	1.034AsnGln: 1.034 ± 0.04	1.509AsnArg: 1.509 ± 0.044	1.077AsnSer: 1.077 ± 0.054	1.316AsnThr: 1.316 ± 0.043	1.608AsnVal: 1.608 ± 0.045	0.385AsnTrp: 0.385 ± 0.022	0.634AsnTyr: 0.634 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	9.114ProAla: 9.114 ± 0.158	0.455ProCys: 0.455 ± 0.023	2.801ProAsp: 2.801 ± 0.06	3.432ProGlu: 3.432 ± 0.068	1.613ProPhe: 1.613 ± 0.044	5.193ProGly: 5.193 ± 0.086	1.302ProHis: 1.302 ± 0.044	1.989ProIle: 1.989 ± 0.047	1.296ProLys: 1.296 ± 0.038	5.862ProLeu: 5.862 ± 0.104	1.4ProMet: 1.4 ± 0.036	1.248ProAsn: 1.248 ± 0.038	3.55ProPro: 3.55 ± 0.098	3.673ProGln: 3.673 ± 0.072	3.051ProArg: 3.051 ± 0.062	3.102ProSer: 3.102 ± 0.069	2.318ProThr: 2.318 ± 0.05	3.75ProVal: 3.75 ± 0.074	0.969ProTrp: 0.969 ± 0.038	1.165ProTyr: 1.165 ± 0.037	0.0ProXaa: 0.0 ± 0.0
Gln	11.181GlnAla: 11.181 ± 0.199	0.475GlnCys: 0.475 ± 0.025	2.159GlnAsp: 2.159 ± 0.05	2.463GlnGlu: 2.463 ± 0.063	1.484GlnPhe: 1.484 ± 0.043	4.858GlnGly: 4.858 ± 0.092	1.693GlnHis: 1.693 ± 0.053	2.009GlnIle: 2.009 ± 0.049	1.243GlnLys: 1.243 ± 0.038	6.374GlnLeu: 6.374 ± 0.106	1.124GlnMet: 1.124 ± 0.038	0.927GlnAsn: 0.927 ± 0.035	4.112GlnPro: 4.112 ± 0.089	4.533GlnGln: 4.533 ± 0.123	5.675GlnArg: 5.675 ± 0.102	2.6GlnSer: 2.6 ± 0.052	2.442GlnThr: 2.442 ± 0.059	3.707GlnVal: 3.707 ± 0.073	1.3GlnTrp: 1.3 ± 0.041	1.103GlnTyr: 1.103 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	7.715ArgAla: 7.715 ± 0.107	0.61ArgCys: 0.61 ± 0.029	2.985ArgAsp: 2.985 ± 0.06	3.595ArgGlu: 3.595 ± 0.076	2.478ArgPhe: 2.478 ± 0.054	4.245ArgGly: 4.245 ± 0.073	2.159ArgHis: 2.159 ± 0.054	3.726ArgIle: 3.726 ± 0.066	1.958ArgLys: 1.958 ± 0.052	8.262ArgLeu: 8.262 ± 0.106	1.845ArgMet: 1.845 ± 0.043	1.717ArgAsn: 1.717 ± 0.046	3.515ArgPro: 3.515 ± 0.067	4.671ArgGln: 4.671 ± 0.087	4.873ArgArg: 4.873 ± 0.087	3.592ArgSer: 3.592 ± 0.072	2.736ArgThr: 2.736 ± 0.047	4.108ArgVal: 4.108 ± 0.082	1.325ArgTrp: 1.325 ± 0.044	1.911ArgTyr: 1.911 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	7.254SerAla: 7.254 ± 0.102	0.397SerCys: 0.397 ± 0.025	2.386SerAsp: 2.386 ± 0.053	2.22SerGlu: 2.22 ± 0.054	1.738SerPhe: 1.738 ± 0.048	5.159SerGly: 5.159 ± 0.09	1.283SerHis: 1.283 ± 0.042	2.234SerIle: 2.234 ± 0.051	1.414SerLys: 1.414 ± 0.047	5.39SerLeu: 5.39 ± 0.079	1.15SerMet: 1.15 ± 0.033	1.273SerAsn: 1.273 ± 0.047	2.947SerPro: 2.947 ± 0.067	2.366SerGln: 2.366 ± 0.058	2.933SerArg: 2.933 ± 0.062	3.001SerSer: 3.001 ± 0.071	2.427SerThr: 2.427 ± 0.064	3.29SerVal: 3.29 ± 0.065	0.771SerTrp: 0.771 ± 0.031	1.263SerTyr: 1.263 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.84ThrAla: 5.84 ± 0.093	0.35ThrCys: 0.35 ± 0.026	1.955ThrAsp: 1.955 ± 0.048	1.999ThrGlu: 1.999 ± 0.05	1.329ThrPhe: 1.329 ± 0.042	3.896ThrGly: 3.896 ± 0.066	1.068ThrHis: 1.068 ± 0.038	2.038ThrIle: 2.038 ± 0.05	1.012ThrLys: 1.012 ± 0.033	5.711ThrLeu: 5.711 ± 0.083	0.902ThrMet: 0.902 ± 0.032	1.007ThrAsn: 1.007 ± 0.042	3.542ThrPro: 3.542 ± 0.076	2.172ThrGln: 2.172 ± 0.048	2.816ThrArg: 2.816 ± 0.055	2.185ThrSer: 2.185 ± 0.056	2.357ThrThr: 2.357 ± 0.064	3.351ThrVal: 3.351 ± 0.078	0.562ThrTrp: 0.562 ± 0.026	0.871ThrTyr: 0.871 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	7.908ValAla: 7.908 ± 0.118	0.634ValCys: 0.634 ± 0.028	3.158ValAsp: 3.158 ± 0.063	3.056ValGlu: 3.056 ± 0.066	2.41ValPhe: 2.41 ± 0.057	4.552ValGly: 4.552 ± 0.082	1.562ValHis: 1.562 ± 0.043	2.844ValIle: 2.844 ± 0.059	1.831ValLys: 1.831 ± 0.058	8.099ValLeu: 8.099 ± 0.129	1.593ValMet: 1.593 ± 0.047	1.711ValAsn: 1.711 ± 0.05	3.546ValPro: 3.546 ± 0.072	4.152ValGln: 4.152 ± 0.075	4.577ValArg: 4.577 ± 0.066	3.05ValSer: 3.05 ± 0.064	3.178ValThr: 3.178 ± 0.075	4.874ValVal: 4.874 ± 0.094	0.898ValTrp: 0.898 ± 0.03	1.412ValTyr: 1.412 ± 0.04	0.0ValXaa: 0.0 ± 0.0
Trp	1.737TrpAla: 1.737 ± 0.054	0.201TrpCys: 0.201 ± 0.016	0.644TrpAsp: 0.644 ± 0.026	0.515TrpGlu: 0.515 ± 0.025	0.545TrpPhe: 0.545 ± 0.029	1.102TrpGly: 1.102 ± 0.032	0.516TrpHis: 0.516 ± 0.027	0.606TrpIle: 0.606 ± 0.025	0.317TrpLys: 0.317 ± 0.019	2.783TrpLeu: 2.783 ± 0.072	0.399TrpMet: 0.399 ± 0.023	0.344TrpAsn: 0.344 ± 0.021	0.997TrpPro: 0.997 ± 0.039	1.429TrpGln: 1.429 ± 0.048	1.488TrpArg: 1.488 ± 0.045	0.721TrpSer: 0.721 ± 0.027	0.474TrpThr: 0.474 ± 0.025	0.931TrpVal: 0.931 ± 0.031	0.31TrpTrp: 0.31 ± 0.018	0.323TrpTyr: 0.323 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.672TyrAla: 2.672 ± 0.063	0.239TyrCys: 0.239 ± 0.016	1.235TyrAsp: 1.235 ± 0.043	1.234TyrGlu: 1.234 ± 0.042	0.844TyrPhe: 0.844 ± 0.032	1.994TyrGly: 1.994 ± 0.054	0.519TyrHis: 0.519 ± 0.025	0.819TyrIle: 0.819 ± 0.029	0.59TyrLys: 0.59 ± 0.032	2.421TyrLeu: 2.421 ± 0.057	0.393TyrMet: 0.393 ± 0.021	0.557TyrAsn: 0.557 ± 0.029	1.092TyrPro: 1.092 ± 0.038	1.148TyrGln: 1.148 ± 0.041	1.695TyrArg: 1.695 ± 0.041	1.138TyrSer: 1.138 ± 0.038	1.168TyrThr: 1.168 ± 0.038	1.495TyrVal: 1.495 ± 0.038	0.39TyrTrp: 0.39 ± 0.02	0.594TyrTyr: 0.594 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2590 proteins (887569 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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