Flavobacterium croceum DSM 17960
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2701 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S4N9F2|A0A2S4N9F2_9FLAO Arabinose-5-phosphate isomerase OS=Flavobacterium croceum DSM 17960 OX=1121886 GN=Q361_10424 PE=3 SV=1
MM1 pKa = 7.57 KK2 pKa = 10.43 KK3 pKa = 10.44 LYY5 pKa = 10.47 LQILLFFIGLATTVAQVPNYY25 pKa = 9.31 NVVSIPYY32 pKa = 9.1 QNVVLSPTMASTTISFNLDD51 pKa = 3.14 DD52 pKa = 4.76 TYY54 pKa = 11.39 SGPIALPFNINFYY67 pKa = 11.05 GVTNNFMNVSSNGYY81 pKa = 7.95 ITFNSNAAGSNSPWSFNTTIPNAGFPVKK109 pKa = 10.41 NAFLGCYY116 pKa = 9.53 HH117 pKa = 7.79 DD118 pKa = 4.3 INNAIVNTTDD128 pKa = 3.38 PQSVVYY134 pKa = 10.12 SIQGYY139 pKa = 9.47 APFRR143 pKa = 11.84 KK144 pKa = 10.18 VIVLYY149 pKa = 10.99 SNNPQFSCNNSAISTFAMIMYY170 pKa = 10.14 EE171 pKa = 4.17 DD172 pKa = 4.35 GEE174 pKa = 4.39 KK175 pKa = 10.44 FDD177 pKa = 3.92 VQIKK181 pKa = 10.56 RR182 pKa = 11.84 KK183 pKa = 9.65 DD184 pKa = 3.41 LCATWNGGRR193 pKa = 11.84 AVVGLIDD200 pKa = 3.71 PTGASAIAAPGRR212 pKa = 11.84 NTSAWTANYY221 pKa = 7.53 EE222 pKa = 3.53 AWRR225 pKa = 11.84 FYY227 pKa = 10.6 NTFGANHH234 pKa = 5.8 YY235 pKa = 9.9 KK236 pKa = 10.12 FWKK239 pKa = 10.38 CEE241 pKa = 3.7 TDD243 pKa = 3.48 GDD245 pKa = 4.1 GLEE248 pKa = 4.35 PFNLAVVKK256 pKa = 10.06 TDD258 pKa = 3.61 LNEE261 pKa = 4.11 PSMTFYY267 pKa = 11.04 SGIINGQVDD276 pKa = 4.42 VNTALPEE283 pKa = 4.63 LYY285 pKa = 10.65 QNTTAFNQKK294 pKa = 9.62 IYY296 pKa = 10.48 GLKK299 pKa = 8.58 STGEE303 pKa = 3.92 IVTIDD308 pKa = 3.94 LKK310 pKa = 11.47 VFSCDD315 pKa = 3.53 NDD317 pKa = 3.37 YY318 pKa = 11.79 DD319 pKa = 4.54 LDD321 pKa = 3.99 NVTTSLEE328 pKa = 4.02 DD329 pKa = 3.86 LNNDD333 pKa = 3.34 TNLANDD339 pKa = 4.01 DD340 pKa = 3.77 TDD342 pKa = 5.62 ADD344 pKa = 4.0 GTPNFIDD351 pKa = 4.09 NDD353 pKa = 4.04 DD354 pKa = 4.81 DD355 pKa = 5.72 GDD357 pKa = 4.9 LILTSIEE364 pKa = 4.06 YY365 pKa = 10.55 VFNSNKK371 pKa = 8.06 NTNATSTTNSVLDD384 pKa = 3.98 TDD386 pKa = 4.19 NDD388 pKa = 4.87 GIPNYY393 pKa = 10.35 LDD395 pKa = 4.14 NDD397 pKa = 4.19 DD398 pKa = 5.75 DD399 pKa = 5.99 GDD401 pKa = 4.24 GVLTMNEE408 pKa = 4.31 DD409 pKa = 3.79 YY410 pKa = 11.21 NGNRR414 pKa = 11.84 NPADD418 pKa = 4.5 DD419 pKa = 4.24 DD420 pKa = 4.38 TNGNSIPDD428 pKa = 3.7 YY429 pKa = 11.02 LEE431 pKa = 3.84 TSVALGTAQNILDD444 pKa = 4.26 SYY446 pKa = 9.05 FTVYY450 pKa = 10.42 PNPANDD456 pKa = 3.42 VLFIKK461 pKa = 10.72 NNNFNNADD469 pKa = 3.33 IKK471 pKa = 11.09 VKK473 pKa = 10.14 IVSVTGAVVKK483 pKa = 10.52 EE484 pKa = 3.92 MSTQNEE490 pKa = 3.96 ITPISVSALQSGMYY504 pKa = 9.2 IVEE507 pKa = 5.08 AISSEE512 pKa = 3.74 GTSYY516 pKa = 11.29 HH517 pKa = 6.81 KK518 pKa = 10.45 FIKK521 pKa = 10.27 KK522 pKa = 9.85
Molecular weight: 57.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.781
IPC2_protein 3.973
IPC_protein 4.012
Toseland 3.783
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.948
Rodwell 3.834
Grimsley 3.681
Solomon 3.999
Lehninger 3.961
Nozaki 4.113
DTASelect 4.406
Thurlkill 3.834
EMBOSS 3.961
Sillero 4.126
Patrickios 1.011
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 3.998
Protein with the highest isoelectric point:
>tr|A0A2S4N809|A0A2S4N809_9FLAO Phosphoribosyl pyrophosphate synthase OS=Flavobacterium croceum DSM 17960 OX=1121886 GN=Q361_10721 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.53 HH17 pKa = 4.24 GFMEE21 pKa = 5.09 RR22 pKa = 11.84 MATANGRR29 pKa = 11.84 KK30 pKa = 8.93 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.21 GRR40 pKa = 11.84 HH41 pKa = 5.23 KK42 pKa = 10.67 LSVSSEE48 pKa = 3.91 PRR50 pKa = 11.84 HH51 pKa = 5.92 KK52 pKa = 10.61 KK53 pKa = 9.84
Molecular weight: 6.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 10.716
IPC_protein 12.266
Toseland 12.442
ProMoST 12.925
Dawson 12.442
Bjellqvist 12.427
Wikipedia 12.91
Rodwell 12.252
Grimsley 12.486
Solomon 12.939
Lehninger 12.837
Nozaki 12.442
DTASelect 12.427
Thurlkill 12.442
EMBOSS 12.939
Sillero 12.442
Patrickios 11.974
IPC_peptide 12.939
IPC2_peptide 11.915
IPC2.peptide.svr19 9.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2701
0
2701
889783
28
2406
329.4
37.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.067 ± 0.054
0.86 ± 0.015
4.966 ± 0.038
6.247 ± 0.057
5.359 ± 0.038
5.822 ± 0.045
1.753 ± 0.024
8.215 ± 0.048
8.383 ± 0.065
9.079 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.115 ± 0.027
6.841 ± 0.062
3.281 ± 0.026
3.773 ± 0.031
2.859 ± 0.036
6.469 ± 0.048
6.34 ± 0.076
6.151 ± 0.038
0.984 ± 0.015
4.434 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here