Garlic virus C
Average proteome isoelectric point is 6.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|O55602|O55602_9VIRU 27kDa protein OS=Garlic virus C OX=12431 PE=4 SV=1
MM1 pKa = 6.78 TTVQKK6 pKa = 11.0 LLDD9 pKa = 4.13 QISDD13 pKa = 3.73 PNARR17 pKa = 11.84 AAYY20 pKa = 9.74 SQEE23 pKa = 4.07 CLSAAKK29 pKa = 10.15 NAATKK34 pKa = 11.03 AMTIAPYY41 pKa = 10.82 AVTQTEE47 pKa = 4.29 ALTLEE52 pKa = 4.81 RR53 pKa = 11.84 IGITTSPFATTSHH66 pKa = 4.89 THH68 pKa = 5.93 AADD71 pKa = 4.1 KK72 pKa = 10.63 IIEE75 pKa = 4.22 NDD77 pKa = 3.35 CLEE80 pKa = 4.92 IISHH84 pKa = 6.31 YY85 pKa = 10.16 LPKK88 pKa = 10.92 DD89 pKa = 3.94 PITLIQLKK97 pKa = 10.09 RR98 pKa = 11.84 SKK100 pKa = 10.81 LHH102 pKa = 5.53 LLKK105 pKa = 10.72 RR106 pKa = 11.84 GPTDD110 pKa = 4.16 DD111 pKa = 5.33 AFQNYY116 pKa = 7.45 CHH118 pKa = 6.57 EE119 pKa = 4.78 PKK121 pKa = 10.52 DD122 pKa = 3.63 VLRR125 pKa = 11.84 FGLTNPHH132 pKa = 6.08 TCPTVTTSCAVISDD146 pKa = 4.03 TLHH149 pKa = 6.41 FMSSQQLWTLFARR162 pKa = 11.84 NPALQRR168 pKa = 11.84 LYY170 pKa = 9.83 GTLVLPVEE178 pKa = 4.86 ALHH181 pKa = 6.49 KK182 pKa = 10.57 LPSLFPEE189 pKa = 4.6 IYY191 pKa = 10.22 KK192 pKa = 10.54 LEE194 pKa = 4.4 YY195 pKa = 9.26 YY196 pKa = 10.04 DD197 pKa = 3.87 KK198 pKa = 10.74 HH199 pKa = 5.5 FAYY202 pKa = 10.0 MPGGHH207 pKa = 6.74 GGGAYY212 pKa = 8.55 IHH214 pKa = 7.06 SYY216 pKa = 8.85 GTLKK220 pKa = 10.24 WLSTAQIGSGLNHH233 pKa = 6.82 LSIEE237 pKa = 4.31 KK238 pKa = 10.16 IEE240 pKa = 4.33 TKK242 pKa = 10.04 AAHH245 pKa = 6.97 HH246 pKa = 6.42 IMVIQRR252 pKa = 11.84 RR253 pKa = 11.84 RR254 pKa = 11.84 PEE256 pKa = 4.51 LPWPLPPVWVYY267 pKa = 10.41 HH268 pKa = 6.16 ASDD271 pKa = 3.93 YY272 pKa = 11.61 VKK274 pKa = 10.48 LPEE277 pKa = 4.04 IFYY280 pKa = 8.66 PTEE283 pKa = 4.03 ANVQRR288 pKa = 11.84 PYY290 pKa = 10.24 PHH292 pKa = 6.9 VFIKK296 pKa = 10.55 RR297 pKa = 11.84 LQLYY301 pKa = 8.31 CFSVKK306 pKa = 10.2 AVSLRR311 pKa = 11.84 DD312 pKa = 3.18 IFAKK316 pKa = 10.0 IRR318 pKa = 11.84 QVLPTEE324 pKa = 3.8 EE325 pKa = 4.22 LARR328 pKa = 11.84 FSTADD333 pKa = 4.67 LIRR336 pKa = 11.84 LANYY340 pKa = 10.44 LLFITGMQQTSDD352 pKa = 3.74 YY353 pKa = 10.41 QSPLMEE359 pKa = 4.49 SLFGKK364 pKa = 10.24 LCLSIRR370 pKa = 11.84 ARR372 pKa = 11.84 IRR374 pKa = 11.84 EE375 pKa = 4.02 FFQNLMGKK383 pKa = 9.59 SSYY386 pKa = 10.93 AALLTVTDD394 pKa = 3.3 VHH396 pKa = 6.14 PVYY399 pKa = 9.79 FTTKK403 pKa = 8.83 PLRR406 pKa = 11.84 RR407 pKa = 11.84 PICGEE412 pKa = 3.47 QWFEE416 pKa = 4.04 AEE418 pKa = 4.39 EE419 pKa = 4.45 AEE421 pKa = 4.47 EE422 pKa = 4.69 DD423 pKa = 3.9 PSVPSPTGYY432 pKa = 9.88 QRR434 pKa = 11.84 PSPDD438 pKa = 4.31 DD439 pKa = 4.18 LDD441 pKa = 4.24 LLAEE445 pKa = 4.56 FEE447 pKa = 4.36 QQNLLNGAEE456 pKa = 4.43 GQRR459 pKa = 11.84 EE460 pKa = 4.08 RR461 pKa = 11.84 QGEE464 pKa = 4.21 HH465 pKa = 5.34 AHH467 pKa = 6.77 GPNQNQTTSTQPPPHH482 pKa = 6.39 QPSPHH487 pKa = 7.03 DD488 pKa = 3.76 AAQSGSSSEE497 pKa = 3.91 ARR499 pKa = 11.84 KK500 pKa = 8.01 TEE502 pKa = 4.03 SSDD505 pKa = 3.53 EE506 pKa = 4.34 PEE508 pKa = 4.38 VSCKK512 pKa = 10.6 DD513 pKa = 3.66 EE514 pKa = 4.33 EE515 pKa = 4.47 DD516 pKa = 4.07 LGSGTSDD523 pKa = 2.88 GHH525 pKa = 4.97 VTDD528 pKa = 4.4 WSDD531 pKa = 4.19 SEE533 pKa = 4.96 SDD535 pKa = 4.23 CSDD538 pKa = 4.03 DD539 pKa = 4.12 KK540 pKa = 11.34 HH541 pKa = 7.81 DD542 pKa = 5.02 QIFDD546 pKa = 3.78 SLSLFDD552 pKa = 4.38 TADD555 pKa = 3.74 LPDD558 pKa = 4.29 LEE560 pKa = 4.69 EE561 pKa = 4.58 VEE563 pKa = 4.7 EE564 pKa = 4.56 EE565 pKa = 3.9 PDD567 pKa = 3.77 AAHH570 pKa = 5.95 TTQTPTPDD578 pKa = 3.25 VTDD581 pKa = 3.59 EE582 pKa = 4.21 SSAVQHH588 pKa = 5.8 TQTPHH593 pKa = 5.83 IAEE596 pKa = 4.43 SLVDD600 pKa = 3.4 SDD602 pKa = 4.31 VFVGEE607 pKa = 4.56 TIDD610 pKa = 6.56 DD611 pKa = 3.75 EE612 pKa = 4.67 TQQPDD617 pKa = 3.9 TVPAVQTRR625 pKa = 11.84 EE626 pKa = 3.95 NSRR629 pKa = 11.84 STTVALEE636 pKa = 4.24 PFDD639 pKa = 4.48 EE640 pKa = 4.31 EE641 pKa = 4.28 TIEE644 pKa = 3.75 ILKK647 pKa = 9.99 IHH649 pKa = 6.84 GFTNLKK655 pKa = 8.41 PQHH658 pKa = 6.7 DD659 pKa = 4.86 GEE661 pKa = 4.3 FQIAPVFYY669 pKa = 10.83 NRR671 pKa = 11.84 LAKK674 pKa = 10.21 HH675 pKa = 5.41 IPTYY679 pKa = 10.74 EE680 pKa = 4.88 GITTTTQSQFIAMAQALKK698 pKa = 10.37 RR699 pKa = 11.84 NCYY702 pKa = 9.71 RR703 pKa = 11.84 YY704 pKa = 10.6 KK705 pKa = 10.77 LDD707 pKa = 3.98 AKK709 pKa = 10.41 RR710 pKa = 11.84 ATAFMSDD717 pKa = 3.27 VKK719 pKa = 11.38 NNLTGLVLPKK729 pKa = 10.11 LDD731 pKa = 4.63 RR732 pKa = 11.84 DD733 pKa = 4.2 LLTSWVALAEE743 pKa = 4.05 NAKK746 pKa = 10.21 RR747 pKa = 11.84 SVGLIVIHH755 pKa = 6.3 GAGGAGKK762 pKa = 10.07 SRR764 pKa = 11.84 ALQEE768 pKa = 4.13 LLRR771 pKa = 11.84 HH772 pKa = 5.7 GKK774 pKa = 8.9 MDD776 pKa = 3.46 INEE779 pKa = 4.03 VNIVVPTINLAVDD792 pKa = 4.23 WKK794 pKa = 10.8 RR795 pKa = 11.84 KK796 pKa = 8.97 LPSMDD801 pKa = 3.37 PRR803 pKa = 11.84 RR804 pKa = 11.84 IMTFEE809 pKa = 4.01 KK810 pKa = 10.17 ACEE813 pKa = 4.0 RR814 pKa = 11.84 EE815 pKa = 4.35 GKK817 pKa = 10.43 SIVIMDD823 pKa = 5.07 DD824 pKa = 3.38 YY825 pKa = 11.82 GKK827 pKa = 10.9 LPAGYY832 pKa = 8.89 IDD834 pKa = 5.1 AYY836 pKa = 10.95 LAIKK840 pKa = 10.13 FNVEE844 pKa = 3.83 LVILTGDD851 pKa = 3.18 QRR853 pKa = 11.84 QSVFHH858 pKa = 6.42 NDD860 pKa = 2.92 KK861 pKa = 10.46 RR862 pKa = 11.84 DD863 pKa = 3.58 SQIALLNSNTDD874 pKa = 2.73 HH875 pKa = 6.61 YY876 pKa = 11.5 KK877 pKa = 10.46 RR878 pKa = 11.84 YY879 pKa = 9.8 CDD881 pKa = 3.63 YY882 pKa = 11.08 YY883 pKa = 11.51 LNATHH888 pKa = 6.81 RR889 pKa = 11.84 QPRR892 pKa = 11.84 RR893 pKa = 11.84 LANPIKK899 pKa = 9.76 VHH901 pKa = 6.47 AEE903 pKa = 3.97 RR904 pKa = 11.84 EE905 pKa = 3.95 VGGAVKK911 pKa = 10.02 HH912 pKa = 5.55 ATLIPTNAMTLVPAFRR928 pKa = 11.84 SQSLLTDD935 pKa = 4.09 LGRR938 pKa = 11.84 QAMTYY943 pKa = 10.18 AGCQGLTLPHH953 pKa = 6.63 LSIVLDD959 pKa = 4.06 KK960 pKa = 10.18 DD961 pKa = 3.66 TPLCSDD967 pKa = 3.5 EE968 pKa = 4.38 VLYY971 pKa = 9.62 TALSRR976 pKa = 11.84 ASEE979 pKa = 4.43 TITFINTHH987 pKa = 5.35 SNNKK991 pKa = 9.26 EE992 pKa = 3.81 FLDD995 pKa = 4.21 KK996 pKa = 11.06 LDD998 pKa = 3.52 STPYY1002 pKa = 10.72 LKK1004 pKa = 9.81 TLISGVRR1011 pKa = 11.84 EE1012 pKa = 3.87 DD1013 pKa = 3.71 EE1014 pKa = 4.18 QAGRR1018 pKa = 11.84 EE1019 pKa = 4.04 QPPPEE1024 pKa = 4.38 PSVRR1028 pKa = 11.84 DD1029 pKa = 3.9 SPTKK1033 pKa = 8.89 THH1035 pKa = 6.62 IPVANDD1041 pKa = 3.14 SVQLEE1046 pKa = 4.34 GKK1048 pKa = 9.66 IEE1050 pKa = 3.96 AMEE1053 pKa = 4.77 DD1054 pKa = 3.08 KK1055 pKa = 9.95 DD1056 pKa = 4.96 TRR1058 pKa = 11.84 EE1059 pKa = 3.87 LWSGEE1064 pKa = 3.84 EE1065 pKa = 4.18 KK1066 pKa = 10.4 TNLMQTQDD1074 pKa = 3.55 PVVQLFPHH1082 pKa = 5.46 QQAKK1086 pKa = 10.89 DD1087 pKa = 3.24 EE1088 pKa = 4.47 ALFKK1092 pKa = 10.13 ITINEE1097 pKa = 4.64 RR1098 pKa = 11.84 IRR1100 pKa = 11.84 LATPEE1105 pKa = 4.23 TNRR1108 pKa = 11.84 KK1109 pKa = 9.3 ALTDD1113 pKa = 3.54 TLNAGDD1119 pKa = 5.14 LLFEE1123 pKa = 5.19 AYY1125 pKa = 10.18 ASFMNVPKK1133 pKa = 9.48 EE1134 pKa = 3.95 TQAFDD1139 pKa = 3.18 KK1140 pKa = 10.82 RR1141 pKa = 11.84 LWAHH1145 pKa = 6.37 CRR1147 pKa = 11.84 QLALRR1152 pKa = 11.84 TYY1154 pKa = 10.52 LSKK1157 pKa = 9.7 PTANLQQGAKK1167 pKa = 9.87 RR1168 pKa = 11.84 QDD1170 pKa = 3.13 PDD1172 pKa = 3.57 FPEE1175 pKa = 3.86 NAIALFNKK1183 pKa = 8.34 SQWVKK1188 pKa = 10.35 KK1189 pKa = 10.15 LEE1191 pKa = 4.14 KK1192 pKa = 10.19 VGCRR1196 pKa = 11.84 FKK1198 pKa = 11.1 AGQTISAFKK1207 pKa = 10.55 QSTVLLTTTMALYY1220 pKa = 10.13 LRR1222 pKa = 11.84 KK1223 pKa = 9.51 KK1224 pKa = 10.63 RR1225 pKa = 11.84 EE1226 pKa = 3.53 GHH1228 pKa = 5.5 QPDD1231 pKa = 3.85 NVFIMCEE1238 pKa = 3.76 KK1239 pKa = 10.26 TPEE1242 pKa = 4.03 QFNSFVLTKK1251 pKa = 10.0 WDD1253 pKa = 3.81 FTRR1256 pKa = 11.84 PNYY1259 pKa = 9.75 TSDD1262 pKa = 3.13 YY1263 pKa = 8.03 TQYY1266 pKa = 11.28 DD1267 pKa = 3.62 QSQDD1271 pKa = 3.09 AAFLNFEE1278 pKa = 4.32 LRR1280 pKa = 11.84 KK1281 pKa = 9.62 ARR1283 pKa = 11.84 HH1284 pKa = 5.61 FGVPEE1289 pKa = 3.95 EE1290 pKa = 4.32 VVDD1293 pKa = 5.57 FYY1295 pKa = 12.14 AFIKK1299 pKa = 8.86 THH1301 pKa = 6.42 AKK1303 pKa = 9.19 TFLGNLAVMRR1313 pKa = 11.84 LSGEE1317 pKa = 4.14 GPTFDD1322 pKa = 6.1 ANTEE1326 pKa = 4.05 CNIAYY1331 pKa = 8.34 DD1332 pKa = 3.78 ALRR1335 pKa = 11.84 FQLDD1339 pKa = 3.8 STVKK1343 pKa = 10.2 ACYY1346 pKa = 10.12 AGDD1349 pKa = 3.68 DD1350 pKa = 3.86 LVRR1353 pKa = 11.84 DD1354 pKa = 4.11 KK1355 pKa = 11.59 ACDD1358 pKa = 3.47 EE1359 pKa = 4.26 RR1360 pKa = 11.84 PGWKK1364 pKa = 9.91 YY1365 pKa = 11.05 SEE1367 pKa = 4.37 PLFSLKK1373 pKa = 10.54 AKK1375 pKa = 10.13 PLVTNKK1381 pKa = 9.9 PDD1383 pKa = 3.19 FCGWRR1388 pKa = 11.84 LTKK1391 pKa = 10.71 YY1392 pKa = 10.46 GIVKK1396 pKa = 10.52 SPVQLYY1402 pKa = 10.28 QSLQLALRR1410 pKa = 11.84 LGKK1413 pKa = 10.19 VEE1415 pKa = 4.03 EE1416 pKa = 4.42 VKK1418 pKa = 10.49 RR1419 pKa = 11.84 SYY1421 pKa = 11.23 AIDD1424 pKa = 3.38 YY1425 pKa = 9.93 LFAYY1429 pKa = 10.43 RR1430 pKa = 11.84 LGDD1433 pKa = 3.38 QLYY1436 pKa = 10.56 DD1437 pKa = 3.39 IFDD1440 pKa = 3.76 EE1441 pKa = 5.3 DD1442 pKa = 4.39 EE1443 pKa = 4.3 LSKK1446 pKa = 10.91 HH1447 pKa = 5.12 QLVTRR1452 pKa = 11.84 TLIKK1456 pKa = 10.51 KK1457 pKa = 9.59 GMQPPSSGEE1466 pKa = 3.8 HH1467 pKa = 6.19 LPTFHH1472 pKa = 6.1 VTSDD1476 pKa = 3.02 RR1477 pKa = 11.84 LIRR1480 pKa = 11.84 DD1481 pKa = 3.12 PRR1483 pKa = 11.84 AIKK1486 pKa = 10.66 VSTFEE1491 pKa = 3.76 SDD1493 pKa = 4.93 RR1494 pKa = 11.84 IILPLDD1500 pKa = 4.02 IIDD1503 pKa = 4.01 DD1504 pKa = 4.12 HH1505 pKa = 6.66 FSKK1508 pKa = 10.67 SGKK1511 pKa = 6.98 TDD1513 pKa = 3.25 RR1514 pKa = 11.84 AEE1516 pKa = 3.85 MTEE1519 pKa = 3.91 EE1520 pKa = 3.91 SNRR1523 pKa = 11.84 RR1524 pKa = 11.84 AVEE1527 pKa = 3.67 IGATTNVARR1536 pKa = 11.84 QDD1538 pKa = 3.49 PTLTDD1543 pKa = 4.15 LFPDD1547 pKa = 3.81 AFKK1550 pKa = 11.19
Molecular weight: 174.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.636
IPC2_protein 5.537
IPC_protein 5.601
Toseland 5.982
ProMoST 5.919
Dawson 5.817
Bjellqvist 5.855
Wikipedia 5.817
Rodwell 5.792
Grimsley 6.148
Solomon 5.817
Lehninger 5.804
Nozaki 6.071
DTASelect 6.262
Thurlkill 6.249
EMBOSS 6.211
Sillero 6.173
Patrickios 4.19
IPC_peptide 5.842
IPC2_peptide 6.173
IPC2.peptide.svr19 6.109
Protein with the highest isoelectric point:
>tr|O55604|O55604_9VIRU 40kDa protein OS=Garlic virus C OX=12431 PE=4 SV=1
MM1 pKa = 7.54 SFTPPPDD8 pKa = 3.43 YY9 pKa = 11.33 NKK11 pKa = 10.16 VYY13 pKa = 9.42 LTLAIGAAVGILVHH27 pKa = 5.69 TLRR30 pKa = 11.84 SNHH33 pKa = 5.54 LTHH36 pKa = 7.43 VGDD39 pKa = 4.01 NTHH42 pKa = 6.72 HH43 pKa = 6.66 LPHH46 pKa = 7.02 GGRR49 pKa = 11.84 YY50 pKa = 9.26 CDD52 pKa = 3.33 GNKK55 pKa = 9.7 RR56 pKa = 11.84 IHH58 pKa = 6.65 YY59 pKa = 7.95 NGPQAGSTHH68 pKa = 5.71 VSSVLPFFAAITLTLVIHH86 pKa = 6.94 FISCRR91 pKa = 11.84 RR92 pKa = 11.84 RR93 pKa = 11.84 RR94 pKa = 11.84 VCIRR98 pKa = 11.84 CSEE101 pKa = 4.07 PHH103 pKa = 5.82
Molecular weight: 11.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.922
IPC2_protein 9.033
IPC_protein 9.326
Toseland 9.209
ProMoST 9.297
Dawson 9.604
Bjellqvist 9.619
Wikipedia 9.838
Rodwell 9.633
Grimsley 9.663
Solomon 9.75
Lehninger 9.706
Nozaki 9.692
DTASelect 9.487
Thurlkill 9.487
EMBOSS 9.706
Sillero 9.692
Patrickios 4.38
IPC_peptide 9.736
IPC2_peptide 9.136
IPC2.peptide.svr19 7.803
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2651
103
1550
441.8
49.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.431 ± 0.629
1.773 ± 0.547
5.885 ± 0.851
5.583 ± 0.87
3.923 ± 0.52
4.904 ± 0.458
3.621 ± 0.739
4.866 ± 0.49
4.904 ± 0.912
10.788 ± 1.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.584 ± 0.205
4.64 ± 1.149
5.998 ± 0.209
5.017 ± 0.632
5.545 ± 0.364
6.828 ± 0.567
8.186 ± 0.718
4.828 ± 0.443
0.717 ± 0.182
2.98 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here