Amino acid dipepetide frequency for Actinomadura sp. LD22

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	23.393AlaAla: 23.393 ± 0.175	1.159AlaCys: 1.159 ± 0.022	8.386AlaAsp: 8.386 ± 0.057	8.979AlaGlu: 8.979 ± 0.092	3.881AlaPhe: 3.881 ± 0.038	14.563AlaGly: 14.563 ± 0.086	2.717AlaHis: 2.717 ± 0.035	4.078AlaIle: 4.078 ± 0.038	2.803AlaLys: 2.803 ± 0.039	14.381AlaLeu: 14.381 ± 0.098	2.685AlaMet: 2.685 ± 0.03	1.971AlaAsn: 1.971 ± 0.026	7.446AlaPro: 7.446 ± 0.062	3.439AlaGln: 3.439 ± 0.039	11.526AlaArg: 11.526 ± 0.086	6.057AlaSer: 6.057 ± 0.057	6.238AlaThr: 6.238 ± 0.052	12.117AlaVal: 12.117 ± 0.09	1.889AlaTrp: 1.889 ± 0.023	2.599AlaTyr: 2.599 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	1.081CysAla: 1.081 ± 0.021	0.105CysCys: 0.105 ± 0.007	0.54CysAsp: 0.54 ± 0.018	0.417CysGlu: 0.417 ± 0.014	0.228CysPhe: 0.228 ± 0.009	1.025CysGly: 1.025 ± 0.02	0.196CysHis: 0.196 ± 0.009	0.161CysIle: 0.161 ± 0.007	0.124CysLys: 0.124 ± 0.006	0.71CysLeu: 0.71 ± 0.017	0.135CysMet: 0.135 ± 0.007	0.131CysAsn: 0.131 ± 0.007	0.511CysPro: 0.511 ± 0.016	0.169CysGln: 0.169 ± 0.008	0.656CysArg: 0.656 ± 0.017	0.408CysSer: 0.408 ± 0.013	0.446CysThr: 0.446 ± 0.013	0.663CysVal: 0.663 ± 0.016	0.114CysTrp: 0.114 ± 0.007	0.168CysTyr: 0.168 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	8.284AspAla: 8.284 ± 0.064	0.394AspCys: 0.394 ± 0.013	3.864AspAsp: 3.864 ± 0.043	4.055AspGlu: 4.055 ± 0.041	1.52AspPhe: 1.52 ± 0.025	6.681AspGly: 6.681 ± 0.059	1.378AspHis: 1.378 ± 0.022	1.791AspIle: 1.791 ± 0.026	1.073AspLys: 1.073 ± 0.023	6.753AspLeu: 6.753 ± 0.049	0.899AspMet: 0.899 ± 0.019	0.785AspAsn: 0.785 ± 0.019	4.792AspPro: 4.792 ± 0.046	1.495AspGln: 1.495 ± 0.024	5.365AspArg: 5.365 ± 0.046	1.966AspSer: 1.966 ± 0.028	2.535AspThr: 2.535 ± 0.032	5.16AspVal: 5.16 ± 0.039	0.929AspTrp: 0.929 ± 0.017	1.102AspTyr: 1.102 ± 0.021	0.0AspXaa: 0.0 ± 0.0
Glu	7.303GluAla: 7.303 ± 0.072	0.355GluCys: 0.355 ± 0.012	2.808GluAsp: 2.808 ± 0.035	3.229GluGlu: 3.229 ± 0.046	1.485GluPhe: 1.485 ± 0.026	3.966GluGly: 3.966 ± 0.041	1.655GluHis: 1.655 ± 0.024	2.499GluIle: 2.499 ± 0.029	1.225GluLys: 1.225 ± 0.022	6.456GluLeu: 6.456 ± 0.057	1.051GluMet: 1.051 ± 0.023	0.928GluAsn: 0.928 ± 0.019	3.698GluPro: 3.698 ± 0.043	1.973GluGln: 1.973 ± 0.029	6.0GluArg: 6.0 ± 0.061	2.308GluSer: 2.308 ± 0.033	2.77GluThr: 2.77 ± 0.04	4.494GluVal: 4.494 ± 0.042	0.779GluTrp: 0.779 ± 0.016	1.079GluTyr: 1.079 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	3.866PheAla: 3.866 ± 0.041	0.3PheCys: 0.3 ± 0.01	1.993PheAsp: 1.993 ± 0.025	1.459PheGlu: 1.459 ± 0.027	0.843PhePhe: 0.843 ± 0.019	3.184PheGly: 3.184 ± 0.042	0.61PheHis: 0.61 ± 0.016	0.742PheIle: 0.742 ± 0.016	0.492PheLys: 0.492 ± 0.015	2.523PheLeu: 2.523 ± 0.028	0.436PheMet: 0.436 ± 0.012	0.541PheAsn: 0.541 ± 0.017	1.435PhePro: 1.435 ± 0.023	0.679PheGln: 0.679 ± 0.016	1.894PheArg: 1.894 ± 0.026	1.357PheSer: 1.357 ± 0.02	1.898PheThr: 1.898 ± 0.031	2.238PheVal: 2.238 ± 0.03	0.43PheTrp: 0.43 ± 0.014	0.582PheTyr: 0.582 ± 0.016	0.0PheXaa: 0.0 ± 0.0
Gly	11.845GlyAla: 11.845 ± 0.077	0.852GlyCys: 0.852 ± 0.019	5.575GlyAsp: 5.575 ± 0.049	5.293GlyGlu: 5.293 ± 0.056	2.917GlyPhe: 2.917 ± 0.034	9.905GlyGly: 9.905 ± 0.096	2.296GlyHis: 2.296 ± 0.028	3.401GlyIle: 3.401 ± 0.035	2.402GlyLys: 2.402 ± 0.039	9.688GlyLeu: 9.688 ± 0.072	2.104GlyMet: 2.104 ± 0.029	1.642GlyAsn: 1.642 ± 0.027	5.654GlyPro: 5.654 ± 0.049	2.405GlyGln: 2.405 ± 0.035	8.93GlyArg: 8.93 ± 0.06	4.817GlySer: 4.817 ± 0.051	6.102GlyThr: 6.102 ± 0.055	7.747GlyVal: 7.747 ± 0.058	1.73GlyTrp: 1.73 ± 0.026	2.328GlyTyr: 2.328 ± 0.03	0.0GlyXaa: 0.0 ± 0.0
His	2.725HisAla: 2.725 ± 0.035	0.211HisCys: 0.211 ± 0.009	1.356HisAsp: 1.356 ± 0.025	1.149HisGlu: 1.149 ± 0.02	0.584HisPhe: 0.584 ± 0.013	2.448HisGly: 2.448 ± 0.032	0.655HisHis: 0.655 ± 0.017	0.69HisIle: 0.69 ± 0.017	0.336HisLys: 0.336 ± 0.011	2.38HisLeu: 2.38 ± 0.032	0.355HisMet: 0.355 ± 0.013	0.351HisAsn: 0.351 ± 0.011	1.715HisPro: 1.715 ± 0.026	0.548HisGln: 0.548 ± 0.015	2.097HisArg: 2.097 ± 0.028	0.852HisSer: 0.852 ± 0.019	1.039HisThr: 1.039 ± 0.019	1.741HisVal: 1.741 ± 0.024	0.348HisTrp: 0.348 ± 0.011	0.48HisTyr: 0.48 ± 0.012	0.0HisXaa: 0.0 ± 0.0
Ile	5.299IleAla: 5.299 ± 0.046	0.32IleCys: 0.32 ± 0.012	2.411IleAsp: 2.411 ± 0.029	2.181IleGlu: 2.181 ± 0.029	0.755IlePhe: 0.755 ± 0.017	3.845IleGly: 3.845 ± 0.037	0.557IleHis: 0.557 ± 0.016	1.043IleIle: 1.043 ± 0.021	0.692IleLys: 0.692 ± 0.016	2.466IleLeu: 2.466 ± 0.031	0.547IleMet: 0.547 ± 0.012	0.715IleAsn: 0.715 ± 0.018	1.823IlePro: 1.823 ± 0.026	0.693IleGln: 0.693 ± 0.015	2.379IleArg: 2.379 ± 0.029	1.659IleSer: 1.659 ± 0.024	2.227IleThr: 2.227 ± 0.031	3.236IleVal: 3.236 ± 0.039	0.405IleTrp: 0.405 ± 0.012	0.566IleTyr: 0.566 ± 0.017	0.0IleXaa: 0.0 ± 0.0
Lys	2.868LysAla: 2.868 ± 0.041	0.107LysCys: 0.107 ± 0.006	1.21LysAsp: 1.21 ± 0.023	1.068LysGlu: 1.068 ± 0.023	0.422LysPhe: 0.422 ± 0.013	1.743LysGly: 1.743 ± 0.023	0.428LysHis: 0.428 ± 0.013	0.961LysIle: 0.961 ± 0.022	0.692LysLys: 0.692 ± 0.021	1.835LysLeu: 1.835 ± 0.032	0.409LysMet: 0.409 ± 0.012	0.443LysAsn: 0.443 ± 0.014	1.332LysPro: 1.332 ± 0.024	0.629LysGln: 0.629 ± 0.02	1.544LysArg: 1.544 ± 0.023	1.045LysSer: 1.045 ± 0.023	1.191LysThr: 1.191 ± 0.022	1.878LysVal: 1.878 ± 0.028	0.256LysTrp: 0.256 ± 0.008	0.423LysTyr: 0.423 ± 0.013	0.0LysXaa: 0.0 ± 0.0
Leu	15.554LeuAla: 15.554 ± 0.104	0.829LeuCys: 0.829 ± 0.015	6.968LeuAsp: 6.968 ± 0.056	4.916LeuGlu: 4.916 ± 0.053	2.58LeuPhe: 2.58 ± 0.032	9.286LeuGly: 9.286 ± 0.075	2.138LeuHis: 2.138 ± 0.029	3.336LeuIle: 3.336 ± 0.038	1.94LeuLys: 1.94 ± 0.028	10.515LeuLeu: 10.515 ± 0.084	1.611LeuMet: 1.611 ± 0.022	1.737LeuAsn: 1.737 ± 0.024	6.389LeuPro: 6.389 ± 0.047	2.084LeuGln: 2.084 ± 0.029	8.916LeuArg: 8.916 ± 0.071	4.918LeuSer: 4.918 ± 0.042	6.076LeuThr: 6.076 ± 0.046	8.557LeuVal: 8.557 ± 0.065	1.269LeuTrp: 1.269 ± 0.021	1.757LeuTyr: 1.757 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.507MetAla: 2.507 ± 0.032	0.149MetCys: 0.149 ± 0.007	0.984MetAsp: 0.984 ± 0.018	0.768MetGlu: 0.768 ± 0.017	0.503MetPhe: 0.503 ± 0.014	1.405MetGly: 1.405 ± 0.023	0.377MetHis: 0.377 ± 0.012	0.779MetIle: 0.779 ± 0.017	0.428MetLys: 0.428 ± 0.013	1.881MetLeu: 1.881 ± 0.028	0.338MetMet: 0.338 ± 0.012	0.437MetAsn: 0.437 ± 0.013	1.223MetPro: 1.223 ± 0.023	0.449MetGln: 0.449 ± 0.012	1.722MetArg: 1.722 ± 0.025	1.252MetSer: 1.252 ± 0.021	1.539MetThr: 1.539 ± 0.025	1.356MetVal: 1.356 ± 0.023	0.229MetTrp: 0.229 ± 0.009	0.306MetTyr: 0.306 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.258AsnAla: 2.258 ± 0.03	0.161AsnCys: 0.161 ± 0.008	0.945AsnAsp: 0.945 ± 0.019	0.811AsnGlu: 0.811 ± 0.02	0.435AsnPhe: 0.435 ± 0.012	1.883AsnGly: 1.883 ± 0.03	0.348AsnHis: 0.348 ± 0.009	0.61AsnIle: 0.61 ± 0.015	0.365AsnLys: 0.365 ± 0.012	1.659AsnLeu: 1.659 ± 0.027	0.289AsnMet: 0.289 ± 0.011	0.325AsnAsn: 0.325 ± 0.012	1.326AsnPro: 1.326 ± 0.024	0.464AsnGln: 0.464 ± 0.013	1.274AsnArg: 1.274 ± 0.023	0.725AsnSer: 0.725 ± 0.017	0.958AsnThr: 0.958 ± 0.02	1.446AsnVal: 1.446 ± 0.024	0.245AsnTrp: 0.245 ± 0.009	0.389AsnTyr: 0.389 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	9.237ProAla: 9.237 ± 0.079	0.351ProCys: 0.351 ± 0.01	4.91ProAsp: 4.91 ± 0.045	4.148ProGlu: 4.148 ± 0.043	1.662ProPhe: 1.662 ± 0.024	7.333ProGly: 7.333 ± 0.067	1.35ProHis: 1.35 ± 0.023	1.586ProIle: 1.586 ± 0.022	1.254ProLys: 1.254 ± 0.023	5.232ProLeu: 5.232 ± 0.046	1.118ProMet: 1.118 ± 0.022	0.953ProAsn: 0.953 ± 0.021	4.499ProPro: 4.499 ± 0.071	1.605ProGln: 1.605 ± 0.026	4.371ProArg: 4.371 ± 0.035	3.465ProSer: 3.465 ± 0.046	2.734ProThr: 2.734 ± 0.038	5.372ProVal: 5.372 ± 0.05	0.908ProTrp: 0.908 ± 0.02	1.335ProTyr: 1.335 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.503GlnAla: 3.503 ± 0.045	0.176GlnCys: 0.176 ± 0.008	1.303GlnAsp: 1.303 ± 0.024	1.289GlnGlu: 1.289 ± 0.021	0.639GlnPhe: 0.639 ± 0.016	2.154GlnGly: 2.154 ± 0.031	0.556GlnHis: 0.556 ± 0.015	1.161GlnIle: 1.161 ± 0.02	0.562GlnLys: 0.562 ± 0.018	2.416GlnLeu: 2.416 ± 0.03	0.522GlnMet: 0.522 ± 0.014	0.47GlnAsn: 0.47 ± 0.014	1.486GlnPro: 1.486 ± 0.029	0.989GlnGln: 0.989 ± 0.03	2.325GlnArg: 2.325 ± 0.032	1.067GlnSer: 1.067 ± 0.02	1.239GlnThr: 1.239 ± 0.021	2.343GlnVal: 2.343 ± 0.029	0.411GlnTrp: 0.411 ± 0.013	0.507GlnTyr: 0.507 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	10.954ArgAla: 10.954 ± 0.083	0.642ArgCys: 0.642 ± 0.017	5.082ArgAsp: 5.082 ± 0.053	4.881ArgGlu: 4.881 ± 0.047	2.523ArgPhe: 2.523 ± 0.028	6.332ArgGly: 6.332 ± 0.048	2.18ArgHis: 2.18 ± 0.027	3.408ArgIle: 3.408 ± 0.037	1.616ArgLys: 1.616 ± 0.024	9.636ArgLeu: 9.636 ± 0.064	1.972ArgMet: 1.972 ± 0.028	1.369ArgAsn: 1.369 ± 0.023	5.864ArgPro: 5.864 ± 0.055	2.118ArgGln: 2.118 ± 0.03	9.095ArgArg: 9.095 ± 0.069	4.035ArgSer: 4.035 ± 0.04	5.104ArgThr: 5.104 ± 0.044	6.374ArgVal: 6.374 ± 0.051	1.44ArgTrp: 1.44 ± 0.024	1.94ArgTyr: 1.94 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	6.279SerAla: 6.279 ± 0.052	0.379SerCys: 0.379 ± 0.014	2.498SerAsp: 2.498 ± 0.032	2.149SerGlu: 2.149 ± 0.029	1.417SerPhe: 1.417 ± 0.023	5.908SerGly: 5.908 ± 0.052	0.863SerHis: 0.863 ± 0.021	1.549SerIle: 1.549 ± 0.026	0.983SerLys: 0.983 ± 0.018	4.386SerLeu: 4.386 ± 0.043	1.107SerMet: 1.107 ± 0.021	0.759SerAsn: 0.759 ± 0.019	3.164SerPro: 3.164 ± 0.038	1.074SerGln: 1.074 ± 0.02	3.681SerArg: 3.681 ± 0.042	2.577SerSer: 2.577 ± 0.04	2.587SerThr: 2.587 ± 0.036	3.819SerVal: 3.819 ± 0.035	0.823SerTrp: 0.823 ± 0.017	1.098SerTyr: 1.098 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.047ThrAla: 8.047 ± 0.06	0.428ThrCys: 0.428 ± 0.012	3.06ThrAsp: 3.06 ± 0.035	2.771ThrGlu: 2.771 ± 0.036	1.567ThrPhe: 1.567 ± 0.026	6.52ThrGly: 6.52 ± 0.06	0.997ThrHis: 0.997 ± 0.02	1.887ThrIle: 1.887 ± 0.027	1.037ThrLys: 1.037 ± 0.024	5.211ThrLeu: 5.211 ± 0.043	0.997ThrMet: 0.997 ± 0.017	0.869ThrAsn: 0.869 ± 0.021	3.566ThrPro: 3.566 ± 0.039	1.108ThrGln: 1.108 ± 0.022	3.908ThrArg: 3.908 ± 0.035	2.808ThrSer: 2.808 ± 0.037	3.122ThrThr: 3.122 ± 0.042	5.323ThrVal: 5.323 ± 0.055	0.867ThrTrp: 0.867 ± 0.02	1.138ThrTyr: 1.138 ± 0.029	0.0ThrXaa: 0.0 ± 0.0
Val	11.08ValAla: 11.08 ± 0.069	0.731ValCys: 0.731 ± 0.017	4.722ValAsp: 4.722 ± 0.042	4.623ValGlu: 4.623 ± 0.046	2.428ValPhe: 2.428 ± 0.034	6.196ValGly: 6.196 ± 0.062	1.972ValHis: 1.972 ± 0.025	3.044ValIle: 3.044 ± 0.034	1.697ValLys: 1.697 ± 0.03	9.462ValLeu: 9.462 ± 0.076	1.473ValMet: 1.473 ± 0.024	1.721ValAsn: 1.721 ± 0.029	5.613ValPro: 5.613 ± 0.049	2.132ValGln: 2.132 ± 0.027	7.593ValArg: 7.593 ± 0.056	4.052ValSer: 4.052 ± 0.045	5.235ValThr: 5.235 ± 0.049	7.754ValVal: 7.754 ± 0.062	1.063ValTrp: 1.063 ± 0.018	1.512ValTyr: 1.512 ± 0.026	0.0ValXaa: 0.0 ± 0.0
Trp	1.638TrpAla: 1.638 ± 0.024	0.154TrpCys: 0.154 ± 0.008	0.854TrpAsp: 0.854 ± 0.019	0.711TrpGlu: 0.711 ± 0.016	0.5TrpPhe: 0.5 ± 0.012	1.037TrpGly: 1.037 ± 0.02	0.381TrpHis: 0.381 ± 0.012	0.564TrpIle: 0.564 ± 0.017	0.352TrpLys: 0.352 ± 0.012	1.688TrpLeu: 1.688 ± 0.024	0.287TrpMet: 0.287 ± 0.01	0.382TrpAsn: 0.382 ± 0.011	0.841TrpPro: 0.841 ± 0.017	0.488TrpGln: 0.488 ± 0.015	1.46TrpArg: 1.46 ± 0.024	0.821TrpSer: 0.821 ± 0.017	1.084TrpThr: 1.084 ± 0.023	0.888TrpVal: 0.888 ± 0.02	0.326TrpTrp: 0.326 ± 0.012	0.333TrpTyr: 0.333 ± 0.01	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.587TyrAla: 2.587 ± 0.03	0.185TyrCys: 0.185 ± 0.008	1.351TyrAsp: 1.351 ± 0.03	1.162TyrGlu: 1.162 ± 0.022	0.61TyrPhe: 0.61 ± 0.015	2.22TyrGly: 2.22 ± 0.03	0.406TyrHis: 0.406 ± 0.012	0.53TyrIle: 0.53 ± 0.014	0.41TyrLys: 0.41 ± 0.012	2.097TyrLeu: 2.097 ± 0.031	0.295TyrMet: 0.295 ± 0.011	0.384TyrAsn: 0.384 ± 0.012	1.049TyrPro: 1.049 ± 0.02	0.578TyrGln: 0.578 ± 0.016	1.829TyrArg: 1.829 ± 0.024	0.913TyrSer: 0.913 ± 0.021	1.108TyrThr: 1.108 ± 0.026	1.597TyrVal: 1.597 ± 0.026	0.33TyrTrp: 0.33 ± 0.011	0.435TyrTyr: 0.435 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 8642 proteins (2806568 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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