Amino acid dipepetide frequency for Flavobacterium croceum DSM 17960

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.373AlaAla: 3.373 ± 0.097	0.588AlaCys: 0.588 ± 0.036	2.733AlaAsp: 2.733 ± 0.057	3.619AlaGlu: 3.619 ± 0.074	3.011AlaPhe: 3.011 ± 0.057	3.74AlaGly: 3.74 ± 0.094	1.065AlaHis: 1.065 ± 0.033	5.086AlaIle: 5.086 ± 0.081	4.92AlaLys: 4.92 ± 0.084	5.932AlaLeu: 5.932 ± 0.089	1.353AlaMet: 1.353 ± 0.045	3.602AlaAsn: 3.602 ± 0.08	1.853AlaPro: 1.853 ± 0.063	2.764AlaGln: 2.764 ± 0.062	1.632AlaArg: 1.632 ± 0.047	4.206AlaSer: 4.206 ± 0.096	4.412AlaThr: 4.412 ± 0.12	3.89AlaVal: 3.89 ± 0.085	0.515AlaTrp: 0.515 ± 0.023	2.374AlaTyr: 2.374 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.53CysAla: 0.53 ± 0.028	0.103CysCys: 0.103 ± 0.012	0.462CysAsp: 0.462 ± 0.025	0.468CysGlu: 0.468 ± 0.025	0.447CysPhe: 0.447 ± 0.025	0.68CysGly: 0.68 ± 0.029	0.184CysHis: 0.184 ± 0.016	0.688CysIle: 0.688 ± 0.03	0.597CysLys: 0.597 ± 0.028	0.677CysLeu: 0.677 ± 0.028	0.166CysMet: 0.166 ± 0.015	0.572CysAsn: 0.572 ± 0.026	0.391CysPro: 0.391 ± 0.029	0.251CysGln: 0.251 ± 0.018	0.208CysArg: 0.208 ± 0.016	0.671CysSer: 0.671 ± 0.036	0.538CysThr: 0.538 ± 0.027	0.508CysVal: 0.508 ± 0.029	0.061CysTrp: 0.061 ± 0.008	0.402CysTyr: 0.402 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	3.366AspAla: 3.366 ± 0.07	0.434AspCys: 0.434 ± 0.023	2.321AspAsp: 2.321 ± 0.064	3.273AspGlu: 3.273 ± 0.076	3.517AspPhe: 3.517 ± 0.072	2.932AspGly: 2.932 ± 0.076	0.637AspHis: 0.637 ± 0.027	4.159AspIle: 4.159 ± 0.073	4.413AspLys: 4.413 ± 0.072	4.508AspLeu: 4.508 ± 0.081	0.982AspMet: 0.982 ± 0.041	3.102AspAsn: 3.102 ± 0.066	1.205AspPro: 1.205 ± 0.042	1.09AspGln: 1.09 ± 0.036	1.485AspArg: 1.485 ± 0.04	2.707AspSer: 2.707 ± 0.056	2.946AspThr: 2.946 ± 0.053	3.203AspVal: 3.203 ± 0.061	0.62AspTrp: 0.62 ± 0.028	2.76AspTyr: 2.76 ± 0.062	0.0AspXaa: 0.0 ± 0.0
Glu	3.716GluAla: 3.716 ± 0.072	0.361GluCys: 0.361 ± 0.019	2.882GluAsp: 2.882 ± 0.07	4.145GluGlu: 4.145 ± 0.094	2.862GluPhe: 2.862 ± 0.07	3.039GluGly: 3.039 ± 0.069	1.133GluHis: 1.133 ± 0.039	5.731GluIle: 5.731 ± 0.089	6.535GluLys: 6.535 ± 0.103	5.742GluLeu: 5.742 ± 0.096	1.532GluMet: 1.532 ± 0.04	5.269GluAsn: 5.269 ± 0.093	1.49GluPro: 1.49 ± 0.042	2.49GluGln: 2.49 ± 0.068	2.041GluArg: 2.041 ± 0.061	2.896GluSer: 2.896 ± 0.062	3.574GluThr: 3.574 ± 0.068	3.91GluVal: 3.91 ± 0.068	0.519GluTrp: 0.519 ± 0.022	2.607GluTyr: 2.607 ± 0.055	0.0GluXaa: 0.0 ± 0.0
Phe	3.007PheAla: 3.007 ± 0.062	0.529PheCys: 0.529 ± 0.024	3.22PheAsp: 3.22 ± 0.064	3.568PheGlu: 3.568 ± 0.08	2.932PhePhe: 2.932 ± 0.076	3.514PheGly: 3.514 ± 0.065	0.845PheHis: 0.845 ± 0.032	4.179PheIle: 4.179 ± 0.092	3.796PheLys: 3.796 ± 0.069	4.799PheLeu: 4.799 ± 0.08	1.108PheMet: 1.108 ± 0.039	3.304PheAsn: 3.304 ± 0.076	1.661PhePro: 1.661 ± 0.045	1.725PheGln: 1.725 ± 0.049	1.376PheArg: 1.376 ± 0.039	4.282PheSer: 4.282 ± 0.078	3.419PheThr: 3.419 ± 0.068	3.348PheVal: 3.348 ± 0.076	0.573PheTrp: 0.573 ± 0.028	2.399PheTyr: 2.399 ± 0.059	0.0PheXaa: 0.0 ± 0.0
Gly	3.678GlyAla: 3.678 ± 0.087	0.609GlyCys: 0.609 ± 0.026	2.682GlyAsp: 2.682 ± 0.064	2.966GlyGlu: 2.966 ± 0.067	3.43GlyPhe: 3.43 ± 0.07	3.989GlyGly: 3.989 ± 0.118	0.98GlyHis: 0.98 ± 0.032	5.069GlyIle: 5.069 ± 0.091	4.983GlyLys: 4.983 ± 0.089	5.019GlyLeu: 5.019 ± 0.078	1.435GlyMet: 1.435 ± 0.041	3.607GlyAsn: 3.607 ± 0.081	1.052GlyPro: 1.052 ± 0.035	1.686GlyGln: 1.686 ± 0.051	1.612GlyArg: 1.612 ± 0.051	3.695GlySer: 3.695 ± 0.093	4.331GlyThr: 4.331 ± 0.144	3.965GlyVal: 3.965 ± 0.074	0.687GlyTrp: 0.687 ± 0.031	2.749GlyTyr: 2.749 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	0.917HisAla: 0.917 ± 0.034	0.193HisCys: 0.193 ± 0.017	0.816HisAsp: 0.816 ± 0.032	0.996HisGlu: 0.996 ± 0.028	1.23HisPhe: 1.23 ± 0.04	0.906HisGly: 0.906 ± 0.031	0.492HisHis: 0.492 ± 0.024	1.457HisIle: 1.457 ± 0.045	1.28HisLys: 1.28 ± 0.038	1.744HisLeu: 1.744 ± 0.05	0.264HisMet: 0.264 ± 0.018	1.091HisAsn: 1.091 ± 0.034	0.704HisPro: 0.704 ± 0.031	0.775HisGln: 0.775 ± 0.033	0.566HisArg: 0.566 ± 0.026	1.017HisSer: 1.017 ± 0.036	1.046HisThr: 1.046 ± 0.04	0.877HisVal: 0.877 ± 0.034	0.214HisTrp: 0.214 ± 0.016	0.95HisTyr: 0.95 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	5.653IleAla: 5.653 ± 0.085	0.724IleCys: 0.724 ± 0.031	4.923IleAsp: 4.923 ± 0.08	5.533IleGlu: 5.533 ± 0.09	3.749IlePhe: 3.749 ± 0.08	4.728IleGly: 4.728 ± 0.098	1.405IleHis: 1.405 ± 0.042	6.551IleIle: 6.551 ± 0.125	6.469IleLys: 6.469 ± 0.106	7.156IleLeu: 7.156 ± 0.116	1.392IleMet: 1.392 ± 0.048	5.244IleAsn: 5.244 ± 0.098	3.226IlePro: 3.226 ± 0.075	2.969IleGln: 2.969 ± 0.059	2.281IleArg: 2.281 ± 0.052	5.812IleSer: 5.812 ± 0.09	5.261IleThr: 5.261 ± 0.088	5.196IleVal: 5.196 ± 0.082	0.669IleTrp: 0.669 ± 0.027	3.214IleTyr: 3.214 ± 0.059	0.0IleXaa: 0.0 ± 0.0
Lys	4.828LysAla: 4.828 ± 0.089	0.433LysCys: 0.433 ± 0.026	4.216LysAsp: 4.216 ± 0.087	6.157LysGlu: 6.157 ± 0.103	3.356LysPhe: 3.356 ± 0.068	4.258LysGly: 4.258 ± 0.086	1.415LysHis: 1.415 ± 0.045	7.582LysIle: 7.582 ± 0.111	7.77LysLys: 7.77 ± 0.121	7.209LysLeu: 7.209 ± 0.105	2.265LysMet: 2.265 ± 0.052	6.64LysAsn: 6.64 ± 0.114	2.761LysPro: 2.761 ± 0.059	3.323LysGln: 3.323 ± 0.08	2.441LysArg: 2.441 ± 0.059	4.927LysSer: 4.927 ± 0.084	5.048LysThr: 5.048 ± 0.092	4.776LysVal: 4.776 ± 0.075	0.799LysTrp: 0.799 ± 0.034	3.626LysTyr: 3.626 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	5.446LeuAla: 5.446 ± 0.092	0.782LeuCys: 0.782 ± 0.027	4.663LeuAsp: 4.663 ± 0.074	5.969LeuGlu: 5.969 ± 0.102	5.254LeuPhe: 5.254 ± 0.105	5.308LeuGly: 5.308 ± 0.09	1.744LeuHis: 1.744 ± 0.049	6.671LeuIle: 6.671 ± 0.112	7.684LeuLys: 7.684 ± 0.119	8.803LeuLeu: 8.803 ± 0.145	1.924LeuMet: 1.924 ± 0.05	5.55LeuAsn: 5.55 ± 0.104	3.426LeuPro: 3.426 ± 0.071	3.956LeuGln: 3.956 ± 0.077	2.571LeuArg: 2.571 ± 0.051	6.476LeuSer: 6.476 ± 0.081	4.893LeuThr: 4.893 ± 0.097	5.372LeuVal: 5.372 ± 0.079	0.77LeuTrp: 0.77 ± 0.028	3.529LeuTyr: 3.529 ± 0.07	0.0LeuXaa: 0.0 ± 0.0
Met	1.477MetAla: 1.477 ± 0.043	0.162MetCys: 0.162 ± 0.013	0.92MetAsp: 0.92 ± 0.033	1.19MetGlu: 1.19 ± 0.041	0.97MetPhe: 0.97 ± 0.031	1.253MetGly: 1.253 ± 0.046	0.38MetHis: 0.38 ± 0.022	1.517MetIle: 1.517 ± 0.045	2.063MetLys: 2.063 ± 0.047	1.902MetLeu: 1.902 ± 0.051	0.587MetMet: 0.587 ± 0.024	1.43MetAsn: 1.43 ± 0.04	0.897MetPro: 0.897 ± 0.033	0.922MetGln: 0.922 ± 0.036	0.799MetArg: 0.799 ± 0.031	1.363MetSer: 1.363 ± 0.04	1.09MetThr: 1.09 ± 0.038	1.241MetVal: 1.241 ± 0.033	0.171MetTrp: 0.171 ± 0.014	0.82MetTyr: 0.82 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	4.157AsnAla: 4.157 ± 0.078	0.598AsnCys: 0.598 ± 0.03	3.22AsnAsp: 3.22 ± 0.069	3.945AsnGlu: 3.945 ± 0.07	3.665AsnPhe: 3.665 ± 0.073	4.134AsnGly: 4.134 ± 0.099	1.227AsnHis: 1.227 ± 0.037	5.21AsnIle: 5.21 ± 0.096	5.31AsnLys: 5.31 ± 0.089	5.968AsnLeu: 5.968 ± 0.091	1.26AsnMet: 1.26 ± 0.038	4.978AsnAsn: 4.978 ± 0.116	3.058AsnPro: 3.058 ± 0.069	2.887AsnGln: 2.887 ± 0.067	1.969AsnArg: 1.969 ± 0.048	4.312AsnSer: 4.312 ± 0.091	4.508AsnThr: 4.508 ± 0.096	3.73AsnVal: 3.73 ± 0.062	0.798AsnTrp: 0.798 ± 0.034	3.477AsnTyr: 3.477 ± 0.083	0.0AsnXaa: 0.0 ± 0.0
Pro	1.904ProAla: 1.904 ± 0.075	0.243ProCys: 0.243 ± 0.017	1.604ProAsp: 1.604 ± 0.044	2.359ProGlu: 2.359 ± 0.063	1.848ProPhe: 1.848 ± 0.047	1.471ProGly: 1.471 ± 0.051	0.572ProHis: 0.572 ± 0.027	2.76ProIle: 2.76 ± 0.057	2.741ProLys: 2.741 ± 0.063	2.705ProLeu: 2.705 ± 0.057	0.698ProMet: 0.698 ± 0.029	2.715ProAsn: 2.715 ± 0.056	0.743ProPro: 0.743 ± 0.033	1.237ProGln: 1.237 ± 0.039	0.756ProArg: 0.756 ± 0.033	2.162ProSer: 2.162 ± 0.058	2.337ProThr: 2.337 ± 0.067	2.225ProVal: 2.225 ± 0.053	0.273ProTrp: 0.273 ± 0.018	1.46ProTyr: 1.46 ± 0.046	0.0ProXaa: 0.0 ± 0.0
Gln	2.154GlnAla: 2.154 ± 0.051	0.239GlnCys: 0.239 ± 0.018	1.745GlnAsp: 1.745 ± 0.042	2.597GlnGlu: 2.597 ± 0.053	1.713GlnPhe: 1.713 ± 0.049	1.815GlnGly: 1.815 ± 0.057	0.674GlnHis: 0.674 ± 0.031	3.058GlnIle: 3.058 ± 0.067	3.749GlnLys: 3.749 ± 0.08	3.504GlnLeu: 3.504 ± 0.068	0.874GlnMet: 0.874 ± 0.031	3.132GlnAsn: 3.132 ± 0.058	1.142GlnPro: 1.142 ± 0.036	1.672GlnGln: 1.672 ± 0.052	1.129GlnArg: 1.129 ± 0.04	2.138GlnSer: 2.138 ± 0.049	2.325GlnThr: 2.325 ± 0.053	2.035GlnVal: 2.035 ± 0.051	0.347GlnTrp: 0.347 ± 0.02	1.686GlnTyr: 1.686 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	1.709ArgAla: 1.709 ± 0.044	0.19ArgCys: 0.19 ± 0.016	1.359ArgAsp: 1.359 ± 0.042	1.957ArgGlu: 1.957 ± 0.055	1.604ArgPhe: 1.604 ± 0.05	1.475ArgGly: 1.475 ± 0.044	0.483ArgHis: 0.483 ± 0.024	2.558ArgIle: 2.558 ± 0.06	2.609ArgLys: 2.609 ± 0.058	2.617ArgLeu: 2.617 ± 0.051	0.801ArgMet: 0.801 ± 0.03	1.985ArgAsn: 1.985 ± 0.05	0.834ArgPro: 0.834 ± 0.032	0.909ArgGln: 0.909 ± 0.034	0.99ArgArg: 0.99 ± 0.039	1.382ArgSer: 1.382 ± 0.04	1.619ArgThr: 1.619 ± 0.043	1.863ArgVal: 1.863 ± 0.048	0.314ArgTrp: 0.314 ± 0.019	1.331ArgTyr: 1.331 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	3.594SerAla: 3.594 ± 0.086	0.766SerCys: 0.766 ± 0.029	2.954SerAsp: 2.954 ± 0.056	3.54SerGlu: 3.54 ± 0.066	4.034SerPhe: 4.034 ± 0.079	4.371SerGly: 4.371 ± 0.109	1.104SerHis: 1.104 ± 0.042	5.632SerIle: 5.632 ± 0.094	5.373SerLys: 5.373 ± 0.096	5.806SerLeu: 5.806 ± 0.083	1.236SerMet: 1.236 ± 0.038	4.361SerAsn: 4.361 ± 0.094	1.865SerPro: 1.865 ± 0.053	2.302SerGln: 2.302 ± 0.052	1.764SerArg: 1.764 ± 0.046	4.401SerSer: 4.401 ± 0.101	4.064SerThr: 4.064 ± 0.092	4.013SerVal: 4.013 ± 0.083	0.687SerTrp: 0.687 ± 0.029	2.821SerTyr: 2.821 ± 0.069	0.0SerXaa: 0.0 ± 0.0
Thr	3.966ThrAla: 3.966 ± 0.128	0.492ThrCys: 0.492 ± 0.03	3.045ThrAsp: 3.045 ± 0.063	3.53ThrGlu: 3.53 ± 0.067	3.155ThrPhe: 3.155 ± 0.06	3.921ThrGly: 3.921 ± 0.116	1.142ThrHis: 1.142 ± 0.039	5.726ThrIle: 5.726 ± 0.102	4.518ThrLys: 4.518 ± 0.089	5.807ThrLeu: 5.807 ± 0.095	0.979ThrMet: 0.979 ± 0.035	4.235ThrAsn: 4.235 ± 0.09	2.775ThrPro: 2.775 ± 0.064	2.492ThrGln: 2.492 ± 0.059	1.489ThrArg: 1.489 ± 0.04	4.153ThrSer: 4.153 ± 0.105	4.664ThrThr: 4.664 ± 0.142	4.012ThrVal: 4.012 ± 0.103	0.59ThrTrp: 0.59 ± 0.034	2.706ThrTyr: 2.706 ± 0.081	0.0ThrXaa: 0.0 ± 0.0
Val	4.209ValAla: 4.209 ± 0.086	0.628ValCys: 0.628 ± 0.028	3.175ValAsp: 3.175 ± 0.064	3.801ValGlu: 3.801 ± 0.075	3.418ValPhe: 3.418 ± 0.07	3.569ValGly: 3.569 ± 0.067	0.958ValHis: 0.958 ± 0.033	4.515ValIle: 4.515 ± 0.083	4.622ValLys: 4.622 ± 0.075	5.933ValLeu: 5.933 ± 0.106	1.236ValMet: 1.236 ± 0.039	3.619ValAsn: 3.619 ± 0.067	2.06ValPro: 2.06 ± 0.053	1.948ValGln: 1.948 ± 0.043	1.771ValArg: 1.771 ± 0.039	4.57ValSer: 4.57 ± 0.082	3.855ValThr: 3.855 ± 0.103	4.42ValVal: 4.42 ± 0.083	0.624ValTrp: 0.624 ± 0.027	2.583ValTyr: 2.583 ± 0.053	0.0ValXaa: 0.0 ± 0.0
Trp	0.598TrpAla: 0.598 ± 0.032	0.106TrpCys: 0.106 ± 0.012	0.499TrpAsp: 0.499 ± 0.026	0.569TrpGlu: 0.569 ± 0.028	0.569TrpPhe: 0.569 ± 0.026	0.591TrpGly: 0.591 ± 0.033	0.218TrpHis: 0.218 ± 0.016	0.688TrpIle: 0.688 ± 0.028	0.834TrpLys: 0.834 ± 0.036	0.919TrpLeu: 0.919 ± 0.031	0.258TrpMet: 0.258 ± 0.018	0.779TrpAsn: 0.779 ± 0.032	0.14TrpPro: 0.14 ± 0.014	0.438TrpGln: 0.438 ± 0.022	0.305TrpArg: 0.305 ± 0.019	0.564TrpSer: 0.564 ± 0.028	0.554TrpThr: 0.554 ± 0.033	0.633TrpVal: 0.633 ± 0.029	0.106TrpTrp: 0.106 ± 0.014	0.47TrpTyr: 0.47 ± 0.028	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.383TyrAla: 2.383 ± 0.053	0.424TyrCys: 0.424 ± 0.023	2.224TyrAsp: 2.224 ± 0.056	2.294TyrGlu: 2.294 ± 0.05	2.824TyrPhe: 2.824 ± 0.055	2.529TyrGly: 2.529 ± 0.06	0.875TyrHis: 0.875 ± 0.029	3.319TyrIle: 3.319 ± 0.063	3.562TyrLys: 3.562 ± 0.064	4.043TyrLeu: 4.043 ± 0.062	0.8TyrMet: 0.8 ± 0.03	3.199TyrAsn: 3.199 ± 0.072	1.532TyrPro: 1.532 ± 0.039	1.895TyrGln: 1.895 ± 0.058	1.403TyrArg: 1.403 ± 0.043	2.952TyrSer: 2.952 ± 0.065	2.911TyrThr: 2.911 ± 0.076	2.295TyrVal: 2.295 ± 0.05	0.502TyrTrp: 0.502 ± 0.028	2.379TyrTyr: 2.379 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2701 proteins (889783 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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