Bifidobacterium subtile

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087E8H8|A0A087E8H8_9BIFI Peptide ABC transporter permease OS=Bifidobacterium subtile OX=77635 GN=BISU_1117 PE=3 SV=1
MM1 pKa = 7.13AQDD4 pKa = 3.51YY5 pKa = 10.08DD6 pKa = 3.82SPRR9 pKa = 11.84NKK11 pKa = 10.84DD12 pKa = 3.11EE13 pKa = 5.41DD14 pKa = 3.99EE15 pKa = 4.81EE16 pKa = 4.49SLQEE20 pKa = 4.51LGKK23 pKa = 10.91GSQNSSNEE31 pKa = 3.44IDD33 pKa = 3.9DD34 pKa = 4.83DD35 pKa = 4.4EE36 pKa = 4.41NAIAEE41 pKa = 4.35DD42 pKa = 4.23YY43 pKa = 9.11EE44 pKa = 5.05LPGADD49 pKa = 5.03LSNEE53 pKa = 4.1DD54 pKa = 3.77ASVTVIPMQGDD65 pKa = 3.56EE66 pKa = 5.01FVCSQCFLVRR76 pKa = 11.84HH77 pKa = 6.27RR78 pKa = 11.84SQLAYY83 pKa = 9.44MDD85 pKa = 4.69EE86 pKa = 4.89DD87 pKa = 3.8GQPVCKK93 pKa = 10.23EE94 pKa = 3.94CAAA97 pKa = 4.21

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087E3Z3|A0A087E3Z3_9BIFI DEAD/DEAH box helicase domain-containing protein OS=Bifidobacterium subtile OX=77635 GN=BISU_1694 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.63KK15 pKa = 9.39KK16 pKa = 9.21HH17 pKa = 5.44RR18 pKa = 11.84KK19 pKa = 6.23MLRR22 pKa = 11.84KK23 pKa = 7.88TRR25 pKa = 11.84HH26 pKa = 3.78QRR28 pKa = 11.84KK29 pKa = 8.94

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2259

0

2259

771925

29

3168

341.7

37.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.748 ± 0.069

0.886 ± 0.016

6.274 ± 0.05

5.429 ± 0.053

3.342 ± 0.031

8.197 ± 0.043

2.295 ± 0.024

5.312 ± 0.039

3.393 ± 0.048

9.131 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.519 ± 0.022

3.093 ± 0.03

4.626 ± 0.032

3.762 ± 0.03

6.324 ± 0.056

6.649 ± 0.046

5.662 ± 0.035

7.52 ± 0.038

1.291 ± 0.02

2.547 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski