Rhizobium sp. Root482

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Rhizobiaceae; Rhizobium/Agrobacterium group; Rhizobium; unclassified Rhizobium

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4853 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0T1X5T9|A0A0T1X5T9_9RHIZ Transcriptional regulator OS=Rhizobium sp. Root482 OX=1736543 GN=ASD31_05080 PE=3 SV=1
MM1 pKa = 7.49NIKK4 pKa = 10.39SLLLGSAAALAAVSGAQAADD24 pKa = 4.21AIVAAEE30 pKa = 4.47PEE32 pKa = 3.93PMEE35 pKa = 4.1YY36 pKa = 10.8VRR38 pKa = 11.84VCDD41 pKa = 4.42AFGTGYY47 pKa = 10.47FYY49 pKa = 10.57IPGTEE54 pKa = 3.81TCLKK58 pKa = 9.11VGGYY62 pKa = 9.73VRR64 pKa = 11.84TQINYY69 pKa = 9.02IDD71 pKa = 4.37NDD73 pKa = 4.23SGSDD77 pKa = 3.43AADD80 pKa = 2.9WDD82 pKa = 4.04ARR84 pKa = 11.84TKK86 pKa = 10.95GYY88 pKa = 8.86LTFAAKK94 pKa = 10.39NDD96 pKa = 3.95TEE98 pKa = 4.3IGTVSAYY105 pKa = 9.97INLEE109 pKa = 3.4AWDD112 pKa = 4.28NDD114 pKa = 4.13DD115 pKa = 5.71GNLLDD120 pKa = 4.42GAWINVAGFDD130 pKa = 4.28IGYY133 pKa = 9.63FYY135 pKa = 11.33NWWDD139 pKa = 3.64DD140 pKa = 3.77FGLSGEE146 pKa = 4.66TDD148 pKa = 2.84WADD151 pKa = 4.16NGNTLHH157 pKa = 6.36NAVRR161 pKa = 11.84YY162 pKa = 9.76VYY164 pKa = 10.73DD165 pKa = 3.9GGSFQAGLAVEE176 pKa = 4.3EE177 pKa = 4.81LSDD180 pKa = 3.62VLYY183 pKa = 10.91DD184 pKa = 4.2AGTNDD189 pKa = 4.94DD190 pKa = 4.16VGLSALIRR198 pKa = 11.84GTVGGVDD205 pKa = 3.42ALLIASYY212 pKa = 11.31DD213 pKa = 3.54FDD215 pKa = 4.97AEE217 pKa = 3.94EE218 pKa = 4.1AAFKK222 pKa = 11.0ARR224 pKa = 11.84LTADD228 pKa = 4.64LGPGTLGILGVYY240 pKa = 8.58ATDD243 pKa = 5.06PNAFWDD249 pKa = 3.43ISEE252 pKa = 4.06WSVAAEE258 pKa = 4.01YY259 pKa = 10.28KK260 pKa = 10.33IQATEE265 pKa = 3.93KK266 pKa = 10.92LFITPAVQYY275 pKa = 10.09FGDD278 pKa = 3.97YY279 pKa = 9.25GTVGTAALDD288 pKa = 3.72TLDD291 pKa = 5.18FGGDD295 pKa = 3.51DD296 pKa = 3.33AWRR299 pKa = 11.84VGITAGYY306 pKa = 9.79QITEE310 pKa = 4.19GLRR313 pKa = 11.84TLATVNYY320 pKa = 8.86TDD322 pKa = 4.8VDD324 pKa = 3.92GDD326 pKa = 5.87DD327 pKa = 4.48DD328 pKa = 4.38GTWDD332 pKa = 3.89GFLRR336 pKa = 11.84LQRR339 pKa = 11.84DD340 pKa = 3.78FF341 pKa = 4.66

Molecular weight: 36.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0T1WQU2|A0A0T1WQU2_9RHIZ EamA domain-containing protein OS=Rhizobium sp. Root482 OX=1736543 GN=ASD31_15215 PE=4 SV=1
MM1 pKa = 7.59RR2 pKa = 11.84PAVNVKK8 pKa = 10.2SVTSNVGWSILSKK21 pKa = 10.05TSTFGLKK28 pKa = 9.98FVTVPILARR37 pKa = 11.84LLSPQEE43 pKa = 3.99FGAVAVALTVVQFLAMIGGAGLTSALVIQRR73 pKa = 11.84DD74 pKa = 3.67EE75 pKa = 4.19NMEE78 pKa = 4.24TVHH81 pKa = 7.15SVFWANLAISCLMALGLFLSADD103 pKa = 3.95LFAAFLGAPEE113 pKa = 4.52AAPLLRR119 pKa = 11.84VMSFLIPLQLGGDD132 pKa = 3.57VAYY135 pKa = 10.59ALLARR140 pKa = 11.84RR141 pKa = 11.84MNFSKK146 pKa = 10.89DD147 pKa = 3.63AVWSMISEE155 pKa = 4.22SLGAVVAVVMALFGWGVWALMAQLFVSALVRR186 pKa = 11.84LCGLFAVSHH195 pKa = 4.78YY196 pKa = 10.4RR197 pKa = 11.84PRR199 pKa = 11.84LVFQLRR205 pKa = 11.84RR206 pKa = 11.84VLALSRR212 pKa = 11.84FSLGMMGSEE221 pKa = 3.96VANFITFQSPMVVVSRR237 pKa = 11.84ALGLADD243 pKa = 3.99AGAYY247 pKa = 9.43SAANRR252 pKa = 11.84FASIPNQIVLSAVMGVLFPAFSGMMDD278 pKa = 3.2DD279 pKa = 4.87RR280 pKa = 11.84EE281 pKa = 4.17RR282 pKa = 11.84RR283 pKa = 11.84SQALMFSTQVTTVLLAPMMFGLWAVAEE310 pKa = 4.0PAMRR314 pKa = 11.84VLFGPQWAYY323 pKa = 10.32AWPVLGLLALSKK335 pKa = 11.06GILTPCSTFIPYY347 pKa = 10.52LKK349 pKa = 10.64GAGHH353 pKa = 6.42GRR355 pKa = 11.84ALFWSAIIRR364 pKa = 11.84AVATTAAVAYY374 pKa = 8.23GASTGGLTGAMIWLCIVNGLTLIGYY399 pKa = 8.68SRR401 pKa = 11.84VVFRR405 pKa = 11.84ADD407 pKa = 2.83GRR409 pKa = 11.84PFLKK413 pKa = 10.63GLAISSRR420 pKa = 11.84PMLTAFVMALAVRR433 pKa = 11.84LLLNAGAEE441 pKa = 4.07LLPNPVLQILAGAACGGVIYY461 pKa = 10.61AGLIFLTEE469 pKa = 4.05RR470 pKa = 11.84PLLLKK475 pKa = 10.31IHH477 pKa = 5.9QLVKK481 pKa = 10.58KK482 pKa = 9.44PRR484 pKa = 11.84QDD486 pKa = 3.1RR487 pKa = 11.84TQGTGG492 pKa = 2.81

Molecular weight: 52.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4853

0

4853

1502100

41

3256

309.5

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.921 ± 0.043

0.813 ± 0.01

5.836 ± 0.038

5.813 ± 0.033

3.925 ± 0.024

8.483 ± 0.034

2.015 ± 0.017

5.728 ± 0.024

3.61 ± 0.027

10.019 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.016

2.774 ± 0.022

4.822 ± 0.024

3.07 ± 0.02

6.755 ± 0.034

5.717 ± 0.025

5.363 ± 0.024

7.209 ± 0.028

1.238 ± 0.014

2.254 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski