Marinicauda algicola
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3306 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S2GZ53|A0A4S2GZ53_9PROT YjbQ family protein OS=Marinicauda algicola OX=2029849 GN=E5163_11115 PE=3 SV=1
MM1 pKa = 7.19 KK2 pKa = 9.76 TLLLASAAALVAVSGASAQEE22 pKa = 3.58 AGRR25 pKa = 11.84 FYY27 pKa = 11.28 AGAGYY32 pKa = 8.63 TYY34 pKa = 11.21 VDD36 pKa = 3.97 FDD38 pKa = 3.9 DD39 pKa = 5.79 VEE41 pKa = 4.9 FNTLNLRR48 pKa = 11.84 GGYY51 pKa = 10.02 DD52 pKa = 3.42 FNEE55 pKa = 4.46 FVALEE60 pKa = 4.19 GEE62 pKa = 4.59 VLVGLGDD69 pKa = 3.72 EE70 pKa = 4.98 DD71 pKa = 4.08 VDD73 pKa = 5.51 LGGTTADD80 pKa = 3.23 VSLDD84 pKa = 3.56 YY85 pKa = 11.52 ALGAFVKK92 pKa = 10.19 GQYY95 pKa = 9.06 PVSEE99 pKa = 4.19 QVSLFARR106 pKa = 11.84 AGYY109 pKa = 10.8 AYY111 pKa = 9.81 MEE113 pKa = 4.75 AEE115 pKa = 4.07 ASASGVTLSDD125 pKa = 3.51 DD126 pKa = 3.66 TDD128 pKa = 3.58 GFAYY132 pKa = 10.21 GAGAEE137 pKa = 4.2 WAFAGPNAVRR147 pKa = 11.84 VEE149 pKa = 3.88 YY150 pKa = 9.91 TRR152 pKa = 11.84 YY153 pKa = 10.06 EE154 pKa = 4.15 FEE156 pKa = 5.82 DD157 pKa = 3.95 DD158 pKa = 4.41 AEE160 pKa = 5.2 ADD162 pKa = 3.97 SFGATYY168 pKa = 10.23 IRR170 pKa = 11.84 RR171 pKa = 11.84 FF172 pKa = 3.42
Molecular weight: 18.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.783
IPC_protein 3.757
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.465
Solomon 3.732
Lehninger 3.694
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.884
Patrickios 0.693
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.777
Protein with the highest isoelectric point:
>tr|A0A4S2H3D5|A0A4S2H3D5_9PROT DUF2793 domain-containing protein OS=Marinicauda algicola OX=2029849 GN=E5163_01065 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.59 RR3 pKa = 11.84 TFQPSKK9 pKa = 9.84 LVRR12 pKa = 11.84 KK13 pKa = 9.15 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.42 AGRR28 pKa = 11.84 QVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.98 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3306
0
3306
1029613
25
1664
311.4
33.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.781 ± 0.074
0.767 ± 0.013
5.893 ± 0.031
7.056 ± 0.044
3.735 ± 0.028
8.919 ± 0.039
1.943 ± 0.022
4.605 ± 0.033
2.63 ± 0.044
9.969 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.186 ± 0.021
2.119 ± 0.025
5.248 ± 0.032
2.791 ± 0.022
7.904 ± 0.042
4.929 ± 0.031
4.877 ± 0.033
7.092 ± 0.036
1.379 ± 0.018
2.179 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here