Amino acid dipepetide frequency for Streptomyces radiopugnans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.903AlaAla: 22.903 ± 0.199	1.095AlaCys: 1.095 ± 0.026	8.383AlaAsp: 8.383 ± 0.079	9.7AlaGlu: 9.7 ± 0.113	3.294AlaPhe: 3.294 ± 0.048	14.734AlaGly: 14.734 ± 0.113	2.759AlaHis: 2.759 ± 0.042	2.924AlaIle: 2.924 ± 0.05	2.351AlaLys: 2.351 ± 0.046	14.331AlaLeu: 14.331 ± 0.112	2.435AlaMet: 2.435 ± 0.038	1.723AlaAsn: 1.723 ± 0.033	7.835AlaPro: 7.835 ± 0.103	3.208AlaGln: 3.208 ± 0.058	11.688AlaArg: 11.688 ± 0.11	5.769AlaSer: 5.769 ± 0.065	6.092AlaThr: 6.092 ± 0.064	12.983AlaVal: 12.983 ± 0.106	1.814AlaTrp: 1.814 ± 0.034	2.662AlaTyr: 2.662 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	1.132CysAla: 1.132 ± 0.024	0.101CysCys: 0.101 ± 0.009	0.513CysAsp: 0.513 ± 0.02	0.465CysGlu: 0.465 ± 0.018	0.218CysPhe: 0.218 ± 0.012	1.056CysGly: 1.056 ± 0.027	0.199CysHis: 0.199 ± 0.011	0.129CysIle: 0.129 ± 0.009	0.082CysLys: 0.082 ± 0.007	0.754CysLeu: 0.754 ± 0.019	0.123CysMet: 0.123 ± 0.008	0.119CysAsn: 0.119 ± 0.008	0.528CysPro: 0.528 ± 0.019	0.154CysGln: 0.154 ± 0.01	0.697CysArg: 0.697 ± 0.021	0.416CysSer: 0.416 ± 0.015	0.474CysThr: 0.474 ± 0.017	0.689CysVal: 0.689 ± 0.02	0.125CysTrp: 0.125 ± 0.009	0.133CysTyr: 0.133 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	7.478AspAla: 7.478 ± 0.076	0.438AspCys: 0.438 ± 0.017	3.266AspAsp: 3.266 ± 0.054	3.891AspGlu: 3.891 ± 0.055	1.571AspPhe: 1.571 ± 0.034	7.015AspGly: 7.015 ± 0.073	1.376AspHis: 1.376 ± 0.027	1.661AspIle: 1.661 ± 0.032	0.942AspLys: 0.942 ± 0.03	5.843AspLeu: 5.843 ± 0.069	0.815AspMet: 0.815 ± 0.021	0.859AspAsn: 0.859 ± 0.023	4.696AspPro: 4.696 ± 0.064	1.14AspGln: 1.14 ± 0.028	5.363AspArg: 5.363 ± 0.057	2.404AspSer: 2.404 ± 0.034	3.165AspThr: 3.165 ± 0.046	4.387AspVal: 4.387 ± 0.055	0.97AspTrp: 0.97 ± 0.025	1.07AspTyr: 1.07 ± 0.023	0.0AspXaa: 0.0 ± 0.0
Glu	8.303GluAla: 8.303 ± 0.096	0.431GluCys: 0.431 ± 0.018	3.245GluAsp: 3.245 ± 0.04	4.693GluGlu: 4.693 ± 0.072	1.433GluPhe: 1.433 ± 0.031	5.174GluGly: 5.174 ± 0.058	1.643GluHis: 1.643 ± 0.031	2.264GluIle: 2.264 ± 0.037	1.569GluLys: 1.569 ± 0.042	7.117GluLeu: 7.117 ± 0.079	0.955GluMet: 0.955 ± 0.023	1.048GluAsn: 1.048 ± 0.024	4.048GluPro: 4.048 ± 0.048	2.104GluGln: 2.104 ± 0.042	6.659GluArg: 6.659 ± 0.07	2.595GluSer: 2.595 ± 0.04	3.028GluThr: 3.028 ± 0.049	4.861GluVal: 4.861 ± 0.06	0.755GluTrp: 0.755 ± 0.022	1.242GluTyr: 1.242 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	3.442PheAla: 3.442 ± 0.045	0.257PheCys: 0.257 ± 0.012	1.799PheAsp: 1.799 ± 0.033	1.41PheGlu: 1.41 ± 0.034	0.811PhePhe: 0.811 ± 0.024	2.848PheGly: 2.848 ± 0.045	0.605PheHis: 0.605 ± 0.017	0.565PheIle: 0.565 ± 0.018	0.431PheLys: 0.431 ± 0.017	2.419PheLeu: 2.419 ± 0.046	0.377PheMet: 0.377 ± 0.015	0.495PheAsn: 0.495 ± 0.015	1.332PhePro: 1.332 ± 0.028	0.587PheGln: 0.587 ± 0.018	1.866PheArg: 1.866 ± 0.031	1.355PheSer: 1.355 ± 0.028	1.943PheThr: 1.943 ± 0.034	2.016PheVal: 2.016 ± 0.036	0.404PheTrp: 0.404 ± 0.017	0.499PheTyr: 0.499 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	12.34GlyAla: 12.34 ± 0.105	0.896GlyCys: 0.896 ± 0.024	5.662GlyAsp: 5.662 ± 0.067	6.615GlyGlu: 6.615 ± 0.068	2.766GlyPhe: 2.766 ± 0.038	11.061GlyGly: 11.061 ± 0.118	2.428GlyHis: 2.428 ± 0.038	3.131GlyIle: 3.131 ± 0.039	2.174GlyLys: 2.174 ± 0.049	9.442GlyLeu: 9.442 ± 0.076	2.085GlyMet: 2.085 ± 0.038	1.709GlyAsn: 1.709 ± 0.043	6.076GlyPro: 6.076 ± 0.08	2.524GlyGln: 2.524 ± 0.045	9.41GlyArg: 9.41 ± 0.084	5.443GlySer: 5.443 ± 0.063	6.655GlyThr: 6.655 ± 0.073	7.61GlyVal: 7.61 ± 0.071	1.706GlyTrp: 1.706 ± 0.036	2.201GlyTyr: 2.201 ± 0.037	0.0GlyXaa: 0.0 ± 0.0
His	2.537HisAla: 2.537 ± 0.042	0.221HisCys: 0.221 ± 0.011	1.271HisAsp: 1.271 ± 0.031	1.134HisGlu: 1.134 ± 0.029	0.578HisPhe: 0.578 ± 0.018	2.565HisGly: 2.565 ± 0.039	0.707HisHis: 0.707 ± 0.022	0.624HisIle: 0.624 ± 0.018	0.288HisLys: 0.288 ± 0.014	2.336HisLeu: 2.336 ± 0.041	0.318HisMet: 0.318 ± 0.013	0.377HisAsn: 0.377 ± 0.015	1.818HisPro: 1.818 ± 0.033	0.56HisGln: 0.56 ± 0.021	2.374HisArg: 2.374 ± 0.039	0.981HisSer: 0.981 ± 0.025	1.364HisThr: 1.364 ± 0.027	1.618HisVal: 1.618 ± 0.03	0.351HisTrp: 0.351 ± 0.013	0.459HisTyr: 0.459 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	4.354IleAla: 4.354 ± 0.066	0.228IleCys: 0.228 ± 0.01	2.007IleAsp: 2.007 ± 0.037	1.812IleGlu: 1.812 ± 0.039	0.562IlePhe: 0.562 ± 0.022	3.202IleGly: 3.202 ± 0.046	0.543IleHis: 0.543 ± 0.018	0.719IleIle: 0.719 ± 0.026	0.538IleLys: 0.538 ± 0.021	2.065IleLeu: 2.065 ± 0.037	0.397IleMet: 0.397 ± 0.015	0.56IleAsn: 0.56 ± 0.019	1.593IlePro: 1.593 ± 0.031	0.544IleGln: 0.544 ± 0.018	2.109IleArg: 2.109 ± 0.04	1.362IleSer: 1.362 ± 0.03	2.05IleThr: 2.05 ± 0.032	2.432IleVal: 2.432 ± 0.041	0.301IleTrp: 0.301 ± 0.014	0.426IleTyr: 0.426 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.497LysAla: 2.497 ± 0.048	0.105LysCys: 0.105 ± 0.008	1.093LysAsp: 1.093 ± 0.027	1.144LysGlu: 1.144 ± 0.027	0.366LysPhe: 0.366 ± 0.015	1.626LysGly: 1.626 ± 0.035	0.365LysHis: 0.365 ± 0.013	0.687LysIle: 0.687 ± 0.023	0.699LysLys: 0.699 ± 0.031	1.751LysLeu: 1.751 ± 0.039	0.308LysMet: 0.308 ± 0.014	0.438LysAsn: 0.438 ± 0.019	1.176LysPro: 1.176 ± 0.028	0.547LysGln: 0.547 ± 0.02	1.321LysArg: 1.321 ± 0.031	0.91LysSer: 0.91 ± 0.028	1.11LysThr: 1.11 ± 0.035	1.645LysVal: 1.645 ± 0.038	0.221LysTrp: 0.221 ± 0.013	0.397LysTyr: 0.397 ± 0.017	0.0LysXaa: 0.0 ± 0.0
Leu	15.173LeuAla: 15.173 ± 0.119	0.88LeuCys: 0.88 ± 0.024	6.346LeuAsp: 6.346 ± 0.066	5.371LeuGlu: 5.371 ± 0.065	2.403LeuPhe: 2.403 ± 0.037	9.202LeuGly: 9.202 ± 0.086	2.17LeuHis: 2.17 ± 0.039	2.655LeuIle: 2.655 ± 0.051	1.692LeuLys: 1.692 ± 0.038	11.345LeuLeu: 11.345 ± 0.126	1.533LeuMet: 1.533 ± 0.031	1.474LeuAsn: 1.474 ± 0.029	6.597LeuPro: 6.597 ± 0.066	1.81LeuGln: 1.81 ± 0.037	9.365LeuArg: 9.365 ± 0.086	4.954LeuSer: 4.954 ± 0.054	6.292LeuThr: 6.292 ± 0.067	8.6LeuVal: 8.6 ± 0.088	1.284LeuTrp: 1.284 ± 0.027	1.806LeuTyr: 1.806 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.268MetAla: 2.268 ± 0.039	0.132MetCys: 0.132 ± 0.008	0.872MetAsp: 0.872 ± 0.024	0.854MetGlu: 0.854 ± 0.021	0.417MetPhe: 0.417 ± 0.017	1.35MetGly: 1.35 ± 0.031	0.332MetHis: 0.332 ± 0.013	0.554MetIle: 0.554 ± 0.019	0.363MetLys: 0.363 ± 0.015	1.597MetLeu: 1.597 ± 0.032	0.271MetMet: 0.271 ± 0.014	0.384MetAsn: 0.384 ± 0.016	1.129MetPro: 1.129 ± 0.024	0.359MetGln: 0.359 ± 0.016	1.549MetArg: 1.549 ± 0.029	1.279MetSer: 1.279 ± 0.027	1.467MetThr: 1.467 ± 0.031	1.256MetVal: 1.256 ± 0.029	0.214MetTrp: 0.214 ± 0.011	0.303MetTyr: 0.303 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.001AsnAla: 2.001 ± 0.042	0.152AsnCys: 0.152 ± 0.01	0.854AsnAsp: 0.854 ± 0.024	0.795AsnGlu: 0.795 ± 0.021	0.438AsnPhe: 0.438 ± 0.017	1.72AsnGly: 1.72 ± 0.035	0.395AsnHis: 0.395 ± 0.016	0.562AsnIle: 0.562 ± 0.019	0.335AsnLys: 0.335 ± 0.016	1.494AsnLeu: 1.494 ± 0.031	0.279AsnMet: 0.279 ± 0.013	0.372AsnAsn: 0.372 ± 0.016	1.298AsnPro: 1.298 ± 0.029	0.44AsnGln: 0.44 ± 0.015	1.255AsnArg: 1.255 ± 0.03	0.782AsnSer: 0.782 ± 0.022	1.053AsnThr: 1.053 ± 0.03	1.27AsnVal: 1.27 ± 0.026	0.273AsnTrp: 0.273 ± 0.012	0.348AsnTyr: 0.348 ± 0.014	0.001AsnXaa: 0.001 ± 0.001
Pro	9.497ProAla: 9.497 ± 0.116	0.357ProCys: 0.357 ± 0.015	4.567ProAsp: 4.567 ± 0.051	4.963ProGlu: 4.963 ± 0.056	1.437ProPhe: 1.437 ± 0.029	8.036ProGly: 8.036 ± 0.096	1.398ProHis: 1.398 ± 0.029	1.097ProIle: 1.097 ± 0.026	1.007ProLys: 1.007 ± 0.023	5.335ProLeu: 5.335 ± 0.061	0.984ProMet: 0.984 ± 0.024	0.843ProAsn: 0.843 ± 0.023	4.146ProPro: 4.146 ± 0.073	1.617ProGln: 1.617 ± 0.047	4.671ProArg: 4.671 ± 0.058	3.322ProSer: 3.322 ± 0.062	2.668ProThr: 2.668 ± 0.04	5.734ProVal: 5.734 ± 0.069	0.923ProTrp: 0.923 ± 0.023	1.4ProTyr: 1.4 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.292GlnAla: 3.292 ± 0.051	0.156GlnCys: 0.156 ± 0.009	1.181GlnAsp: 1.181 ± 0.031	1.423GlnGlu: 1.423 ± 0.031	0.54GlnPhe: 0.54 ± 0.019	2.099GlnGly: 2.099 ± 0.039	0.531GlnHis: 0.531 ± 0.023	0.906GlnIle: 0.906 ± 0.025	0.509GlnLys: 0.509 ± 0.02	2.379GlnLeu: 2.379 ± 0.045	0.417GlnMet: 0.417 ± 0.015	0.407GlnAsn: 0.407 ± 0.016	1.531GlnPro: 1.531 ± 0.045	1.007GlnGln: 1.007 ± 0.036	2.226GlnArg: 2.226 ± 0.045	1.051GlnSer: 1.051 ± 0.025	1.22GlnThr: 1.22 ± 0.026	2.06GlnVal: 2.06 ± 0.037	0.42GlnTrp: 0.42 ± 0.016	0.505GlnTyr: 0.505 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	11.065ArgAla: 11.065 ± 0.097	0.685ArgCys: 0.685 ± 0.02	4.57ArgAsp: 4.57 ± 0.056	5.943ArgGlu: 5.943 ± 0.071	2.357ArgPhe: 2.357 ± 0.041	7.019ArgGly: 7.019 ± 0.078	2.245ArgHis: 2.245 ± 0.036	3.27ArgIle: 3.27 ± 0.044	1.602ArgLys: 1.602 ± 0.035	9.662ArgLeu: 9.662 ± 0.09	1.946ArgMet: 1.946 ± 0.037	1.377ArgAsn: 1.377 ± 0.033	5.862ArgPro: 5.862 ± 0.075	2.322ArgGln: 2.322 ± 0.036	9.349ArgArg: 9.349 ± 0.096	4.177ArgSer: 4.177 ± 0.052	5.852ArgThr: 5.852 ± 0.057	6.246ArgVal: 6.246 ± 0.074	1.516ArgTrp: 1.516 ± 0.031	1.893ArgTyr: 1.893 ± 0.032	0.0ArgXaa: 0.0 ± 0.0
Ser	6.639SerAla: 6.639 ± 0.068	0.406SerCys: 0.406 ± 0.016	2.42SerAsp: 2.42 ± 0.044	2.398SerGlu: 2.398 ± 0.03	1.397SerPhe: 1.397 ± 0.031	6.188SerGly: 6.188 ± 0.064	0.977SerHis: 0.977 ± 0.023	1.195SerIle: 1.195 ± 0.03	0.826SerLys: 0.826 ± 0.027	4.53SerLeu: 4.53 ± 0.054	0.955SerMet: 0.955 ± 0.023	0.775SerAsn: 0.775 ± 0.025	3.28SerPro: 3.28 ± 0.046	1.062SerGln: 1.062 ± 0.029	3.82SerArg: 3.82 ± 0.047	2.532SerSer: 2.532 ± 0.049	2.648SerThr: 2.648 ± 0.036	3.991SerVal: 3.991 ± 0.044	0.832SerTrp: 0.832 ± 0.022	1.12SerTyr: 1.12 ± 0.028	0.0SerXaa: 0.0 ± 0.0
Thr	9.123ThrAla: 9.123 ± 0.089	0.373ThrCys: 0.373 ± 0.013	3.372ThrAsp: 3.372 ± 0.049	3.306ThrGlu: 3.306 ± 0.041	1.38ThrPhe: 1.38 ± 0.033	7.273ThrGly: 7.273 ± 0.072	1.035ThrHis: 1.035 ± 0.023	1.43ThrIle: 1.43 ± 0.032	0.87ThrLys: 0.87 ± 0.029	5.106ThrLeu: 5.106 ± 0.057	0.849ThrMet: 0.849 ± 0.023	0.821ThrAsn: 0.821 ± 0.026	3.761ThrPro: 3.761 ± 0.047	1.037ThrGln: 1.037 ± 0.025	3.823ThrArg: 3.823 ± 0.043	2.705ThrSer: 2.705 ± 0.042	3.461ThrThr: 3.461 ± 0.057	6.199ThrVal: 6.199 ± 0.054	0.783ThrTrp: 0.783 ± 0.023	1.201ThrTyr: 1.201 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	10.391ValAla: 10.391 ± 0.084	0.859ValCys: 0.859 ± 0.023	4.647ValAsp: 4.647 ± 0.052	5.123ValGlu: 5.123 ± 0.059	2.416ValPhe: 2.416 ± 0.04	6.36ValGly: 6.36 ± 0.071	2.037ValHis: 2.037 ± 0.036	2.477ValIle: 2.477 ± 0.039	1.48ValLys: 1.48 ± 0.034	9.79ValLeu: 9.79 ± 0.086	1.41ValMet: 1.41 ± 0.029	1.575ValAsn: 1.575 ± 0.031	5.534ValPro: 5.534 ± 0.06	1.79ValGln: 1.79 ± 0.036	8.057ValArg: 8.057 ± 0.08	4.17ValSer: 4.17 ± 0.052	5.211ValThr: 5.211 ± 0.06	7.961ValVal: 7.961 ± 0.089	1.197ValTrp: 1.197 ± 0.027	1.595ValTyr: 1.595 ± 0.031	0.0ValXaa: 0.0 ± 0.0
Trp	1.598TrpAla: 1.598 ± 0.027	0.16TrpCys: 0.16 ± 0.011	0.783TrpAsp: 0.783 ± 0.022	0.846TrpGlu: 0.846 ± 0.021	0.502TrpPhe: 0.502 ± 0.017	1.049TrpGly: 1.049 ± 0.028	0.353TrpHis: 0.353 ± 0.013	0.508TrpIle: 0.508 ± 0.018	0.338TrpLys: 0.338 ± 0.015	1.679TrpLeu: 1.679 ± 0.037	0.262TrpMet: 0.262 ± 0.014	0.383TrpAsn: 0.383 ± 0.017	0.77TrpPro: 0.77 ± 0.023	0.536TrpGln: 0.536 ± 0.021	1.454TrpArg: 1.454 ± 0.037	0.906TrpSer: 0.906 ± 0.023	0.991TrpThr: 0.991 ± 0.028	0.942TrpVal: 0.942 ± 0.024	0.347TrpTrp: 0.347 ± 0.017	0.345TrpTyr: 0.345 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.648TyrAla: 2.648 ± 0.042	0.178TyrCys: 0.178 ± 0.011	1.498TyrAsp: 1.498 ± 0.042	1.284TyrGlu: 1.284 ± 0.033	0.576TyrPhe: 0.576 ± 0.021	2.357TyrGly: 2.357 ± 0.041	0.385TyrHis: 0.385 ± 0.016	0.445TyrIle: 0.445 ± 0.016	0.311TyrLys: 0.311 ± 0.013	1.979TyrLeu: 1.979 ± 0.034	0.227TyrMet: 0.227 ± 0.013	0.377TyrAsn: 0.377 ± 0.018	0.992TyrPro: 0.992 ± 0.023	0.51TyrGln: 0.51 ± 0.019	1.9TyrArg: 1.9 ± 0.036	0.878TyrSer: 0.878 ± 0.025	1.161TyrThr: 1.161 ± 0.028	1.58TyrVal: 1.58 ± 0.031	0.317TyrTrp: 0.317 ± 0.013	0.428TyrTyr: 0.428 ± 0.016	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.002XaaXaa: 0.002 ± 0.002

Statistics based on 5419 proteins (1754213 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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