Erwinia phage vB_EamM_TropicalSun

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Myosmarvirus; unclassified Myosmarvirus

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NNZ4|A0A5B9NNZ4_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_TropicalSun OX=2591372 GN=TROPICALSUN_101 PE=4 SV=1
MM1 pKa = 7.16QFTVEE6 pKa = 4.62TINAAKK12 pKa = 9.95FADD15 pKa = 3.84AGCYY19 pKa = 7.31VTFEE23 pKa = 4.43SALASYY29 pKa = 10.17IGLKK33 pKa = 7.94ITRR36 pKa = 11.84EE37 pKa = 3.84LSEE40 pKa = 4.56EE41 pKa = 4.02EE42 pKa = 3.8FDD44 pKa = 4.2VISGEE49 pKa = 4.42FFDD52 pKa = 6.02DD53 pKa = 4.48DD54 pKa = 4.36EE55 pKa = 7.15RR56 pKa = 11.84DD57 pKa = 3.64TSSDD61 pKa = 2.93GSLEE65 pKa = 4.0IDD67 pKa = 3.02GVEE70 pKa = 3.93YY71 pKa = 11.15NFAIDD76 pKa = 4.88LGRR79 pKa = 11.84KK80 pKa = 8.97CLIIFYY86 pKa = 10.68AEE88 pKa = 3.99YY89 pKa = 11.1VKK91 pKa = 10.98DD92 pKa = 3.86EE93 pKa = 4.58NGNDD97 pKa = 3.53TDD99 pKa = 4.01EE100 pKa = 4.46VDD102 pKa = 3.76LFGAKK107 pKa = 10.26GFDD110 pKa = 3.21IEE112 pKa = 4.8EE113 pKa = 4.05II114 pKa = 3.88

Molecular weight: 12.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NI41|A0A5B9NI41_9CAUD Uncharacterized protein OS=Erwinia phage vB_EamM_TropicalSun OX=2591372 GN=TROPICALSUN_88 PE=4 SV=1
MM1 pKa = 7.32TKK3 pKa = 9.86IFKK6 pKa = 10.42CDD8 pKa = 3.47LNKK11 pKa = 10.26VIEE14 pKa = 4.25TARR17 pKa = 11.84LRR19 pKa = 11.84VVKK22 pKa = 9.75PRR24 pKa = 11.84RR25 pKa = 11.84ATAIVKK31 pKa = 9.91VNLVVGGQEE40 pKa = 4.15VAFHH44 pKa = 5.46CTARR48 pKa = 11.84RR49 pKa = 11.84TPGVARR55 pKa = 11.84VTFHH59 pKa = 7.02ANGRR63 pKa = 11.84AVDD66 pKa = 4.19LDD68 pKa = 3.5EE69 pKa = 4.78AAALFGSHH77 pKa = 6.66NSDD80 pKa = 2.54IDD82 pKa = 3.85VARR85 pKa = 11.84LAHH88 pKa = 6.52KK89 pKa = 9.93RR90 pKa = 11.84AKK92 pKa = 9.16QVEE95 pKa = 4.2YY96 pKa = 10.33NKK98 pKa = 10.29RR99 pKa = 11.84YY100 pKa = 9.6RR101 pKa = 11.84EE102 pKa = 4.21RR103 pKa = 11.84IRR105 pKa = 11.84SRR107 pKa = 11.84DD108 pKa = 3.04GSNNN112 pKa = 3.09

Molecular weight: 12.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

21537

37

959

205.1

22.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.933 ± 0.303

1.128 ± 0.093

6.143 ± 0.183

6.069 ± 0.322

4.183 ± 0.142

7.364 ± 0.236

1.695 ± 0.121

6.435 ± 0.19

6.319 ± 0.282

7.341 ± 0.198

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.13

4.838 ± 0.217

3.998 ± 0.168

3.863 ± 0.187

4.908 ± 0.202

6.496 ± 0.237

5.581 ± 0.263

6.918 ± 0.198

1.425 ± 0.112

3.427 ± 0.143
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski