Rhodobacteraceae bacterium THAF1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P5X3L8|A0A3P5X3L8_9RHOB Uncharacterized protein OS=Rhodobacteraceae bacterium THAF1 OX=2483814 GN=PARPLA_02764 PE=4 SV=1
MM1 pKa = 7.23MSKK4 pKa = 10.46NLLITTALCVAGLPALAQDD23 pKa = 4.04TEE25 pKa = 4.9CPVKK29 pKa = 10.93VGVLHH34 pKa = 6.32SLSGTMAISEE44 pKa = 4.52TTLKK48 pKa = 10.88DD49 pKa = 3.37AMLMLVEE56 pKa = 4.65KK57 pKa = 10.77QNADD61 pKa = 3.33GGLLGCQIEE70 pKa = 4.68TVVVDD75 pKa = 4.89PASDD79 pKa = 3.25WPRR82 pKa = 11.84FAEE85 pKa = 5.21LGRR88 pKa = 11.84QLLTEE93 pKa = 4.75DD94 pKa = 3.78EE95 pKa = 4.31VDD97 pKa = 3.86VIFGSWTSVSRR108 pKa = 11.84KK109 pKa = 9.33SVLPVLEE116 pKa = 4.27EE117 pKa = 4.15LNGLMFYY124 pKa = 10.18PVQYY128 pKa = 10.42EE129 pKa = 4.3GEE131 pKa = 4.12EE132 pKa = 3.94SSKK135 pKa = 11.08NVFYY139 pKa = 10.71TGAAPNQQAIPAVDD153 pKa = 3.57YY154 pKa = 10.58FLEE157 pKa = 4.18EE158 pKa = 4.11LGVEE162 pKa = 4.36KK163 pKa = 10.21FALLGTDD170 pKa = 3.23YY171 pKa = 11.13VYY173 pKa = 11.01PRR175 pKa = 11.84TTNNILEE182 pKa = 4.75SYY184 pKa = 10.04LQEE187 pKa = 3.84QGIAEE192 pKa = 3.95EE193 pKa = 5.25DD194 pKa = 3.28IFVNYY199 pKa = 9.64TPFGHH204 pKa = 6.47SDD206 pKa = 2.58WSTIVSDD213 pKa = 4.27VIEE216 pKa = 4.75LGSDD220 pKa = 4.15GSQVGVISTINGDD233 pKa = 3.92ANVGFYY239 pKa = 10.75KK240 pKa = 10.48EE241 pKa = 4.68LAAQDD246 pKa = 3.83VSADD250 pKa = 4.36DD251 pKa = 3.79IPVVAFSVGEE261 pKa = 4.07EE262 pKa = 4.18EE263 pKa = 5.95LSGLDD268 pKa = 3.24TSNLVGHH275 pKa = 6.85LAAWNYY281 pKa = 7.58FQSAEE286 pKa = 4.14SPEE289 pKa = 4.02NEE291 pKa = 4.11EE292 pKa = 6.12FIASWKK298 pKa = 10.3EE299 pKa = 3.73FIGDD303 pKa = 3.73EE304 pKa = 4.12NRR306 pKa = 11.84VTNDD310 pKa = 2.86PMEE313 pKa = 3.81AHH315 pKa = 6.65YY316 pKa = 10.6IGFNMWVNAVEE327 pKa = 4.26EE328 pKa = 4.46AGTTDD333 pKa = 2.78VDD335 pKa = 3.98AVRR338 pKa = 11.84EE339 pKa = 4.19AMWGQEE345 pKa = 3.93YY346 pKa = 10.11PNLTGGTAVMGTNHH360 pKa = 6.9HH361 pKa = 6.3LAKK364 pKa = 9.98PVLIGEE370 pKa = 4.31ITEE373 pKa = 5.14DD374 pKa = 3.67GQFDD378 pKa = 4.59IISEE382 pKa = 4.18TDD384 pKa = 3.79PVPGDD389 pKa = 2.9AWTDD393 pKa = 3.87FLPDD397 pKa = 3.59SAVLEE402 pKa = 4.54SDD404 pKa = 3.48WSEE407 pKa = 4.33LDD409 pKa = 3.02CGMYY413 pKa = 8.93NTEE416 pKa = 4.47TEE418 pKa = 4.25SCVQQTSNYY427 pKa = 9.55

Molecular weight: 46.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P5X748|A0A3P5X748_9RHOB SoxAX cytochrome complex subunit A OS=Rhodobacteraceae bacterium THAF1 OX=2483814 GN=soxA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39AGRR28 pKa = 11.84TILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.25KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3313

0

3313

1009387

30

5844

304.7

32.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.498 ± 0.056

0.869 ± 0.013

6.708 ± 0.049

5.65 ± 0.045

3.603 ± 0.026

8.841 ± 0.049

1.989 ± 0.023

5.141 ± 0.031

2.775 ± 0.038

9.826 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.773 ± 0.027

2.452 ± 0.027

5.127 ± 0.033

3.145 ± 0.026

6.833 ± 0.059

5.013 ± 0.028

5.822 ± 0.056

7.392 ± 0.038

1.409 ± 0.019

2.133 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski