Microbacterium sp. SLBN-1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A560MNA6|A0A560MNA6_9MICO MFS transporter OS=Microbacterium sp. SLBN-1 OX=2768438 GN=FBY16_1282 PE=4 SV=1
MM1 pKa = 7.59ARR3 pKa = 11.84NTHH6 pKa = 4.75HH7 pKa = 6.54TARR10 pKa = 11.84FGFPGPALRR19 pKa = 11.84RR20 pKa = 11.84AAAGLAVVTVGALALSACTTSTEE43 pKa = 4.67DD44 pKa = 3.37PGTTEE49 pKa = 4.84GGTLKK54 pKa = 10.42LWHH57 pKa = 6.48YY58 pKa = 9.86EE59 pKa = 4.19GADD62 pKa = 3.47SAMGKK67 pKa = 9.56AWAEE71 pKa = 4.07AISVFEE77 pKa = 4.52EE78 pKa = 4.02EE79 pKa = 3.8TGATVEE85 pKa = 4.18FEE87 pKa = 4.46EE88 pKa = 5.81KK89 pKa = 10.51SFEE92 pKa = 4.27QIQSTASQVLDD103 pKa = 3.35TDD105 pKa = 4.22AAPDD109 pKa = 3.44LMEE112 pKa = 4.6FNKK115 pKa = 10.57GNATAGFLASTGLISDD131 pKa = 3.71ISDD134 pKa = 3.35AVDD137 pKa = 3.37EE138 pKa = 4.73YY139 pKa = 11.84GWADD143 pKa = 3.46KK144 pKa = 10.46LAPSLQTTAKK154 pKa = 8.49YY155 pKa = 9.42TDD157 pKa = 4.02DD158 pKa = 3.59GVMGGDD164 pKa = 3.07TWYY167 pKa = 10.81GIPNYY172 pKa = 10.79GEE174 pKa = 4.11FVGVYY179 pKa = 9.96YY180 pKa = 10.98NLDD183 pKa = 3.38AFADD187 pKa = 3.73AGLEE191 pKa = 4.16VPTTYY196 pKa = 11.19DD197 pKa = 2.87EE198 pKa = 4.8FVAVLDD204 pKa = 3.85AFVAKK209 pKa = 10.61GITPLAEE216 pKa = 4.56AGAEE220 pKa = 4.13YY221 pKa = 10.11PLGQLWYY228 pKa = 10.59QLALSQGDD236 pKa = 3.54RR237 pKa = 11.84QFVNDD242 pKa = 3.53YY243 pKa = 10.58QLYY246 pKa = 8.78EE247 pKa = 4.77NPVDD251 pKa = 3.49WQGPEE256 pKa = 3.33ITYY259 pKa = 10.69ASQTLADD266 pKa = 4.15YY267 pKa = 11.04VEE269 pKa = 4.43KK270 pKa = 10.81GYY272 pKa = 10.3IASDD276 pKa = 3.62VSSVKK281 pKa = 10.95AEE283 pKa = 3.91DD284 pKa = 3.76AGVSFINGTAPIFVSGSWWYY304 pKa = 10.97GRR306 pKa = 11.84FVSEE310 pKa = 4.2ATGFDD315 pKa = 3.22WTMTAFPEE323 pKa = 4.33ATLSLGSSGNLWVVPEE339 pKa = 3.88NAANKK344 pKa = 8.56EE345 pKa = 3.81LAYY348 pKa = 10.18EE349 pKa = 4.84FIDD352 pKa = 2.86ITMRR356 pKa = 11.84PEE358 pKa = 3.16IQAIIGNNGGVPVAADD374 pKa = 3.41PADD377 pKa = 3.45ITDD380 pKa = 3.84EE381 pKa = 4.25KK382 pKa = 10.65SQEE385 pKa = 4.36LITVFNGILEE395 pKa = 4.4ADD397 pKa = 3.93GLSFYY402 pKa = 10.21PDD404 pKa = 2.74WPAPGFYY411 pKa = 10.52DD412 pKa = 4.99VIVQQLQGLVTGSQDD427 pKa = 3.5VATTNTNLGAQYY439 pKa = 11.15DD440 pKa = 4.03EE441 pKa = 4.46GTADD445 pKa = 3.63FRR447 pKa = 5.5

Molecular weight: 47.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A560MKL4|A0A560MKL4_9MICO DUF1508 domain-containing protein OS=Microbacterium sp. SLBN-1 OX=2768438 GN=FBY16_0316 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2917

0

2917

977528

29

3537

335.1

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.898 ± 0.068

0.457 ± 0.011

6.535 ± 0.042

5.462 ± 0.039

3.116 ± 0.029

8.832 ± 0.043

1.978 ± 0.024

4.321 ± 0.033

1.796 ± 0.029

10.057 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.677 ± 0.02

1.792 ± 0.027

5.403 ± 0.031

2.622 ± 0.02

7.487 ± 0.057

5.573 ± 0.035

6.259 ± 0.054

9.201 ± 0.052

1.565 ± 0.025

1.971 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski