Epulopiscium sp. Nuni2H_MBin001
Average proteome isoelectric point is 5.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2185 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1V2YIK5|A0A1V2YIK5_9FIRM Uncharacterized protein OS=Epulopiscium sp. Nuni2H_MBin001 OX=1884656 GN=BEN18_04115 PE=4 SV=1
MM1 pKa = 7.38 NPRR4 pKa = 11.84 LRR6 pKa = 11.84 KK7 pKa = 9.29 EE8 pKa = 3.88 LAKK11 pKa = 10.84 LMSVSMMLGSFVPVYY26 pKa = 10.73 GYY28 pKa = 9.29 TSSTAPGVIRR38 pKa = 11.84 STSATSATTTSAIGIEE54 pKa = 4.14 DD55 pKa = 4.83 DD56 pKa = 3.97 LNNWSDD62 pKa = 3.47 VNNIVQHH69 pKa = 5.81 PSVDD73 pKa = 3.19 GSAYY77 pKa = 6.82 THH79 pKa = 5.76 YY80 pKa = 10.82 VVKK83 pKa = 8.09 TTSGAILTSYY93 pKa = 10.16 EE94 pKa = 3.98 LAPAISADD102 pKa = 4.12 NITVAYY108 pKa = 11.19 SMMSDD113 pKa = 2.79 STTNVGGIRR122 pKa = 11.84 LYY124 pKa = 11.31 NDD126 pKa = 3.1 DD127 pKa = 3.91 TVLGEE132 pKa = 4.41 VEE134 pKa = 4.58 FSGADD139 pKa = 3.44 FNANQWMDD147 pKa = 3.38 VEE149 pKa = 5.17 LVYY152 pKa = 8.28 TTDD155 pKa = 3.14 NDD157 pKa = 4.17 DD158 pKa = 3.67 NVVIDD163 pKa = 3.86 MFVDD167 pKa = 4.5 GTAVSTTYY175 pKa = 10.64 GASDD179 pKa = 4.04 VILVANKK186 pKa = 9.85 IEE188 pKa = 4.41 FFNSPSTSGAFYY200 pKa = 9.73 YY201 pKa = 10.79 DD202 pKa = 3.46 KK203 pKa = 11.3 VSVSGSDD210 pKa = 3.58 NEE212 pKa = 4.33 LLSAASANTVGEE224 pKa = 4.09 IVFFDD229 pKa = 4.09 DD230 pKa = 5.65 FSATEE235 pKa = 4.6 LSSDD239 pKa = 3.18 WTRR242 pKa = 11.84 QNTSNGTVQIEE253 pKa = 4.09 DD254 pKa = 3.26 GALNINHH261 pKa = 6.2 TVSGSSAPKK270 pKa = 9.76 AYY272 pKa = 10.76 VIFDD276 pKa = 3.75 EE277 pKa = 4.69 QSSQFGIEE285 pKa = 4.27 FDD287 pKa = 3.44 FTMINDD293 pKa = 4.39 ASPDD297 pKa = 3.5 GTTKK301 pKa = 9.74 STLSCAFGTMIGSTNITSSSSEE323 pKa = 3.63 ALRR326 pKa = 11.84 FKK328 pKa = 11.13 SNYY331 pKa = 8.44 TISGDD336 pKa = 4.28 DD337 pKa = 3.06 ITSRR341 pKa = 11.84 AFTYY345 pKa = 10.37 TDD347 pKa = 3.29 AGSTSTVNNISYY359 pKa = 10.43 EE360 pKa = 3.99 LGKK363 pKa = 9.13 TYY365 pKa = 10.38 TIAFVTDD372 pKa = 3.99 PVTQLTNIYY381 pKa = 9.86 IDD383 pKa = 3.91 GEE385 pKa = 4.45 LLVKK389 pKa = 10.57 DD390 pKa = 3.82 AAYY393 pKa = 7.5 KK394 pKa = 7.38 TTYY397 pKa = 10.72 SEE399 pKa = 4.0 ATVVDD404 pKa = 3.97 RR405 pKa = 11.84 FIFQADD411 pKa = 2.95 SSGAVNYY418 pKa = 9.57 TIDD421 pKa = 4.44 NFMVWTGDD429 pKa = 3.68 YY430 pKa = 10.45 SDD432 pKa = 4.84 KK433 pKa = 11.26 PGDD436 pKa = 3.81 SEE438 pKa = 4.23 EE439 pKa = 5.12 GEE441 pKa = 4.37 GSGDD445 pKa = 3.47 DD446 pKa = 3.53 TTEE449 pKa = 4.5 PEE451 pKa = 4.21 VSTGGFFEE459 pKa = 5.13 EE460 pKa = 5.99 DD461 pKa = 3.45 FSNWTASNSGEE472 pKa = 4.21 IISHH476 pKa = 6.99 PFTSEE481 pKa = 4.14 GYY483 pKa = 8.49 VATISGISTSSSFTYY498 pKa = 10.74 SDD500 pKa = 3.88 FSVTSEE506 pKa = 4.08 DD507 pKa = 4.37 AITVSFKK514 pKa = 10.7 MLGEE518 pKa = 4.34 DD519 pKa = 3.76 FANFSSGGIKK529 pKa = 10.11 LYY531 pKa = 10.64 EE532 pKa = 4.46 GSTEE536 pKa = 3.72 LSRR539 pKa = 11.84 INFSTGRR546 pKa = 11.84 IRR548 pKa = 11.84 YY549 pKa = 8.55 RR550 pKa = 11.84 NASGSTVTIVDD561 pKa = 4.12 TADD564 pKa = 3.64 GMQDD568 pKa = 3.61 DD569 pKa = 4.44 LWYY572 pKa = 10.09 DD573 pKa = 3.29 VSFIFYY579 pKa = 9.81 PSDD582 pKa = 3.97 DD583 pKa = 4.01 GDD585 pKa = 3.61 VMLFDD590 pKa = 4.77 VYY592 pKa = 11.29 LSGKK596 pKa = 10.43 LMGSGIEE603 pKa = 4.15 GMASNVTDD611 pKa = 3.86 GKK613 pKa = 9.5 ITIDD617 pKa = 3.66 SFRR620 pKa = 11.84 MYY622 pKa = 10.92 SDD624 pKa = 3.49 STCTGGTIYY633 pKa = 10.55 LDD635 pKa = 3.64 EE636 pKa = 5.74 IMVQEE641 pKa = 4.42 GKK643 pKa = 10.69 VIPSEE648 pKa = 4.23 SQPASPYY655 pKa = 10.83 NYY657 pKa = 9.96 LEE659 pKa = 4.22 EE660 pKa = 4.56 SAEE663 pKa = 3.98 NWTLATSSEE672 pKa = 4.27 LDD674 pKa = 3.33 VSPFRR679 pKa = 11.84 DD680 pKa = 3.5 MEE682 pKa = 5.41 DD683 pKa = 5.25 DD684 pKa = 3.23 IVLYY688 pKa = 10.47 AGKK691 pKa = 10.32 YY692 pKa = 8.05 NNIYY696 pKa = 9.54 TEE698 pKa = 4.36 SFSATDD704 pKa = 4.62 AITINFDD711 pKa = 3.46 VVSAATEE718 pKa = 4.26 TVSGIVTNKK727 pKa = 9.69 VSGDD731 pKa = 3.5 VILNDD736 pKa = 3.41 RR737 pKa = 11.84 TNDD740 pKa = 2.99 IFTLTFDD747 pKa = 4.42 GEE749 pKa = 4.21 EE750 pKa = 4.16 LKK752 pKa = 11.14 AGAQTLATGIDD763 pKa = 3.07 AFYY766 pKa = 10.15 WNDD769 pKa = 2.55 ISIVLKK775 pKa = 10.68 EE776 pKa = 4.35 SGADD780 pKa = 3.24 VYY782 pKa = 11.8 LNGEE786 pKa = 4.21 KK787 pKa = 10.69 VGDD790 pKa = 4.0 NLSTSATSVGFEE802 pKa = 4.2 TINFKK807 pKa = 10.83 SSNSYY812 pKa = 10.55 EE813 pKa = 3.93 SDD815 pKa = 3.72 FIFNDD820 pKa = 2.7 ITVRR824 pKa = 11.84 TTEE827 pKa = 4.35 VIPSAGEE834 pKa = 3.72 PVKK837 pKa = 10.52 AYY839 pKa = 10.94 YY840 pKa = 9.56 PDD842 pKa = 3.65 QEE844 pKa = 4.34 TDD846 pKa = 3.35 KK847 pKa = 11.07 PDD849 pKa = 2.98 ISHH852 pKa = 6.04 YY853 pKa = 10.74 LKK855 pKa = 10.08 TIEE858 pKa = 5.57 AMGTLDD864 pKa = 4.23 AAYY867 pKa = 9.19 PEE869 pKa = 4.32 PNIYY873 pKa = 10.63 NSGLDD878 pKa = 3.41 GYY880 pKa = 9.76 EE881 pKa = 3.57 IVGPEE886 pKa = 4.07 VVAGATTHH894 pKa = 5.95 NVQDD898 pKa = 4.06 ALDD901 pKa = 4.0 AAGVADD907 pKa = 4.95 GEE909 pKa = 4.68 DD910 pKa = 3.49 VTDD913 pKa = 3.96 VVQSLIDD920 pKa = 3.43 NAEE923 pKa = 4.15 YY924 pKa = 11.04 GDD926 pKa = 3.77 TVYY929 pKa = 10.59 FPQGTYY935 pKa = 11.07 NFITTATNAITLKK948 pKa = 10.85 DD949 pKa = 3.76 GVSLIGEE956 pKa = 4.67 GEE958 pKa = 4.06 DD959 pKa = 3.87 QSILKK964 pKa = 10.15 ISNTSSATSAYY975 pKa = 9.76 FIRR978 pKa = 11.84 GTGVTNLAIKK988 pKa = 10.65 DD989 pKa = 3.77 LTLTSAYY996 pKa = 10.33 YY997 pKa = 10.95 GDD999 pKa = 4.4 YY1000 pKa = 10.46 SDD1002 pKa = 4.35 SSIYY1006 pKa = 10.38 AQLGTNTTRR1015 pKa = 11.84 EE1016 pKa = 4.44 GNMSHH1021 pKa = 7.48 GIYY1024 pKa = 10.13 LDD1026 pKa = 4.13 RR1027 pKa = 11.84 NTSTGQPCQYY1037 pKa = 10.55 IEE1039 pKa = 4.17 IEE1041 pKa = 4.21 NVTVEE1046 pKa = 4.04 IFKK1049 pKa = 10.63 TMAIALRR1056 pKa = 11.84 NTQDD1060 pKa = 3.25 VRR1062 pKa = 11.84 VKK1064 pKa = 10.55 GCTVQYY1070 pKa = 7.92 PTDD1073 pKa = 3.61 VAGGGAGYY1081 pKa = 10.54 GVTVQGTADD1090 pKa = 3.59 TDD1092 pKa = 3.19 NWGQPYY1098 pKa = 10.94 DD1099 pKa = 3.67 SMNNIVEE1106 pKa = 4.32 DD1107 pKa = 3.76 CTLIGLRR1114 pKa = 11.84 HH1115 pKa = 6.6 GILLQYY1121 pKa = 7.77 YY1122 pKa = 6.54 THH1124 pKa = 6.87 NNLVINNKK1132 pKa = 9.46 LVGMGYY1138 pKa = 10.4 GAIDD1142 pKa = 3.2 LHH1144 pKa = 9.04 GEE1146 pKa = 3.96 DD1147 pKa = 4.62 EE1148 pKa = 4.6 YY1149 pKa = 11.88 LNEE1152 pKa = 3.8 IAFNYY1157 pKa = 9.34 VADD1160 pKa = 4.02 THH1162 pKa = 6.07 WGGGIEE1168 pKa = 4.72 LGNSGAGHH1176 pKa = 7.29 DD1177 pKa = 4.4 LAGPLNFVHH1186 pKa = 6.59 NNTVDD1191 pKa = 3.05 GGLRR1195 pKa = 11.84 GIDD1198 pKa = 3.48 IEE1200 pKa = 5.05 FGTPDD1205 pKa = 4.21 TIVQNNTVKK1214 pKa = 10.92 NISNTNNTSDD1224 pKa = 3.73 SNGAAIRR1231 pKa = 11.84 IRR1233 pKa = 11.84 NAEE1236 pKa = 4.04 RR1237 pKa = 11.84 TSVSGNTFTDD1247 pKa = 3.22 NVTGILFDD1255 pKa = 3.86 TDD1257 pKa = 4.0 SGYY1260 pKa = 11.29 SWGDD1264 pKa = 3.22 LSDD1267 pKa = 3.57 WGAGTPFQSDD1277 pKa = 3.67 VFDD1280 pKa = 3.7 NTVSGNGTDD1289 pKa = 4.13 YY1290 pKa = 11.28 ILTAGNEE1297 pKa = 4.11 NTVLGQTADD1306 pKa = 3.37 GTIVVEE1312 pKa = 4.79 KK1313 pKa = 10.27 SANAHH1318 pKa = 5.71 LVALEE1323 pKa = 3.79 PAEE1326 pKa = 5.14 AEE1328 pKa = 4.1 FTTAFIPYY1336 pKa = 8.07 ITDD1339 pKa = 3.05 YY1340 pKa = 11.19 TIFVPGNEE1348 pKa = 4.09 LTLTPHH1354 pKa = 7.19 ASASNVGKK1362 pKa = 10.37 IVINGAEE1369 pKa = 4.19 VPNGEE1374 pKa = 4.98 SIILTDD1380 pKa = 3.53 TKK1382 pKa = 11.06 VDD1384 pKa = 3.2 IVVTSEE1390 pKa = 4.42 DD1391 pKa = 3.46 GANTKK1396 pKa = 9.53 TYY1398 pKa = 10.59 SIAATYY1404 pKa = 10.11 AAGDD1408 pKa = 4.01 DD1409 pKa = 4.01 YY1410 pKa = 12.07 VPVQNVQLQGSEE1422 pKa = 3.8 PTIPNNDD1429 pKa = 3.22 SYY1431 pKa = 11.49 QLEE1434 pKa = 4.53 VSVLPTNATNQKK1446 pKa = 9.82 LIWEE1450 pKa = 4.97 IIDD1453 pKa = 4.09 GNDD1456 pKa = 4.34 IMSIDD1461 pKa = 3.9 QNGLLTPLTGAGDD1474 pKa = 3.83 VVVRR1478 pKa = 11.84 ATSDD1482 pKa = 3.22 EE1483 pKa = 4.36 SEE1485 pKa = 4.04 SMYY1488 pKa = 11.36 AEE1490 pKa = 3.88 FTVTVIEE1497 pKa = 4.81 APTLPDD1503 pKa = 4.14 HH1504 pKa = 6.78 YY1505 pKa = 11.24 DD1506 pKa = 3.25 EE1507 pKa = 4.63 TAEE1510 pKa = 3.98 QLSVVAVTDD1519 pKa = 3.52 SGNDD1523 pKa = 3.35 GNGPDD1528 pKa = 4.57 NIADD1532 pKa = 3.87 GDD1534 pKa = 4.11 LTTRR1538 pKa = 11.84 WSNNEE1543 pKa = 3.52 KK1544 pKa = 9.97 PGYY1547 pKa = 9.21 VALEE1551 pKa = 4.12 LADD1554 pKa = 3.73 SAEE1557 pKa = 4.01 IAHH1560 pKa = 6.6 VKK1562 pKa = 9.09 VAWNSGNTRR1571 pKa = 11.84 KK1572 pKa = 10.27 SYY1574 pKa = 10.75 FNIYY1578 pKa = 8.26 VTDD1581 pKa = 3.91 TAPDD1585 pKa = 3.16 IASADD1590 pKa = 3.78 NVDD1593 pKa = 3.45 TSNWNLVYY1601 pKa = 10.39 TDD1603 pKa = 3.8 EE1604 pKa = 4.45 TDD1606 pKa = 3.93 GYY1608 pKa = 8.85 ATSSGLMTLPEE1619 pKa = 4.16 ACVFDD1624 pKa = 4.16 EE1625 pKa = 5.16 PIEE1628 pKa = 4.23 GKK1630 pKa = 9.97 YY1631 pKa = 10.51 VIYY1634 pKa = 10.48 EE1635 pKa = 3.99 GLGNEE1640 pKa = 3.82 DD1641 pKa = 3.83 SAWNSVLEE1649 pKa = 4.11 IEE1651 pKa = 5.13 VYY1653 pKa = 10.68 GHH1655 pKa = 7.23 DD1656 pKa = 4.81 GEE1658 pKa = 5.01 TTTPTPDD1665 pKa = 3.22 EE1666 pKa = 5.39 DD1667 pKa = 5.1 KK1668 pKa = 11.47 DD1669 pKa = 4.43 DD1670 pKa = 4.37 DD1671 pKa = 3.98 AQEE1674 pKa = 4.06 NEE1676 pKa = 4.65 IIYY1679 pKa = 8.44 STTFDD1684 pKa = 3.99 TLDD1687 pKa = 4.51 SSWTTQNTSYY1697 pKa = 11.61 DD1698 pKa = 3.82 NVSAQAVDD1706 pKa = 3.56 GALQIVDD1713 pKa = 4.51 EE1714 pKa = 4.67 NDD1716 pKa = 3.26 EE1717 pKa = 4.23 QQVKK1721 pKa = 10.84 AFVTFDD1727 pKa = 3.26 AQTEE1731 pKa = 4.3 PVTIQYY1737 pKa = 10.55 DD1738 pKa = 3.48 FRR1740 pKa = 11.84 IDD1742 pKa = 3.38 TNEE1745 pKa = 3.74 YY1746 pKa = 9.28 ATTLTSAFGKK1756 pKa = 10.64 SSITSSSNEE1765 pKa = 3.53 ALRR1768 pKa = 11.84 FTTTGSTAGEE1778 pKa = 3.85 QQFRR1782 pKa = 11.84 YY1783 pKa = 8.25 TNGVDD1788 pKa = 4.03 ADD1790 pKa = 4.05 GATINAVIDD1799 pKa = 3.7 GAIEE1803 pKa = 3.52 IDD1805 pKa = 2.95 KK1806 pKa = 9.73 WYY1808 pKa = 10.79 NIAFTTDD1815 pKa = 2.98 PTTGKK1820 pKa = 8.05 TVIYY1824 pKa = 10.28 IDD1826 pKa = 3.65 GTQVAVSEE1834 pKa = 4.69 SYY1836 pKa = 11.19 YY1837 pKa = 10.8 KK1838 pKa = 10.8 DD1839 pKa = 3.92 GIDD1842 pKa = 4.64 EE1843 pKa = 3.91 ITNYY1847 pKa = 9.69 IFQTGGSAFAAYY1859 pKa = 10.2 SVDD1862 pKa = 3.14 NFIVYY1867 pKa = 8.88 TGEE1870 pKa = 3.81 FNAEE1874 pKa = 3.01 IWDD1877 pKa = 3.77 NVEE1880 pKa = 3.97 EE1881 pKa = 4.24 EE1882 pKa = 4.4 EE1883 pKa = 5.3 EE1884 pKa = 4.48 EE1885 pKa = 4.39 DD1886 pKa = 3.61 TSHH1889 pKa = 7.51 PGLIKK1894 pKa = 10.63 PSDD1897 pKa = 3.94 VLTLDD1902 pKa = 3.41 DD1903 pKa = 4.98 WKK1905 pKa = 10.97 LQLPIVSGTGILEE1918 pKa = 4.04 IQKK1921 pKa = 10.78 DD1922 pKa = 3.88 SLDD1925 pKa = 4.24 GYY1927 pKa = 7.13 SHH1929 pKa = 7.57 KK1930 pKa = 10.98 DD1931 pKa = 3.13 YY1932 pKa = 10.68 FYY1934 pKa = 10.8 YY1935 pKa = 10.75 DD1936 pKa = 3.65 EE1937 pKa = 6.19 DD1938 pKa = 3.93 IEE1940 pKa = 5.27 GVVFYY1945 pKa = 10.96 CPVDD1949 pKa = 3.49 GGKK1952 pKa = 7.73 TANTVYY1958 pKa = 10.77 SRR1960 pKa = 11.84 TEE1962 pKa = 3.66 LRR1964 pKa = 11.84 EE1965 pKa = 3.93 MIGTSTADD1973 pKa = 2.9 NWDD1976 pKa = 3.39 FAGTHH1981 pKa = 6.13 ILNTSQKK1988 pKa = 8.12 VTEE1991 pKa = 4.39 VPSNGNVVTSQIHH2004 pKa = 6.06 SIYY2007 pKa = 9.71 PDD2009 pKa = 3.65 GSNGPALIKK2018 pKa = 10.34 VQYY2021 pKa = 9.1 EE2022 pKa = 4.37 GNDD2025 pKa = 3.22 EE2026 pKa = 4.77 RR2027 pKa = 11.84 ILVMFKK2033 pKa = 10.85 SSTDD2037 pKa = 3.18 DD2038 pKa = 2.95 TAADD2042 pKa = 3.05 VKK2044 pKa = 10.83 MYY2046 pKa = 11.26 VNDD2049 pKa = 3.61 IKK2051 pKa = 10.95 LDD2053 pKa = 3.62 QVFDD2057 pKa = 4.05 TEE2059 pKa = 4.24 IKK2061 pKa = 10.77 LIDD2064 pKa = 4.08 GYY2066 pKa = 11.62 GYY2068 pKa = 9.03 VTVTSEE2074 pKa = 4.2 GVTTEE2079 pKa = 4.05 LSYY2082 pKa = 11.67 DD2083 pKa = 3.54 FMGIDD2088 pKa = 3.8 PTWDD2092 pKa = 3.26 DD2093 pKa = 3.93 KK2094 pKa = 11.51 YY2095 pKa = 11.75 NNYY2098 pKa = 9.49 FKK2100 pKa = 10.79 AGNYY2104 pKa = 9.03 IQDD2107 pKa = 4.03 SVQDD2111 pKa = 4.29 DD2112 pKa = 3.89 PDD2114 pKa = 5.19 GPTEE2118 pKa = 4.08 GATVIMYY2125 pKa = 10.59 SLEE2128 pKa = 4.34 TYY2130 pKa = 10.5 HH2131 pKa = 8.03 SDD2133 pKa = 2.94 EE2134 pKa = 4.48 VEE2136 pKa = 4.06 DD2137 pKa = 4.14 VKK2139 pKa = 11.01 IEE2141 pKa = 3.96 SLDD2144 pKa = 3.64 FVRR2147 pKa = 11.84 SEE2149 pKa = 4.06 IEE2151 pKa = 3.85 LALGDD2156 pKa = 4.12 TTTLQPVFTPVDD2168 pKa = 3.66 TYY2170 pKa = 11.62 NKK2172 pKa = 9.79 KK2173 pKa = 8.69 LTYY2176 pKa = 9.52 TVTEE2180 pKa = 4.09 GAEE2183 pKa = 4.06 NVAVGIDD2190 pKa = 3.75 GTILGLNKK2198 pKa = 10.57 GEE2200 pKa = 4.01 ATILATSQSDD2210 pKa = 3.81 GSVTAEE2216 pKa = 3.66 IKK2218 pKa = 9.58 VTVVDD2223 pKa = 3.71 EE2224 pKa = 4.62 AEE2226 pKa = 4.06 EE2227 pKa = 4.32 VEE2229 pKa = 4.38 AEE2231 pKa = 5.55 LIASWDD2237 pKa = 3.64 FSKK2240 pKa = 11.14 ALSEE2244 pKa = 4.22 YY2245 pKa = 10.52 NDD2247 pKa = 3.66 LTISVPDD2254 pKa = 3.29 SGATVEE2260 pKa = 4.93 IVDD2263 pKa = 5.31 GYY2265 pKa = 11.01 LQVIDD2270 pKa = 4.4 DD2271 pKa = 4.41 TLLASTSALASFPEE2285 pKa = 4.26 QTGTTTISYY2294 pKa = 10.02 KK2295 pKa = 10.07 IRR2297 pKa = 11.84 LDD2299 pKa = 3.51 EE2300 pKa = 4.63 NRR2302 pKa = 11.84 VKK2304 pKa = 10.87 DD2305 pKa = 3.68 ADD2307 pKa = 4.05 SNKK2310 pKa = 9.35 PLSSVIYY2317 pKa = 10.38 VDD2319 pKa = 3.63 VAHH2322 pKa = 6.75 NEE2324 pKa = 4.02 NMSSSVTRR2332 pKa = 11.84 VRR2334 pKa = 11.84 NTGDD2338 pKa = 3.44 YY2339 pKa = 8.87 NTEE2342 pKa = 3.98 TEE2344 pKa = 4.23 GLDD2347 pKa = 3.37 NQRR2350 pKa = 11.84 WQVTNNYY2357 pKa = 10.03 SDD2359 pKa = 3.69 VDD2361 pKa = 4.09 MNEE2364 pKa = 3.95 DD2365 pKa = 3.37 AAVVTNDD2372 pKa = 2.88 WQEE2375 pKa = 3.86 FTIITTPNNGTGLANTTDD2393 pKa = 3.58 VYY2395 pKa = 11.04 IDD2397 pKa = 3.61 GVMVGYY2403 pKa = 10.45 QLTDD2407 pKa = 3.22 NNVADD2412 pKa = 4.07 SINRR2416 pKa = 11.84 VRR2418 pKa = 11.84 FMTGTKK2424 pKa = 10.5 DD2425 pKa = 4.75 LIDD2428 pKa = 4.27 FSLDD2432 pKa = 3.37 DD2433 pKa = 3.69 VKK2435 pKa = 11.36 VYY2437 pKa = 10.9 SGAKK2441 pKa = 10.23 LPDD2444 pKa = 4.58 NITNAAPTQVTVTAEE2459 pKa = 4.05 TEE2461 pKa = 4.09 EE2462 pKa = 4.42 LVVGDD2467 pKa = 4.12 TLQLIAAVLPADD2479 pKa = 4.38 ASPDD2483 pKa = 3.33 VTYY2486 pKa = 10.82 EE2487 pKa = 4.09 VIDD2490 pKa = 4.04 GDD2492 pKa = 4.37 TVVGVSKK2499 pKa = 10.97 NGLVIAKK2506 pKa = 10.22 AEE2508 pKa = 4.09 GTATIRR2514 pKa = 11.84 VATKK2518 pKa = 10.27 ADD2520 pKa = 3.25 EE2521 pKa = 4.26 SVYY2524 pKa = 10.24 FDD2526 pKa = 3.9 FVITVTADD2534 pKa = 3.17 STDD2537 pKa = 3.47 EE2538 pKa = 4.23 GDD2540 pKa = 3.56 GDD2542 pKa = 4.23 GNEE2545 pKa = 4.37 GGGNEE2550 pKa = 4.35 GGGDD2554 pKa = 3.75 GNEE2557 pKa = 4.24 GGGNEE2562 pKa = 4.35 GGGDD2566 pKa = 3.81 GNEE2569 pKa = 4.18 GDD2571 pKa = 5.57 GDD2573 pKa = 4.2 DD2574 pKa = 5.48 EE2575 pKa = 6.18 DD2576 pKa = 6.7 DD2577 pKa = 4.06 EE2578 pKa = 7.11 DD2579 pKa = 5.03 EE2580 pKa = 6.6 DD2581 pKa = 5.47 EE2582 pKa = 6.56 DD2583 pKa = 5.73 DD2584 pKa = 6.41 DD2585 pKa = 5.33 EE2586 pKa = 7.62 DD2587 pKa = 4.96 GGNEE2591 pKa = 3.95 GGGNEE2596 pKa = 4.22 GGGNEE2601 pKa = 4.67 GDD2603 pKa = 4.73 GDD2605 pKa = 5.26 DD2606 pKa = 4.76 EE2607 pKa = 6.91 DD2608 pKa = 6.17 EE2609 pKa = 5.54 DD2610 pKa = 6.2 DD2611 pKa = 5.44 EE2612 pKa = 7.53 DD2613 pKa = 6.66 DD2614 pKa = 5.36 GDD2616 pKa = 4.52 EE2617 pKa = 5.4 DD2618 pKa = 6.42 DD2619 pKa = 5.98 DD2620 pKa = 5.96 DD2621 pKa = 5.1 SSSDD2625 pKa = 3.1 IVVEE2629 pKa = 4.94 DD2630 pKa = 3.85 EE2631 pKa = 4.16 VNDD2634 pKa = 4.12 YY2635 pKa = 11.0 IEE2637 pKa = 4.73 SLTDD2641 pKa = 3.41 AEE2643 pKa = 4.1 LDD2645 pKa = 3.64 EE2646 pKa = 5.12 YY2647 pKa = 10.84 IEE2649 pKa = 4.32 EE2650 pKa = 4.12 FDD2652 pKa = 4.04 VVVGEE2657 pKa = 4.21 TMTDD2661 pKa = 2.74 ILDD2664 pKa = 3.77 NVAVDD2669 pKa = 3.84 EE2670 pKa = 4.43 TDD2672 pKa = 3.24 VNAFTEE2678 pKa = 4.24 ILNQTLSILEE2688 pKa = 4.12 ALQNLVTEE2696 pKa = 4.72 IFANIPTLANSDD2708 pKa = 3.63 VKK2710 pKa = 10.35 EE2711 pKa = 4.09 AKK2713 pKa = 9.67 IDD2715 pKa = 3.76 PDD2717 pKa = 3.65 TAKK2720 pKa = 10.68 FGEE2723 pKa = 4.23 RR2724 pKa = 11.84 KK2725 pKa = 9.67 GNKK2728 pKa = 8.07 IQYY2731 pKa = 7.75 TFYY2734 pKa = 10.07 VTVEE2738 pKa = 3.74 YY2739 pKa = 9.62 TVNVNSRR2746 pKa = 11.84 TNEE2749 pKa = 3.84 TATMEE2754 pKa = 4.14 IGPFSTWVSVDD2765 pKa = 2.89 NTAPTFSEE2773 pKa = 4.0 QHH2775 pKa = 6.29 EE2776 pKa = 4.42 DD2777 pKa = 3.36 TYY2779 pKa = 11.51 SILVGDD2785 pKa = 4.71 SFTLPVVTATDD2796 pKa = 3.36 ASGDD2800 pKa = 3.88 DD2801 pKa = 3.69 VEE2803 pKa = 4.8 VTSEE2807 pKa = 3.53 ITRR2810 pKa = 11.84 NGTVVDD2816 pKa = 5.29 SIDD2819 pKa = 3.31 TDD2821 pKa = 3.66 TAGTYY2826 pKa = 9.76 IVTYY2830 pKa = 8.84 TATDD2834 pKa = 3.48 ADD2836 pKa = 4.41 GNTAEE2841 pKa = 4.24 TTITVRR2847 pKa = 11.84 VLLDD2851 pKa = 3.28 GSITGDD2857 pKa = 3.35 YY2858 pKa = 10.45 NIPTFSYY2865 pKa = 10.78 PDD2867 pKa = 3.58 LSNSNTIDD2875 pKa = 4.68 DD2876 pKa = 4.75 EE2877 pKa = 5.55 DD2878 pKa = 6.26 DD2879 pKa = 3.68 SDD2881 pKa = 5.64 EE2882 pKa = 4.8 EE2883 pKa = 6.18 DD2884 pKa = 4.43 DD2885 pKa = 6.59 DD2886 pKa = 6.03 SDD2888 pKa = 5.03 EE2889 pKa = 5.14 EE2890 pKa = 5.17 DD2891 pKa = 5.85 DD2892 pKa = 5.4 SDD2894 pKa = 5.18 EE2895 pKa = 5.87 DD2896 pKa = 5.26 DD2897 pKa = 4.87 DD2898 pKa = 6.59 SEE2900 pKa = 4.98 DD2901 pKa = 4.28 LEE2903 pKa = 4.53 EE2904 pKa = 5.9 NEE2906 pKa = 5.43 DD2907 pKa = 3.84 DD2908 pKa = 3.53 VDD2910 pKa = 4.21 YY2911 pKa = 11.66 VSLINVEE2918 pKa = 4.87 EE2919 pKa = 4.23 IFTDD2923 pKa = 3.35 INKK2926 pKa = 9.96 SAWYY2930 pKa = 9.29 YY2931 pKa = 10.95 SDD2933 pKa = 2.9 IAYY2936 pKa = 10.17 VYY2938 pKa = 10.94 SLGLIKK2944 pKa = 10.57 GVTEE2948 pKa = 4.05 TEE2950 pKa = 4.35 YY2951 pKa = 11.01 EE2952 pKa = 4.03 PSSYY2956 pKa = 9.95 ATRR2959 pKa = 11.84 AQLATILNRR2968 pKa = 11.84 IAGNVTVTSGNEE2980 pKa = 3.77 FTDD2983 pKa = 3.92 VVAGAWYY2990 pKa = 9.9 EE2991 pKa = 4.22 SAINWAVATGVYY3003 pKa = 9.92 NGYY3006 pKa = 10.31 EE3007 pKa = 4.27 DD3008 pKa = 3.73 GTFRR3012 pKa = 11.84 PSEE3015 pKa = 4.42 SITRR3019 pKa = 11.84 EE3020 pKa = 3.62 QLAVIMFNFANSQGYY3035 pKa = 5.85 TTAANASLNGFTDD3048 pKa = 3.83 SNEE3051 pKa = 3.92 VASWATTAVQWTVANGIIGGKK3072 pKa = 9.85 DD3073 pKa = 3.29 NNRR3076 pKa = 11.84 LDD3078 pKa = 4.1 PKK3080 pKa = 11.2 GEE3082 pKa = 3.81 ATRR3085 pKa = 11.84 AEE3087 pKa = 3.79 IAAIITRR3094 pKa = 11.84 FLNN3097 pKa = 3.52
Molecular weight: 334.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.643
IPC_protein 3.706
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.617
Sillero 3.821
Patrickios 1.24
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.72
Protein with the highest isoelectric point:
>tr|A0A1V2YHG0|A0A1V2YHG0_9FIRM Uncharacterized protein OS=Epulopiscium sp. Nuni2H_MBin001 OX=1884656 GN=BEN18_05530 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.85 KK9 pKa = 7.62 RR10 pKa = 11.84 QRR12 pKa = 11.84 SKK14 pKa = 9.69 EE15 pKa = 3.5 HH16 pKa = 6.13 GFRR19 pKa = 11.84 KK20 pKa = 9.94 RR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 TGRR28 pKa = 11.84 AVIARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.13 GRR39 pKa = 11.84 KK40 pKa = 9.04 KK41 pKa = 9.67 LTAA44 pKa = 4.2
Molecular weight: 5.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.444
IPC2_protein 10.965
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.457
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.179
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.076
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2185
0
2185
784033
37
5982
358.8
40.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.919 ± 0.068
1.116 ± 0.022
6.181 ± 0.065
6.961 ± 0.054
4.021 ± 0.04
6.216 ± 0.053
1.661 ± 0.029
8.958 ± 0.056
6.312 ± 0.072
9.085 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.656 ± 0.027
5.598 ± 0.044
3.077 ± 0.037
3.488 ± 0.035
3.185 ± 0.042
6.222 ± 0.053
6.243 ± 0.101
7.124 ± 0.051
0.733 ± 0.014
4.242 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here