Sphingomonas sp. XS-10

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3831 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518RJI6|A0A518RJI6_9SPHN Ribosomal RNA large subunit methyltransferase H OS=Sphingomonas sp. XS-10 OX=2599297 GN=rlmH PE=3 SV=1
MM1 pKa = 7.32RR2 pKa = 11.84HH3 pKa = 5.35KK4 pKa = 9.65TLFSLIALCGSTAAVAAIGTPQTEE28 pKa = 4.14PPLQNDD34 pKa = 3.75IANEE38 pKa = 3.93ATPAPDD44 pKa = 5.36DD45 pKa = 3.54MTAPEE50 pKa = 4.27TMSNSTDD57 pKa = 3.36VEE59 pKa = 4.21PSMEE63 pKa = 4.25PAEE66 pKa = 4.38PADD69 pKa = 3.68PTEE72 pKa = 5.25DD73 pKa = 3.49GSEE76 pKa = 4.07TSPEE80 pKa = 3.85PNAAYY85 pKa = 10.34YY86 pKa = 10.49

Molecular weight: 8.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518RED7|A0A518RED7_9SPHN Uncharacterized protein OS=Sphingomonas sp. XS-10 OX=2599297 GN=FPZ54_07120 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84NKK41 pKa = 10.45LSAA44 pKa = 3.94

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3831

0

3831

1241582

29

3971

324.1

34.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.661 ± 0.062

0.718 ± 0.01

6.081 ± 0.03

5.348 ± 0.039

3.55 ± 0.026

8.949 ± 0.037

1.878 ± 0.021

4.968 ± 0.025

2.832 ± 0.032

9.754 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.02

2.458 ± 0.025

5.487 ± 0.036

3.012 ± 0.021

7.607 ± 0.046

5.063 ± 0.029

5.385 ± 0.032

7.178 ± 0.032

1.486 ± 0.015

2.228 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski