Amino acid dipepetide frequency for Bifidobacterium subtile

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.441AlaAla: 15.441 ± 0.223	1.17AlaCys: 1.17 ± 0.037	7.327AlaAsp: 7.327 ± 0.121	5.831AlaGlu: 5.831 ± 0.106	3.744AlaPhe: 3.744 ± 0.064	9.729AlaGly: 9.729 ± 0.155	2.53AlaHis: 2.53 ± 0.059	5.969AlaIle: 5.969 ± 0.094	4.459AlaLys: 4.459 ± 0.088	11.556AlaLeu: 11.556 ± 0.14	3.296AlaMet: 3.296 ± 0.076	3.465AlaAsn: 3.465 ± 0.075	4.293AlaPro: 4.293 ± 0.087	5.419AlaGln: 5.419 ± 0.092	6.629AlaArg: 6.629 ± 0.102	7.67AlaSer: 7.67 ± 0.112	5.925AlaThr: 5.925 ± 0.089	8.851AlaVal: 8.851 ± 0.134	1.485AlaTrp: 1.485 ± 0.051	2.692AlaTyr: 2.692 ± 0.06	0.0AlaXaa: 0.0 ± 0.0
Cys	1.093CysAla: 1.093 ± 0.044	0.128CysCys: 0.128 ± 0.016	0.518CysAsp: 0.518 ± 0.026	0.514CysGlu: 0.514 ± 0.026	0.311CysPhe: 0.311 ± 0.019	0.963CysGly: 0.963 ± 0.038	0.205CysHis: 0.205 ± 0.017	0.413CysIle: 0.413 ± 0.02	0.21CysLys: 0.21 ± 0.018	0.751CysLeu: 0.751 ± 0.033	0.19CysMet: 0.19 ± 0.015	0.206CysAsn: 0.206 ± 0.015	0.468CysPro: 0.468 ± 0.027	0.229CysGln: 0.229 ± 0.022	0.525CysArg: 0.525 ± 0.026	0.595CysSer: 0.595 ± 0.031	0.47CysThr: 0.47 ± 0.025	0.731CysVal: 0.731 ± 0.031	0.111CysTrp: 0.111 ± 0.011	0.229CysTyr: 0.229 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	7.989AspAla: 7.989 ± 0.125	0.496AspCys: 0.496 ± 0.027	4.298AspAsp: 4.298 ± 0.086	4.592AspGlu: 4.592 ± 0.092	2.182AspPhe: 2.182 ± 0.053	5.508AspGly: 5.508 ± 0.114	1.272AspHis: 1.272 ± 0.041	3.36AspIle: 3.36 ± 0.069	2.064AspLys: 2.064 ± 0.052	4.893AspLeu: 4.893 ± 0.085	1.615AspMet: 1.615 ± 0.048	1.64AspAsn: 1.64 ± 0.05	3.355AspPro: 3.355 ± 0.083	1.808AspGln: 1.808 ± 0.048	3.322AspArg: 3.322 ± 0.079	3.929AspSer: 3.929 ± 0.087	3.113AspThr: 3.113 ± 0.071	4.608AspVal: 4.608 ± 0.086	0.926AspTrp: 0.926 ± 0.035	1.772AspTyr: 1.772 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.941GluAla: 5.941 ± 0.104	0.431GluCys: 0.431 ± 0.024	2.877GluAsp: 2.877 ± 0.069	2.959GluGlu: 2.959 ± 0.091	1.759GluPhe: 1.759 ± 0.044	3.743GluGly: 3.743 ± 0.076	1.783GluHis: 1.783 ± 0.05	2.663GluIle: 2.663 ± 0.068	1.614GluLys: 1.614 ± 0.055	5.297GluLeu: 5.297 ± 0.106	1.329GluMet: 1.329 ± 0.037	1.762GluAsn: 1.762 ± 0.05	2.641GluPro: 2.641 ± 0.065	2.925GluGln: 2.925 ± 0.079	4.495GluArg: 4.495 ± 0.094	3.62GluSer: 3.62 ± 0.079	2.993GluThr: 2.993 ± 0.069	3.394GluVal: 3.394 ± 0.072	0.566GluTrp: 0.566 ± 0.031	1.5GluTyr: 1.5 ± 0.047	0.0GluXaa: 0.0 ± 0.0
Phe	4.234PheAla: 4.234 ± 0.08	0.316PheCys: 0.316 ± 0.02	2.522PheAsp: 2.522 ± 0.054	1.808PheGlu: 1.808 ± 0.056	1.144PhePhe: 1.144 ± 0.046	3.21PheGly: 3.21 ± 0.078	0.676PheHis: 0.676 ± 0.03	1.72PheIle: 1.72 ± 0.053	0.957PheLys: 0.957 ± 0.042	2.845PheLeu: 2.845 ± 0.071	0.745PheMet: 0.745 ± 0.034	1.176PheAsn: 1.176 ± 0.044	1.358PhePro: 1.358 ± 0.045	0.912PheGln: 0.912 ± 0.038	1.59PheArg: 1.59 ± 0.045	2.369PheSer: 2.369 ± 0.06	2.014PheThr: 2.014 ± 0.054	2.574PheVal: 2.574 ± 0.057	0.456PheTrp: 0.456 ± 0.025	0.797PheTyr: 0.797 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	8.167GlyAla: 8.167 ± 0.115	0.732GlyCys: 0.732 ± 0.035	4.669GlyAsp: 4.669 ± 0.085	4.675GlyGlu: 4.675 ± 0.086	3.24GlyPhe: 3.24 ± 0.066	6.195GlyGly: 6.195 ± 0.102	1.79GlyHis: 1.79 ± 0.047	4.915GlyIle: 4.915 ± 0.084	3.612GlyLys: 3.612 ± 0.076	7.558GlyLeu: 7.558 ± 0.119	2.307GlyMet: 2.307 ± 0.052	2.733GlyAsn: 2.733 ± 0.072	2.732GlyPro: 2.732 ± 0.061	2.815GlyGln: 2.815 ± 0.069	4.969GlyArg: 4.969 ± 0.09	6.012GlySer: 6.012 ± 0.098	4.792GlyThr: 4.792 ± 0.097	6.418GlyVal: 6.418 ± 0.086	1.152GlyTrp: 1.152 ± 0.041	2.489GlyTyr: 2.489 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	2.73HisAla: 2.73 ± 0.051	0.207HisCys: 0.207 ± 0.015	1.842HisAsp: 1.842 ± 0.054	1.407HisGlu: 1.407 ± 0.047	0.653HisPhe: 0.653 ± 0.027	2.119HisGly: 2.119 ± 0.056	0.626HisHis: 0.626 ± 0.032	1.215HisIle: 1.215 ± 0.043	0.525HisLys: 0.525 ± 0.027	1.644HisLeu: 1.644 ± 0.046	0.608HisMet: 0.608 ± 0.032	0.632HisAsn: 0.632 ± 0.032	1.288HisPro: 1.288 ± 0.047	0.693HisGln: 0.693 ± 0.031	1.571HisArg: 1.571 ± 0.048	1.233HisSer: 1.233 ± 0.043	1.185HisThr: 1.185 ± 0.042	1.801HisVal: 1.801 ± 0.054	0.294HisTrp: 0.294 ± 0.018	0.68HisTyr: 0.68 ± 0.03	0.0HisXaa: 0.0 ± 0.0
Ile	7.453IleAla: 7.453 ± 0.112	0.539IleCys: 0.539 ± 0.027	3.802IleAsp: 3.802 ± 0.074	3.214IleGlu: 3.214 ± 0.075	1.525IlePhe: 1.525 ± 0.05	4.829IleGly: 4.829 ± 0.085	1.075IleHis: 1.075 ± 0.042	2.837IleIle: 2.837 ± 0.066	1.459IleLys: 1.459 ± 0.051	3.923IleLeu: 3.923 ± 0.077	1.185IleMet: 1.185 ± 0.041	1.579IleAsn: 1.579 ± 0.05	2.658IlePro: 2.658 ± 0.06	1.245IleGln: 1.245 ± 0.04	2.964IleArg: 2.964 ± 0.061	3.323IleSer: 3.323 ± 0.076	2.955IleThr: 2.955 ± 0.066	4.828IleVal: 4.828 ± 0.093	0.596IleTrp: 0.596 ± 0.029	1.13IleTyr: 1.13 ± 0.038	0.0IleXaa: 0.0 ± 0.0
Lys	4.259LysAla: 4.259 ± 0.084	0.115LysCys: 0.115 ± 0.012	2.115LysAsp: 2.115 ± 0.064	1.824LysGlu: 1.824 ± 0.054	0.785LysPhe: 0.785 ± 0.034	2.552LysGly: 2.552 ± 0.065	0.733LysHis: 0.733 ± 0.027	1.621LysIle: 1.621 ± 0.056	1.397LysLys: 1.397 ± 0.056	2.917LysLeu: 2.917 ± 0.069	0.732LysMet: 0.732 ± 0.03	1.148LysAsn: 1.148 ± 0.045	2.026LysPro: 2.026 ± 0.054	1.426LysGln: 1.426 ± 0.049	2.228LysArg: 2.228 ± 0.057	2.161LysSer: 2.161 ± 0.056	2.35LysThr: 2.35 ± 0.058	2.353LysVal: 2.353 ± 0.064	0.342LysTrp: 0.342 ± 0.022	0.842LysTyr: 0.842 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	11.037LeuAla: 11.037 ± 0.149	0.95LeuCys: 0.95 ± 0.038	6.198LeuAsp: 6.198 ± 0.103	4.272LeuGlu: 4.272 ± 0.087	3.095LeuPhe: 3.095 ± 0.071	7.585LeuGly: 7.585 ± 0.123	2.048LeuHis: 2.048 ± 0.056	4.973LeuIle: 4.973 ± 0.089	3.013LeuLys: 3.013 ± 0.065	8.707LeuLeu: 8.707 ± 0.136	2.158LeuMet: 2.158 ± 0.057	2.805LeuAsn: 2.805 ± 0.058	4.513LeuPro: 4.513 ± 0.078	2.846LeuGln: 2.846 ± 0.072	5.986LeuArg: 5.986 ± 0.087	6.353LeuSer: 6.353 ± 0.091	5.107LeuThr: 5.107 ± 0.085	6.634LeuVal: 6.634 ± 0.113	1.082LeuTrp: 1.082 ± 0.038	1.948LeuTyr: 1.948 ± 0.055	0.0LeuXaa: 0.0 ± 0.0
Met	2.838MetAla: 2.838 ± 0.064	0.2MetCys: 0.2 ± 0.019	1.233MetAsp: 1.233 ± 0.042	0.965MetGlu: 0.965 ± 0.035	0.824MetPhe: 0.824 ± 0.035	1.757MetGly: 1.757 ± 0.05	0.622MetHis: 0.622 ± 0.03	1.273MetIle: 1.273 ± 0.039	0.86MetLys: 0.86 ± 0.033	2.596MetLeu: 2.596 ± 0.066	0.6MetMet: 0.6 ± 0.031	0.996MetAsn: 0.996 ± 0.038	1.522MetPro: 1.522 ± 0.046	0.937MetGln: 0.937 ± 0.033	1.841MetArg: 1.841 ± 0.06	1.876MetSer: 1.876 ± 0.047	1.851MetThr: 1.851 ± 0.046	1.694MetVal: 1.694 ± 0.049	0.247MetTrp: 0.247 ± 0.016	0.455MetTyr: 0.455 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	4.086AsnAla: 4.086 ± 0.073	0.203AsnCys: 0.203 ± 0.017	2.016AsnAsp: 2.016 ± 0.057	1.657AsnGlu: 1.657 ± 0.049	0.852AsnPhe: 0.852 ± 0.034	3.055AsnGly: 3.055 ± 0.063	0.641AsnHis: 0.641 ± 0.03	1.543AsnIle: 1.543 ± 0.054	0.978AsnLys: 0.978 ± 0.038	2.489AsnLeu: 2.489 ± 0.058	0.737AsnMet: 0.737 ± 0.033	0.964AsnAsn: 0.964 ± 0.04	2.044AsnPro: 2.044 ± 0.054	1.06AsnGln: 1.06 ± 0.04	1.816AsnArg: 1.816 ± 0.046	1.671AsnSer: 1.671 ± 0.046	1.726AsnThr: 1.726 ± 0.05	2.207AsnVal: 2.207 ± 0.071	0.39AsnTrp: 0.39 ± 0.025	0.793AsnTyr: 0.793 ± 0.037	0.0AsnXaa: 0.0 ± 0.0
Pro	5.177ProAla: 5.177 ± 0.094	0.347ProCys: 0.347 ± 0.021	3.228ProAsp: 3.228 ± 0.068	3.228ProGlu: 3.228 ± 0.069	1.548ProPhe: 1.548 ± 0.049	3.745ProGly: 3.745 ± 0.07	1.08ProHis: 1.08 ± 0.037	2.258ProIle: 2.258 ± 0.061	1.718ProLys: 1.718 ± 0.045	3.968ProLeu: 3.968 ± 0.071	1.058ProMet: 1.058 ± 0.039	1.384ProAsn: 1.384 ± 0.041	1.362ProPro: 1.362 ± 0.052	2.165ProGln: 2.165 ± 0.061	2.411ProArg: 2.411 ± 0.059	3.179ProSer: 3.179 ± 0.069	2.661ProThr: 2.661 ± 0.058	3.756ProVal: 3.756 ± 0.072	0.64ProTrp: 0.64 ± 0.03	1.345ProTyr: 1.345 ± 0.04	0.0ProXaa: 0.0 ± 0.0
Gln	4.449GlnAla: 4.449 ± 0.088	0.308GlnCys: 0.308 ± 0.019	1.846GlnAsp: 1.846 ± 0.049	1.884GlnGlu: 1.884 ± 0.051	1.099GlnPhe: 1.099 ± 0.04	2.893GlnGly: 2.893 ± 0.06	0.968GlnHis: 0.968 ± 0.037	1.924GlnIle: 1.924 ± 0.045	0.908GlnLys: 0.908 ± 0.035	3.74GlnLeu: 3.74 ± 0.072	0.93GlnMet: 0.93 ± 0.036	1.023GlnAsn: 1.023 ± 0.044	1.897GlnPro: 1.897 ± 0.057	1.849GlnGln: 1.849 ± 0.064	2.954GlnArg: 2.954 ± 0.068	2.539GlnSer: 2.539 ± 0.062	2.141GlnThr: 2.141 ± 0.054	2.565GlnVal: 2.565 ± 0.053	0.627GlnTrp: 0.627 ± 0.025	1.078GlnTyr: 1.078 ± 0.04	0.0GlnXaa: 0.0 ± 0.0
Arg	5.723ArgAla: 5.723 ± 0.097	0.543ArgCys: 0.543 ± 0.027	3.739ArgAsp: 3.739 ± 0.075	3.883ArgGlu: 3.883 ± 0.076	2.377ArgPhe: 2.377 ± 0.058	4.009ArgGly: 4.009 ± 0.084	1.783ArgHis: 1.783 ± 0.051	3.68ArgIle: 3.68 ± 0.069	2.452ArgLys: 2.452 ± 0.053	5.982ArgLeu: 5.982 ± 0.092	1.773ArgMet: 1.773 ± 0.051	2.082ArgAsn: 2.082 ± 0.056	2.68ArgPro: 2.68 ± 0.06	2.627ArgGln: 2.627 ± 0.056	5.331ArgArg: 5.331 ± 0.105	4.011ArgSer: 4.011 ± 0.087	3.515ArgThr: 3.515 ± 0.07	4.206ArgVal: 4.206 ± 0.086	0.868ArgTrp: 0.868 ± 0.036	1.978ArgTyr: 1.978 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	7.698SerAla: 7.698 ± 0.118	0.534SerCys: 0.534 ± 0.029	4.179SerAsp: 4.179 ± 0.076	3.171SerGlu: 3.171 ± 0.07	2.167SerPhe: 2.167 ± 0.061	6.438SerGly: 6.438 ± 0.1	1.53SerHis: 1.53 ± 0.043	3.504SerIle: 3.504 ± 0.073	2.294SerLys: 2.294 ± 0.061	5.683SerLeu: 5.683 ± 0.087	1.691SerMet: 1.691 ± 0.044	2.058SerAsn: 2.058 ± 0.052	2.824SerPro: 2.824 ± 0.06	2.742SerGln: 2.742 ± 0.066	4.017SerArg: 4.017 ± 0.084	4.719SerSer: 4.719 ± 0.098	3.749SerThr: 3.749 ± 0.086	4.876SerVal: 4.876 ± 0.097	0.9SerTrp: 0.9 ± 0.033	1.714SerTyr: 1.714 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	6.503ThrAla: 6.503 ± 0.099	0.447ThrCys: 0.447 ± 0.021	3.103ThrAsp: 3.103 ± 0.067	2.321ThrGlu: 2.321 ± 0.058	1.851ThrPhe: 1.851 ± 0.057	5.019ThrGly: 5.019 ± 0.098	1.236ThrHis: 1.236 ± 0.044	3.29ThrIle: 3.29 ± 0.068	1.865ThrLys: 1.865 ± 0.061	5.573ThrLeu: 5.573 ± 0.086	1.456ThrMet: 1.456 ± 0.046	1.685ThrAsn: 1.685 ± 0.053	3.18ThrPro: 3.18 ± 0.073	2.078ThrGln: 2.078 ± 0.055	3.256ThrArg: 3.256 ± 0.076	3.452ThrSer: 3.452 ± 0.061	3.419ThrThr: 3.419 ± 0.071	4.839ThrVal: 4.839 ± 0.087	0.693ThrTrp: 0.693 ± 0.03	1.349ThrTyr: 1.349 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	8.49ValAla: 8.49 ± 0.123	0.784ValCys: 0.784 ± 0.035	4.753ValAsp: 4.753 ± 0.084	4.06ValGlu: 4.06 ± 0.087	2.831ValPhe: 2.831 ± 0.062	5.389ValGly: 5.389 ± 0.096	1.521ValHis: 1.521 ± 0.046	4.13ValIle: 4.13 ± 0.083	2.378ValLys: 2.378 ± 0.061	7.315ValLeu: 7.315 ± 0.111	1.893ValMet: 1.893 ± 0.051	2.404ValAsn: 2.404 ± 0.066	3.77ValPro: 3.77 ± 0.083	2.312ValGln: 2.312 ± 0.063	4.624ValArg: 4.624 ± 0.077	5.34ValSer: 5.34 ± 0.091	4.533ValThr: 4.533 ± 0.075	6.155ValVal: 6.155 ± 0.114	0.894ValTrp: 0.894 ± 0.037	1.621ValTyr: 1.621 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	1.163TrpAla: 1.163 ± 0.045	0.154TrpCys: 0.154 ± 0.016	0.692TrpAsp: 0.692 ± 0.032	0.526TrpGlu: 0.526 ± 0.027	0.485TrpPhe: 0.485 ± 0.028	0.882TrpGly: 0.882 ± 0.033	0.341TrpHis: 0.341 ± 0.019	0.681TrpIle: 0.681 ± 0.03	0.477TrpLys: 0.477 ± 0.028	1.498TrpLeu: 1.498 ± 0.052	0.371TrpMet: 0.371 ± 0.022	0.527TrpAsn: 0.527 ± 0.031	0.482TrpPro: 0.482 ± 0.023	0.605TrpGln: 0.605 ± 0.027	0.979TrpArg: 0.979 ± 0.036	0.861TrpSer: 0.861 ± 0.036	0.715TrpThr: 0.715 ± 0.031	0.827TrpVal: 0.827 ± 0.03	0.277TrpTrp: 0.277 ± 0.019	0.364TrpTyr: 0.364 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.009TyrAla: 3.009 ± 0.066	0.257TyrCys: 0.257 ± 0.02	1.786TyrAsp: 1.786 ± 0.055	1.5TyrGlu: 1.5 ± 0.051	0.953TyrPhe: 0.953 ± 0.036	2.353TyrGly: 2.353 ± 0.063	0.494TyrHis: 0.494 ± 0.029	1.146TyrIle: 1.146 ± 0.04	0.687TyrLys: 0.687 ± 0.03	2.376TyrLeu: 2.376 ± 0.06	0.512TyrMet: 0.512 ± 0.026	0.657TyrAsn: 0.657 ± 0.031	1.163TyrPro: 1.163 ± 0.04	0.926TyrGln: 0.926 ± 0.035	1.736TyrArg: 1.736 ± 0.054	1.577TyrSer: 1.577 ± 0.045	1.402TyrThr: 1.402 ± 0.047	1.881TyrVal: 1.881 ± 0.052	0.36TyrTrp: 0.36 ± 0.023	0.694TyrTyr: 0.694 ± 0.029	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2259 proteins (771925 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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