Streptomyces radiopugnans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5419 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9I9P1|A0A1H9I9P1_9ACTN DNA-binding response regulator NarL/FixJ family contains REC and HTH domains OS=Streptomyces radiopugnans OX=403935 GN=SAMN05216481_11390 PE=4 SV=1
MM1 pKa = 7.2SQPPPGQPPHH11 pKa = 7.33DD12 pKa = 4.11GFGPPQYY19 pKa = 10.25PGGQPQQPQQPPQQPPPPQGGPPQAPPPGYY49 pKa = 9.69GGPAAYY55 pKa = 8.58PQGGQPGYY63 pKa = 10.81AYY65 pKa = 7.36PQPGQQPPQQPYY77 pKa = 9.41GQPNPYY83 pKa = 9.82AAYY86 pKa = 8.82PPPPQAGGGSGPKK99 pKa = 8.62GTQGRR104 pKa = 11.84VALIVGGAVVALALIGGGVYY124 pKa = 10.4LAVGGDD130 pKa = 3.89DD131 pKa = 4.33GEE133 pKa = 4.61PAATAASPSPGVSSGPAEE151 pKa = 4.14EE152 pKa = 4.11TDD154 pKa = 3.64EE155 pKa = 4.61PEE157 pKa = 4.37EE158 pKa = 4.02PTEE161 pKa = 4.53EE162 pKa = 4.14PTEE165 pKa = 4.57DD166 pKa = 3.62PLGDD170 pKa = 3.55YY171 pKa = 10.74DD172 pKa = 4.6EE173 pKa = 5.14PTDD176 pKa = 3.93EE177 pKa = 5.19PVDD180 pKa = 4.18EE181 pKa = 4.78PAGAPPAQNDD191 pKa = 5.07FKK193 pKa = 11.39GQWQGEE199 pKa = 4.58GARR202 pKa = 11.84TLTVGKK208 pKa = 10.24VFEE211 pKa = 4.49SGEE214 pKa = 4.21AEE216 pKa = 4.22GKK218 pKa = 10.52NSVSWIEE225 pKa = 3.76FGGDD229 pKa = 4.33GICVGIGEE237 pKa = 4.37NQSRR241 pKa = 11.84GYY243 pKa = 10.52RR244 pKa = 11.84IALKK248 pKa = 10.42CGEE251 pKa = 4.71GDD253 pKa = 3.64DD254 pKa = 4.3EE255 pKa = 4.79EE256 pKa = 5.73YY257 pKa = 10.51IAGTASKK264 pKa = 9.31TSDD267 pKa = 3.4GEE269 pKa = 4.42SIKK272 pKa = 10.77IKK274 pKa = 9.98WDD276 pKa = 3.66DD277 pKa = 3.8GGGTDD282 pKa = 3.41TLDD285 pKa = 3.44WIGDD289 pKa = 3.92DD290 pKa = 3.91NVV292 pKa = 3.23

Molecular weight: 29.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9CHS2|A0A1H9CHS2_9ACTN Protein SCO1/2 OS=Streptomyces radiopugnans OX=403935 GN=SAMN05216481_103249 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5419

0

5419

1754213

30

2920

323.7

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.868 ± 0.059

0.811 ± 0.01

5.835 ± 0.024

6.317 ± 0.033

2.546 ± 0.019

10.194 ± 0.037

2.248 ± 0.017

2.78 ± 0.022

1.841 ± 0.026

10.095 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.695 ± 0.014

1.611 ± 0.02

6.4 ± 0.035

2.386 ± 0.021

8.896 ± 0.047

4.799 ± 0.024

5.791 ± 0.038

8.408 ± 0.031

1.475 ± 0.015

2.003 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski