Alkalitalea saponilacus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Marinilabiliales; Marinilabiliaceae; Alkalitalea

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3564 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5HMG7|A0A1T5HMG7_9BACT Gliding motility-associated protein GldL OS=Alkalitalea saponilacus OX=889453 GN=SAMN03080601_02461 PE=4 SV=1
MM1 pKa = 7.69KK2 pKa = 10.65YY3 pKa = 10.05KK4 pKa = 10.45VLSIALLTFVLTSCYY19 pKa = 10.1DD20 pKa = 3.14RR21 pKa = 11.84EE22 pKa = 4.2YY23 pKa = 10.77FQLAEE28 pKa = 4.21TVQPVLSSSEE38 pKa = 4.4SISDD42 pKa = 3.62VVIEE46 pKa = 5.07EE47 pKa = 4.64DD48 pKa = 4.8ALDD51 pKa = 3.5QNFTVFSWTAASFGDD66 pKa = 3.85GLPDD70 pKa = 3.19AEE72 pKa = 4.49YY73 pKa = 11.2ALRR76 pKa = 11.84MDD78 pKa = 4.4LAGNNFQNPISLGRR92 pKa = 11.84TKK94 pKa = 10.66SLSISVLNSRR104 pKa = 11.84MNQNLLSIGIEE115 pKa = 3.97PGNPASIEE123 pKa = 4.21FQLHH127 pKa = 5.89ASINPDD133 pKa = 2.45VSTISNIVTSTVTPLEE149 pKa = 4.08VVIEE153 pKa = 4.16YY154 pKa = 7.44PTIYY158 pKa = 10.53LAGDD162 pKa = 3.4HH163 pKa = 6.54NGWSFNDD170 pKa = 3.13QLYY173 pKa = 11.32SMRR176 pKa = 11.84SDD178 pKa = 3.9EE179 pKa = 4.23IYY181 pKa = 10.89EE182 pKa = 4.1GFIYY186 pKa = 9.78MDD188 pKa = 4.9NGDD191 pKa = 3.19NWIGFKK197 pKa = 9.91MSYY200 pKa = 9.54EE201 pKa = 4.09PDD203 pKa = 3.48WIEE206 pKa = 4.58DD207 pKa = 3.86LVIGDD212 pKa = 4.85PDD214 pKa = 3.85DD215 pKa = 4.82SGTSGTLQVGNWGGSNIYY233 pKa = 8.85ATEE236 pKa = 4.26GPGYY240 pKa = 10.43YY241 pKa = 9.73RR242 pKa = 11.84IRR244 pKa = 11.84ANLVNNTISILKK256 pKa = 8.09TEE258 pKa = 4.07WAITGDD264 pKa = 3.61FNGWSFTPLVYY275 pKa = 9.83DD276 pKa = 3.69TDD278 pKa = 4.17DD279 pKa = 4.12KK280 pKa = 11.57VWSATIDD287 pKa = 3.34MTAGGFKK294 pKa = 10.56FIANEE299 pKa = 3.73DD300 pKa = 3.22WSLVFGDD307 pKa = 4.69DD308 pKa = 4.61DD309 pKa = 5.51LNGVLDD315 pKa = 4.95PGRR318 pKa = 11.84DD319 pKa = 3.64DD320 pKa = 4.75NNIQITEE327 pKa = 4.32DD328 pKa = 3.32GNYY331 pKa = 9.28TIIMDD336 pKa = 4.49LSGAPYY342 pKa = 10.12VYY344 pKa = 10.53SIVKK348 pKa = 9.73NN349 pKa = 3.77

Molecular weight: 38.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5HHU8|A0A1T5HHU8_9BACT Uncharacterized protein OS=Alkalitalea saponilacus OX=889453 GN=SAMN03080601_02328 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.89RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.98HH16 pKa = 3.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MATANGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.77KK41 pKa = 10.08LTVSDD46 pKa = 3.89EE47 pKa = 4.47RR48 pKa = 11.84KK49 pKa = 10.13HH50 pKa = 5.97KK51 pKa = 10.83AA52 pKa = 3.04

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3564

0

3564

1322236

39

6369

371.0

41.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.208 ± 0.04

0.786 ± 0.016

5.514 ± 0.033

6.775 ± 0.039

5.218 ± 0.029

6.853 ± 0.052

2.098 ± 0.017

7.733 ± 0.035

5.824 ± 0.063

9.128 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.52 ± 0.021

5.796 ± 0.037

3.843 ± 0.024

3.356 ± 0.021

4.709 ± 0.03

6.68 ± 0.031

5.263 ± 0.04

6.495 ± 0.037

1.259 ± 0.014

3.944 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski