Listeria kieliensis
Average proteome isoelectric point is 6.37
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2426 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D8TJZ8|A0A3D8TJZ8_9LIST Aminoglycoside N(3)-acetyltransferase OS=Listeria kieliensis OX=1621700 GN=UR08_12530 PE=3 SV=1
MM1 pKa = 7.44 AKK3 pKa = 10.4 VMIVYY8 pKa = 10.7 ASMTGNTQEE17 pKa = 4.07 IADD20 pKa = 3.77 ILGEE24 pKa = 4.09 EE25 pKa = 4.34 LEE27 pKa = 4.55 KK28 pKa = 11.18 YY29 pKa = 10.33 DD30 pKa = 3.73 IEE32 pKa = 4.81 VEE34 pKa = 3.8 IEE36 pKa = 3.71 EE37 pKa = 5.03 CINVEE42 pKa = 4.0 PEE44 pKa = 3.69 EE45 pKa = 4.0 LLDD48 pKa = 4.11 YY49 pKa = 11.22 DD50 pKa = 3.8 GALIGGYY57 pKa = 8.69 TYY59 pKa = 11.29 DD60 pKa = 5.36 DD61 pKa = 3.82 GQLPDD66 pKa = 4.05 EE67 pKa = 4.84 FVDD70 pKa = 5.19 FYY72 pKa = 11.56 EE73 pKa = 5.81 DD74 pKa = 3.28 MADD77 pKa = 3.08 VDD79 pKa = 4.9 FSGKK83 pKa = 9.41 VCASFGSGDD92 pKa = 3.27 TFYY95 pKa = 11.39 DD96 pKa = 4.14 EE97 pKa = 4.93 FCLTVDD103 pKa = 4.72 LVEE106 pKa = 4.33 TRR108 pKa = 11.84 LKK110 pKa = 10.77 EE111 pKa = 3.88 KK112 pKa = 10.63 GATVPVEE119 pKa = 3.93 GLKK122 pKa = 10.37 VDD124 pKa = 5.0 LDD126 pKa = 3.87 PDD128 pKa = 3.69 EE129 pKa = 5.32 EE130 pKa = 4.28 DD131 pKa = 3.51 VEE133 pKa = 4.33 RR134 pKa = 11.84 AEE136 pKa = 5.24 AYY138 pKa = 9.07 AKK140 pKa = 10.49 KK141 pKa = 10.58 YY142 pKa = 11.06 VDD144 pKa = 3.4 VLLGNEE150 pKa = 3.97
Molecular weight: 16.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.734
IPC2_protein 3.656
IPC_protein 3.643
Toseland 3.427
ProMoST 3.77
Dawson 3.617
Bjellqvist 3.808
Wikipedia 3.528
Rodwell 3.465
Grimsley 3.338
Solomon 3.605
Lehninger 3.554
Nozaki 3.732
DTASelect 3.923
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.757
Patrickios 1.024
IPC_peptide 3.605
IPC2_peptide 3.732
IPC2.peptide.svr19 3.705
Protein with the highest isoelectric point:
>tr|A0A3D8TP97|A0A3D8TP97_9LIST MFS transporter OS=Listeria kieliensis OX=1621700 GN=UR08_06690 PE=4 SV=1
MM1 pKa = 7.87 PKK3 pKa = 9.55 MKK5 pKa = 8.8 THH7 pKa = 6.93 RR8 pKa = 11.84 GSAKK12 pKa = 9.28 RR13 pKa = 11.84 FKK15 pKa = 10.13 RR16 pKa = 11.84 TGSGKK21 pKa = 10.12 LKK23 pKa = 10.02 RR24 pKa = 11.84 RR25 pKa = 11.84 HH26 pKa = 5.89 AFVSHH31 pKa = 5.87 MFANKK36 pKa = 9.68 SKK38 pKa = 9.57 KK39 pKa = 8.58 QKK41 pKa = 10.18 RR42 pKa = 11.84 KK43 pKa = 8.88 LRR45 pKa = 11.84 KK46 pKa = 9.04 AAMVSAGDD54 pKa = 3.9 FKK56 pKa = 11.14 RR57 pKa = 11.84 IRR59 pKa = 11.84 QMVAKK64 pKa = 9.76 MKK66 pKa = 10.66
Molecular weight: 7.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 10.877
IPC_protein 12.384
Toseland 12.574
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.544
Grimsley 12.618
Solomon 13.056
Lehninger 12.954
Nozaki 12.574
DTASelect 12.559
Thurlkill 12.574
EMBOSS 13.071
Sillero 12.574
Patrickios 12.266
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.974
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2426
0
2426
732639
37
2189
302.0
33.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.782 ± 0.05
0.6 ± 0.013
5.149 ± 0.044
7.667 ± 0.058
4.873 ± 0.043
6.826 ± 0.041
1.846 ± 0.023
7.352 ± 0.049
7.134 ± 0.047
10.08 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.616 ± 0.022
4.096 ± 0.032
3.476 ± 0.027
3.63 ± 0.028
4.187 ± 0.038
5.916 ± 0.038
5.456 ± 0.04
7.042 ± 0.037
0.879 ± 0.016
3.394 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here