Actinomadura sp. LD22

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8642 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6I4MKJ7|A0A6I4MKJ7_9ACTN Uncharacterized protein OS=Actinomadura sp. LD22 OX=2650748 GN=F8568_034720 PE=4 SV=1
MM1 pKa = 7.74RR2 pKa = 11.84VTVTDD7 pKa = 3.73TCQGCGQCEE16 pKa = 3.79AAVPDD21 pKa = 3.77VFRR24 pKa = 11.84IHH26 pKa = 7.77DD27 pKa = 4.39DD28 pKa = 3.57GFAVALVDD36 pKa = 4.61TIPEE40 pKa = 4.17PLEE43 pKa = 3.64QDD45 pKa = 3.3VRR47 pKa = 11.84DD48 pKa = 4.55AADD51 pKa = 3.18QCPTEE56 pKa = 5.06AIEE59 pKa = 4.21LTGG62 pKa = 3.78

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6I4MAL1|A0A6I4MAL1_9ACTN ATP-binding cassette domain-containing protein OS=Actinomadura sp. LD22 OX=2650748 GN=F8568_004670 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84HH14 pKa = 5.34KK15 pKa = 10.47KK16 pKa = 8.84HH17 pKa = 5.5GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLSSRR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.22GRR40 pKa = 11.84ARR42 pKa = 11.84VAVV45 pKa = 3.65

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8642

0

8642

2806568

21

4028

324.8

34.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.031 ± 0.041

0.799 ± 0.007

6.082 ± 0.02

5.606 ± 0.03

2.75 ± 0.016

9.763 ± 0.029

2.206 ± 0.014

3.382 ± 0.018

2.048 ± 0.019

10.137 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.01

1.692 ± 0.013

6.342 ± 0.025

2.512 ± 0.017

8.656 ± 0.03

4.71 ± 0.02

5.523 ± 0.021

8.41 ± 0.024

1.479 ± 0.012

2.008 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski