Amino acid dipepetide frequency for Microbacterium sp. SLBN-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.565AlaAla: 21.565 ± 0.237
0.637AlaCys: 0.637 ± 0.022
9.239AlaAsp: 9.239 ± 0.112
8.071AlaGlu: 8.071 ± 0.124
4.007AlaPhe: 4.007 ± 0.072
12.114AlaGly: 12.114 ± 0.111
2.649AlaHis: 2.649 ± 0.057
5.586AlaIle: 5.586 ± 0.09
2.471AlaLys: 2.471 ± 0.068
14.589AlaLeu: 14.589 ± 0.159
2.534AlaMet: 2.534 ± 0.055
2.087AlaAsn: 2.087 ± 0.043
6.925AlaPro: 6.925 ± 0.118
3.732AlaGln: 3.732 ± 0.063
9.968AlaArg: 9.968 ± 0.153
7.659AlaSer: 7.659 ± 0.096
8.0AlaThr: 8.0 ± 0.113
12.548AlaVal: 12.548 ± 0.13
2.084AlaTrp: 2.084 ± 0.054
2.521AlaTyr: 2.521 ± 0.053
0.0AlaXaa: 0.0 ± 0.0
Cys
0.659CysAla: 0.659 ± 0.027
0.046CysCys: 0.046 ± 0.007
0.324CysAsp: 0.324 ± 0.017
0.244CysGlu: 0.244 ± 0.018
0.144CysPhe: 0.144 ± 0.012
0.516CysGly: 0.516 ± 0.025
0.112CysHis: 0.112 ± 0.011
0.17CysIle: 0.17 ± 0.013
0.056CysLys: 0.056 ± 0.008
0.385CysLeu: 0.385 ± 0.018
0.066CysMet: 0.066 ± 0.008
0.082CysAsn: 0.082 ± 0.01
0.256CysPro: 0.256 ± 0.018
0.102CysGln: 0.102 ± 0.011
0.327CysArg: 0.327 ± 0.021
0.262CysSer: 0.262 ± 0.016
0.297CysThr: 0.297 ± 0.023
0.379CysVal: 0.379 ± 0.02
0.058CysTrp: 0.058 ± 0.008
0.088CysTyr: 0.088 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
10.468AspAla: 10.468 ± 0.118
0.237AspCys: 0.237 ± 0.017
4.786AspAsp: 4.786 ± 0.085
4.212AspGlu: 4.212 ± 0.073
1.786AspPhe: 1.786 ± 0.042
6.683AspGly: 6.683 ± 0.1
1.228AspHis: 1.228 ± 0.039
2.441AspIle: 2.441 ± 0.05
0.892AspLys: 0.892 ± 0.033
6.601AspLeu: 6.601 ± 0.086
0.841AspMet: 0.841 ± 0.028
0.956AspAsn: 0.956 ± 0.031
4.216AspPro: 4.216 ± 0.075
1.414AspGln: 1.414 ± 0.041
4.781AspArg: 4.781 ± 0.084
2.543AspSer: 2.543 ± 0.058
3.039AspThr: 3.039 ± 0.058
6.038AspVal: 6.038 ± 0.087
0.916AspTrp: 0.916 ± 0.034
1.27AspTyr: 1.27 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
6.988GluAla: 6.988 ± 0.105
0.218GluCys: 0.218 ± 0.015
2.639GluAsp: 2.639 ± 0.06
2.992GluGlu: 2.992 ± 0.064
1.682GluPhe: 1.682 ± 0.043
4.126GluGly: 4.126 ± 0.073
1.273GluHis: 1.273 ± 0.037
2.647GluIle: 2.647 ± 0.059
1.337GluLys: 1.337 ± 0.041
6.193GluLeu: 6.193 ± 0.09
0.849GluMet: 0.849 ± 0.029
1.184GluAsn: 1.184 ± 0.037
2.872GluPro: 2.872 ± 0.06
1.912GluGln: 1.912 ± 0.048
5.118GluArg: 5.118 ± 0.085
2.854GluSer: 2.854 ± 0.057
2.989GluThr: 2.989 ± 0.05
4.707GluVal: 4.707 ± 0.075
0.884GluTrp: 0.884 ± 0.031
1.154GluTyr: 1.154 ± 0.036
0.0GluXaa: 0.0 ± 0.0
Phe
4.46PheAla: 4.46 ± 0.072
0.135PheCys: 0.135 ± 0.012
2.405PheAsp: 2.405 ± 0.054
1.69PheGlu: 1.69 ± 0.047
1.088PhePhe: 1.088 ± 0.037
3.278PheGly: 3.278 ± 0.067
0.541PheHis: 0.541 ± 0.025
1.1PheIle: 1.1 ± 0.035
0.395PheLys: 0.395 ± 0.02
2.974PheLeu: 2.974 ± 0.065
0.408PheMet: 0.408 ± 0.021
0.61PheAsn: 0.61 ± 0.024
1.406PhePro: 1.406 ± 0.04
0.781PheGln: 0.781 ± 0.03
1.921PheArg: 1.921 ± 0.048
1.762PheSer: 1.762 ± 0.043
2.121PheThr: 2.121 ± 0.047
2.917PheVal: 2.917 ± 0.066
0.529PheTrp: 0.529 ± 0.026
0.64PheTyr: 0.64 ± 0.029
0.0PheXaa: 0.0 ± 0.0
Gly
10.917GlyAla: 10.917 ± 0.114
0.502GlyCys: 0.502 ± 0.022
5.485GlyAsp: 5.485 ± 0.084
4.884GlyGlu: 4.884 ± 0.082
3.233GlyPhe: 3.233 ± 0.057
7.618GlyGly: 7.618 ± 0.124
1.803GlyHis: 1.803 ± 0.043
4.854GlyIle: 4.854 ± 0.088
2.031GlyLys: 2.031 ± 0.052
8.443GlyLeu: 8.443 ± 0.106
1.893GlyMet: 1.893 ± 0.045
1.593GlyAsn: 1.593 ± 0.048
3.682GlyPro: 3.682 ± 0.059
2.362GlyGln: 2.362 ± 0.047
6.342GlyArg: 6.342 ± 0.094
5.212GlySer: 5.212 ± 0.079
5.663GlyThr: 5.663 ± 0.101
7.932GlyVal: 7.932 ± 0.082
1.66GlyTrp: 1.66 ± 0.046
2.208GlyTyr: 2.208 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
2.54HisAla: 2.54 ± 0.059
0.108HisCys: 0.108 ± 0.01
1.319HisAsp: 1.319 ± 0.039
1.045HisGlu: 1.045 ± 0.036
0.537HisPhe: 0.537 ± 0.025
1.973HisGly: 1.973 ± 0.049
0.55HisHis: 0.55 ± 0.026
0.74HisIle: 0.74 ± 0.027
0.252HisLys: 0.252 ± 0.015
2.034HisLeu: 2.034 ± 0.052
0.305HisMet: 0.305 ± 0.019
0.358HisAsn: 0.358 ± 0.019
1.533HisPro: 1.533 ± 0.045
0.465HisGln: 0.465 ± 0.022
1.636HisArg: 1.636 ± 0.047
0.927HisSer: 0.927 ± 0.032
1.018HisThr: 1.018 ± 0.034
1.765HisVal: 1.765 ± 0.045
0.295HisTrp: 0.295 ± 0.021
0.385HisTyr: 0.385 ± 0.017
0.0HisXaa: 0.0 ± 0.0
Ile
6.839IleAla: 6.839 ± 0.092
0.183IleCys: 0.183 ± 0.015
3.569IleAsp: 3.569 ± 0.064
2.662IleGlu: 2.662 ± 0.064
1.1IlePhe: 1.1 ± 0.036
4.256IleGly: 4.256 ± 0.076
0.714IleHis: 0.714 ± 0.029
1.575IleIle: 1.575 ± 0.047
0.685IleLys: 0.685 ± 0.031
3.675IleLeu: 3.675 ± 0.066
0.539IleMet: 0.539 ± 0.021
0.773IleAsn: 0.773 ± 0.03
2.382IlePro: 2.382 ± 0.049
0.913IleGln: 0.913 ± 0.029
2.591IleArg: 2.591 ± 0.051
2.228IleSer: 2.228 ± 0.045
2.562IleThr: 2.562 ± 0.065
4.821IleVal: 4.821 ± 0.075
0.475IleTrp: 0.475 ± 0.024
0.671IleTyr: 0.671 ± 0.028
0.0IleXaa: 0.0 ± 0.0
Lys
2.214LysAla: 2.214 ± 0.063
0.054LysCys: 0.054 ± 0.007
1.011LysAsp: 1.011 ± 0.036
0.865LysGlu: 0.865 ± 0.034
0.439LysPhe: 0.439 ± 0.021
1.435LysGly: 1.435 ± 0.048
0.366LysHis: 0.366 ± 0.021
0.834LysIle: 0.834 ± 0.032
0.755LysLys: 0.755 ± 0.038
1.694LysLeu: 1.694 ± 0.042
0.33LysMet: 0.33 ± 0.017
0.441LysAsn: 0.441 ± 0.022
1.013LysPro: 1.013 ± 0.034
0.552LysGln: 0.552 ± 0.024
1.336LysArg: 1.336 ± 0.046
1.057LysSer: 1.057 ± 0.042
1.297LysThr: 1.297 ± 0.045
1.631LysVal: 1.631 ± 0.048
0.228LysTrp: 0.228 ± 0.015
0.409LysTyr: 0.409 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
14.788LeuAla: 14.788 ± 0.146
0.46LeuCys: 0.46 ± 0.023
6.858LeuAsp: 6.858 ± 0.094
4.815LeuGlu: 4.815 ± 0.076
2.958LeuPhe: 2.958 ± 0.066
8.662LeuGly: 8.662 ± 0.098
1.894LeuHis: 1.894 ± 0.052
4.033LeuIle: 4.033 ± 0.078
1.562LeuLys: 1.562 ± 0.049
10.343LeuLeu: 10.343 ± 0.132
1.513LeuMet: 1.513 ± 0.048
1.655LeuAsn: 1.655 ± 0.037
5.625LeuPro: 5.625 ± 0.083
2.534LeuGln: 2.534 ± 0.052
7.873LeuArg: 7.873 ± 0.096
5.913LeuSer: 5.913 ± 0.075
6.642LeuThr: 6.642 ± 0.089
9.402LeuVal: 9.402 ± 0.121
1.372LeuTrp: 1.372 ± 0.041
1.667LeuTyr: 1.667 ± 0.045
0.0LeuXaa: 0.0 ± 0.0
Met
1.943MetAla: 1.943 ± 0.05
0.088MetCys: 0.088 ± 0.01
0.736MetAsp: 0.736 ± 0.027
0.563MetGlu: 0.563 ± 0.027
0.487MetPhe: 0.487 ± 0.026
1.225MetGly: 1.225 ± 0.041
0.298MetHis: 0.298 ± 0.015
0.832MetIle: 0.832 ± 0.03
0.379MetLys: 0.379 ± 0.022
1.731MetLeu: 1.731 ± 0.042
0.299MetMet: 0.299 ± 0.017
0.438MetAsn: 0.438 ± 0.021
1.128MetPro: 1.128 ± 0.036
0.474MetGln: 0.474 ± 0.022
1.31MetArg: 1.31 ± 0.036
1.467MetSer: 1.467 ± 0.038
1.686MetThr: 1.686 ± 0.042
1.238MetVal: 1.238 ± 0.037
0.204MetTrp: 0.204 ± 0.015
0.246MetTyr: 0.246 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.242AsnAla: 2.242 ± 0.051
0.085AsnCys: 0.085 ± 0.009
1.164AsnAsp: 1.164 ± 0.04
0.903AsnGlu: 0.903 ± 0.032
0.578AsnPhe: 0.578 ± 0.024
1.723AsnGly: 1.723 ± 0.056
0.341AsnHis: 0.341 ± 0.019
0.711AsnIle: 0.711 ± 0.03
0.344AsnLys: 0.344 ± 0.021
1.765AsnLeu: 1.765 ± 0.04
0.272AsnMet: 0.272 ± 0.015
0.445AsnAsn: 0.445 ± 0.028
1.438AsnPro: 1.438 ± 0.044
0.481AsnGln: 0.481 ± 0.025
1.164AsnArg: 1.164 ± 0.034
0.88AsnSer: 0.88 ± 0.035
1.116AsnThr: 1.116 ± 0.044
1.563AsnVal: 1.563 ± 0.039
0.272AsnTrp: 0.272 ± 0.016
0.432AsnTyr: 0.432 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
7.563ProAla: 7.563 ± 0.113
0.168ProCys: 0.168 ± 0.015
4.167ProAsp: 4.167 ± 0.061
3.589ProGlu: 3.589 ± 0.063
1.716ProPhe: 1.716 ± 0.038
4.822ProGly: 4.822 ± 0.063
1.189ProHis: 1.189 ± 0.035
1.974ProIle: 1.974 ± 0.041
0.857ProLys: 0.857 ± 0.033
5.145ProLeu: 5.145 ± 0.08
0.813ProMet: 0.813 ± 0.029
0.833ProAsn: 0.833 ± 0.026
2.348ProPro: 2.348 ± 0.063
1.456ProGln: 1.456 ± 0.034
3.635ProArg: 3.635 ± 0.065
3.15ProSer: 3.15 ± 0.061
3.713ProThr: 3.713 ± 0.079
4.943ProVal: 4.943 ± 0.086
0.936ProTrp: 0.936 ± 0.032
1.014ProTyr: 1.014 ± 0.043
0.0ProXaa: 0.0 ± 0.0
Gln
3.339GlnAla: 3.339 ± 0.057
0.092GlnCys: 0.092 ± 0.009
1.229GlnAsp: 1.229 ± 0.035
1.29GlnGlu: 1.29 ± 0.039
0.833GlnPhe: 0.833 ± 0.028
1.913GlnGly: 1.913 ± 0.046
0.577GlnHis: 0.577 ± 0.023
1.302GlnIle: 1.302 ± 0.04
0.593GlnLys: 0.593 ± 0.025
2.928GlnLeu: 2.928 ± 0.054
0.44GlnMet: 0.44 ± 0.02
0.592GlnAsn: 0.592 ± 0.029
1.353GlnPro: 1.353 ± 0.041
1.021GlnGln: 1.021 ± 0.034
2.411GlnArg: 2.411 ± 0.056
1.388GlnSer: 1.388 ± 0.036
1.604GlnThr: 1.604 ± 0.042
2.21GlnVal: 2.21 ± 0.045
0.502GlnTrp: 0.502 ± 0.024
0.598GlnTyr: 0.598 ± 0.026
0.0GlnXaa: 0.0 ± 0.0
Arg
9.534ArgAla: 9.534 ± 0.129
0.32ArgCys: 0.32 ± 0.022
4.692ArgAsp: 4.692 ± 0.083
4.458ArgGlu: 4.458 ± 0.079
2.403ArgPhe: 2.403 ± 0.051
5.846ArgGly: 5.846 ± 0.094
1.714ArgHis: 1.714 ± 0.051
3.579ArgIle: 3.579 ± 0.063
1.203ArgLys: 1.203 ± 0.04
7.496ArgLeu: 7.496 ± 0.105
1.605ArgMet: 1.605 ± 0.039
1.236ArgAsn: 1.236 ± 0.035
3.822ArgPro: 3.822 ± 0.073
1.957ArgGln: 1.957 ± 0.043
7.04ArgArg: 7.04 ± 0.108
4.014ArgSer: 4.014 ± 0.063
4.538ArgThr: 4.538 ± 0.074
6.546ArgVal: 6.546 ± 0.093
1.269ArgTrp: 1.269 ± 0.041
1.594ArgTyr: 1.594 ± 0.042
0.0ArgXaa: 0.0 ± 0.0
Ser
7.443SerAla: 7.443 ± 0.108
0.239SerCys: 0.239 ± 0.017
3.347SerAsp: 3.347 ± 0.066
2.767SerGlu: 2.767 ± 0.055
1.925SerPhe: 1.925 ± 0.048
5.782SerGly: 5.782 ± 0.083
1.02SerHis: 1.02 ± 0.034
2.388SerIle: 2.388 ± 0.051
0.935SerLys: 0.935 ± 0.033
5.361SerLeu: 5.361 ± 0.076
1.13SerMet: 1.13 ± 0.032
0.954SerAsn: 0.954 ± 0.034
3.247SerPro: 3.247 ± 0.059
1.321SerGln: 1.321 ± 0.033
3.875SerArg: 3.875 ± 0.064
3.433SerSer: 3.433 ± 0.07
3.633SerThr: 3.633 ± 0.073
4.822SerVal: 4.822 ± 0.081
0.915SerTrp: 0.915 ± 0.032
1.189SerTyr: 1.189 ± 0.036
0.0SerXaa: 0.0 ± 0.0
Thr
8.464ThrAla: 8.464 ± 0.103
0.279ThrCys: 0.279 ± 0.02
3.862ThrAsp: 3.862 ± 0.058
2.903ThrGlu: 2.903 ± 0.055
1.997ThrPhe: 1.997 ± 0.048
5.847ThrGly: 5.847 ± 0.088
1.184ThrHis: 1.184 ± 0.033
2.829ThrIle: 2.829 ± 0.071
1.107ThrLys: 1.107 ± 0.034
6.1ThrLeu: 6.1 ± 0.088
0.927ThrMet: 0.927 ± 0.032
1.139ThrAsn: 1.139 ± 0.043
4.243ThrPro: 4.243 ± 0.076
1.484ThrGln: 1.484 ± 0.042
3.922ThrArg: 3.922 ± 0.063
3.653ThrSer: 3.653 ± 0.071
4.09ThrThr: 4.09 ± 0.084
6.324ThrVal: 6.324 ± 0.113
1.014ThrTrp: 1.014 ± 0.039
1.217ThrTyr: 1.217 ± 0.039
0.0ThrXaa: 0.0 ± 0.0
Val
12.741ValAla: 12.741 ± 0.128
0.509ValCys: 0.509 ± 0.026
6.269ValAsp: 6.269 ± 0.074
4.816ValGlu: 4.816 ± 0.065
2.972ValPhe: 2.972 ± 0.058
7.441ValGly: 7.441 ± 0.104
1.694ValHis: 1.694 ± 0.048
4.209ValIle: 4.209 ± 0.065
1.503ValLys: 1.503 ± 0.041
9.285ValLeu: 9.285 ± 0.108
1.38ValMet: 1.38 ± 0.045
1.67ValAsn: 1.67 ± 0.043
4.796ValPro: 4.796 ± 0.073
2.156ValGln: 2.156 ± 0.049
6.628ValArg: 6.628 ± 0.094
5.306ValSer: 5.306 ± 0.078
6.301ValThr: 6.301 ± 0.124
9.383ValVal: 9.383 ± 0.113
1.329ValTrp: 1.329 ± 0.039
1.62ValTyr: 1.62 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
1.678TrpAla: 1.678 ± 0.044
0.097TrpCys: 0.097 ± 0.011
0.887TrpAsp: 0.887 ± 0.031
0.686TrpGlu: 0.686 ± 0.031
0.576TrpPhe: 0.576 ± 0.028
1.186TrpGly: 1.186 ± 0.04
0.36TrpHis: 0.36 ± 0.018
0.73TrpIle: 0.73 ± 0.028
0.262TrpLys: 0.262 ± 0.019
1.756TrpLeu: 1.756 ± 0.053
0.362TrpMet: 0.362 ± 0.019
0.437TrpAsn: 0.437 ± 0.022
0.736TrpPro: 0.736 ± 0.029
0.55TrpGln: 0.55 ± 0.025
1.353TrpArg: 1.353 ± 0.045
1.014TrpSer: 1.014 ± 0.033
1.034TrpThr: 1.034 ± 0.034
1.231TrpVal: 1.231 ± 0.035
0.387TrpTrp: 0.387 ± 0.021
0.326TrpTyr: 0.326 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.598TyrAla: 2.598 ± 0.06
0.113TyrCys: 0.113 ± 0.011
1.361TyrAsp: 1.361 ± 0.038
1.16TyrGlu: 1.16 ± 0.032
0.699TyrPhe: 0.699 ± 0.031
1.87TyrGly: 1.87 ± 0.043
0.281TyrHis: 0.281 ± 0.017
0.679TyrIle: 0.679 ± 0.028
0.344TyrLys: 0.344 ± 0.021
2.074TyrLeu: 2.074 ± 0.047
0.26TyrMet: 0.26 ± 0.017
0.437TyrAsn: 0.437 ± 0.022
1.01TyrPro: 1.01 ± 0.033
0.549TyrGln: 0.549 ± 0.021
1.635TyrArg: 1.635 ± 0.042
1.006TyrSer: 1.006 ± 0.032
1.243TyrThr: 1.243 ± 0.044
1.609TyrVal: 1.609 ± 0.042
0.322TyrTrp: 0.322 ± 0.019
0.457TyrTyr: 0.457 ± 0.024
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2917 proteins (977528 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski